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c18 ziptip  (Merck KGaA)

 
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    Structured Review

    Merck KGaA c18 ziptip
    C18 Ziptip, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sciflexarrayer+s3+noncontact+microarray+printer/c18+ziptips/pm37209096-772-97-94
    Average 90 stars, based on 1 article reviews
    c18 ziptip - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Immunopeptidomics:

    Article Title: Methods and compositions for use of tumor self-antigens in adoptive immunotherapy
    Article Snippet: Elution fractions E1-E4 were pooled and free MHC ligands were isolated by ultrafiltration using centrifugal filter units (Amicon; Merck Millipore). .. MHC ligands were extracted and desalted from the filtrate using ZipTip C18 pipette tips (Merck Millipore). .. Extracted peptides were eluted in 35 μl of acetonitrile (Merck)/0.1% trifluoroacetic acid, centrifuged to complete dryness and resuspended in 25 μl of 1% acetonitrile/0.05% trifluoroacetic acid.

    Transferring:

    Article Title: Methods and compositions for use of tumor self-antigens in adoptive immunotherapy
    Article Snippet: Elution fractions E1-E4 were pooled and free MHC ligands were isolated by ultrafiltration using centrifugal filter units (Amicon; Merck Millipore). .. MHC ligands were extracted and desalted from the filtrate using ZipTip C18 pipette tips (Merck Millipore). .. Extracted peptides were eluted in 35 μl of acetonitrile (Merck)/0.1% trifluoroacetic acid, centrifuged to complete dryness and resuspended in 25 μl of 1% acetonitrile/0.05% trifluoroacetic acid.

    Mass Spectrometry:

    Article Title: Isolation of Dehalococcoides mccartyi strain (NIT-OBY) and identification of a reductive dehalogenase dechlorinating cis-1,3-dichloropropene but not trans-1,3-dichloropropene to non-toxic propene.
    Article Snippet: .. Extracted peptide samples were desalted using C18 Zip Tip columns (Merck Millipore, Darmstadt, Germany) prior to analysis via nLC-MS/MS using an Orbitrap Fusion Tribrid mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) equipped with a nano-UPLC system (Thermo Fisher Scientific). .. Peptide identification was conducted using Proteome Discoverer (v2.2, Thermo Fisher Scientific) with SequestHT as the search engine and a FASTA file of proteins from the genome of strain NIT-OBY [45].

    other:

    Article Title: [ 68 Ga]Ga-interleukin-2 for imaging activated T-lymphocytes: biochemical characterization and phase I study in normal subjects.
    Article Snippet: The obtained peptide blends were purified using a C18 zip-tip (Merk Life Science S.r.l., Milano, Italy), dried and re-suspended in water containing 0.2% trifluoroacetic acid.

    Article Title: Metaproteomic Dataset on Semi-Diurnal Variability of the Bacterioplankton Communities During a Spring Phytoplankton Bloom in the North Sea.
    Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense ZipTip C18 (Merck Millipore, P10 tip size, Burlington, MA, USA) according to the manufacturer’s instructions and loaded onto in-house packed capillary columns (20 cm length, 75 μm inner diameter, Dr. Maisch GmbH, Ammerbruch, Germany, RepsoSil pur C18 material with pore size 120 Å and particle size 1.9 μm) via an Easy nLC1000 LC (Thermo Fisher Scientific, Waltham, MA, USA) coupled to a Q Exactive mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) in data-dependent acquisition mode and separated using a non-linear binary gradient (131 min) from 1% to 99% solvent B (99.9% ACN [v/v], 0.1% acetic acid [v/v]) in solvent A at a constant flow rate of 300 nL/min.

    Article Title: Proteomic profiling of 15-day post-mating preimplantation alpaca embryos reveals estradiol and cortisol pathway signatures.
    Article Snippet: South American camelids (SACs) play an important role in Andean economies, but reproductive inefficiencies, especially early embryonic losses, present significant challenges to herd productivity.. This study provides the first proteomic analysis of preimplantation alpaca embryos, offering insights into the molecular mechanisms underlying embryo development and implantation.. Using mass spectrometry, we identified 510 proteins in 15day post-mating (dpm) alpaca embryos.

    Solvent:

    Article Title: A metabolic inhibitor blocks cellular fucosylation and enables production of afucosylated antibodies
    Article Snippet: .. The digested peptides were cleaned up using ZipTip C18 (Merck Millipore), dried down, and redissolved in 0.1 % formic acid (solvent A). .. Data were acquired on an Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific) fitted with an Easy-nLC 1200 (Thermo Fisher Scientific).



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    <t>Microarray</t> analysis reveals the effects of GFAP mAb treatment on key inflammatory mediators in glaucomatous retina. ( A - C ) The expression of TLR4 and S100A8 was significantly downregulated by 25–50 µg GFAP mAb treatment compared to the vehicle. The marker for microglial activation, CD68, was significantly decreased in the 25 µg GFAP mAb treatment group. ( D - F ) Proteins of the inflammasome pathway, NLRP3, GSDMD and Caspase-1 were significantly downregulated in the 25 µg GFAP mAb treatment group. With the 50 µg dose, these protein expressions showed a similar downward trend, but without statistical significance. ( G ) Expression profiling of inflammation-associated mediators showed that 25 µg GFAP mAb significantly decreased pro-inflammatory factors (TNF-α, IL-1β, IL-8, MMP9, and IFN-γ), and increased the anti-inflammatory cytokine IL-10 compared to the vehicle group. In contrast, the 50 µg dose significantly reduced IFN-γ only. ( H ) Representative images of spots showing IL-1β levels in the subarrays for each group. Image analysis was conducted with Imagene software, using the median intensity from each spot to calculate the mean of the triplicate spots for each marker. For CD68 and TLR4, due to significant heterogeneity in SDs, statistical analysis was performed using Welch’s ANOVA followed by the Tamhane T2 post hoc test. All other data were analyzed using one-way ANOVA with Tukey’s post hoc test. Data are presented as the mean ± SD; n = 4 per group; * p < 0.05, ** p < 0.01, *** p < 0.001; ns = not significant. TLR4 – Toll-Like Receptor 4. S100A8 – S100 Calcium Binding Protein A8. NLRP3 – NLR Family Pyrin Domain Containing 3. GSDMD – Gasdermin D
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    Image Search Results


    Microarray analysis reveals the effects of GFAP mAb treatment on key inflammatory mediators in glaucomatous retina. ( A - C ) The expression of TLR4 and S100A8 was significantly downregulated by 25–50 µg GFAP mAb treatment compared to the vehicle. The marker for microglial activation, CD68, was significantly decreased in the 25 µg GFAP mAb treatment group. ( D - F ) Proteins of the inflammasome pathway, NLRP3, GSDMD and Caspase-1 were significantly downregulated in the 25 µg GFAP mAb treatment group. With the 50 µg dose, these protein expressions showed a similar downward trend, but without statistical significance. ( G ) Expression profiling of inflammation-associated mediators showed that 25 µg GFAP mAb significantly decreased pro-inflammatory factors (TNF-α, IL-1β, IL-8, MMP9, and IFN-γ), and increased the anti-inflammatory cytokine IL-10 compared to the vehicle group. In contrast, the 50 µg dose significantly reduced IFN-γ only. ( H ) Representative images of spots showing IL-1β levels in the subarrays for each group. Image analysis was conducted with Imagene software, using the median intensity from each spot to calculate the mean of the triplicate spots for each marker. For CD68 and TLR4, due to significant heterogeneity in SDs, statistical analysis was performed using Welch’s ANOVA followed by the Tamhane T2 post hoc test. All other data were analyzed using one-way ANOVA with Tukey’s post hoc test. Data are presented as the mean ± SD; n = 4 per group; * p < 0.05, ** p < 0.01, *** p < 0.001; ns = not significant. TLR4 – Toll-Like Receptor 4. S100A8 – S100 Calcium Binding Protein A8. NLRP3 – NLR Family Pyrin Domain Containing 3. GSDMD – Gasdermin D

    Journal: Journal of Neuroinflammation

    Article Title: Targeting glial fibrillary acidic protein in glaucoma: a monoclonal antibody approach to modulate glial reactivity and neuroinflammation for neuroprotection

    doi: 10.1186/s12974-025-03482-8

    Figure Lengend Snippet: Microarray analysis reveals the effects of GFAP mAb treatment on key inflammatory mediators in glaucomatous retina. ( A - C ) The expression of TLR4 and S100A8 was significantly downregulated by 25–50 µg GFAP mAb treatment compared to the vehicle. The marker for microglial activation, CD68, was significantly decreased in the 25 µg GFAP mAb treatment group. ( D - F ) Proteins of the inflammasome pathway, NLRP3, GSDMD and Caspase-1 were significantly downregulated in the 25 µg GFAP mAb treatment group. With the 50 µg dose, these protein expressions showed a similar downward trend, but without statistical significance. ( G ) Expression profiling of inflammation-associated mediators showed that 25 µg GFAP mAb significantly decreased pro-inflammatory factors (TNF-α, IL-1β, IL-8, MMP9, and IFN-γ), and increased the anti-inflammatory cytokine IL-10 compared to the vehicle group. In contrast, the 50 µg dose significantly reduced IFN-γ only. ( H ) Representative images of spots showing IL-1β levels in the subarrays for each group. Image analysis was conducted with Imagene software, using the median intensity from each spot to calculate the mean of the triplicate spots for each marker. For CD68 and TLR4, due to significant heterogeneity in SDs, statistical analysis was performed using Welch’s ANOVA followed by the Tamhane T2 post hoc test. All other data were analyzed using one-way ANOVA with Tukey’s post hoc test. Data are presented as the mean ± SD; n = 4 per group; * p < 0.05, ** p < 0.01, *** p < 0.001; ns = not significant. TLR4 – Toll-Like Receptor 4. S100A8 – S100 Calcium Binding Protein A8. NLRP3 – NLR Family Pyrin Domain Containing 3. GSDMD – Gasdermin D

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    Techniques: Microarray, Expressing, Marker, Activation Assay, Software, Binding Assay