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nexterion h amine-reactive, n-hydroxysuccinimide ester functionalized, hydrogel-coated glass slides  (SCHOTT)

 
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    Structured Review

    SCHOTT nexterion h amine-reactive, n-hydroxysuccinimide ester functionalized, hydrogel-coated glass slides
    Nexterion H Amine Reactive, N Hydroxysuccinimide Ester Functionalized, Hydrogel Coated Glass Slides, supplied by SCHOTT, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sciflexarrayer+s3+noncontact+microarray+printer/nexterion+h+nhs+activated+hydrogel+microarray+glass+slides/pmc07551182-70-45-42
    Average 90 stars, based on 1 article reviews
    nexterion h amine-reactive, n-hydroxysuccinimide ester functionalized, hydrogel-coated glass slides - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: Nanovibrational Stimulation of Mesenchymal Stem Cells Induces Therapeutic Reactive Oxygen Species and Inflammation for Three-Dimensional Bone Tissue Engineering
    Article Snippet: Antibodies were diluted to print concentration in PBS and printed in six replicates on Nexterion H amine reactive hydrogel-coated glass slides (Schott AG, Mainz, Germany) using a SciFLEXARRAYER S3 piezoelectric printer (Scienion, Berlin, Germany) under constant humidity (62% ± 2%) at 20 °C.

    Article Title: Structural insights into a cooperative switch between one and two FimH bacterial adhesins binding pauci- and high-mannose type N -glycan receptors
    Article Snippet: Briefly, 50 μM ligand solutions (1.25 nl, 5 drops, 250 pL drop volume) in sodium phosphate buffer (300 mM, 0.005% Tween-20, pH = 8.4) were spatially arrayed employing a robotic noncontact piezoelectric spotter (SciFLEXARRAYER S11, Scienion) onto N -hydroxysuccinimide (NHS) activated glass slides (Nexterion H, Schott AG).

    Article Title: Identification of lectin receptors for conserved SARS‐CoV‐2 glycosylation sites
    Article Snippet: Briefly, 50 μM ligand solutions (1.25 nl, 5 drops, 250 pl drop volume) in sodium phosphate buffer (300 mM, 0.005% Tween‐20, pH = 8.4) were spatially arrayed employing a robotic non‐contact piezoelectric spotter (SciFLEXARRAYER S11, Scienion) onto N‐hydroxysuccinimide (NHS) activated glass slides (Nexterion H, Schott AG).

    Concentration Assay:

    Article Title: Nanovibrational stimulation of mesenchymal stem cells induces therapeutic reactive oxygen species and inflammation for 3D bone tissue engineering
    Article Snippet: .. Antibodies were diluted to print concentration in PBS and printed in six replicates on Nexterion H amine reactive hydrogel coated glass slides (Schott AG, Mainz, Germany) using a SciFLEXARRAYER S3 piezoelectric printer (Scienion, Berlin, Germany) under constant humidity (62% +/− 2%) at 20 °C. ..

    Microarray:

    Article Title: l ‐DNA Duplex Formation as a Bioorthogonal Information Channel in Nucleic Acid‐Based Surface Patterning
    Article Snippet: .. Microarray synthesis was performed on functionalized Schott NEXTERION glass D slides. ..

    Article Title: Effects of Exogenous Dietary Advanced Glycation End Products on the Cross-Talk Mechanisms Linking Microbiota to Metabolic Inflammation
    Article Snippet: Both fractions, IgGs and IgG-depleted blood, were buffered exchanged with PBS and directly fluorescently labelled with Alexa Fluor ® 555 (Biosciences, ThermoFisher, Dublin, Ireland) following manufacturer’s instructions, in the dark. .. Lectin microarray were prepared by dilution of lectins of known specificities in phosphate buffered saline (PBS), pH 7.4, containing 1 mM concentrations of their respective haptenic sugar to ensure preservation of their binding site ( ) and printed on Nexterion ® H (Schott, Mainz, Germany) amine-reactive, N-hydroxysuccinimide ester functionalized, hydrogel-coated glass slides using a SciFlexArrayer S3 piezoelectric spotter (Scienion, Berlin, Germany) under constant 62% (+/−2%) humidity at 20 °C. ..

    Saline:

    Article Title: Effects of Exogenous Dietary Advanced Glycation End Products on the Cross-Talk Mechanisms Linking Microbiota to Metabolic Inflammation
    Article Snippet: Both fractions, IgGs and IgG-depleted blood, were buffered exchanged with PBS and directly fluorescently labelled with Alexa Fluor ® 555 (Biosciences, ThermoFisher, Dublin, Ireland) following manufacturer’s instructions, in the dark. .. Lectin microarray were prepared by dilution of lectins of known specificities in phosphate buffered saline (PBS), pH 7.4, containing 1 mM concentrations of their respective haptenic sugar to ensure preservation of their binding site ( ) and printed on Nexterion ® H (Schott, Mainz, Germany) amine-reactive, N-hydroxysuccinimide ester functionalized, hydrogel-coated glass slides using a SciFlexArrayer S3 piezoelectric spotter (Scienion, Berlin, Germany) under constant 62% (+/−2%) humidity at 20 °C. ..

    Preserving:

    Article Title: Effects of Exogenous Dietary Advanced Glycation End Products on the Cross-Talk Mechanisms Linking Microbiota to Metabolic Inflammation
    Article Snippet: Both fractions, IgGs and IgG-depleted blood, were buffered exchanged with PBS and directly fluorescently labelled with Alexa Fluor ® 555 (Biosciences, ThermoFisher, Dublin, Ireland) following manufacturer’s instructions, in the dark. .. Lectin microarray were prepared by dilution of lectins of known specificities in phosphate buffered saline (PBS), pH 7.4, containing 1 mM concentrations of their respective haptenic sugar to ensure preservation of their binding site ( ) and printed on Nexterion ® H (Schott, Mainz, Germany) amine-reactive, N-hydroxysuccinimide ester functionalized, hydrogel-coated glass slides using a SciFlexArrayer S3 piezoelectric spotter (Scienion, Berlin, Germany) under constant 62% (+/−2%) humidity at 20 °C. ..

    Binding Assay:

    Article Title: Effects of Exogenous Dietary Advanced Glycation End Products on the Cross-Talk Mechanisms Linking Microbiota to Metabolic Inflammation
    Article Snippet: Both fractions, IgGs and IgG-depleted blood, were buffered exchanged with PBS and directly fluorescently labelled with Alexa Fluor ® 555 (Biosciences, ThermoFisher, Dublin, Ireland) following manufacturer’s instructions, in the dark. .. Lectin microarray were prepared by dilution of lectins of known specificities in phosphate buffered saline (PBS), pH 7.4, containing 1 mM concentrations of their respective haptenic sugar to ensure preservation of their binding site ( ) and printed on Nexterion ® H (Schott, Mainz, Germany) amine-reactive, N-hydroxysuccinimide ester functionalized, hydrogel-coated glass slides using a SciFlexArrayer S3 piezoelectric spotter (Scienion, Berlin, Germany) under constant 62% (+/−2%) humidity at 20 °C. ..



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    SCIENION noncontact microarray printer
    <t>Microarray</t> analysis reveals the effects of GFAP mAb treatment on key inflammatory mediators in glaucomatous retina. ( A - C ) The expression of TLR4 and S100A8 was significantly downregulated by 25–50 µg GFAP mAb treatment compared to the vehicle. The marker for microglial activation, CD68, was significantly decreased in the 25 µg GFAP mAb treatment group. ( D - F ) Proteins of the inflammasome pathway, NLRP3, GSDMD and Caspase-1 were significantly downregulated in the 25 µg GFAP mAb treatment group. With the 50 µg dose, these protein expressions showed a similar downward trend, but without statistical significance. ( G ) Expression profiling of inflammation-associated mediators showed that 25 µg GFAP mAb significantly decreased pro-inflammatory factors (TNF-α, IL-1β, IL-8, MMP9, and IFN-γ), and increased the anti-inflammatory cytokine IL-10 compared to the vehicle group. In contrast, the 50 µg dose significantly reduced IFN-γ only. ( H ) Representative images of spots showing IL-1β levels in the subarrays for each group. Image analysis was conducted with Imagene software, using the median intensity from each spot to calculate the mean of the triplicate spots for each marker. For CD68 and TLR4, due to significant heterogeneity in SDs, statistical analysis was performed using Welch’s ANOVA followed by the Tamhane T2 post hoc test. All other data were analyzed using one-way ANOVA with Tukey’s post hoc test. Data are presented as the mean ± SD; n = 4 per group; * p < 0.05, ** p < 0.01, *** p < 0.001; ns = not significant. TLR4 – Toll-Like Receptor 4. S100A8 – S100 Calcium Binding Protein A8. NLRP3 – NLR Family Pyrin Domain Containing 3. GSDMD – Gasdermin D
    Noncontact Microarray Printer, supplied by SCIENION, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sciflexarrayer+s3+noncontact+microarray+printer/sciFLEXARRAYER+S3/pmc12175471-139-7-12
    Average 96 stars, based on 1 article reviews
    noncontact microarray printer - by Bioz Stars, 2026-10
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    96
    SCIENION sciflexarrayer s3 noncontact microarray printer
    <t>Microarray</t> analysis reveals the effects of GFAP mAb treatment on key inflammatory mediators in glaucomatous retina. ( A - C ) The expression of TLR4 and S100A8 was significantly downregulated by 25–50 µg GFAP mAb treatment compared to the vehicle. The marker for microglial activation, CD68, was significantly decreased in the 25 µg GFAP mAb treatment group. ( D - F ) Proteins of the inflammasome pathway, NLRP3, GSDMD and Caspase-1 were significantly downregulated in the 25 µg GFAP mAb treatment group. With the 50 µg dose, these protein expressions showed a similar downward trend, but without statistical significance. ( G ) Expression profiling of inflammation-associated mediators showed that 25 µg GFAP mAb significantly decreased pro-inflammatory factors (TNF-α, IL-1β, IL-8, MMP9, and IFN-γ), and increased the anti-inflammatory cytokine IL-10 compared to the vehicle group. In contrast, the 50 µg dose significantly reduced IFN-γ only. ( H ) Representative images of spots showing IL-1β levels in the subarrays for each group. Image analysis was conducted with Imagene software, using the median intensity from each spot to calculate the mean of the triplicate spots for each marker. For CD68 and TLR4, due to significant heterogeneity in SDs, statistical analysis was performed using Welch’s ANOVA followed by the Tamhane T2 post hoc test. All other data were analyzed using one-way ANOVA with Tukey’s post hoc test. Data are presented as the mean ± SD; n = 4 per group; * p < 0.05, ** p < 0.01, *** p < 0.001; ns = not significant. TLR4 – Toll-Like Receptor 4. S100A8 – S100 Calcium Binding Protein A8. NLRP3 – NLR Family Pyrin Domain Containing 3. GSDMD – Gasdermin D
    Sciflexarrayer S3 Noncontact Microarray Printer, supplied by SCIENION, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sciflexarrayer+s3+noncontact+microarray+printer/sciFLEXARRAYER+S3/pm35750689-225-14-13
    Average 96 stars, based on 1 article reviews
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    Microarray analysis reveals the effects of GFAP mAb treatment on key inflammatory mediators in glaucomatous retina. ( A - C ) The expression of TLR4 and S100A8 was significantly downregulated by 25–50 µg GFAP mAb treatment compared to the vehicle. The marker for microglial activation, CD68, was significantly decreased in the 25 µg GFAP mAb treatment group. ( D - F ) Proteins of the inflammasome pathway, NLRP3, GSDMD and Caspase-1 were significantly downregulated in the 25 µg GFAP mAb treatment group. With the 50 µg dose, these protein expressions showed a similar downward trend, but without statistical significance. ( G ) Expression profiling of inflammation-associated mediators showed that 25 µg GFAP mAb significantly decreased pro-inflammatory factors (TNF-α, IL-1β, IL-8, MMP9, and IFN-γ), and increased the anti-inflammatory cytokine IL-10 compared to the vehicle group. In contrast, the 50 µg dose significantly reduced IFN-γ only. ( H ) Representative images of spots showing IL-1β levels in the subarrays for each group. Image analysis was conducted with Imagene software, using the median intensity from each spot to calculate the mean of the triplicate spots for each marker. For CD68 and TLR4, due to significant heterogeneity in SDs, statistical analysis was performed using Welch’s ANOVA followed by the Tamhane T2 post hoc test. All other data were analyzed using one-way ANOVA with Tukey’s post hoc test. Data are presented as the mean ± SD; n = 4 per group; * p < 0.05, ** p < 0.01, *** p < 0.001; ns = not significant. TLR4 – Toll-Like Receptor 4. S100A8 – S100 Calcium Binding Protein A8. NLRP3 – NLR Family Pyrin Domain Containing 3. GSDMD – Gasdermin D

    Journal: Journal of Neuroinflammation

    Article Title: Targeting glial fibrillary acidic protein in glaucoma: a monoclonal antibody approach to modulate glial reactivity and neuroinflammation for neuroprotection

    doi: 10.1186/s12974-025-03482-8

    Figure Lengend Snippet: Microarray analysis reveals the effects of GFAP mAb treatment on key inflammatory mediators in glaucomatous retina. ( A - C ) The expression of TLR4 and S100A8 was significantly downregulated by 25–50 µg GFAP mAb treatment compared to the vehicle. The marker for microglial activation, CD68, was significantly decreased in the 25 µg GFAP mAb treatment group. ( D - F ) Proteins of the inflammasome pathway, NLRP3, GSDMD and Caspase-1 were significantly downregulated in the 25 µg GFAP mAb treatment group. With the 50 µg dose, these protein expressions showed a similar downward trend, but without statistical significance. ( G ) Expression profiling of inflammation-associated mediators showed that 25 µg GFAP mAb significantly decreased pro-inflammatory factors (TNF-α, IL-1β, IL-8, MMP9, and IFN-γ), and increased the anti-inflammatory cytokine IL-10 compared to the vehicle group. In contrast, the 50 µg dose significantly reduced IFN-γ only. ( H ) Representative images of spots showing IL-1β levels in the subarrays for each group. Image analysis was conducted with Imagene software, using the median intensity from each spot to calculate the mean of the triplicate spots for each marker. For CD68 and TLR4, due to significant heterogeneity in SDs, statistical analysis was performed using Welch’s ANOVA followed by the Tamhane T2 post hoc test. All other data were analyzed using one-way ANOVA with Tukey’s post hoc test. Data are presented as the mean ± SD; n = 4 per group; * p < 0.05, ** p < 0.01, *** p < 0.001; ns = not significant. TLR4 – Toll-Like Receptor 4. S100A8 – S100 Calcium Binding Protein A8. NLRP3 – NLR Family Pyrin Domain Containing 3. GSDMD – Gasdermin D

    Article Snippet: Briefly, the microarray was prepared using a noncontact microarray printer (SciFLEXARRAYER S3; Scienion, Berlin, Germany).

    Techniques: Microarray, Expressing, Marker, Activation Assay, Software, Binding Assay