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Amaxa i-scei expression vector (pcbasce
I Scei Expression Vector (Pcbasce, supplied by Amaxa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/scei+expression+vector+pcbasce/s3c+expression+plasmid/pm19680010-49-3-14
Average 90 stars, based on 1 article reviews
i-scei expression vector (pcbasce - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Short-term calorie restriction enhances DNA repair by non-homologous end joining in mice
Article Snippet: One million primary cultured cells were transfected with 5 μg pCMV-I-SceI plasmid to induce DNA DSBs and 0.1 μg pCMV-DsRed plasmid to normalize transfection efficiency, using program T-020 Amaxa Nucleofector II.

Article Title: Chemical Inhibition of RPA by HAMNO Alters Cell Cycle Dynamics by Impeding DNA Replication and G2-to-M Transition but Has Little Effect on the Radiation-Induced DNA Damage Response
Article Snippet: In total, 1 × 10 6 cells were nucleofected with 1 μg I-SceI-expressing plasmid using Amaxa’s Nucleofector (Lonza Bioscience, Basel, Switzerland).

Article Title: Dose-dependent effects of histone methyltransferase NSD2 on site-specific double-strand break repair.
Article Snippet: Briefly, I-SceI expression vectors (50 μg) were electroporated into 5 106 TK6 cells using AmaxaTM nucleofector (Amaxa Biosystems).

Article Title: Modulation of the poly (ADP-ribose) polymerase inhibitor response and DNA recombination in breast cancer cells by drugs affecting endogenous wild-type p53.
Article Snippet: Synthetic lethal interactions between poly (ADP-ribose) polymerase (PARP) and homologous recombination (HR) repair pathways have been exploited for the development of novel monoand combination cancer therapies.. The tumor suppressor p53 was demonstrated to exhibit indirect and direct regulatory activities in DNA repair, particularly in DNA double-strand break (DSB)-induced and replication-associated HR.. In this study, we tested a potential influence of the p53 status on the response to PARP inhibition, which is known to cause replication stress.

Article Title: An approach to estimate radioadaptation from DSB repair efficiency.
Article Snippet: We introduced the I-SceI expression vector (pCBASce) by electroporation methodology using Nucleofector Kit V (amaxa AG, Cologne, Germany) (Fig. 3).36–38) The I-SceI expression vector was introduced into about 65% of the cells at 24 hr after the transfection and the expression last for 3 days incubation.37) The relatively long expression allowed us to succeed in estimating the influence of low-dose, lowdose-rate γ-rays irradiation on DSB repair, especially the effect of post-IR-exposure, as described below.

Expressing:


Article Title: Tumor-derived Mutations in the Gene Associated with Retinoid Interferon-induced Mortality (GRIM-19) Disrupt Its Anti-signal Transducer and Activator of Transcription 3 (STAT3) Activity and Promote Oncogenesis
Article Snippet: .. Cells were electroporated with the S3C expression plasmid using the Nucleofector technology (Amaxa, Inc). ..

Plasmid Preparation:


Article Title: Tumor-derived Mutations in the Gene Associated with Retinoid Interferon-induced Mortality (GRIM-19) Disrupt Its Anti-signal Transducer and Activator of Transcription 3 (STAT3) Activity and Promote Oncogenesis
Article Snippet: .. Cells were electroporated with the S3C expression plasmid using the Nucleofector technology (Amaxa, Inc). ..



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