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one hybrid system  (TaKaRa)


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    Structured Review

    TaKaRa one hybrid system
    One Hybrid System, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 1235 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/screening+libraries/Matchmaker+Gold+Yeast+One-Hybrid+Library+Screening+System/pm42032297-337-9-11
    Average 98 stars, based on 1235 article reviews
    one hybrid system - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Library Screening:

    Article Title: FAD-Linked Oxidoreductase Protein 1 (FLO1) Coordinates Grain Development and Drought Tolerance in Rice.
    Article Snippet: Enzyme activities (SOD, POD, and CAT) and metabolite contents (proline and MDA) were determined using commercial assay kits (Solarbio, Beijing, China) according to the manufacturer’s instructions. .. The yeast one-hybrid system was performed according to Matchmaker Gold Yeast One-Hybrid Library Screening System User Manual (Clontech, Mountain View, CA, USA). ..

    Article Title: The transcriptional cascade CiERF023 <i>-</i> CiERF041 of <i>Citrus ichangenesis</i> regulates small heat shock protein gene <i>CiHSP26.5</i> to modulate cold tolerance
    Article Snippet: .. Screening of a 614 cDNA library was performed with the Matchmaker Gold Y1H Library Screening System (Cat. 615 No. 630491; Takara, Kyoto, Japan) with stringent selection with 40 ng/mL AbA (AbA; 616 Coolaber, Beijing, China). ..

    Article Title: FAD-Linked Oxidoreductase Protein 1 (FLO1) Coordinates Grain Development and Drought Tolerance in Rice
    Article Snippet: Enzyme activities (SOD, POD, and CAT) and metabolite contents (proline and MDA) were determined using commercial assay kits (Solarbio, Beijing, China) according to the manufacturer’s instructions. .. The yeast one-hybrid system was performed according to Matchmaker Gold Yeast One-Hybrid Library Screening System User Manual (Clontech, Mountain View, CA, USA). ..

    Article Title: Cotton GhLAGE3-mediated GhMML3 transcription activates fiber initiation upon reception of cell cycle exit signals
    Article Snippet: .. Y1H screening was conducted following the manufacturer’s instructions of the Matchmaker Gold Yeast One-Hybrid Library Screening System (Clontech, America). ..

    cDNA Library Assay:

    Article Title: The transcriptional cascade CiERF023 <i>-</i> CiERF041 of <i>Citrus ichangenesis</i> regulates small heat shock protein gene <i>CiHSP26.5</i> to modulate cold tolerance
    Article Snippet: .. Screening of a 614 cDNA library was performed with the Matchmaker Gold Y1H Library Screening System (Cat. 615 No. 630491; Takara, Kyoto, Japan) with stringent selection with 40 ng/mL AbA (AbA; 616 Coolaber, Beijing, China). ..

    Selection:

    Article Title: The transcriptional cascade CiERF023 <i>-</i> CiERF041 of <i>Citrus ichangenesis</i> regulates small heat shock protein gene <i>CiHSP26.5</i> to modulate cold tolerance
    Article Snippet: .. Screening of a 614 cDNA library was performed with the Matchmaker Gold Y1H Library Screening System (Cat. 615 No. 630491; Takara, Kyoto, Japan) with stringent selection with 40 ng/mL AbA (AbA; 616 Coolaber, Beijing, China). ..

    Clone Assay:

    Article Title: Somatic salt stress memory enhances tolerance in poplar: Insights from integrated transcriptome and physiological analyses
    Article Snippet: .. The coding sequences of the selected transcription factors and the promoter sequences of the target HAK genes were cloned into the activation domain vector (pGADT7, Clontech, PT3249–5) and the bait vector (pAbAi, TAKARA, 630491), respectively. ..

    Activation Assay:

    Article Title: Somatic salt stress memory enhances tolerance in poplar: Insights from integrated transcriptome and physiological analyses
    Article Snippet: .. The coding sequences of the selected transcription factors and the promoter sequences of the target HAK genes were cloned into the activation domain vector (pGADT7, Clontech, PT3249–5) and the bait vector (pAbAi, TAKARA, 630491), respectively. ..

    Plasmid Preparation:

    Article Title: Somatic salt stress memory enhances tolerance in poplar: Insights from integrated transcriptome and physiological analyses
    Article Snippet: .. The coding sequences of the selected transcription factors and the promoter sequences of the target HAK genes were cloned into the activation domain vector (pGADT7, Clontech, PT3249–5) and the bait vector (pAbAi, TAKARA, 630491), respectively. ..



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