automacs running buffer (Miltenyi Biotec)

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Miltenyi Biotec automacs running buffer
Automacs Running Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 366 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/running+buffer/autoMACS+Running+Buffer+-+MACS+Separation+Buffer/us12692546-503-17-20
Average 97 stars, based on 366 article reviews

automacs running buffer - by Bioz Stars, 2026-09

97/100 stars

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Centrifugation:

Article Title: A Conserved Chromatin-Driven Checkpoint Defines Late Macrophage Maturation Independent of Tissue Specialization
Article Snippet: .. Cells were harvested, washed with PBS, pelleted by centrifugation (2,200 rpm, 5 min, 4 °C), and resuspended in autoMACS Running Buffer (Miltenyi Biotec, cat. no. 130-091-221) containing Fc receptor blocking antibody (anti-CD16/CD32; BD Pharmingen, clone 2.4G2, cat. no. 553141). ..

Blocking Assay:

other:

Article Title: Protocol for isolation of primary hepatic lymphatic endothelial cells from rat liver.
Article Snippet: Antibodies BD OptiBuildTM BV421 Mouse Anti-Rat CD45R (dilution 0.7μl/100μl) BD Biosciences Clone: HIS24; Cat# 743590; RRID: AB_2741603 PE Mouse Anti-Rat CD31 (dilution 1μl/100μl) BD Pharmingen Clone: TLD-3A12; Cat# 555027; RRID: AB_395657 LYVE-1 (E3L3V) Rabbit Monoclonal Antibody (dilution 0.625μl/100μl) Cell Signaling Technology Clone: E3L3V; Cat# 67538S Alexa Fluor® 647 anti-rat Podoplanin (PDPN) Antibody (dilution 0.6μl/100μl) BioLegend Clone: 8.1.1; Cat# 250906; RRID: AB_2814106 Purified anti-Prox1 Antibody (dilution 0.625μl/100μl) BioLegend Clone: Poly19051; Cat# 696902; RRID: AB_2687107 Purified anti-mouse VEGFR-3 (FLT-4) Antibody (dilution 1μl/100μl) BioLegend Clone: AFL4; Cat# 140901; RRID: AB_10683463 Anti-rat CD146 (MCAM)-PE Antibody (dilution 0.6μL/100 μL) BD PharmingenTM Clone: ME-9F1; Cat# 562231; RRID: AB_11154231 CD45 MicroBeads, rat (dilution 20μl/107cells) Miltenyi Biotec Cat# 130-109-682 Chemicals, peptides, and recombinant proteins Collagenase Type I Gibco Cat#17100017 DNase I Invitrogen (Thermo Fisher Scientific) Cat# 18947019 Pronase Roche Cat# 101659221001 DMEM F12 Gibco Cat# 11320033 RPMI Media Gibco Cat# 11875093 7-AAD Invitrogen Cat# A1310 Fetal Bovine Serum (FBS) Gibco Cat# 10270106 Fibronectin Sigma-Aldrich Cat# F0895 Endothelial Cell Media PromoCell Cat# C-39211 VEGFC Growth factors MedChemExpress Cat# HY-P7313 Penicillin-Streptomycin solution (10,000 U/mL) Gibco Cat# 15140122 Dulbecco’s Phosphate Buffered Saline HiMedia Cat# TS1006-10X1L Trypan Blue Solution Sigma-Aldrich Cat# T6146-25G Percoll Cytiva Cat# 17089101 RBC Lysis Buffer eBioscience Cat# 00-4333-57 Ketamine BNM Organics N/A Xylazine BNM Organics N/A Sheath fluid BD FACS Flow Cat# 342003 FACS Buffer BD Pharmingen Cat# 554656 MACS Buffer Miltenyi Biotec Cat# 130-091-221 Experimental models: Organisms/strains Sprague-Dawley (SD) Rats, Male, 5-6 weeks old, (200-250g) Centre for Comparative Medicine (CCM) Institutional animal facility (ILBS, New Delhi, India) N/A Software and algorithms Fiji (ImageJ), version 2.14.0. https://doi.org/10.1038/ nmeth.2019 https://fiji.sc Flow jo v11.1 FlowJo LLC https://www.flowjo.com/ Other FACS Tubes ARCK Technologies Cat# 5TAT090A MACS Column LS Miltenyi Biotec Cat# 130-122-729 Serological pipettes 25 mL Thermo Fisher Cat# 170357N 50 mL sterile falcons Corning Cat# 352070 (Continued on next page) 4 STAR Protocols 7, 104728, September 18, 2026

Article Title: Interferon-λ drives renal fibrosis by coordinating epithelial-fibroblast crosstalk.
Article Snippet: Pellets were resuspended in MACS running buffer (130-091-221; Miltenyi Biotec) and incubated for 30 min with biotinylated primary anti-vimentin antibody (Biotin-60330; Proteintech).

Saline:

Article Title: Standardised dual-flow cytometry workflows for quantitative assessment of mitochondrial superoxide and mitochondrial mass in live cancer cells and primary multiple myeloma cells
Article Snippet: MM1S (#CRL-2974, ATCC, Manassas, VA, United States) RPMI-8226 (#CCL-155, ATCC, Manassas, VA, United States) HG3 (#ACC765, DSMZ, Braunschweig, Germany) Patient-derived CD138 + multiple myeloma cells .. RPMI-1640 Medium (#11–875–093, Life Technologies, United States) L–Glutamine (#25030081, Life Technologies, United States) Penicillin-Streptomycin Solution (#15140130, Life Technologies, United States) Fetal Bovine Serum (#A3160502, Life Technologies, United States) Dulbecco’s Phosphate Buffered Saline (#14-190-144, Thermo Fisher Scientific, MA, United States) HEPES (#15630080, Thermo Fisher Scientific, MA, United States) StraightFrom Whole Blood CD138 MicroBeads (#130-093-062, MiltenyiBiotec, CO, Germany) CD138-PE Antibody (#130-127-978, MiltenyiBiotec, CO, Germany) CD19-APC Antibody (#130-113-642, MiltenyiBiotec, CO, Germany) CD45-FITC Antibody (#130-110-631, MiltenyiBiotec, CO, Germany) MACS® Separation Buffer (#130-091-221, MiltenyiBiotec, CO, Germany) Trypsin-EDTA (0.25%) (#25200056, Thermo Fisher Scientific, MA, United States) Trypan Blue Stain (0.4%) (#15250–061, Life Technologies, United States) Dimethyl sulfoxide (DMSO) (#67–68–5, Thermo Fisher Scientific, MA, United States) Hanks’ Balanced Salt Solution (HBSS, with Ca 2+ and Mg 2+ ) (#14025–092, Thermo Fisher Scientific, MA, United States) Invitrogen MitoSOX Green Mitochondrial Superoxide Indicators (# M36005 , Thermo Fisher Scientific, MA, United States) Rotenone (#R8875, Sigma Aldrich-Merck, Germany) Invitrogen MitoTracker Red CMXRos (#M7512, Thermo Fisher Scientific, MA, United States) Propidium Iodide (#P1304MP, Thermo Fisher Scientific, MA, United States) .. Cell culture equipment 4 and −20 °C refrigerators 5 mL Round Bottom Test Tubes (#CLS352052, Sigma Aldrich-Merck, Germany) MACS® SmartStrainers (100 μm) (#130-098-463, MiltenyiBiotec, CO, Germany) Chill 5 Rack (#130-092-951, MiltenyiBiotec, CO, Germany) AutoMACS ProSeparator (#130-092-545, MiltenyiBiotec, CO, Germany) Centrifuge (5810R, Eppendorf, Germany) Vortex Mixer (F202A0175, VELP Scientifica, Italy) Water bath (B3A00035-BTU3, ISCO, Italy) Incubator set at 37 °C with 5% CO 2 (Forma Steri-Cycle, Thermo Fisher Scientific, MA, United States) Microscope (Axio Vert A1, Zeiss, Jena, Germany) DxFLEX flow cytometer (488 nm blue laser and 638–688 nm red laser, for excitation) (Beckman Coulter, IN, United States) Kaluza 2.1 analysis software (Beckman Coulter, IN, United States) GraphPad Prism 10 software (San Diego, CA, United States) Additional reagents and equipment for counting cells.

Magnetic Cell Separation:

Article Title: Clinical MAPPs: a personalized healthcare-driven assay for the direct identification of potential T cell epitopes in patients
Article Snippet: .. Cells were centrifuged twice (300 x g for 5 min at RT) and were re-suspended in 10–50 mL MACS buffer (Miltenyi; Cat: #130-091-221) final volume. .. Monocytes were isolated from PBMCs by positive immune-selection using anti-CD14 microbeads (Miltenyi; Cat: #130-050-201) on LS columns (Miltenyi; Cat: ## 130-042-401) and a QuadroMACS magnetic separator (Miltenyi; Cat: #130-090-976) following the manufacturer’s procedures under sterile conditions.

Staining:

Labeling:

Article Title: Synergistic nanoparticle dyad improving tumor immunogenicity and reshaping tolerogenic dendritic cells in hepatocellular carcinoma
Article Snippet: Spleens were gently dissociated in PBS using a syringe plunger, passed through 70 μm cell strainers, and treated with red blood cell lysis buffer (TIANGEN, RT122). .. Splenocytes were resuspended in autoMACS Running Buffer (Miltenyi, 130-091-221) and labeled with CD8a Microbeads (Miltenyi, 130-117-044). .. CD8+ T cells were isolated using an autoMACS® NEL Separator (Miltenyi) and then activated for 48 h in RPMI-1640 supplemented with anti-mouse CD3ε (5 μg/mL, BioLegend, 100302), anti-mouse CD28 (5 μg/mL, BioLegend, 102102), and IL-2 (100 U/mL, TargetMol, TMPY-02788).

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