Membrane:Article Title: Laudanosine restricts Ebola virus entry by targeting TPC2-dependent endolysosomal trafficking
Article Snippet: .. The gels were running in 1x MES-SDS running buffer (20x MES-SDS running buffer diluted in deionized water) 30 min at 30 V and subsequently for 80 min at 90 V. Next, the separated proteins were wet blotted onto an Immun-Blot PVDF Membrane using the Thermo Fisher Mini Blot Module system (Cat# B1000) according to the manufacturers instruction and using the transfer buffer (25 mM Tris and 192 mM glycine at a pH of 8.3 with 20% (v/v) methanol) at a constant voltage of 20 V for 1 h. After blocking in Blocker Casein in PBS for 1 h, the proteins on the membrane were stained with primary antibodies diluted in TBS-T (1x TBS with 0.05% (v/v) Tween 20 and 0.1% (v/v) Blocker Casein in PBS) for 2 h at RT or overnight at 4 °C. ..
Article Title: Methods of delivering anionic agents in vivo using non-viral nanoparticle-based delivery systems
Article Snippet: MTS cytotoxicity assay kit was obtained from Promega (Madison, WI, USA). .. Subcloning DH5-alpha competent cells and Lipofectamine 2000, Tracklt 10-bp DNA ladder (0.5 μg/μl), Pre-cast Nupage 4-12% Bis-Tris gels, Nupage MES running buffer, Nupage transfer buffer, Nupage LDS sample buffer (4×), nitrocellulose membrane 0.45 μm, blotting filter paper and magic mark (1 Kb) protein ladder were obtained from Invitrogen (Burlington, ON, Canada). .. Orange dye solution (6×) was obtained from Fermentas (Burlington, ON, Canada). pEGFPataxin1 (E3-82Q) plasmid was obtained from Dr. Zoghbi Huda from Baylor College of Medicine, Houston, USA.
Blocking Assay:Article Title: Laudanosine restricts Ebola virus entry by targeting TPC2-dependent endolysosomal trafficking
Article Snippet: .. The gels were running in 1x MES-SDS running buffer (20x MES-SDS running buffer diluted in deionized water) 30 min at 30 V and subsequently for 80 min at 90 V. Next, the separated proteins were wet blotted onto an Immun-Blot PVDF Membrane using the Thermo Fisher Mini Blot Module system (Cat# B1000) according to the manufacturers instruction and using the transfer buffer (25 mM Tris and 192 mM glycine at a pH of 8.3 with 20% (v/v) methanol) at a constant voltage of 20 V for 1 h. After blocking in Blocker Casein in PBS for 1 h, the proteins on the membrane were stained with primary antibodies diluted in TBS-T (1x TBS with 0.05% (v/v) Tween 20 and 0.1% (v/v) Blocker Casein in PBS) for 2 h at RT or overnight at 4 °C. ..
Article Title: mRNA 3' UTRs chaperone intrinsically disordered regions to control protein activity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Novex Tris-Glycine Mini Protein Gels, 6%, 1.0 mm, 12 well Invitrogen Cat# XP00062 NuPAGE MES SDS running buffer 20x Invitrogen Cat# NP0002 NuPAGE Tris-Acetate SDS running buffer 20x Invitrogen Cat# LA0041 NuPAGE Transfer Buffer (20X) Invitrogen Cat# NP00061 Novex Tris-Glycine SDS running buffer 10x Invitrogen Cat# LC2675 Odyssey blocking buffer (PBS) LI-COR Cat# 927-40000 Polybrene (hexadimethrine bromide) Sigma-Aldrich Cat# H9268 16% Paraformaldehde Aqueous Solution Fisher Scientific Cat# 50-980-487 Digitonin Sigma-Aldrich Cat# D141 SUPERase⋅InTM RNase Inhibitor Invitrogen Cat# AM2694 cOmplete EDTA-free Protease Inhibitor Cocktail Roche Cat# 04693159001 or Cat# 11836170001 PierceTM Anti-HA Magnetic Beads Thermo Fisher Cat# 88836 LIVE/DEADTM Fixable Near-IR Dead Cell Stain Kit Thermo Fisher Cat# L10119 LIVE/DEADTM Fixable Near IR (780) Viability Kit Thermo Fisher Cat# L34994 Puromycin Millipore Sigma Cat# 54-041-1 Cycloheximide Sigma-Aldrich Cat# C7698 Glycine Fisher Scientific Cat# BP381-1 D-Sucrose Fisher Scientific Cat# BP220-1 Ampicillin Sodium Salt Fisher Scientific Cat# BP176025 Kanamycin disulfate salt Sigma-Aldrich Cat# K1876 Sodium Chloride Fisher Scientific Cat# S271-3 Tris base Fisher Scientific Cat# BP152-1 Protein G Dynabeads Thermo Fisher Cat# 10004D KMet SNAP-ChIP Panel Epicypher Cat# 191100 Triton X-100 Fisher Scientific Cat# BP151-100 Tween-20 Fisher Scientific Cat# BP337-500 Na-deoxycholate Fisher Scientific Cat# BP349-100 SPRIselect magnetic beads Beckman Coulter Cat# B23318 Bovine Serum Albumin (BSA) Fisher Scientific Cat# BP1605100 Dextran Sulfate Sodium Salt Spectrum Chemical Cat# DE131 Ribonucleoside Vanadyl Complex NEB Cat# S1402 Salmon testes single stranded DNA Sigma-Aldrich Cat# D9156 Yeast tRNA Life Technologies Cat# 15401029 Formamide Millipore Sigma Cat# S4117 TURBO DNase Life Technologies Cat# AM2238 RNase I Life Technologies Cat# AM2295 Trichloroacetic Acid (TCA) TCI America Cat# T0369500G TALON Metal Affinity Resin Takara Cat# 635503 LB media Fisher Scientific Cat# BP1426-2 IPTG (Isopropyl-β-Dthiogalactopyranoside, dioxane free) RPI research Cat# I56000-25.0 PMSF (Benzylsulfonyl Fluoride) Sigma Aldrich Cat# 78830 Lysozyme Thermo Fisher Cat# 89833 DNase I Sigma-Aldrich Cat# 10104159001 Halt protease inhibitor cocktail Thermo Fisher Cat# 78439 (Continued on next page) ll e2 Cell 189, 1–18.e1–e16, July 23, 2026 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Imidazole Fisher Scientific Cat# O3196-500 Tris(2-carboxyethyl)phosphine hydrochloride (TCEP) Sigma-Aldrich Cat# C4706 Strep-Tactin® 4Flow® high-capacity resin Iba life sciences Cat# 2-1250-010 d-Desthiobiotin Sigma-Aldrich Cat# D1411 Cy5-UTP Enzo life sciences Cat# ENZ-42506 Streptavidin 680 LI-COR Cat# 926-68079 DSG Thermo Fisher Cat# 20593 BS3 (bis(sulfosuccinimidyl)suberate) Thermo Fisher Cat# 21580 ProLong Gold Antifade Mountant with DAPI DNA stain Thermo Fisher Cat# P36935 BMOE (bismaleimidoethane) Thermo Fisher Cat# 22323 Ethanol Fisher Scientific Cat# BP28184 DTT (dithiothreitol) Life Technologies Cat# R0862 Nonidet P-40 Sigma-Aldrich Cat# 74385 HEPES Sigma-Aldrich Cat# H4034 Magnesium Chloride Hexahydrate Fisher Scientific Cat# M33-500 Potassium Chloride Sigma-Aldrich Cat# P9541 Glycerol Sigma-Aldrich Cat# G5516 DMEM, free of phenol red and FBS Thermo Fisher Cat# 21063029 qScript cDNA SuperMix Quantabio Cat# 95048 PowerUp SYBRTM Green Master Mix Thermo Fisher Cat# A25742 4x LDS Sample buffer Thermo Fisher Cat# J61942.AD IRDye® 800CW DBCO Infrared Dye LI-COR Cat# 929-50000 5′ DNA Adenylation Kit NEB Cat# 2610 T4 RNA Ligase 1 (ssRNA Ligase) NEB Cat# M0204 T4 PNK NEB Cat# M0201 RNase A, DNase and protease-free (10 mg/ mL) Thermo Fisher Cat# EN0531 SureCastTM Acrylamide Solution (40%) Thermo Fisher Cat# HC2040 Phos-tag (TM) Acrylamide AAL-107 FUJIFILM Irvine Scientific Cat# 30093523 PEG 400 Sigma Aldrich Cat# PX1286B Manganese(II) chloride Sigma-Aldrich Cat# 244589 Phosphatase Inhibitor Cocktail (100X) Cell Signaling Technology Cat# 5870 SimplyBlue SafeStain Invitrogen Cat# LC6060 Y-27632 (Dihydrochloride) StemCell Technologies Cat# 100-1044 RPMI 1640 Medium Thermo Fisher Cat# 11875093 B-27 Supplement, minus insulin Thermo Fisher Cat# A1895601 B-27 Supplement Thermo Fisher Cat# 17504044 CHIR99021 StemCell Technologies Cat# 100-1042 Doxycycline Acros Organics Cat# 446060050 Lipofectamine RNAiMAX Thermo Fisher Cat# 13778150 L-Arginine-HCl Thermo Fisher Cat# 89989 L-Lysine-2HCl Thermo Fisher Cat# 89987 L-Arginine-HCl (13C6, 99%; 15N4, 99%) Cambridge Isotope Laboratories Cat# CNLM-539-H-0.05 L-Lysine-2HCl (13C6, 99%) Thermo Fisher Cat# 1860969 Fetal Bovine Serum, dialyzed Gibco Cat# A3382001 DMEM for SILAC Thermo Fisher Cat# 88364 Critical commercial assays PierceTM BCA Protein Assay Kit Thermo Fisher Cat# 23227 (Continued on next page) ll Cell 189, 1–18.e1–e16, July 23, 2026 e3 Article
Staining:Article Title: Laudanosine restricts Ebola virus entry by targeting TPC2-dependent endolysosomal trafficking
Article Snippet: .. The gels were running in 1x MES-SDS running buffer (20x MES-SDS running buffer diluted in deionized water) 30 min at 30 V and subsequently for 80 min at 90 V. Next, the separated proteins were wet blotted onto an Immun-Blot PVDF Membrane using the Thermo Fisher Mini Blot Module system (Cat# B1000) according to the manufacturers instruction and using the transfer buffer (25 mM Tris and 192 mM glycine at a pH of 8.3 with 20% (v/v) methanol) at a constant voltage of 20 V for 1 h. After blocking in Blocker Casein in PBS for 1 h, the proteins on the membrane were stained with primary antibodies diluted in TBS-T (1x TBS with 0.05% (v/v) Tween 20 and 0.1% (v/v) Blocker Casein in PBS) for 2 h at RT or overnight at 4 °C. ..
Article Title: Mitochondrial Lon protease couples substrate translocation to proteolytic activation
Article Snippet: .. Samples were collected at the indicated time points and mixed 1:1 with SDS loading buffer (0.13 M Tris-HCl, 0.2 M DTT, 15% glycerol, 0.02% bromophenol blue, 5% SDS, pH 6.8), followed by denaturation at 90 °C for 5 min. Proteins were separated on NuPAGETM 4-12% Bis-Tris Gel (Thermo Fisher Scientific) using NuPAGETM MES SDS Running Buffer (Thermo Fisher Scientific, product: NP0002) at 150 V for 60 min. Gels were stained with EZBlueTM Gel Staining Reagent (Sigma-Aldrich), and molecular weights were visualised using Precision Plus Protein Dual Colour Standards (Bio-Rad Laboratories, Inc.). ..
Article Title: mRNA 3' UTRs chaperone intrinsically disordered regions to control protein activity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Novex Tris-Glycine Mini Protein Gels, 6%, 1.0 mm, 12 well Invitrogen Cat# XP00062 NuPAGE MES SDS running buffer 20x Invitrogen Cat# NP0002 NuPAGE Tris-Acetate SDS running buffer 20x Invitrogen Cat# LA0041 NuPAGE Transfer Buffer (20X) Invitrogen Cat# NP00061 Novex Tris-Glycine SDS running buffer 10x Invitrogen Cat# LC2675 Odyssey blocking buffer (PBS) LI-COR Cat# 927-40000 Polybrene (hexadimethrine bromide) Sigma-Aldrich Cat# H9268 16% Paraformaldehde Aqueous Solution Fisher Scientific Cat# 50-980-487 Digitonin Sigma-Aldrich Cat# D141 SUPERase⋅InTM RNase Inhibitor Invitrogen Cat# AM2694 cOmplete EDTA-free Protease Inhibitor Cocktail Roche Cat# 04693159001 or Cat# 11836170001 PierceTM Anti-HA Magnetic Beads Thermo Fisher Cat# 88836 LIVE/DEADTM Fixable Near-IR Dead Cell Stain Kit Thermo Fisher Cat# L10119 LIVE/DEADTM Fixable Near IR (780) Viability Kit Thermo Fisher Cat# L34994 Puromycin Millipore Sigma Cat# 54-041-1 Cycloheximide Sigma-Aldrich Cat# C7698 Glycine Fisher Scientific Cat# BP381-1 D-Sucrose Fisher Scientific Cat# BP220-1 Ampicillin Sodium Salt Fisher Scientific Cat# BP176025 Kanamycin disulfate salt Sigma-Aldrich Cat# K1876 Sodium Chloride Fisher Scientific Cat# S271-3 Tris base Fisher Scientific Cat# BP152-1 Protein G Dynabeads Thermo Fisher Cat# 10004D KMet SNAP-ChIP Panel Epicypher Cat# 191100 Triton X-100 Fisher Scientific Cat# BP151-100 Tween-20 Fisher Scientific Cat# BP337-500 Na-deoxycholate Fisher Scientific Cat# BP349-100 SPRIselect magnetic beads Beckman Coulter Cat# B23318 Bovine Serum Albumin (BSA) Fisher Scientific Cat# BP1605100 Dextran Sulfate Sodium Salt Spectrum Chemical Cat# DE131 Ribonucleoside Vanadyl Complex NEB Cat# S1402 Salmon testes single stranded DNA Sigma-Aldrich Cat# D9156 Yeast tRNA Life Technologies Cat# 15401029 Formamide Millipore Sigma Cat# S4117 TURBO DNase Life Technologies Cat# AM2238 RNase I Life Technologies Cat# AM2295 Trichloroacetic Acid (TCA) TCI America Cat# T0369500G TALON Metal Affinity Resin Takara Cat# 635503 LB media Fisher Scientific Cat# BP1426-2 IPTG (Isopropyl-β-Dthiogalactopyranoside, dioxane free) RPI research Cat# I56000-25.0 PMSF (Benzylsulfonyl Fluoride) Sigma Aldrich Cat# 78830 Lysozyme Thermo Fisher Cat# 89833 DNase I Sigma-Aldrich Cat# 10104159001 Halt protease inhibitor cocktail Thermo Fisher Cat# 78439 (Continued on next page) ll e2 Cell 189, 1–18.e1–e16, July 23, 2026 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Imidazole Fisher Scientific Cat# O3196-500 Tris(2-carboxyethyl)phosphine hydrochloride (TCEP) Sigma-Aldrich Cat# C4706 Strep-Tactin® 4Flow® high-capacity resin Iba life sciences Cat# 2-1250-010 d-Desthiobiotin Sigma-Aldrich Cat# D1411 Cy5-UTP Enzo life sciences Cat# ENZ-42506 Streptavidin 680 LI-COR Cat# 926-68079 DSG Thermo Fisher Cat# 20593 BS3 (bis(sulfosuccinimidyl)suberate) Thermo Fisher Cat# 21580 ProLong Gold Antifade Mountant with DAPI DNA stain Thermo Fisher Cat# P36935 BMOE (bismaleimidoethane) Thermo Fisher Cat# 22323 Ethanol Fisher Scientific Cat# BP28184 DTT (dithiothreitol) Life Technologies Cat# R0862 Nonidet P-40 Sigma-Aldrich Cat# 74385 HEPES Sigma-Aldrich Cat# H4034 Magnesium Chloride Hexahydrate Fisher Scientific Cat# M33-500 Potassium Chloride Sigma-Aldrich Cat# P9541 Glycerol Sigma-Aldrich Cat# G5516 DMEM, free of phenol red and FBS Thermo Fisher Cat# 21063029 qScript cDNA SuperMix Quantabio Cat# 95048 PowerUp SYBRTM Green Master Mix Thermo Fisher Cat# A25742 4x LDS Sample buffer Thermo Fisher Cat# J61942.AD IRDye® 800CW DBCO Infrared Dye LI-COR Cat# 929-50000 5′ DNA Adenylation Kit NEB Cat# 2610 T4 RNA Ligase 1 (ssRNA Ligase) NEB Cat# M0204 T4 PNK NEB Cat# M0201 RNase A, DNase and protease-free (10 mg/ mL) Thermo Fisher Cat# EN0531 SureCastTM Acrylamide Solution (40%) Thermo Fisher Cat# HC2040 Phos-tag (TM) Acrylamide AAL-107 FUJIFILM Irvine Scientific Cat# 30093523 PEG 400 Sigma Aldrich Cat# PX1286B Manganese(II) chloride Sigma-Aldrich Cat# 244589 Phosphatase Inhibitor Cocktail (100X) Cell Signaling Technology Cat# 5870 SimplyBlue SafeStain Invitrogen Cat# LC6060 Y-27632 (Dihydrochloride) StemCell Technologies Cat# 100-1044 RPMI 1640 Medium Thermo Fisher Cat# 11875093 B-27 Supplement, minus insulin Thermo Fisher Cat# A1895601 B-27 Supplement Thermo Fisher Cat# 17504044 CHIR99021 StemCell Technologies Cat# 100-1042 Doxycycline Acros Organics Cat# 446060050 Lipofectamine RNAiMAX Thermo Fisher Cat# 13778150 L-Arginine-HCl Thermo Fisher Cat# 89989 L-Lysine-2HCl Thermo Fisher Cat# 89987 L-Arginine-HCl (13C6, 99%; 15N4, 99%) Cambridge Isotope Laboratories Cat# CNLM-539-H-0.05 L-Lysine-2HCl (13C6, 99%) Thermo Fisher Cat# 1860969 Fetal Bovine Serum, dialyzed Gibco Cat# A3382001 DMEM for SILAC Thermo Fisher Cat# 88364 Critical commercial assays PierceTM BCA Protein Assay Kit Thermo Fisher Cat# 23227 (Continued on next page) ll Cell 189, 1–18.e1–e16, July 23, 2026 e3 Article
Incubation:Article Title: Vacuole pH loss triggers ESCRT-dependent plasma membrane remodeling to prevent amino acid toxicity
Article Snippet: .. Samples were incubated at 50°C for 15 min, placed on ice for 30 s, and centrifuged for 3 min at 20,000 × g. Supernatants were resolved on Bolt 4–12% Bis-Tris Plus gels with MES/SDS buffer and transferred to nitrocellulose membranes with the Invitrogen Power Blotter Station. ..
SDS Page:Article Title: Anti-CD36 antibodies and their use to treat cancer
Article Snippet: .. Antibody purity was determined by SDS-PAGE (sodium dodecyl sulphate polyacrylamide gel electrophoresis) using an X-cell SureLock system with 4-12% Bis-Tris NuPAGE gels and NuPAGE MES buffer (Thermo). ..
Polyacrylamide Gel Electrophoresis:Article Title: Anti-CD36 antibodies and their use to treat cancer
Article Snippet: .. Antibody purity was determined by SDS-PAGE (sodium dodecyl sulphate polyacrylamide gel electrophoresis) using an X-cell SureLock system with 4-12% Bis-Tris NuPAGE gels and NuPAGE MES buffer (Thermo). ..
Protease Inhibitor:Article Title: mRNA 3' UTRs chaperone intrinsically disordered regions to control protein activity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Novex Tris-Glycine Mini Protein Gels, 6%, 1.0 mm, 12 well Invitrogen Cat# XP00062 NuPAGE MES SDS running buffer 20x Invitrogen Cat# NP0002 NuPAGE Tris-Acetate SDS running buffer 20x Invitrogen Cat# LA0041 NuPAGE Transfer Buffer (20X) Invitrogen Cat# NP00061 Novex Tris-Glycine SDS running buffer 10x Invitrogen Cat# LC2675 Odyssey blocking buffer (PBS) LI-COR Cat# 927-40000 Polybrene (hexadimethrine bromide) Sigma-Aldrich Cat# H9268 16% Paraformaldehde Aqueous Solution Fisher Scientific Cat# 50-980-487 Digitonin Sigma-Aldrich Cat# D141 SUPERase⋅InTM RNase Inhibitor Invitrogen Cat# AM2694 cOmplete EDTA-free Protease Inhibitor Cocktail Roche Cat# 04693159001 or Cat# 11836170001 PierceTM Anti-HA Magnetic Beads Thermo Fisher Cat# 88836 LIVE/DEADTM Fixable Near-IR Dead Cell Stain Kit Thermo Fisher Cat# L10119 LIVE/DEADTM Fixable Near IR (780) Viability Kit Thermo Fisher Cat# L34994 Puromycin Millipore Sigma Cat# 54-041-1 Cycloheximide Sigma-Aldrich Cat# C7698 Glycine Fisher Scientific Cat# BP381-1 D-Sucrose Fisher Scientific Cat# BP220-1 Ampicillin Sodium Salt Fisher Scientific Cat# BP176025 Kanamycin disulfate salt Sigma-Aldrich Cat# K1876 Sodium Chloride Fisher Scientific Cat# S271-3 Tris base Fisher Scientific Cat# BP152-1 Protein G Dynabeads Thermo Fisher Cat# 10004D KMet SNAP-ChIP Panel Epicypher Cat# 191100 Triton X-100 Fisher Scientific Cat# BP151-100 Tween-20 Fisher Scientific Cat# BP337-500 Na-deoxycholate Fisher Scientific Cat# BP349-100 SPRIselect magnetic beads Beckman Coulter Cat# B23318 Bovine Serum Albumin (BSA) Fisher Scientific Cat# BP1605100 Dextran Sulfate Sodium Salt Spectrum Chemical Cat# DE131 Ribonucleoside Vanadyl Complex NEB Cat# S1402 Salmon testes single stranded DNA Sigma-Aldrich Cat# D9156 Yeast tRNA Life Technologies Cat# 15401029 Formamide Millipore Sigma Cat# S4117 TURBO DNase Life Technologies Cat# AM2238 RNase I Life Technologies Cat# AM2295 Trichloroacetic Acid (TCA) TCI America Cat# T0369500G TALON Metal Affinity Resin Takara Cat# 635503 LB media Fisher Scientific Cat# BP1426-2 IPTG (Isopropyl-β-Dthiogalactopyranoside, dioxane free) RPI research Cat# I56000-25.0 PMSF (Benzylsulfonyl Fluoride) Sigma Aldrich Cat# 78830 Lysozyme Thermo Fisher Cat# 89833 DNase I Sigma-Aldrich Cat# 10104159001 Halt protease inhibitor cocktail Thermo Fisher Cat# 78439 (Continued on next page) ll e2 Cell 189, 1–18.e1–e16, July 23, 2026 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Imidazole Fisher Scientific Cat# O3196-500 Tris(2-carboxyethyl)phosphine hydrochloride (TCEP) Sigma-Aldrich Cat# C4706 Strep-Tactin® 4Flow® high-capacity resin Iba life sciences Cat# 2-1250-010 d-Desthiobiotin Sigma-Aldrich Cat# D1411 Cy5-UTP Enzo life sciences Cat# ENZ-42506 Streptavidin 680 LI-COR Cat# 926-68079 DSG Thermo Fisher Cat# 20593 BS3 (bis(sulfosuccinimidyl)suberate) Thermo Fisher Cat# 21580 ProLong Gold Antifade Mountant with DAPI DNA stain Thermo Fisher Cat# P36935 BMOE (bismaleimidoethane) Thermo Fisher Cat# 22323 Ethanol Fisher Scientific Cat# BP28184 DTT (dithiothreitol) Life Technologies Cat# R0862 Nonidet P-40 Sigma-Aldrich Cat# 74385 HEPES Sigma-Aldrich Cat# H4034 Magnesium Chloride Hexahydrate Fisher Scientific Cat# M33-500 Potassium Chloride Sigma-Aldrich Cat# P9541 Glycerol Sigma-Aldrich Cat# G5516 DMEM, free of phenol red and FBS Thermo Fisher Cat# 21063029 qScript cDNA SuperMix Quantabio Cat# 95048 PowerUp SYBRTM Green Master Mix Thermo Fisher Cat# A25742 4x LDS Sample buffer Thermo Fisher Cat# J61942.AD IRDye® 800CW DBCO Infrared Dye LI-COR Cat# 929-50000 5′ DNA Adenylation Kit NEB Cat# 2610 T4 RNA Ligase 1 (ssRNA Ligase) NEB Cat# M0204 T4 PNK NEB Cat# M0201 RNase A, DNase and protease-free (10 mg/ mL) Thermo Fisher Cat# EN0531 SureCastTM Acrylamide Solution (40%) Thermo Fisher Cat# HC2040 Phos-tag (TM) Acrylamide AAL-107 FUJIFILM Irvine Scientific Cat# 30093523 PEG 400 Sigma Aldrich Cat# PX1286B Manganese(II) chloride Sigma-Aldrich Cat# 244589 Phosphatase Inhibitor Cocktail (100X) Cell Signaling Technology Cat# 5870 SimplyBlue SafeStain Invitrogen Cat# LC6060 Y-27632 (Dihydrochloride) StemCell Technologies Cat# 100-1044 RPMI 1640 Medium Thermo Fisher Cat# 11875093 B-27 Supplement, minus insulin Thermo Fisher Cat# A1895601 B-27 Supplement Thermo Fisher Cat# 17504044 CHIR99021 StemCell Technologies Cat# 100-1042 Doxycycline Acros Organics Cat# 446060050 Lipofectamine RNAiMAX Thermo Fisher Cat# 13778150 L-Arginine-HCl Thermo Fisher Cat# 89989 L-Lysine-2HCl Thermo Fisher Cat# 89987 L-Arginine-HCl (13C6, 99%; 15N4, 99%) Cambridge Isotope Laboratories Cat# CNLM-539-H-0.05 L-Lysine-2HCl (13C6, 99%) Thermo Fisher Cat# 1860969 Fetal Bovine Serum, dialyzed Gibco Cat# A3382001 DMEM for SILAC Thermo Fisher Cat# 88364 Critical commercial assays PierceTM BCA Protein Assay Kit Thermo Fisher Cat# 23227 (Continued on next page) ll Cell 189, 1–18.e1–e16, July 23, 2026 e3 Article
Magnetic Beads:Article Title: mRNA 3' UTRs chaperone intrinsically disordered regions to control protein activity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Novex Tris-Glycine Mini Protein Gels, 6%, 1.0 mm, 12 well Invitrogen Cat# XP00062 NuPAGE MES SDS running buffer 20x Invitrogen Cat# NP0002 NuPAGE Tris-Acetate SDS running buffer 20x Invitrogen Cat# LA0041 NuPAGE Transfer Buffer (20X) Invitrogen Cat# NP00061 Novex Tris-Glycine SDS running buffer 10x Invitrogen Cat# LC2675 Odyssey blocking buffer (PBS) LI-COR Cat# 927-40000 Polybrene (hexadimethrine bromide) Sigma-Aldrich Cat# H9268 16% Paraformaldehde Aqueous Solution Fisher Scientific Cat# 50-980-487 Digitonin Sigma-Aldrich Cat# D141 SUPERase⋅InTM RNase Inhibitor Invitrogen Cat# AM2694 cOmplete EDTA-free Protease Inhibitor Cocktail Roche Cat# 04693159001 or Cat# 11836170001 PierceTM Anti-HA Magnetic Beads Thermo Fisher Cat# 88836 LIVE/DEADTM Fixable Near-IR Dead Cell Stain Kit Thermo Fisher Cat# L10119 LIVE/DEADTM Fixable Near IR (780) Viability Kit Thermo Fisher Cat# L34994 Puromycin Millipore Sigma Cat# 54-041-1 Cycloheximide Sigma-Aldrich Cat# C7698 Glycine Fisher Scientific Cat# BP381-1 D-Sucrose Fisher Scientific Cat# BP220-1 Ampicillin Sodium Salt Fisher Scientific Cat# BP176025 Kanamycin disulfate salt Sigma-Aldrich Cat# K1876 Sodium Chloride Fisher Scientific Cat# S271-3 Tris base Fisher Scientific Cat# BP152-1 Protein G Dynabeads Thermo Fisher Cat# 10004D KMet SNAP-ChIP Panel Epicypher Cat# 191100 Triton X-100 Fisher Scientific Cat# BP151-100 Tween-20 Fisher Scientific Cat# BP337-500 Na-deoxycholate Fisher Scientific Cat# BP349-100 SPRIselect magnetic beads Beckman Coulter Cat# B23318 Bovine Serum Albumin (BSA) Fisher Scientific Cat# BP1605100 Dextran Sulfate Sodium Salt Spectrum Chemical Cat# DE131 Ribonucleoside Vanadyl Complex NEB Cat# S1402 Salmon testes single stranded DNA Sigma-Aldrich Cat# D9156 Yeast tRNA Life Technologies Cat# 15401029 Formamide Millipore Sigma Cat# S4117 TURBO DNase Life Technologies Cat# AM2238 RNase I Life Technologies Cat# AM2295 Trichloroacetic Acid (TCA) TCI America Cat# T0369500G TALON Metal Affinity Resin Takara Cat# 635503 LB media Fisher Scientific Cat# BP1426-2 IPTG (Isopropyl-β-Dthiogalactopyranoside, dioxane free) RPI research Cat# I56000-25.0 PMSF (Benzylsulfonyl Fluoride) Sigma Aldrich Cat# 78830 Lysozyme Thermo Fisher Cat# 89833 DNase I Sigma-Aldrich Cat# 10104159001 Halt protease inhibitor cocktail Thermo Fisher Cat# 78439 (Continued on next page) ll e2 Cell 189, 1–18.e1–e16, July 23, 2026 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Imidazole Fisher Scientific Cat# O3196-500 Tris(2-carboxyethyl)phosphine hydrochloride (TCEP) Sigma-Aldrich Cat# C4706 Strep-Tactin® 4Flow® high-capacity resin Iba life sciences Cat# 2-1250-010 d-Desthiobiotin Sigma-Aldrich Cat# D1411 Cy5-UTP Enzo life sciences Cat# ENZ-42506 Streptavidin 680 LI-COR Cat# 926-68079 DSG Thermo Fisher Cat# 20593 BS3 (bis(sulfosuccinimidyl)suberate) Thermo Fisher Cat# 21580 ProLong Gold Antifade Mountant with DAPI DNA stain Thermo Fisher Cat# P36935 BMOE (bismaleimidoethane) Thermo Fisher Cat# 22323 Ethanol Fisher Scientific Cat# BP28184 DTT (dithiothreitol) Life Technologies Cat# R0862 Nonidet P-40 Sigma-Aldrich Cat# 74385 HEPES Sigma-Aldrich Cat# H4034 Magnesium Chloride Hexahydrate Fisher Scientific Cat# M33-500 Potassium Chloride Sigma-Aldrich Cat# P9541 Glycerol Sigma-Aldrich Cat# G5516 DMEM, free of phenol red and FBS Thermo Fisher Cat# 21063029 qScript cDNA SuperMix Quantabio Cat# 95048 PowerUp SYBRTM Green Master Mix Thermo Fisher Cat# A25742 4x LDS Sample buffer Thermo Fisher Cat# J61942.AD IRDye® 800CW DBCO Infrared Dye LI-COR Cat# 929-50000 5′ DNA Adenylation Kit NEB Cat# 2610 T4 RNA Ligase 1 (ssRNA Ligase) NEB Cat# M0204 T4 PNK NEB Cat# M0201 RNase A, DNase and protease-free (10 mg/ mL) Thermo Fisher Cat# EN0531 SureCastTM Acrylamide Solution (40%) Thermo Fisher Cat# HC2040 Phos-tag (TM) Acrylamide AAL-107 FUJIFILM Irvine Scientific Cat# 30093523 PEG 400 Sigma Aldrich Cat# PX1286B Manganese(II) chloride Sigma-Aldrich Cat# 244589 Phosphatase Inhibitor Cocktail (100X) Cell Signaling Technology Cat# 5870 SimplyBlue SafeStain Invitrogen Cat# LC6060 Y-27632 (Dihydrochloride) StemCell Technologies Cat# 100-1044 RPMI 1640 Medium Thermo Fisher Cat# 11875093 B-27 Supplement, minus insulin Thermo Fisher Cat# A1895601 B-27 Supplement Thermo Fisher Cat# 17504044 CHIR99021 StemCell Technologies Cat# 100-1042 Doxycycline Acros Organics Cat# 446060050 Lipofectamine RNAiMAX Thermo Fisher Cat# 13778150 L-Arginine-HCl Thermo Fisher Cat# 89989 L-Lysine-2HCl Thermo Fisher Cat# 89987 L-Arginine-HCl (13C6, 99%; 15N4, 99%) Cambridge Isotope Laboratories Cat# CNLM-539-H-0.05 L-Lysine-2HCl (13C6, 99%) Thermo Fisher Cat# 1860969 Fetal Bovine Serum, dialyzed Gibco Cat# A3382001 DMEM for SILAC Thermo Fisher Cat# 88364 Critical commercial assays PierceTM BCA Protein Assay Kit Thermo Fisher Cat# 23227 (Continued on next page) ll Cell 189, 1–18.e1–e16, July 23, 2026 e3 Article
Subcloning:Article Title: Methods of delivering anionic agents in vivo using non-viral nanoparticle-based delivery systems
Article Snippet: MTS cytotoxicity assay kit was obtained from Promega (Madison, WI, USA). .. Subcloning DH5-alpha competent cells and Lipofectamine 2000, Tracklt 10-bp DNA ladder (0.5 μg/μl), Pre-cast Nupage 4-12% Bis-Tris gels, Nupage MES running buffer, Nupage transfer buffer, Nupage LDS sample buffer (4×), nitrocellulose membrane 0.45 μm, blotting filter paper and magic mark (1 Kb) protein ladder were obtained from Invitrogen (Burlington, ON, Canada). .. Orange dye solution (6×) was obtained from Fermentas (Burlington, ON, Canada). pEGFPataxin1 (E3-82Q) plasmid was obtained from Dr. Zoghbi Huda from Baylor College of Medicine, Houston, USA.
|
Thermo Fisher is a verified supplier 
Thermo Fisher manufactures this product 