sorting buffer (Miltenyi Biotec)

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Miltenyi Biotec sorting buffer
Sorting Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/running+buffer/MACSQuant+Tyto+Running+Buffer/pmc12521654-416-4-6
Average 94 stars, based on 28 article reviews

sorting buffer - by Bioz Stars, 2026-10

94/100 stars

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Incubation:

Article Title: CD133/CD49a discriminate between human pluripotent stem cell-derived pancreatic beta and alpha cells
Article Snippet: .. The cells were then counted, centrifuged, and resuspended in MACSQuant Tyto Running Buffer (Miltenyi Biotec, #130-107-207) supplemented with CD133 antibody (1:10) and CD49a antibody (1:50) (100 μL buffer per 1 × 10 6 cells), and incubated for 15 min at room temperature. .. Subsequently, the cells were washed once with the Running Buffer and sorted on a Miltenyi MACSQuant Tyto sorter using gates to isolate single cells based on scatter characteristics.

Article Title: CD133/CD49a discriminate between human pluripotent stem cell-derived pancreatic beta and alpha cells.
Article Snippet: .. The cells were then counted, centrifuged, and resuspended in MACSQuant Tyto Running Buffer (Miltenyi Biotec, #130-107-207) supplemented with CD133 antibody (1:10) and CD49a antibody (1:50) (100 μL buffer per 1 × 10 6 cells), and incubated for 15 min at room temperature. .. Subsequently, the cells were washed once with the Running Buffer and sorted on a Miltenyi MACSQuant Tyto sorter using gates to isolate single cells based on scatter characteristics.

Article Title: CD31 + Cell Enrichment Enhances Therapeutic Effects of Stromal Vascular Fraction in Experimental Primary Osteoarthritis: A Preclinical Study in the Dunkin Hartley Guinea Pig Model.
Article Snippet: Adipose tissue was collected during general anesthesia via waterjet-assisted liposuction and subsequently processed using the automated Celution 800/CRS system (Cytori Therapeutics, San Diego, CA, US), which employs enzymatic digestion to isolate SVF [ 74 ]. .. To isolate CD31+ cells from SVF, SVF with a concentration of 1 × 108 cells/mL was incubated with a conjugated CD31/APC antibody (Blue Cell Therapeutics Ltd., Denmark) for 10 min. A PBS-based MACSQuant Tyto running buffer (130-107- 206, Miltenyi Biotec, Germany) was used for all washing steps and antibody incubations. .. The labeled SVF was then placed into a MACSQuant Tyto cartridge (130-104-791, Miltenyi Biotec, Germany), and CD31+ cells were sorted using a MACSQuant Tyto cell sorter (Miltenyi Biotec, Germany).

Article Title: A molecular circuit regulates fate plasticity in emerging and adult AT2 cells
Article Snippet: .. Cells are washed with sorting buffer (Miltenyi, 130-107-207) and subsequently incubated with 20 μL of each of the following secondary antibodies: (Anti-rabbit Alexa Fluor 647, anti-rat Alexa Fluor 405) for 30 min at 4 °C. .. Subsequently, the samples are washed, filtered, and resuspended in 1 mL sorting buffer and transferred to the sorting compartment of the cartridge (Miltenyi, 130-104-791).

Concentration Assay:

Article Title: IgG4 + plasma cell enrichment and λ-chain-biased BCR remodeling drive low-grade autoimmunity in chronic obstructive pulmonary disease
Article Snippet: For live B-cell sorting, PBMCs were stained with anti-CD45-FITC (BioLegend, clone HI30), anti-CD19-PerCP-Cy5.5 (BioLegend, clone HIB19) and fixable viability stain 506 (FVS506; BD Horizon) for 30 min on ice in the dark. .. Following staining, cells were washed twice and resuspended in MACSQuant Tyto running buffer (Miltenyi Biotec) at a concentration of 2–4 × 106 cells/mL. .. Cell sorting was performed using a MACSQuant Tyto cell sorter (Miltenyi Biotec) equipped with 405 nm, 488 nm, and 561 nm lasers and a closed cartridge-based system.

Article Title: Automated GMP-compatible production of universal CAR Tregs for organ-targeted tolerance induction.
Article Snippet: The enriched cells were further labeled using MACS GMP clinical-grade antibodies: CD4-Vioblue (Clone M-T466, Miltenyi Biotec) and CD127-FITC (Clone MB15-18C9, Miltenyi Biotec) at 1:31 dilution and CD8APC (Clone BW135/80, Miltenyi Biotec) at either 1:31 or 1:21 dilution for 30 minutes at 4 °C, followed by a single wash with working buffer (300xg, 15 minutes). .. For sorting, the cells were resuspended in 10 mL of MACSQuant Tyto Running Buffer (Miltenyi Biotec) achieving an average concentration of 7.4 × 106 cells/mL (range: 6.9 × 106 to 7.8 × 106). .. The cells were filtered through a 20 μm cellstrainer and loaded into the MACSQuant Tyto high speed (HS) cartridges (Miltenyi Biotec) and sorted on the MACSQuant Tyto Cell Sorter (Miltenyi Biotec).

Staining:

other:

Article Title: A molecular circuit regulates fate plasticity in emerging and adult AT2 cells.
Article Snippet: Single-cell RNA sequencing was performed using the Chromium Single Cell 3’ system (10x Genomics) at the single-cell sequencing core at Mayo Clinic according to manufacturer’s instruction (10x Genomics).

Article Title: Automated GMP-compatible production of universal CAR Tregs for organ-targeted tolerance induction
Article Snippet: The enriched cells were further labeled using MACS GMP clinical-grade antibodies: CD4-Vioblue (Clone M-T466, Miltenyi Biotec) and CD127-FITC (Clone MB15-18C9, Miltenyi Biotec) at 1:31 dilution and CD8-APC (Clone BW135/80, Miltenyi Biotec) at either 1:31 or 1:21 dilution for 30 minutes at 4 °C, followed by a single wash with working buffer (300xg, 15 minutes).

Review