rnase inhibitor (Promega)
90
Structured Review
Promega
rnase inhibitor
Rnase Inhibitor, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sds+based+lysis+buffer+component/rnasin/pmc09531950-411-8-33
Average 90 stars, based on 1 article reviews
Rnase Inhibitor, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sds+based+lysis+buffer+component/rnasin/pmc09531950-411-8-33
Average 90 stars, based on 1 article reviews
rnase inhibitor - by Bioz Stars,
2026-11
90/100 stars
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Magnetic Beads:Article Title: Single-cell multiomic analysis reveals methylome and transcriptome deviations following oocyte maturation in vitro Article Snippet: Briefly, we used magnetic beads (MyOne C1, Life Technologies) annealed to Smart-seq2 oligo-dTs to capture polyadenylated mRNA from the single oocyte lysate. .. DNA from the remaining lysate was transferred to a separate tube, while the magnetic beads were washed three times in a solution containing 1×FSS buffer (Superscript II, Invitrogen, UK), 10 mM DTT, 0.005% Tween-20 (Sigma), and 0.4 U/μL of Article Title: Single-cell multiomic analysis reveals methylome and transcriptome deviations following oocyte maturation in vitro Article Snippet: .. The mRNA attached to the magnetic beads was immediately processed for cDNA conversion by resuspending the beads in a 10 μL reverse transcriptase mastermix (100 U SuperScript II (Invitrogen), 10 U Extraction:Article Title: Deciphering the role of CAPZA2 in neurodevelopmental disorders: insights from mouse models. Article Snippet: .. All buffers and gradient solutions for nuclei extraction contained 60 U/mL Article Title: Decreasing the intrinsically disordered protein α-synuclein levels by targeting its structured mRNA with a ribonuclease targeting chimera Article Snippet: .. After centrifugation at 5,000 rpm for 5 min, the supernatant was removed, and the cell pellets were lysed with 250 μL of ice-cold Polysome Extraction Buffer [10 mM Tris, pH 7.5, 10 mM NaCl, 10 mM MgCl2, 1% (v/v) Triton X-100, 1% (w/v) sodium deoxycholate, 1 mM dithiothreitol (DTT) supplemented with 100 μg/mL CHX and 0.2 U/μL Reverse Transcription:Article Title: Single-cell multiomic analysis reveals methylome and transcriptome deviations following oocyte maturation in vitro Article Snippet: .. The mRNA attached to the magnetic beads was immediately processed for cDNA conversion by resuspending the beads in a 10 μL reverse transcriptase mastermix (100 U SuperScript II (Invitrogen), 10 U Article Title: Type-I interferons in Vulvovaginal Candidiasis: Mechanism of epithelial early defense and immune regulation against Candida albicans. Article Snippet: Vulvovaginal candidiasis (VVC) is a mucosal infection predominantly caused by Candida albicans, affecting over three-quarters of immunocompetent women worldwide.. While the female genital tract mucosa is the primary defense against the fungus, the specific immune mechanisms involved in this host-pathogen interaction remain largely unknown.. In this study, we explored the relevance of type-I interferons (IFNs-I) pathway using both in vitro and in vivo models of VVC. Centrifugation:Article Title: Decreasing the intrinsically disordered protein α-synuclein levels by targeting its structured mRNA with a ribonuclease targeting chimera Article Snippet: .. After centrifugation at 5,000 rpm for 5 min, the supernatant was removed, and the cell pellets were lysed with 250 μL of ice-cold Polysome Extraction Buffer [10 mM Tris, pH 7.5, 10 mM NaCl, 10 mM MgCl2, 1% (v/v) Triton X-100, 1% (w/v) sodium deoxycholate, 1 mM dithiothreitol (DTT) supplemented with 100 μg/mL CHX and 0.2 U/μL |