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ice cold incubation buffer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ice cold incubation buffer
    Ice Cold Incubation Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/salt+buffer/Hanks'+Balanced+Salt+Solution/pmc13098237-323-12-20
    Average 99 stars, based on 1 article reviews
    ice cold incubation buffer - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Lysis:

    Article Title: Loss of catalytic activity and impaired proteostasis in guanosine nucleotide-depleted LRRK2
    Article Snippet: .. At 48-72 h post-transfection, cells were subjected to treatment with vehicle, CQ (as previously described) or starvation (16 h serum deprivation followed by 2 h in Hanks’ Balanced Salt Solution, HBSS, Life Technologies) prior to lysis and anti-Flag affinity purification for MS or immunoblot validation. .. For autophagic flux measurements, RAW 264.7 WT, Lrrk2 KO and Lrrk2 T1348N cells were cultured onto 12mm glass coverslips in 24-well plates that had previously been coated with poly-L-lysine (Sigma-Aldrich), and transiently transfected with 1 μg mCherry-GFP-LC3 plasmid DNA per well using Lipofectamine 2000 (Invitrogen) at a DNA:Lipofectamine ratio of 1:2.

    Affinity Purification:

    Article Title: Loss of catalytic activity and impaired proteostasis in guanosine nucleotide-depleted LRRK2
    Article Snippet: .. At 48-72 h post-transfection, cells were subjected to treatment with vehicle, CQ (as previously described) or starvation (16 h serum deprivation followed by 2 h in Hanks’ Balanced Salt Solution, HBSS, Life Technologies) prior to lysis and anti-Flag affinity purification for MS or immunoblot validation. .. For autophagic flux measurements, RAW 264.7 WT, Lrrk2 KO and Lrrk2 T1348N cells were cultured onto 12mm glass coverslips in 24-well plates that had previously been coated with poly-L-lysine (Sigma-Aldrich), and transiently transfected with 1 μg mCherry-GFP-LC3 plasmid DNA per well using Lipofectamine 2000 (Invitrogen) at a DNA:Lipofectamine ratio of 1:2.

    Western Blot:

    Article Title: Loss of catalytic activity and impaired proteostasis in guanosine nucleotide-depleted LRRK2
    Article Snippet: .. At 48-72 h post-transfection, cells were subjected to treatment with vehicle, CQ (as previously described) or starvation (16 h serum deprivation followed by 2 h in Hanks’ Balanced Salt Solution, HBSS, Life Technologies) prior to lysis and anti-Flag affinity purification for MS or immunoblot validation. .. For autophagic flux measurements, RAW 264.7 WT, Lrrk2 KO and Lrrk2 T1348N cells were cultured onto 12mm glass coverslips in 24-well plates that had previously been coated with poly-L-lysine (Sigma-Aldrich), and transiently transfected with 1 μg mCherry-GFP-LC3 plasmid DNA per well using Lipofectamine 2000 (Invitrogen) at a DNA:Lipofectamine ratio of 1:2.

    Biomarker Discovery:

    Article Title: Loss of catalytic activity and impaired proteostasis in guanosine nucleotide-depleted LRRK2
    Article Snippet: .. At 48-72 h post-transfection, cells were subjected to treatment with vehicle, CQ (as previously described) or starvation (16 h serum deprivation followed by 2 h in Hanks’ Balanced Salt Solution, HBSS, Life Technologies) prior to lysis and anti-Flag affinity purification for MS or immunoblot validation. .. For autophagic flux measurements, RAW 264.7 WT, Lrrk2 KO and Lrrk2 T1348N cells were cultured onto 12mm glass coverslips in 24-well plates that had previously been coated with poly-L-lysine (Sigma-Aldrich), and transiently transfected with 1 μg mCherry-GFP-LC3 plasmid DNA per well using Lipofectamine 2000 (Invitrogen) at a DNA:Lipofectamine ratio of 1:2.

    Transferring:

    Article Title: Coupling DNA intercalation with redox catalysis: selective killing mechanism of glioblastoma by daidzin
    Article Snippet: .. The tissues were then transferred to cold Hank’s Balanced Salt Solution (HBSS, Gibco) and mechanically minced using a 5 ml serological pipette. .. Tissue dissociation was enzymatically performed in HBSS containing trypsin (Sigma–Aldrich) (10 mg/ml stock; 0.25 ml per brain) and DNase I (Sigma–Aldrich) (0.1 mg/ml stock, 0.25 ml per brain) to minimize cell clumping caused by released DNA.

    Isolation:

    Article Title: Aryl hydrocarbon receptor in club cells drives Th17-mediated lung injury following inhalation exposure to environmentally persistent free radicals
    Article Snippet: .. The isolated lungs were placed in Hank's Balanced Salt solution (HBSS) containing collagenase I (Invitrogen) and DNase I (Sigma Aldrich, St. Louis, MO), and coarsely pulverized using an octodissociator (Miltenyi, Auburn, CA) and incubated for 20 min in a heater/shaker set at 37 °C and 250 RPM. ..

    Article Title: Longitudinal prediction of drug response in high-grade serous ovarian cancer organoid cultures aligning with clinical responses
    Article Snippet: .. For ascitic fluids, cells in the pellets were isolated by centrifugation at 1000 rpm for 10 min and subsequently washed twice with Hank's Balanced Salt Solution (HBSS, Gibco, Massachusetts, United States). .. To remove erythrocytes from the fluid, cold red blood cell lysis buffer (Roche Diagnostics, Basel, Switzerland) was added and incubated on ice with continuous stirring for 10 min.

    Incubation:

    Article Title: Aryl hydrocarbon receptor in club cells drives Th17-mediated lung injury following inhalation exposure to environmentally persistent free radicals
    Article Snippet: .. The isolated lungs were placed in Hank's Balanced Salt solution (HBSS) containing collagenase I (Invitrogen) and DNase I (Sigma Aldrich, St. Louis, MO), and coarsely pulverized using an octodissociator (Miltenyi, Auburn, CA) and incubated for 20 min in a heater/shaker set at 37 °C and 250 RPM. ..

    Centrifugation:

    Article Title: Longitudinal prediction of drug response in high-grade serous ovarian cancer organoid cultures aligning with clinical responses
    Article Snippet: .. For ascitic fluids, cells in the pellets were isolated by centrifugation at 1000 rpm for 10 min and subsequently washed twice with Hank's Balanced Salt Solution (HBSS, Gibco, Massachusetts, United States). .. To remove erythrocytes from the fluid, cold red blood cell lysis buffer (Roche Diagnostics, Basel, Switzerland) was added and incubated on ice with continuous stirring for 10 min.



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