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automacs running buffer  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec automacs running buffer
    Automacs Running Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 383 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/running+buffer/autoMACS+Running+Buffer+-+MACS+Separation+Buffer/pmc03795073-113-6-10
    Average 97 stars, based on 383 article reviews
    automacs running buffer - by Bioz Stars, 2026-11
    97/100 stars

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    Related Articles

    Magnetic Cell Separation:

    Article Title: A protective hybrid protein vaccine informed by mapping major histocompatibility class II epitopes in Cryptococcus neoformans chitin deacetylases that stimulate CD4 + T-cell responses.
    Article Snippet: For the CD4-depleted group, splenocytes were depleted of CD4+ T cells using the Mouse CD4 (L3T4) MicroBeads (#130-117-043, Miltenyi Biotec, Bergisch Gladbach, Germany), a MACS LD column (#130-042-901, Miltenyi Biotec), and a QuadroMACS separator following the manufac turer’s instructions. .. Briefly, splenocytes (1 × 108 cells/mL in MACS Separation buffer (#130-091-221, Miltenyi Biotec) were mixed with CD4 (L3T4) MicroBeads (10% [vol/vol]) and incubated for 10 min at 4°C. ..

    Article Title: Donor-intrinsic regenerative potential outweighs histocompatibility in iPSC-derived skeletal muscle engraftment in nonhuman primates.
    Article Snippet: .. Hoechst 33342 Ready Flow Reagent Thermo Fisher Scientific Cat# R37165 Phytohemagglutinin (PHA) Thermo Fisher Scientific Cat# 00-4977-03 StemPro-34 medium Thermo Fisher Scientific Cat# 10639-011 Essential 8 medium Thermo Fisher Scientific Cat# A1517001 TrypLE Select Enzyme (1X), no phenol red Thermo Fisher Scientific Cat# 12563029 Ham’s F-10 Thermo Fisher Scientific Cat# 11550043 DMEM low glucose Thermo Fisher Scientific Cat# 11885084 DMEM high glucose Thermo Fisher Scientific Cat# 11965092 Penicillin-Streptomycin (5,000 U/mL) Thermo Fisher Scientific Cat# 15070063 Horse serum Thermo Fisher Scientific Cat# 26050088 IMDM Thermo Fisher Scientific Cat# 12440053 DMEM/F12 Thermo Fisher Scientific Cat# 11320033 KOSR Thermo Fisher Scientific Cat# 10828028 GlutaMAX Thermo Fisher Scientific Cat# 35050061 MACS buffer Miltenyi Biotec Cat# 130-091-221 Paraformaldehyde Millipore Sigma Cat# 1004965000 Triton X-100 Millipore Sigma Cat# T8787 Bovine Serum Albumin (BSA) Thermo Fisher Scientific Cat# BP1600-100 Lipofectamine LTX Reagent with PLUS Reagent Thermo Fisher Scientific Cat# 15338100 DAPI Santa Cruz Cat# sc-3598 .. CytoTune-iPS 2.0 Sendai Reprogramming Kit Thermo Fisher Scientific Cat# A16517 CFSE Cell Proliferation Kit Thermo Fisher Scientific Cat# C34554 PureLink RNA Mini Kit Thermo Fisher Scientific Cat# 12183018A Watchmaker Stranded mRNA Library Preparation Kit Watchmaker Genomics Cat# 7BK0001 SuperScript VILO cDNA Synthesis Kit Invitrogen Cat# 11754050 Premix Ex Taq (Probe qPCR) ROX Plus Takara Cat# RR39LR TaqMan assay Pax7 Thermo Fisher Scientific Hs00242962_m1 TaqMan assay TBP Thermo Fisher Scientific Mf00920495_m1

    Article Title: Lead Exposure During Differentiation Perturbs Electrophysiological Function in iPSC-Derived Cardiomyocytes in a Cell Line-Dependent Manner
    Article Snippet: First, cells were enzymatically dissociated with trypsin (#25200056, Thermo Fisher Scientific). .. After trypsin inactivation with EB20 media (reagents in below), cells were passed through 30 um pre-separation filters (#130-041-407 Miltenyi Biotec), centrifuged, washed with magnetic-activated cell sorting (MACS) buffer (#130-091-221, Miltenyi Biotec) centrifuged again, and then incubated in cardiomyocyte depletion primary antibody from the Miltenyi isolation kit for 10 minutes. ..

    Incubation:

    Article Title: A protective hybrid protein vaccine informed by mapping major histocompatibility class II epitopes in Cryptococcus neoformans chitin deacetylases that stimulate CD4 + T-cell responses.
    Article Snippet: For the CD4-depleted group, splenocytes were depleted of CD4+ T cells using the Mouse CD4 (L3T4) MicroBeads (#130-117-043, Miltenyi Biotec, Bergisch Gladbach, Germany), a MACS LD column (#130-042-901, Miltenyi Biotec), and a QuadroMACS separator following the manufac turer’s instructions. .. Briefly, splenocytes (1 × 108 cells/mL in MACS Separation buffer (#130-091-221, Miltenyi Biotec) were mixed with CD4 (L3T4) MicroBeads (10% [vol/vol]) and incubated for 10 min at 4°C. ..

    Article Title: Lead Exposure During Differentiation Perturbs Electrophysiological Function in iPSC-Derived Cardiomyocytes in a Cell Line-Dependent Manner
    Article Snippet: First, cells were enzymatically dissociated with trypsin (#25200056, Thermo Fisher Scientific). .. After trypsin inactivation with EB20 media (reagents in below), cells were passed through 30 um pre-separation filters (#130-041-407 Miltenyi Biotec), centrifuged, washed with magnetic-activated cell sorting (MACS) buffer (#130-091-221, Miltenyi Biotec) centrifuged again, and then incubated in cardiomyocyte depletion primary antibody from the Miltenyi isolation kit for 10 minutes. ..

    Flow Cytometry:

    Article Title: Donor-intrinsic regenerative potential outweighs histocompatibility in iPSC-derived skeletal muscle engraftment in nonhuman primates.
    Article Snippet: .. Hoechst 33342 Ready Flow Reagent Thermo Fisher Scientific Cat# R37165 Phytohemagglutinin (PHA) Thermo Fisher Scientific Cat# 00-4977-03 StemPro-34 medium Thermo Fisher Scientific Cat# 10639-011 Essential 8 medium Thermo Fisher Scientific Cat# A1517001 TrypLE Select Enzyme (1X), no phenol red Thermo Fisher Scientific Cat# 12563029 Ham’s F-10 Thermo Fisher Scientific Cat# 11550043 DMEM low glucose Thermo Fisher Scientific Cat# 11885084 DMEM high glucose Thermo Fisher Scientific Cat# 11965092 Penicillin-Streptomycin (5,000 U/mL) Thermo Fisher Scientific Cat# 15070063 Horse serum Thermo Fisher Scientific Cat# 26050088 IMDM Thermo Fisher Scientific Cat# 12440053 DMEM/F12 Thermo Fisher Scientific Cat# 11320033 KOSR Thermo Fisher Scientific Cat# 10828028 GlutaMAX Thermo Fisher Scientific Cat# 35050061 MACS buffer Miltenyi Biotec Cat# 130-091-221 Paraformaldehyde Millipore Sigma Cat# 1004965000 Triton X-100 Millipore Sigma Cat# T8787 Bovine Serum Albumin (BSA) Thermo Fisher Scientific Cat# BP1600-100 Lipofectamine LTX Reagent with PLUS Reagent Thermo Fisher Scientific Cat# 15338100 DAPI Santa Cruz Cat# sc-3598 .. CytoTune-iPS 2.0 Sendai Reprogramming Kit Thermo Fisher Scientific Cat# A16517 CFSE Cell Proliferation Kit Thermo Fisher Scientific Cat# C34554 PureLink RNA Mini Kit Thermo Fisher Scientific Cat# 12183018A Watchmaker Stranded mRNA Library Preparation Kit Watchmaker Genomics Cat# 7BK0001 SuperScript VILO cDNA Synthesis Kit Invitrogen Cat# 11754050 Premix Ex Taq (Probe qPCR) ROX Plus Takara Cat# RR39LR TaqMan assay Pax7 Thermo Fisher Scientific Hs00242962_m1 TaqMan assay TBP Thermo Fisher Scientific Mf00920495_m1

    Inactivation:

    Article Title: Lead Exposure During Differentiation Perturbs Electrophysiological Function in iPSC-Derived Cardiomyocytes in a Cell Line-Dependent Manner
    Article Snippet: First, cells were enzymatically dissociated with trypsin (#25200056, Thermo Fisher Scientific). .. After trypsin inactivation with EB20 media (reagents in below), cells were passed through 30 um pre-separation filters (#130-041-407 Miltenyi Biotec), centrifuged, washed with magnetic-activated cell sorting (MACS) buffer (#130-091-221, Miltenyi Biotec) centrifuged again, and then incubated in cardiomyocyte depletion primary antibody from the Miltenyi isolation kit for 10 minutes. ..

    FACS:

    Article Title: Lead Exposure During Differentiation Perturbs Electrophysiological Function in iPSC-Derived Cardiomyocytes in a Cell Line-Dependent Manner
    Article Snippet: First, cells were enzymatically dissociated with trypsin (#25200056, Thermo Fisher Scientific). .. After trypsin inactivation with EB20 media (reagents in below), cells were passed through 30 um pre-separation filters (#130-041-407 Miltenyi Biotec), centrifuged, washed with magnetic-activated cell sorting (MACS) buffer (#130-091-221, Miltenyi Biotec) centrifuged again, and then incubated in cardiomyocyte depletion primary antibody from the Miltenyi isolation kit for 10 minutes. ..

    Isolation:

    Article Title: Lead Exposure During Differentiation Perturbs Electrophysiological Function in iPSC-Derived Cardiomyocytes in a Cell Line-Dependent Manner
    Article Snippet: First, cells were enzymatically dissociated with trypsin (#25200056, Thermo Fisher Scientific). .. After trypsin inactivation with EB20 media (reagents in below), cells were passed through 30 um pre-separation filters (#130-041-407 Miltenyi Biotec), centrifuged, washed with magnetic-activated cell sorting (MACS) buffer (#130-091-221, Miltenyi Biotec) centrifuged again, and then incubated in cardiomyocyte depletion primary antibody from the Miltenyi isolation kit for 10 minutes. ..



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