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ScienCell sc basal medium
Sc Basal Medium, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sc+medium/sc+medium/pm35536601-88-5-7
Average 90 stars, based on 1 article reviews

sc basal medium - by Bioz Stars, 2026-10

90/100 stars

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Isolation:

Article Title: Melanoma-associated repair-like Schwann cells suppress anti-tumor T-cells via 12/15-LOX/COX2-associated eicosanoid production
Article Snippet: .. All studies were conducted in accordance with the NIH guidelines for the Care and Use of Laboratory Animals and approved by the Institutional Animal Care and Use Committee of the University of Pittsburgh (Pittsburgh, PA) Primary cells and cell lines Primary tdTomato+p75NTR+ SCs were isolated by FACS from fresh skin or sciatic nerves according to a previously described protocol and cultured in SC medium (ScienCell) with 1% (v/v) SC growth supplement (ScienCell), 1% (v/v) penicillin/streptomycin solution, and 5% FBS at 37C, 5% CO2.7,26 Briefly, sciatic nerves were dissected under sterile conditions, incubated in SC medium at 37C for 5 days, dissociated using 1 mg/mL NB4G collagenase (Sigma) in 3 mL HBSS at 37C for 45 min, cut into 1-mm segments and kept in NB4G collagenase at 37C for 30 min. After centrifugation (400×g, 5 min), pellets were resuspended in 1 mL SC medium, and the nerves were triturated until they were completely dissociated. .. Cells were placed in SC medium supplemented with 2 μM forskolin (Sigma) and 50 ng/ mL heregulin-β1 (Sigma) and cultured in poly-D-lysine precoated flasks for 4 days followed by additional 7 days in SC medium without forskolin and heregulin.

Article Title: Melanoma-associated repair-like Schwann cells suppress anti-tumor T-cells via 12/15-LOX/COX2-associated eicosanoid production
Article Snippet: .. Primary tdTomato+p75 NTR+ SCs were isolated by FACS from fresh skin or sciatic nerves according to a previously described protocol and cultured in SC medium (ScienCell) with 1% (v/v) SC growth supplement (ScienCell), 1% (v/v) penicillin/streptomycin solution, and 5% FBS at 37C, 5% CO 2 . , Briefly, sciatic nerves were dissected under sterile conditions, incubated in SC medium at 37C for 5 days, dissociated using 1 mg/mL NB4G collagenase (Sigma) in 3 mL HBSS at 37C for 45 min, cut into 1-mm segments and kept in NB4G collagenase at 37C for 30 min. After centrifugation (400× g , 5 min), pellets were resuspended in 1 mL SC medium, and the nerves were triturated until they were completely dissociated. .. Cells were placed in SC medium supplemented with 2 μM forskolin (Sigma) and 50 ng/mL heregulin-β1 (Sigma) and cultured in poly-D-lysine precoated flasks for 4 days followed by additional 7 days in SC medium without forskolin and heregulin.

FACS:

Article Title: Melanoma-associated repair-like Schwann cells suppress anti-tumor T-cells via 12/15-LOX/COX2-associated eicosanoid production
Article Snippet: .. Primary tdTomato+p75 NTR+ SCs were isolated by FACS from fresh skin or sciatic nerves according to a previously described protocol and cultured in SC medium (ScienCell) with 1% (v/v) SC growth supplement (ScienCell), 1% (v/v) penicillin/streptomycin solution, and 5% FBS at 37C, 5% CO 2 . , Briefly, sciatic nerves were dissected under sterile conditions, incubated in SC medium at 37C for 5 days, dissociated using 1 mg/mL NB4G collagenase (Sigma) in 3 mL HBSS at 37C for 45 min, cut into 1-mm segments and kept in NB4G collagenase at 37C for 30 min. After centrifugation (400× g , 5 min), pellets were resuspended in 1 mL SC medium, and the nerves were triturated until they were completely dissociated. .. Cells were placed in SC medium supplemented with 2 μM forskolin (Sigma) and 50 ng/mL heregulin-β1 (Sigma) and cultured in poly-D-lysine precoated flasks for 4 days followed by additional 7 days in SC medium without forskolin and heregulin.

Cell Culture:

Article Title: The circ_0002538/miR-138-5p/plasmolipin axis regulates Schwann cell migration and myelination in diabetic peripheral neuropathy.
Article Snippet: Next, the nerve segments were cut into 2-mm3 pieces and transferred to a mixture containing Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific), 10% fetal calf serum, 0.125% type IV collagenase (Sigma-Aldrich, St. Louis, MO, USA), 1.25 U/mL dispase II (Solaribo, Beijing, China), and 1% penicillinstreptomycin to digest for 18–20 hours. .. The cells were cultured in SC medium (ScienCell, Carlsbad, CA, USA). .. The SCs used in the other experiments were purchased from ScienCell Research Laboratories and cultured in SC medium containing 5% fetal calf serum.

Article Title: The circ_0002538/miR-138-5p/plasmolipin axis regulates Schwann cell migration and myelination in diabetic peripheral neuropathy.
Article Snippet: The cells were cultured in SC medium (ScienCell, Carlsbad, CA, USA). .. The SCs used in the other experiments were purchased from ScienCell Research Laboratories and cultured in SC medium containing 5% fetal calf serum. .. We added oxidized low-density lipoprotein (ox-LDL, BioVision, Exton, PA, USA) to the culture medium to mimic diabetic conditions.

Article Title: Melanoma-associated repair-like Schwann cells suppress anti-tumor T-cells via 12/15-LOX/COX2-associated eicosanoid production
Article Snippet: .. Primary tdTomato+p75 NTR+ SCs were isolated by FACS from fresh skin or sciatic nerves according to a previously described protocol and cultured in SC medium (ScienCell) with 1% (v/v) SC growth supplement (ScienCell), 1% (v/v) penicillin/streptomycin solution, and 5% FBS at 37C, 5% CO 2 . , Briefly, sciatic nerves were dissected under sterile conditions, incubated in SC medium at 37C for 5 days, dissociated using 1 mg/mL NB4G collagenase (Sigma) in 3 mL HBSS at 37C for 45 min, cut into 1-mm segments and kept in NB4G collagenase at 37C for 30 min. After centrifugation (400× g , 5 min), pellets were resuspended in 1 mL SC medium, and the nerves were triturated until they were completely dissociated. .. Cells were placed in SC medium supplemented with 2 μM forskolin (Sigma) and 50 ng/mL heregulin-β1 (Sigma) and cultured in poly-D-lysine precoated flasks for 4 days followed by additional 7 days in SC medium without forskolin and heregulin.

Article Title: Melittin Alleviates Oxidative Stress Injury in Schwann Cells by Targeting Interleukin-1 Receptor Type 1 to Downregulate Nuclear Factor Kappa B-Mediated Inflammatory Response In Vitro
Article Snippet: Commercial rat SCs of the CM-M111 cell line were purchased from Procell Life Science & Technology, China. .. The SCs were cultured in a SC medium (ScienCell, Carlsbad, CA) comprising a base medium of 5% fetal bovine serum, 1% SC growth supplement, 1% penicillin, and 1% streptomycin. ..

Article Title: The circ_0002538/miR-138-5p/plasmolipin axis regulates Schwann cell migration and myelination in diabetic peripheral neuropathy
Article Snippet: Next, the nerve segments were cut into 2-mm 3 pieces and transferred to a mixture containing Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific), 10% fetal calf serum, 0.125% type IV collagenase (Sigma-Aldrich, St. Louis, MO, USA), 1.25 U/mL dispase II (Solaribo, Beijing, China), and 1% penicillin-streptomycin to digest for 18–20 hours. .. The cells were cultured in SC medium (ScienCell, Carlsbad, CA, USA). .. The SCs used in the other experiments were purchased from ScienCell Research Laboratories and cultured in SC medium containing 5% fetal calf serum.

Sterility:

Article Title: Melanoma-associated repair-like Schwann cells suppress anti-tumor T-cells via 12/15-LOX/COX2-associated eicosanoid production
Article Snippet: .. Primary tdTomato+p75 NTR+ SCs were isolated by FACS from fresh skin or sciatic nerves according to a previously described protocol and cultured in SC medium (ScienCell) with 1% (v/v) SC growth supplement (ScienCell), 1% (v/v) penicillin/streptomycin solution, and 5% FBS at 37C, 5% CO 2 . , Briefly, sciatic nerves were dissected under sterile conditions, incubated in SC medium at 37C for 5 days, dissociated using 1 mg/mL NB4G collagenase (Sigma) in 3 mL HBSS at 37C for 45 min, cut into 1-mm segments and kept in NB4G collagenase at 37C for 30 min. After centrifugation (400× g , 5 min), pellets were resuspended in 1 mL SC medium, and the nerves were triturated until they were completely dissociated. .. Cells were placed in SC medium supplemented with 2 μM forskolin (Sigma) and 50 ng/mL heregulin-β1 (Sigma) and cultured in poly-D-lysine precoated flasks for 4 days followed by additional 7 days in SC medium without forskolin and heregulin.

Incubation:

Article Title: Melanoma-associated repair-like Schwann cells suppress anti-tumor T-cells via 12/15-LOX/COX2-associated eicosanoid production
Article Snippet: .. Primary tdTomato+p75 NTR+ SCs were isolated by FACS from fresh skin or sciatic nerves according to a previously described protocol and cultured in SC medium (ScienCell) with 1% (v/v) SC growth supplement (ScienCell), 1% (v/v) penicillin/streptomycin solution, and 5% FBS at 37C, 5% CO 2 . , Briefly, sciatic nerves were dissected under sterile conditions, incubated in SC medium at 37C for 5 days, dissociated using 1 mg/mL NB4G collagenase (Sigma) in 3 mL HBSS at 37C for 45 min, cut into 1-mm segments and kept in NB4G collagenase at 37C for 30 min. After centrifugation (400× g , 5 min), pellets were resuspended in 1 mL SC medium, and the nerves were triturated until they were completely dissociated. .. Cells were placed in SC medium supplemented with 2 μM forskolin (Sigma) and 50 ng/mL heregulin-β1 (Sigma) and cultured in poly-D-lysine precoated flasks for 4 days followed by additional 7 days in SC medium without forskolin and heregulin.

Centrifugation:

Article Title: Melanoma-associated repair-like Schwann cells suppress anti-tumor T-cells via 12/15-LOX/COX2-associated eicosanoid production
Article Snippet: .. Primary tdTomato+p75 NTR+ SCs were isolated by FACS from fresh skin or sciatic nerves according to a previously described protocol and cultured in SC medium (ScienCell) with 1% (v/v) SC growth supplement (ScienCell), 1% (v/v) penicillin/streptomycin solution, and 5% FBS at 37C, 5% CO 2 . , Briefly, sciatic nerves were dissected under sterile conditions, incubated in SC medium at 37C for 5 days, dissociated using 1 mg/mL NB4G collagenase (Sigma) in 3 mL HBSS at 37C for 45 min, cut into 1-mm segments and kept in NB4G collagenase at 37C for 30 min. After centrifugation (400× g , 5 min), pellets were resuspended in 1 mL SC medium, and the nerves were triturated until they were completely dissociated. .. Cells were placed in SC medium supplemented with 2 μM forskolin (Sigma) and 50 ng/mL heregulin-β1 (Sigma) and cultured in poly-D-lysine precoated flasks for 4 days followed by additional 7 days in SC medium without forskolin and heregulin.

other:

Article Title: Schwann cell-derived exosomes ameliorate peripheral neuropathy induced by ablation of dicer in Schwann cells
Article Snippet: SCs at passage 3–5 were cultured in SC medium (1701, ScienCell).

Article Title: PCSK9 deficiency promotes the development of peripheral neuropathy
Article Snippet: SCs were resuspended in primary SC medium (ScienCell, 1701) supplemented with 1 μM insulin, 2 μM forskolin, and 10ng/mL Nrg1β ( ).

Review