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rneasy minikit  (Qiagen)


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    Structured Review

    Qiagen rneasy minikit
    Rneasy Minikit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 241714 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/scriptseq+v%2E2+kit/RNeasy+Mini+Kit/pmc09769816-161-5-7
    Average 99 stars, based on 241714 article reviews
    rneasy minikit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: G-quadruplex-binding ligand RHPS4 reduces Plasmodium falciparum mitochondrial DNA level providing a novel antimalarial strategy.
    Article Snippet: .. To quantify the relative abundance of mRNA or rRNA by real-time quantitative polymerase chain reaction (qRT-PCR), total RNA was isolated from parasite pellets (after RBC lysis by saponin treatment) using the RNeasy Mini kit (Qiagen, Germantown, MD). .. Next, cDNA was synthesized using the iTaq Universal SYBR Green One-Step Kit (Bio-Rad) with specific primers (Table S1), 20 ng/reaction, and 300 nM primers in 10 μL of final reaction volume in a thermal cycler (Bio-Rad) with the following parameters: 50°C for 10 min, followed by 95°C for 1 min, and 39 cycles at 95°C for 10 s and 60°C for 30 s, followed by a melting curve from 65°C to 95°C.

    Isolation:

    Article Title: G-quadruplex-binding ligand RHPS4 reduces Plasmodium falciparum mitochondrial DNA level providing a novel antimalarial strategy.
    Article Snippet: .. To quantify the relative abundance of mRNA or rRNA by real-time quantitative polymerase chain reaction (qRT-PCR), total RNA was isolated from parasite pellets (after RBC lysis by saponin treatment) using the RNeasy Mini kit (Qiagen, Germantown, MD). .. Next, cDNA was synthesized using the iTaq Universal SYBR Green One-Step Kit (Bio-Rad) with specific primers (Table S1), 20 ng/reaction, and 300 nM primers in 10 μL of final reaction volume in a thermal cycler (Bio-Rad) with the following parameters: 50°C for 10 min, followed by 95°C for 1 min, and 39 cycles at 95°C for 10 s and 60°C for 30 s, followed by a melting curve from 65°C to 95°C.

    Article Title: Variants in CEP135 Cause Congenital Microcephaly and Primary Ciliary Dyskinesia
    Article Snippet: .. Reverse transcription polymerase chain reaction (RT‐PCR) for CEP135 exons 6–9 was performed on RNA from HNECs isolated using a RNeasy Kit (Qiagen, Germantown, MD, USA) as per manufacturer's instructions. .. First strand cDNA was synthesized using SuperScript II Reverse Transcriptase kit (Thermo Fisher Scientific).

    Article Title: Integrated morphological and transcriptomic analysis of bone disorders in aldosterone-salt hypertension.
    Article Snippet: .. Total RNA was isolated from femoral bone tissue and kidney cortical tissue using the RNeasy Mini Kit (Qiagen, Hilden, Germany). .. RNA quantity and quality were assessed using a NanoDrop-2000 spectrophotometer (Thermo Fisher Scientific) and a TapeStation system (Agilent Technologies, Santa Clara, CA, USA), respectively.

    Article Title: The Role of Aldehyde Dehydrogenase 2 in Hematopoiesis is Stress-Severity Dependent.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Parasite:

    Article Title: G-quadruplex-binding ligand RHPS4 reduces Plasmodium falciparum mitochondrial DNA level providing a novel antimalarial strategy.
    Article Snippet: .. To quantify the relative abundance of mRNA or rRNA by real-time quantitative polymerase chain reaction (qRT-PCR), total RNA was isolated from parasite pellets (after RBC lysis by saponin treatment) using the RNeasy Mini kit (Qiagen, Germantown, MD). .. Next, cDNA was synthesized using the iTaq Universal SYBR Green One-Step Kit (Bio-Rad) with specific primers (Table S1), 20 ng/reaction, and 300 nM primers in 10 μL of final reaction volume in a thermal cycler (Bio-Rad) with the following parameters: 50°C for 10 min, followed by 95°C for 1 min, and 39 cycles at 95°C for 10 s and 60°C for 30 s, followed by a melting curve from 65°C to 95°C.

    Red Blood Cell Lysis:

    Article Title: G-quadruplex-binding ligand RHPS4 reduces Plasmodium falciparum mitochondrial DNA level providing a novel antimalarial strategy.
    Article Snippet: .. To quantify the relative abundance of mRNA or rRNA by real-time quantitative polymerase chain reaction (qRT-PCR), total RNA was isolated from parasite pellets (after RBC lysis by saponin treatment) using the RNeasy Mini kit (Qiagen, Germantown, MD). .. Next, cDNA was synthesized using the iTaq Universal SYBR Green One-Step Kit (Bio-Rad) with specific primers (Table S1), 20 ng/reaction, and 300 nM primers in 10 μL of final reaction volume in a thermal cycler (Bio-Rad) with the following parameters: 50°C for 10 min, followed by 95°C for 1 min, and 39 cycles at 95°C for 10 s and 60°C for 30 s, followed by a melting curve from 65°C to 95°C.

    Reverse Transcription:

    Article Title: Variants in CEP135 Cause Congenital Microcephaly and Primary Ciliary Dyskinesia
    Article Snippet: .. Reverse transcription polymerase chain reaction (RT‐PCR) for CEP135 exons 6–9 was performed on RNA from HNECs isolated using a RNeasy Kit (Qiagen, Germantown, MD, USA) as per manufacturer's instructions. .. First strand cDNA was synthesized using SuperScript II Reverse Transcriptase kit (Thermo Fisher Scientific).

    Polymerase Chain Reaction:

    Article Title: Variants in CEP135 Cause Congenital Microcephaly and Primary Ciliary Dyskinesia
    Article Snippet: .. Reverse transcription polymerase chain reaction (RT‐PCR) for CEP135 exons 6–9 was performed on RNA from HNECs isolated using a RNeasy Kit (Qiagen, Germantown, MD, USA) as per manufacturer's instructions. .. First strand cDNA was synthesized using SuperScript II Reverse Transcriptase kit (Thermo Fisher Scientific).

    Concentration Assay:

    Article Title: Both Genetic Manipulation and Acupuncture Alleviate Depressive‐Like Behavior Mediated by Astrocytic P2X7 Receptors
    Article Snippet: .. Total RNA was extracted from the mPFC using an RNeasy Mini Kit (Qiagen, Venlo, The Netherlands), and a Nanodrop (Thermo Fisher Scientific, Waltham, MA, USA) was used to evaluate the concentration and quality (260/280 absorbance ratio) of the total RNA. .. For each reaction, 1 μg total RNA was added to a final volume of 20 μL of mixed reagent for reverse transcrition into cDNA using the QuantiNova Reverse Transcription Kit (Qiagen).

    Tissue:

    Article Title: Integrated morphological and transcriptomic analysis of bone disorders in aldosterone-salt hypertension.
    Article Snippet: .. Total RNA was isolated from femoral bone tissue and kidney cortical tissue using the RNeasy Mini Kit (Qiagen, Hilden, Germany). .. RNA quantity and quality were assessed using a NanoDrop-2000 spectrophotometer (Thermo Fisher Scientific) and a TapeStation system (Agilent Technologies, Santa Clara, CA, USA), respectively.



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    Image Search Results


    Experimental design of the study. ChIP-seq analysis was performed for open chromatin extracted from neuronal nuclei of post mortem PFC of multiple human subjects. Sample subsets from unaffected individuals and patients with psychiatric disorders were also used for WGS and RNA-seq analysis of total PFC tissue or white and gray matter of PFC. The arrows indicate the individual samples used in each analysis. FACS, fluorescence-activated cell sorting; FITC, fluorescein isothiocyanate; NeuN, neuronal nuclei.

    Journal: The FASEB Journal

    Article Title: Epigenetic-genetic chromatin footprinting identifies novel and subject-specific genes active in prefrontal cortex neurons

    doi: 10.1096/fj.201802646R

    Figure Lengend Snippet: Experimental design of the study. ChIP-seq analysis was performed for open chromatin extracted from neuronal nuclei of post mortem PFC of multiple human subjects. Sample subsets from unaffected individuals and patients with psychiatric disorders were also used for WGS and RNA-seq analysis of total PFC tissue or white and gray matter of PFC. The arrows indicate the individual samples used in each analysis. FACS, fluorescence-activated cell sorting; FITC, fluorescein isothiocyanate; NeuN, neuronal nuclei.

    Article Snippet: RNA (∼50 ng) was used to prepare indexed libraries with the ScriptSeq v.2 RNA Sequencing (RNA-Seq) Library Kit (Illumina).

    Techniques: ChIP-sequencing, RNA Sequencing Assay, Fluorescence, FACS

    Integration of H3K4me3 and RNA-seq data identified novel protein-coding and noncoding neuronal genes active in the human brain. A) Novel unannotated ncRNA gene with TSS mapped to the H3K4me3-marked region also harboring a promoter and TSS for the CCHC-type zinc finger nucleic acid binding protein gene. B) A novel ncRNA multiexonic gene with widely variable neuronal H3K4me3 abundance and expression in neurons from different individuals. C) Relative proportion of H3K4me3-marked promoter regions for previously annotated genes. D) Proportion of novel genes with notable protein-coding potential. Sample prefix indicates disease status; suffix indicates tissue for RNA-seq samples. H3K4me3 peaks are color-coded (red, schizophrenia; purple, autism; blue, control). A, autism; C, control; G, cortical gray matter; S, schizophrenia; W, cortical white matter; STG, superior temporal gyrus. For RNA-seq, blue lines show the connection of exons in spliced transcript reads.

    Journal: The FASEB Journal

    Article Title: Epigenetic-genetic chromatin footprinting identifies novel and subject-specific genes active in prefrontal cortex neurons

    doi: 10.1096/fj.201802646R

    Figure Lengend Snippet: Integration of H3K4me3 and RNA-seq data identified novel protein-coding and noncoding neuronal genes active in the human brain. A) Novel unannotated ncRNA gene with TSS mapped to the H3K4me3-marked region also harboring a promoter and TSS for the CCHC-type zinc finger nucleic acid binding protein gene. B) A novel ncRNA multiexonic gene with widely variable neuronal H3K4me3 abundance and expression in neurons from different individuals. C) Relative proportion of H3K4me3-marked promoter regions for previously annotated genes. D) Proportion of novel genes with notable protein-coding potential. Sample prefix indicates disease status; suffix indicates tissue for RNA-seq samples. H3K4me3 peaks are color-coded (red, schizophrenia; purple, autism; blue, control). A, autism; C, control; G, cortical gray matter; S, schizophrenia; W, cortical white matter; STG, superior temporal gyrus. For RNA-seq, blue lines show the connection of exons in spliced transcript reads.

    Article Snippet: RNA (∼50 ng) was used to prepare indexed libraries with the ScriptSeq v.2 RNA Sequencing (RNA-Seq) Library Kit (Illumina).

    Techniques: RNA Sequencing Assay, Binding Assay, Expressing

    Single nucleotide substitutions in the promoter are associated with induction of open chromatin and neuronal transcription activation of the testis-specific gene NUP21OL. A) Linkage of the rs114697636 SNP G allele, located near a start codon, and the rs11264875 SNP A allele, located at an exonic region of NUP210L. Monoallelic expression is demonstrated by direct Sanger sequencing of brain transcript cDNA from heterozygous carriers. B) Presence of the G allele at rs114697636 was strongly associated with an emerging H3K4me3 peak at the NUP210L promoter in all carriers. Asterisks denote the presence of the G allele in heterozygous (C28 and S14) and homozygous (A5) individuals. C) RT-PCR analysis of NUP210L relative to ubiquitin C (UBC) gene shows that NUP210L expression occurs in carriers of the rs114697636-G allele variant only (see also Supplemental Fig. S8). D) In general, NUP210L was characterized by testis-specific transcription and an active chromatin state of the promoter. ChIP-seq of testis (68) and RNA-seq of a panel of tissues from the Illumina BodyMap project are shown. A, autism; C, control; S, schizophrenia.

    Journal: The FASEB Journal

    Article Title: Epigenetic-genetic chromatin footprinting identifies novel and subject-specific genes active in prefrontal cortex neurons

    doi: 10.1096/fj.201802646R

    Figure Lengend Snippet: Single nucleotide substitutions in the promoter are associated with induction of open chromatin and neuronal transcription activation of the testis-specific gene NUP21OL. A) Linkage of the rs114697636 SNP G allele, located near a start codon, and the rs11264875 SNP A allele, located at an exonic region of NUP210L. Monoallelic expression is demonstrated by direct Sanger sequencing of brain transcript cDNA from heterozygous carriers. B) Presence of the G allele at rs114697636 was strongly associated with an emerging H3K4me3 peak at the NUP210L promoter in all carriers. Asterisks denote the presence of the G allele in heterozygous (C28 and S14) and homozygous (A5) individuals. C) RT-PCR analysis of NUP210L relative to ubiquitin C (UBC) gene shows that NUP210L expression occurs in carriers of the rs114697636-G allele variant only (see also Supplemental Fig. S8). D) In general, NUP210L was characterized by testis-specific transcription and an active chromatin state of the promoter. ChIP-seq of testis (68) and RNA-seq of a panel of tissues from the Illumina BodyMap project are shown. A, autism; C, control; S, schizophrenia.

    Article Snippet: RNA (∼50 ng) was used to prepare indexed libraries with the ScriptSeq v.2 RNA Sequencing (RNA-Seq) Library Kit (Illumina).

    Techniques: Activation Assay, Expressing, Sequencing, Reverse Transcription Polymerase Chain Reaction, Variant Assay, ChIP-sequencing, RNA Sequencing Assay