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mouse scf  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec mouse scf

    Mouse Scf, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/scf/Mouse+SCF%2C+research+grade/pmc09256946-63-0-5
    Average 93 stars, based on 18 article reviews
    mouse scf - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "Generation of hematopoietic lineage cell-specific chimeric mice using retrovirus-transduced fetal liver cells"

    Article Title: Generation of hematopoietic lineage cell-specific chimeric mice using retrovirus-transduced fetal liver cells

    Journal: STAR Protocols

    doi: 10.1016/j.xpro.2022.101526


    Figure Legend Snippet:

    Techniques Used: Virus, Recombinant, Marker, Modification, Saline, Transfection, Western Blot, Plasmid Preparation, Purification, Gel Extraction, Retroviral, Sequencing, Software, Flow Cytometry

    Related Articles

    other:

    Article Title: The human and mouse dendritic cell receptor DCIR binds to LRP1 N-glycans containing terminal galactoses, including the α-Gal antigen
    Article Snippet: Blood was collected by cardiac puncture in 1.5 ml tubes containing 40 μL of 0.5 mM EDTA (Euromedex) and cells were isolated after two steps of red blood cells lysis (160 mM NH 4 Cl (Sigma); 10mM KHCO3 (Sigma) and 0.1 mM EDTA).

    Incubation:

    Article Title: Senataxin promotes recombination fidelity during antigen receptor gene diversification.
    Article Snippet: Antigen receptor diversity depends on the assembly of variable (V), diverse (D), and joining (J) exons in genes encoding immunoglobulins (Igs) and T cell receptors (TCRs).. During V(D)J recombination, DNA doublestrand breaks (DSBs) introduced by the RAG1/2 nuclease complex are repaired by the process of nonhomologous endjoining (NHEJ).. We hypothesized that functional redundancies between NHEJ and the chromatin DSB response, which depends on the kinase ATM, potentially masked the activity of additional factors that regulate V(D)J recombination.

    Cell Culture:

    Article Title: Germline variant GFI1-36N affects DNA repair and sensitizes AML cells to DNA damage and repair therapy.
    Article Snippet: Lin- cells were then cultured in 157 IMDM media containing 20% FBS, 1% Pen/Step, 10 ng/ml mIL-3, 10 ng/ml mIL-6 and 20 158 ng/ml mSCF (Miltenyi Biotec, Cat#130-101-741, Cat#130-096-687, Cat#130-094-065) for 159 expansion. .. Lin- cells were then cultured in 157 IMDM media containing 20% FBS, 1% Pen/Step, 10 ng/ml mIL-3, 10 ng/ml mIL-6 and 20 158 ng/ml mSCF (Miltenyi Biotec, Cat#130-101-741, Cat#130-096-687, Cat#130-094-065) for 159 expansion. ..

    Article Title: Germ line variant GFI1-36N affects DNA repair and sensitizes AML cells to DNA damage and repair therapy
    Article Snippet: Lineage-negative (Lin – ) cells were isolated from the total bone marrow (BM) of knockin mice carrying either human GFI1-36N or human GFI1-36S using the Lineage Cell Depletion Kit (Miltenyi Biotec, catalog no. 130-090-858 and catalog no. 130-042-401). .. Lin – cells were then cultured in IMDM media containing 20% fetal bovine serum, 1% penicillin/streptomycyn, 10 ng/ml murine interleukin-3 (mIL-3), 10 ng/mL mIL-6, and 20 ng/ml murine stem cell factor (Miltenyi Biotec, catalog no. 130-101-741, catalog no. 130-096-687, and catalog no. 130-094-065) for expansion. ..

    Modification:

    Article Title: Estradiol Augments Tumor-Induced Neutrophil Production to Promote Tumor Cell Actions in Lymphangioleiomyomatosis Models.
    Article Snippet: Lymphangioleiomyomatosis (LAM) is a rare cystic lung disease caused by smooth muscle cell-like tumors containing tuberous sclerosis (TSC) gene mutations and found almost exclusively in females.. Patient studies suggest LAM progression is estrogen dependent, an observation supported by in vivo mouse models.. However, in vitro data using TSC-null cell lines demonstrate modest estradiol (E2) responses, suggesting E2 effects in vivo may involve pathways independent of direct tumor stimulation.

    Recombinant:

    Article Title: Estradiol Augments Tumor-Induced Neutrophil Production to Promote Tumor Cell Actions in Lymphangioleiomyomatosis Models.
    Article Snippet: Lymphangioleiomyomatosis (LAM) is a rare cystic lung disease caused by smooth muscle cell-like tumors containing tuberous sclerosis (TSC) gene mutations and found almost exclusively in females.. Patient studies suggest LAM progression is estrogen dependent, an observation supported by in vivo mouse models.. However, in vitro data using TSC-null cell lines demonstrate modest estradiol (E2) responses, suggesting E2 effects in vivo may involve pathways independent of direct tumor stimulation.



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