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alkaline phosphatase substrate buffer containing nitro-blue tetrazolium chloride/5-bromo-4-chloro-3’-indolyphosphate p-toluidine salt  (Promega)

 
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  • 90

    Structured Review

    Promega alkaline phosphatase substrate buffer containing nitro-blue tetrazolium chloride/5-bromo-4-chloro-3’-indolyphosphate p-toluidine salt
    Alkaline Phosphatase Substrate Buffer Containing Nitro Blue Tetrazolium Chloride/5 Bromo 4 Chloro 3’ Indolyphosphate P Toluidine Salt, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/salt+buffer/alkaline+phosphatase+substrate+buffer+containing+nitro+blue+tetrazolium+chloride+5+bromo+4+chloro+3%E2%80%99+indolyphosphate+p+toluidine+salt/pmc03709059-62-81-88
    Average 90 stars, based on 1 article reviews
    alkaline phosphatase substrate buffer containing nitro-blue tetrazolium chloride/5-bromo-4-chloro-3’-indolyphosphate p-toluidine salt - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Membrane:

    Article Title: Monoclonal antibody-based serological methods for maize chlorotic mottle virus detection in China
    Article Snippet: .. The dried membrane was blocked with 5% skimmed milk (0.05 g/ml) for 30–60 min, and then incubated in suitably diluted MAb for 1 h. After washing four times with 0.01 mol/L PBS containing 0.05% Tween-20 (PBST), the membrane was incubated in a suitable dilution of goat anti-mouse IgG conjugated with alkaline phosphatase (Sigma-Aldrich, St. Louis, MO, USA) for 1 h. Finally, the membrane was washed five times with PBST, and then incubated to be color-developed in alkaline phosphatase substrate buffer containing nitro-blue tetrazolium chloride/5-bromo-4-chloro-3′-indolyphosphate p -toluidine salt (NBT/BCIP; Promega, Madison, WI, USA). ..

    Incubation:

    Article Title: Monoclonal antibody-based serological methods for maize chlorotic mottle virus detection in China
    Article Snippet: .. The dried membrane was blocked with 5% skimmed milk (0.05 g/ml) for 30–60 min, and then incubated in suitably diluted MAb for 1 h. After washing four times with 0.01 mol/L PBS containing 0.05% Tween-20 (PBST), the membrane was incubated in a suitable dilution of goat anti-mouse IgG conjugated with alkaline phosphatase (Sigma-Aldrich, St. Louis, MO, USA) for 1 h. Finally, the membrane was washed five times with PBST, and then incubated to be color-developed in alkaline phosphatase substrate buffer containing nitro-blue tetrazolium chloride/5-bromo-4-chloro-3′-indolyphosphate p -toluidine salt (NBT/BCIP; Promega, Madison, WI, USA). ..



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