Review




Structured Review

BioDot Inc non contact microarrayer sciflexarrayer

Non Contact Microarrayer Sciflexarrayer, supplied by BioDot Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sciflexarrayer+s3+non-contact+microarray/non+contact+microarrayer+sciflexarrayer/pmc03466934-51-7-4
Average 90 stars, based on 1 article reviews
non contact microarrayer sciflexarrayer - by Bioz Stars, 2026-11
90/100 stars

Images

1) Product Images from "Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples"

Article Title: Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Journal: Journal of Visualized Experiments : JoVE

doi: 10.3791/3791


Figure Legend Snippet:

Techniques Used: Microarray, Staining, Incubation, Saline, Protease Inhibitor

Related Articles

Microarray:

Article Title: Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
Article Snippet: Name of the reagent s/equipments Company Catalogue number Non contact microarrayer BioDot Inc sciFLEXARRAYER 384 microplate Fisher 14-230-243 FoodSaver FoodSaver V3835 Ultrathin nitrocellulose coate microarray slides Gentel PATH Slide Imprinter (optional) The Gel Company WSP60-1 Shaker Fisher 15-453-211 Centrifuge Eppendorf 5804 000.013 Slide washing basin/Slide Staining Dish with Removable Rack Fisher 08-812 Slide incubation chamber/microscope slide box Fisher 03-448-5 Brij 35, 30 w/v% solution in water Acros Organics AC32958-0025 Tween-20 Fisher P337-100 Sodium Periodate (NaIO 4 ) Sigma 311448 L-Glutamic acid γ-hydrazide Sigma G-7257 Sodium Acetate Anhydrous (CH 3 COONa) Sigma S2889 Bovine Serum Albumin (BSA) Lampire Biological Labs 7500804 Phosphate Buffer Saline (PBS) (10X) Denville Scientific CP4390-48 Dylight 549 conjugated NeutrAvidin Thermo 22837 Protease Inhibitor Cocktail Tablets Roche 4693159001 ChromPure Human IgG, Fc fragment Jackson Immunoresearch 009-000-008 ChromPure Human IgG, whole molecule Jackson Immunoresearch 009-000-003 ChromPure Mouse IgG, whole molecule Jackson Immunoresearch 015-000-003 ChromPure Mouse IgG, Fc fragment Jackson Immunoresearch 015-000-008 ChromPure Rabbit IgG, whole molecule Jackson Immunoresearch 011-000-003 ChromPure Donkey IgG, whole molecule Jackson Immunoresearch 017-000-003 Microarray Scanner Tecan LS Reloaded Open in a separate window Table 2.

Staining:

Article Title: Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
Article Snippet: Name of the reagent s/equipments Company Catalogue number Non contact microarrayer BioDot Inc sciFLEXARRAYER 384 microplate Fisher 14-230-243 FoodSaver FoodSaver V3835 Ultrathin nitrocellulose coate microarray slides Gentel PATH Slide Imprinter (optional) The Gel Company WSP60-1 Shaker Fisher 15-453-211 Centrifuge Eppendorf 5804 000.013 Slide washing basin/Slide Staining Dish with Removable Rack Fisher 08-812 Slide incubation chamber/microscope slide box Fisher 03-448-5 Brij 35, 30 w/v% solution in water Acros Organics AC32958-0025 Tween-20 Fisher P337-100 Sodium Periodate (NaIO 4 ) Sigma 311448 L-Glutamic acid γ-hydrazide Sigma G-7257 Sodium Acetate Anhydrous (CH 3 COONa) Sigma S2889 Bovine Serum Albumin (BSA) Lampire Biological Labs 7500804 Phosphate Buffer Saline (PBS) (10X) Denville Scientific CP4390-48 Dylight 549 conjugated NeutrAvidin Thermo 22837 Protease Inhibitor Cocktail Tablets Roche 4693159001 ChromPure Human IgG, Fc fragment Jackson Immunoresearch 009-000-008 ChromPure Human IgG, whole molecule Jackson Immunoresearch 009-000-003 ChromPure Mouse IgG, whole molecule Jackson Immunoresearch 015-000-003 ChromPure Mouse IgG, Fc fragment Jackson Immunoresearch 015-000-008 ChromPure Rabbit IgG, whole molecule Jackson Immunoresearch 011-000-003 ChromPure Donkey IgG, whole molecule Jackson Immunoresearch 017-000-003 Microarray Scanner Tecan LS Reloaded Open in a separate window Table 2.

Incubation:

Article Title: Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
Article Snippet: Name of the reagent s/equipments Company Catalogue number Non contact microarrayer BioDot Inc sciFLEXARRAYER 384 microplate Fisher 14-230-243 FoodSaver FoodSaver V3835 Ultrathin nitrocellulose coate microarray slides Gentel PATH Slide Imprinter (optional) The Gel Company WSP60-1 Shaker Fisher 15-453-211 Centrifuge Eppendorf 5804 000.013 Slide washing basin/Slide Staining Dish with Removable Rack Fisher 08-812 Slide incubation chamber/microscope slide box Fisher 03-448-5 Brij 35, 30 w/v% solution in water Acros Organics AC32958-0025 Tween-20 Fisher P337-100 Sodium Periodate (NaIO 4 ) Sigma 311448 L-Glutamic acid γ-hydrazide Sigma G-7257 Sodium Acetate Anhydrous (CH 3 COONa) Sigma S2889 Bovine Serum Albumin (BSA) Lampire Biological Labs 7500804 Phosphate Buffer Saline (PBS) (10X) Denville Scientific CP4390-48 Dylight 549 conjugated NeutrAvidin Thermo 22837 Protease Inhibitor Cocktail Tablets Roche 4693159001 ChromPure Human IgG, Fc fragment Jackson Immunoresearch 009-000-008 ChromPure Human IgG, whole molecule Jackson Immunoresearch 009-000-003 ChromPure Mouse IgG, whole molecule Jackson Immunoresearch 015-000-003 ChromPure Mouse IgG, Fc fragment Jackson Immunoresearch 015-000-008 ChromPure Rabbit IgG, whole molecule Jackson Immunoresearch 011-000-003 ChromPure Donkey IgG, whole molecule Jackson Immunoresearch 017-000-003 Microarray Scanner Tecan LS Reloaded Open in a separate window Table 2.

Saline:

Article Title: Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
Article Snippet: Name of the reagent s/equipments Company Catalogue number Non contact microarrayer BioDot Inc sciFLEXARRAYER 384 microplate Fisher 14-230-243 FoodSaver FoodSaver V3835 Ultrathin nitrocellulose coate microarray slides Gentel PATH Slide Imprinter (optional) The Gel Company WSP60-1 Shaker Fisher 15-453-211 Centrifuge Eppendorf 5804 000.013 Slide washing basin/Slide Staining Dish with Removable Rack Fisher 08-812 Slide incubation chamber/microscope slide box Fisher 03-448-5 Brij 35, 30 w/v% solution in water Acros Organics AC32958-0025 Tween-20 Fisher P337-100 Sodium Periodate (NaIO 4 ) Sigma 311448 L-Glutamic acid γ-hydrazide Sigma G-7257 Sodium Acetate Anhydrous (CH 3 COONa) Sigma S2889 Bovine Serum Albumin (BSA) Lampire Biological Labs 7500804 Phosphate Buffer Saline (PBS) (10X) Denville Scientific CP4390-48 Dylight 549 conjugated NeutrAvidin Thermo 22837 Protease Inhibitor Cocktail Tablets Roche 4693159001 ChromPure Human IgG, Fc fragment Jackson Immunoresearch 009-000-008 ChromPure Human IgG, whole molecule Jackson Immunoresearch 009-000-003 ChromPure Mouse IgG, whole molecule Jackson Immunoresearch 015-000-003 ChromPure Mouse IgG, Fc fragment Jackson Immunoresearch 015-000-008 ChromPure Rabbit IgG, whole molecule Jackson Immunoresearch 011-000-003 ChromPure Donkey IgG, whole molecule Jackson Immunoresearch 017-000-003 Microarray Scanner Tecan LS Reloaded Open in a separate window Table 2.

Protease Inhibitor:

Article Title: Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
Article Snippet: Name of the reagent s/equipments Company Catalogue number Non contact microarrayer BioDot Inc sciFLEXARRAYER 384 microplate Fisher 14-230-243 FoodSaver FoodSaver V3835 Ultrathin nitrocellulose coate microarray slides Gentel PATH Slide Imprinter (optional) The Gel Company WSP60-1 Shaker Fisher 15-453-211 Centrifuge Eppendorf 5804 000.013 Slide washing basin/Slide Staining Dish with Removable Rack Fisher 08-812 Slide incubation chamber/microscope slide box Fisher 03-448-5 Brij 35, 30 w/v% solution in water Acros Organics AC32958-0025 Tween-20 Fisher P337-100 Sodium Periodate (NaIO 4 ) Sigma 311448 L-Glutamic acid γ-hydrazide Sigma G-7257 Sodium Acetate Anhydrous (CH 3 COONa) Sigma S2889 Bovine Serum Albumin (BSA) Lampire Biological Labs 7500804 Phosphate Buffer Saline (PBS) (10X) Denville Scientific CP4390-48 Dylight 549 conjugated NeutrAvidin Thermo 22837 Protease Inhibitor Cocktail Tablets Roche 4693159001 ChromPure Human IgG, Fc fragment Jackson Immunoresearch 009-000-008 ChromPure Human IgG, whole molecule Jackson Immunoresearch 009-000-003 ChromPure Mouse IgG, whole molecule Jackson Immunoresearch 015-000-003 ChromPure Mouse IgG, Fc fragment Jackson Immunoresearch 015-000-008 ChromPure Rabbit IgG, whole molecule Jackson Immunoresearch 011-000-003 ChromPure Donkey IgG, whole molecule Jackson Immunoresearch 017-000-003 Microarray Scanner Tecan LS Reloaded Open in a separate window Table 2.



Similar Products

96
SCIENION non contact microarray spotter
Overview of the experimental workflow for analyzing retinal antigen profiles captured by serum autoantibodies from patients with different stages of AMD. Antibodies isolated from AMD patient serum were captured on Protein G magnetic beads and incubated overnight with porcine retinal lysates. Unbound retinal antigens were washed away, and antibody-bound antigens were subsequently subjected to MS analysis. Selected differentially expressed retinal antigen targets identified by MS were further evaluated and validated in serum and tear samples from AMD patients using antigen <t>microarray</t> technology. The image was created using BioRender.com ( https://biorender.com/ , accessed on 14. June 2026).
Non Contact Microarray Spotter, supplied by SCIENION, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sciflexarrayer+s3+non-contact+microarray/sciFLEXARRAYER+S3/pmc13388467-92-19-24
Average 96 stars, based on 1 article reviews
non contact microarray spotter - by Bioz Stars, 2026-11
96/100 stars
  Buy from Supplier

96
SCIENION sciflexarrayer s3 non contact microarray
Overview of the experimental workflow for analyzing retinal antigen profiles captured by serum autoantibodies from patients with different stages of AMD. Antibodies isolated from AMD patient serum were captured on Protein G magnetic beads and incubated overnight with porcine retinal lysates. Unbound retinal antigens were washed away, and antibody-bound antigens were subsequently subjected to MS analysis. Selected differentially expressed retinal antigen targets identified by MS were further evaluated and validated in serum and tear samples from AMD patients using antigen <t>microarray</t> technology. The image was created using BioRender.com ( https://biorender.com/ , accessed on 14. June 2026).
Sciflexarrayer S3 Non Contact Microarray, supplied by SCIENION, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sciflexarrayer+s3+non-contact+microarray/sciFLEXARRAYER+S3/pm41674152-206-25-24
Average 96 stars, based on 1 article reviews
sciflexarrayer s3 non contact microarray - by Bioz Stars, 2026-11
96/100 stars
  Buy from Supplier

Image Search Results


Overview of the experimental workflow for analyzing retinal antigen profiles captured by serum autoantibodies from patients with different stages of AMD. Antibodies isolated from AMD patient serum were captured on Protein G magnetic beads and incubated overnight with porcine retinal lysates. Unbound retinal antigens were washed away, and antibody-bound antigens were subsequently subjected to MS analysis. Selected differentially expressed retinal antigen targets identified by MS were further evaluated and validated in serum and tear samples from AMD patients using antigen microarray technology. The image was created using BioRender.com ( https://biorender.com/ , accessed on 14. June 2026).

Journal: Frontiers in Immunology

Article Title: Stage-associated immunoproteomic profiling of serum autoantibody-captured retinal antigens in age-related macular degeneration

doi: 10.3389/fimmu.2026.1824524

Figure Lengend Snippet: Overview of the experimental workflow for analyzing retinal antigen profiles captured by serum autoantibodies from patients with different stages of AMD. Antibodies isolated from AMD patient serum were captured on Protein G magnetic beads and incubated overnight with porcine retinal lysates. Unbound retinal antigens were washed away, and antibody-bound antigens were subsequently subjected to MS analysis. Selected differentially expressed retinal antigen targets identified by MS were further evaluated and validated in serum and tear samples from AMD patients using antigen microarray technology. The image was created using BioRender.com ( https://biorender.com/ , accessed on 14. June 2026).

Article Snippet: Commercially obtained antigens ( ) were spotted in triplicates onto nitrocellulose-coated slides (Oncyte, Grace Bio Labs, USA) using a non-contact microarray spotter (sciFLEXARRAYER S3, Scienion, Germany).

Techniques: Isolation, Magnetic Beads, Incubation, Microarray

Selection and validation of candidate retinal antigens recognized by serum and tear autoantibodies in patients with different stages of AMD. (A) Venn diagram showing shared and stage-specific differentially enriched retinal antigens captured by serum autoantibodies across AMD subgroups. (B) Heatmap showing the relative abundance of selected candidate retinal antigens across AMD stages. Red indicates increased abundance and blue indicates decreased abundance. Candidate antigens selected for further validation are highlighted with red boxes. (C–H) Antigen microarray validation of serum and tear autoantibody reactivity against selected retinal antigens, including PSMD6, HSPA12A, PTGES3, ATP5A1, PFKM, and SDHB, in CTRL and AMD subgroups. Data are presented as mean ± SE. *p < 0.05.

Journal: Frontiers in Immunology

Article Title: Stage-associated immunoproteomic profiling of serum autoantibody-captured retinal antigens in age-related macular degeneration

doi: 10.3389/fimmu.2026.1824524

Figure Lengend Snippet: Selection and validation of candidate retinal antigens recognized by serum and tear autoantibodies in patients with different stages of AMD. (A) Venn diagram showing shared and stage-specific differentially enriched retinal antigens captured by serum autoantibodies across AMD subgroups. (B) Heatmap showing the relative abundance of selected candidate retinal antigens across AMD stages. Red indicates increased abundance and blue indicates decreased abundance. Candidate antigens selected for further validation are highlighted with red boxes. (C–H) Antigen microarray validation of serum and tear autoantibody reactivity against selected retinal antigens, including PSMD6, HSPA12A, PTGES3, ATP5A1, PFKM, and SDHB, in CTRL and AMD subgroups. Data are presented as mean ± SE. *p < 0.05.

Article Snippet: Commercially obtained antigens ( ) were spotted in triplicates onto nitrocellulose-coated slides (Oncyte, Grace Bio Labs, USA) using a non-contact microarray spotter (sciFLEXARRAYER S3, Scienion, Germany).

Techniques: Selection, Biomarker Discovery, Microarray

Validation and overlap analysis of selected candidate retinal antigens recognized by serum and tear autoantibodies in patients with different stages of AMD. (A) Venn diagram showing shared and stage-specific differentially enriched retinal antigens captured by serum autoantibodies across AMD subgroups. (B–G) A subset of candidate retinal antigen targets identified by serum autoantibody-based proteomic profiling, including FTH1, SAG, VIM, PRMT5, SEC22B, and HIST2H2AA3, was selected for further validation by antigen microarray analysis in serum and tear samples from CTRL and AMD subgroups. Data are presented as mean ± SE, with whiskers indicating 1.96 × SE.*p < 0.05. (H) Venn diagram showing the overlap among serum proteomics-positive results, serum autoantibody microarray-positive results, and tear autoantibody microarray-positive results. The antigen microarray analysis was performed as a targeted validation of selected MS-derived retinal antigen targets. Candidate antigens supported by multiple datasets are highlighted in red.

Journal: Frontiers in Immunology

Article Title: Stage-associated immunoproteomic profiling of serum autoantibody-captured retinal antigens in age-related macular degeneration

doi: 10.3389/fimmu.2026.1824524

Figure Lengend Snippet: Validation and overlap analysis of selected candidate retinal antigens recognized by serum and tear autoantibodies in patients with different stages of AMD. (A) Venn diagram showing shared and stage-specific differentially enriched retinal antigens captured by serum autoantibodies across AMD subgroups. (B–G) A subset of candidate retinal antigen targets identified by serum autoantibody-based proteomic profiling, including FTH1, SAG, VIM, PRMT5, SEC22B, and HIST2H2AA3, was selected for further validation by antigen microarray analysis in serum and tear samples from CTRL and AMD subgroups. Data are presented as mean ± SE, with whiskers indicating 1.96 × SE.*p < 0.05. (H) Venn diagram showing the overlap among serum proteomics-positive results, serum autoantibody microarray-positive results, and tear autoantibody microarray-positive results. The antigen microarray analysis was performed as a targeted validation of selected MS-derived retinal antigen targets. Candidate antigens supported by multiple datasets are highlighted in red.

Article Snippet: Commercially obtained antigens ( ) were spotted in triplicates onto nitrocellulose-coated slides (Oncyte, Grace Bio Labs, USA) using a non-contact microarray spotter (sciFLEXARRAYER S3, Scienion, Germany).

Techniques: Biomarker Discovery, Microarray, Derivative Assay