crude nuclear extract (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology
crude nuclear extract
Crude Nuclear Extract, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/salt+buffer/ChIP+Lysis+Buffer+High+Salt/ppr0246731-118-18-29
Average 93 stars, based on 47 article reviews
Crude Nuclear Extract, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/salt+buffer/ChIP+Lysis+Buffer+High+Salt/ppr0246731-118-18-29
Average 93 stars, based on 47 article reviews
crude nuclear extract - by Bioz Stars,
2026-10
93/100 stars
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Incubation:Article Title: Restoration of myogenesis in ALS-myocytes through miR-26a-5p-mediated Smad4 inhibition and its impact on motor neuron development. Article Snippet: Briefly, chromatin was precleared by adding 50 μl Protein A/G 779 PLUS-Agarose (Santa Crus, sc-2003) and incubated for 30 minutes at 4° C. After the solution was 780 centrifugated at full speed for 5 minutes at 4° C and the supernatant transferred to a new tube where 781 the primary antibody was added. .. After an overnight incubation at 4° C 50 μl of Protein A/G PLUS-782 Agarose were added and incubated for 2 h at 4° C. Beads were harvested by centrifugations at 12,000 783 rpm for 20 sec. Beads were washed twice with 1 ml of Article Title: Aβ-induced mitochondrial dysfunction in neural progenitors controls KDM5A to influence neuronal differentiation Article Snippet: .. Cell pellets were suspended with Lysis:Article Title: Restoration of myogenesis in ALS-myocytes through miR-26a-5p-mediated Smad4 inhibition and its impact on motor neuron development. Article Snippet: Briefly, chromatin was precleared by adding 50 μl Protein A/G 779 PLUS-Agarose (Santa Crus, sc-2003) and incubated for 30 minutes at 4° C. After the solution was 780 centrifugated at full speed for 5 minutes at 4° C and the supernatant transferred to a new tube where 781 the primary antibody was added. .. After an overnight incubation at 4° C 50 μl of Protein A/G PLUS-782 Agarose were added and incubated for 2 h at 4° C. Beads were harvested by centrifugations at 12,000 783 rpm for 20 sec. Beads were washed twice with 1 ml of Article Title: E protein binding at the Tcra enhancer promotes Tcra repertoire diversity Article Snippet: Cross-linked cells were resuspended at 20 x 10 6 cells/mL in ChIP Lysis Buffer (Santa Cruz Biotechnology, Cat. sc-45000) and incubated 5 min on ice. .. Cross-linked cells were pelleted and resuspended at 10 x 10 6 cells/mL in Article Title: Valemetostat–SAHA-Driven Acetylation of p53 via SET/TAF-Iβ Displacement and p300 Activation Modulates Cell Cycle Regulators in Pancreatic Cancer Cells Article Snippet: .. The nuclei were resuspended in 1.9 mL Article Title: PRMT5 is an actionable therapeutic target in CDK4/6 inhibitor-resistant ER+/RB-deficient breast cancer Article Snippet: .. Cells were lysed with PIPES buffer (5 mM PIPES pH 8.0, 85 mM KCl, 0.5% NP-40; Santa Cruz Biotechnology), and nuclei were isolated by centrifugation and then lysed with Article Title: Restoration of myogenesis in ALS-myocytes through miR-26a-5p-mediated Smad4 inhibition and its impact on motor neuron development Article Snippet: C2C12 myoblasts were transfected with 150 nM of mirVana miRNA mimics or scrambled sequence, purchased from Life Technologies. .. Beads were harvested by centrifugation at 12,000 rpm for 20 s. Beads were washed twice with 1 mL of Article Title: Protein arginine methyltransferase 5 (PRMT5) is an actionable therapeutic target in CDK4/6 inhibitor-resistant ER+/RB-deficient breast cancer Article Snippet: .. Cells were lysed with PIPES buffer (5 mM PIPES pH 8.0, 85 mM KCl, 0.5% NP-40; Santa Cruz Biotechnology), and nuclei were isolated by centrifugation and then lysed with Article Title: Endoplasmic reticulum-mitochondrion disconnection promotes metabolic reprogramming and cystogenesis in polycystic kidney disease Article Snippet: After tissue homogenization by Dounce tissue homogenizer, cells were lysed in lysis buffer (Santa Cruz sc-45000) and nuclei pellets were collected by centrifugation and washed once with ice-cold PBS. .. Pellets were resuspended in high Article Title: Aβ-induced mitochondrial dysfunction in neural progenitors controls KDM5A to influence neuronal differentiation Article Snippet: .. Cell pellets were suspended with Chromatin Immunoprecipitation:Article Title: E protein binding at the Tcra enhancer promotes Tcra repertoire diversity Article Snippet: Cross-linked cells were resuspended at 20 x 10 6 cells/mL in ChIP Lysis Buffer (Santa Cruz Biotechnology, Cat. sc-45000) and incubated 5 min on ice. .. Cross-linked cells were pelleted and resuspended at 10 x 10 6 cells/mL in Article Title: PRMT5 is an actionable therapeutic target in CDK4/6 inhibitor-resistant ER+/RB-deficient breast cancer Article Snippet: .. Cells were lysed with PIPES buffer (5 mM PIPES pH 8.0, 85 mM KCl, 0.5% NP-40; Santa Cruz Biotechnology), and nuclei were isolated by centrifugation and then lysed with Article Title: Protein arginine methyltransferase 5 (PRMT5) is an actionable therapeutic target in CDK4/6 inhibitor-resistant ER+/RB-deficient breast cancer Article Snippet: .. Cells were lysed with PIPES buffer (5 mM PIPES pH 8.0, 85 mM KCl, 0.5% NP-40; Santa Cruz Biotechnology), and nuclei were isolated by centrifugation and then lysed with Sonication:Article Title: Valemetostat–SAHA-Driven Acetylation of p53 via SET/TAF-Iβ Displacement and p300 Activation Modulates Cell Cycle Regulators in Pancreatic Cancer Cells Article Snippet: .. The nuclei were resuspended in 1.9 mL Article Title: Endoplasmic reticulum-mitochondrion disconnection promotes metabolic reprogramming and cystogenesis in polycystic kidney disease Article Snippet: After tissue homogenization by Dounce tissue homogenizer, cells were lysed in lysis buffer (Santa Cruz sc-45000) and nuclei pellets were collected by centrifugation and washed once with ice-cold PBS. .. Pellets were resuspended in high Article Title: Aβ-induced mitochondrial dysfunction in neural progenitors controls KDM5A to influence neuronal differentiation Article Snippet: .. Cell pellets were suspended with Isolation:Article Title: PRMT5 is an actionable therapeutic target in CDK4/6 inhibitor-resistant ER+/RB-deficient breast cancer Article Snippet: .. Cells were lysed with PIPES buffer (5 mM PIPES pH 8.0, 85 mM KCl, 0.5% NP-40; Santa Cruz Biotechnology), and nuclei were isolated by centrifugation and then lysed with Article Title: Protein arginine methyltransferase 5 (PRMT5) is an actionable therapeutic target in CDK4/6 inhibitor-resistant ER+/RB-deficient breast cancer Article Snippet: .. Cells were lysed with PIPES buffer (5 mM PIPES pH 8.0, 85 mM KCl, 0.5% NP-40; Santa Cruz Biotechnology), and nuclei were isolated by centrifugation and then lysed with Centrifugation:Article Title: PRMT5 is an actionable therapeutic target in CDK4/6 inhibitor-resistant ER+/RB-deficient breast cancer Article Snippet: .. Cells were lysed with PIPES buffer (5 mM PIPES pH 8.0, 85 mM KCl, 0.5% NP-40; Santa Cruz Biotechnology), and nuclei were isolated by centrifugation and then lysed with Article Title: Restoration of myogenesis in ALS-myocytes through miR-26a-5p-mediated Smad4 inhibition and its impact on motor neuron development Article Snippet: C2C12 myoblasts were transfected with 150 nM of mirVana miRNA mimics or scrambled sequence, purchased from Life Technologies. .. Beads were harvested by centrifugation at 12,000 rpm for 20 s. Beads were washed twice with 1 mL of Article Title: Protein arginine methyltransferase 5 (PRMT5) is an actionable therapeutic target in CDK4/6 inhibitor-resistant ER+/RB-deficient breast cancer Article Snippet: .. Cells were lysed with PIPES buffer (5 mM PIPES pH 8.0, 85 mM KCl, 0.5% NP-40; Santa Cruz Biotechnology), and nuclei were isolated by centrifugation and then lysed with |