other:Article Title: Aptamer-based nanoparticle drug delivery system designed for targeting GPC3-positive liver cancer
Article Snippet: Both the aptamers and proteins were dissolved in a running buffer composed of 25 mM HEPES, 0.4 M KCl, 10 mM MgCl2, and 0.005% Tween-20 (Sangon Biotech), adjusted to pH 7.4.
Virus:Article Title: The genetic repertoire of deep-sea microbiome: From sequence to structure and function.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains BL21(DE3) competent cell Vazyme Cat# C504-03 DH5α competent cell Sangon Biotech Cat# B528413-0100 Chemicals, peptides, and recombinant proteins 1M Tris-HCl solution, pH 7.5 Sangon Biotech Cat# B548124 5M sodium chloride solution Sigma-Aldrich Cat# S5150 10X TBE buffer Invitrogen Cat# AM9863 Nuclease-free water Invitrogen Cat# AM9932 HEPES Sigma-Aldrich Cat# 54457 PAGE Gels GenScript Cat# M01012C 20X MOPS/SDS running buffer Sangon Biotech Cat# C506051 30% Acr-Bis (29:1) Beyotime Cat# ST003 0.5M EDTA, pH 8.0 Invitrogen Cat# AM9261 SYBR safe DNA gel stain Invitrogen Cat# S33102 Yeast extract OXOID Cat# LP0021B Tryptone Sangon Biotech Cat# A505250 Imidazole Sangon Biotech Cat# A600277 Manganese(II) chloride tetrahydrate Sigma-Aldrich Cat# M5005 Prestained protein ladder Thermo Fisher Scientific Cat# 26616 Bovine thrombin Yeasen Biotechnology Cat# 20402ES05 BSA Sigma-Aldrish Cat# V900933 DTT Sangon Biotech Cat# A100281 PMSF Sangon Biotech Cat# A100754 Glycerol Sangon Biotech Cat# A100854 DMEM Gibco Cat# 10566016 FBS Gibco Cat# 10091148 DPBS Gibco Cat# 14190144 Tryple Gibco Cat# 12604013 50X TAE Buffer Sangon Biotech Cat# B548101 AO/PI Staining Solution Nexcelom Bioscience Cat# CS2-0106-5ML 10X Loading Buffer TaKaRa Cat# 9157 Acryl/Bis 30% Solution (37.5:1) Sangon Biotech Cat# B546018 10%APS Phygene Cat# PH1808 TEMED Sangon Biotech Cat# A610508 Glycogen, RNA grade Invitrogen Cat# R0551 Agarose, Regular Sangon Biotech Cat# A620014 Recombinant DsCas9 protein, full length, protein ID: DSM_0283490148 This paper N/A Recombinant DSH2 protein, full length, protein ID: DSM_0014922708 This paper N/A Recombinant DSH9 protein, full length, protein ID: DSM_0099674677 This paper N/A Recombinant DSH10 protein, full length, protein ID: DSM_0247050577 This paper N/A Critical commercial assays AMPure XP beads Beckman Coulter Agencourt Cat# B37419AA T4 DNA polymerase Enzymatics Cat# P708L (Continued on next page) Cell Host & Microbe 34, 1–14.e1–e10, August 12, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER TIANamp Genomic DNA kit TIANGEN Cat# DP304 PrimeSTAR® GXL Premix Fast, Dye plus TaKaRa Cat# R052B VAHTS DNA Clean Beads Vazyme Cat# N411-02 T7 Endonuclease I NEB Cat# M0302S Ultra Low Range DNA Ladder Invitrogen Cat# 10597012 NucleoSpin Gel and PCR Clean-up kit MACHERY-NAGEL Cat# 740609.25 T7 High Yield RNA Transcription Kit Vazyme Cat# TR101-01 Guide it IVT RNA Clean Up Kit TaKaRa Cat# 632638 EndoFree Mini Plasmid Kit TIANGEN Cat# DP123 RNeasy PowerFecal Pro Kit QIAGE Cat# 78404 MGIEasy Small RNA Library kit MGI Cat# 940-000196-00 Ni sepharose 6 fast flow Cytiva Cat# 17531804 Single-stranded DNA (ssDNA) cellulose Sigma-Aldrich Cat# D8273 Hitrap desalting columns Cytiva Cat# GE1714-08-01 HiTrap Heparin HP Cytiva Cat# 17-0407-03 HisSep Ni-NTA Agarose Resin Yeasen Biotechnology Cat# 20502ES60 Superdex 200 increase 10/300 GL Cytiva Cat# GE28-9909-44 Nanopore Gene Sequencer MGI Cat# H900-000005-00 DNBSEQ-T7 sequencer MGI Cat# H900-000129-00 CycloneSEQ Universal Library Prep Set MGI Cat# H940-000013 WT Sequencing Flow Cell MGI Cat# H930-000002-00 CycloneSEQ WT Sequencing Kit MGI Cat# H940-000016 Deposited data Raw data, see Table S1.
Recombinant:Article Title: The genetic repertoire of deep-sea microbiome: From sequence to structure and function.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains BL21(DE3) competent cell Vazyme Cat# C504-03 DH5α competent cell Sangon Biotech Cat# B528413-0100 Chemicals, peptides, and recombinant proteins 1M Tris-HCl solution, pH 7.5 Sangon Biotech Cat# B548124 5M sodium chloride solution Sigma-Aldrich Cat# S5150 10X TBE buffer Invitrogen Cat# AM9863 Nuclease-free water Invitrogen Cat# AM9932 HEPES Sigma-Aldrich Cat# 54457 PAGE Gels GenScript Cat# M01012C 20X MOPS/SDS running buffer Sangon Biotech Cat# C506051 30% Acr-Bis (29:1) Beyotime Cat# ST003 0.5M EDTA, pH 8.0 Invitrogen Cat# AM9261 SYBR safe DNA gel stain Invitrogen Cat# S33102 Yeast extract OXOID Cat# LP0021B Tryptone Sangon Biotech Cat# A505250 Imidazole Sangon Biotech Cat# A600277 Manganese(II) chloride tetrahydrate Sigma-Aldrich Cat# M5005 Prestained protein ladder Thermo Fisher Scientific Cat# 26616 Bovine thrombin Yeasen Biotechnology Cat# 20402ES05 BSA Sigma-Aldrish Cat# V900933 DTT Sangon Biotech Cat# A100281 PMSF Sangon Biotech Cat# A100754 Glycerol Sangon Biotech Cat# A100854 DMEM Gibco Cat# 10566016 FBS Gibco Cat# 10091148 DPBS Gibco Cat# 14190144 Tryple Gibco Cat# 12604013 50X TAE Buffer Sangon Biotech Cat# B548101 AO/PI Staining Solution Nexcelom Bioscience Cat# CS2-0106-5ML 10X Loading Buffer TaKaRa Cat# 9157 Acryl/Bis 30% Solution (37.5:1) Sangon Biotech Cat# B546018 10%APS Phygene Cat# PH1808 TEMED Sangon Biotech Cat# A610508 Glycogen, RNA grade Invitrogen Cat# R0551 Agarose, Regular Sangon Biotech Cat# A620014 Recombinant DsCas9 protein, full length, protein ID: DSM_0283490148 This paper N/A Recombinant DSH2 protein, full length, protein ID: DSM_0014922708 This paper N/A Recombinant DSH9 protein, full length, protein ID: DSM_0099674677 This paper N/A Recombinant DSH10 protein, full length, protein ID: DSM_0247050577 This paper N/A Critical commercial assays AMPure XP beads Beckman Coulter Agencourt Cat# B37419AA T4 DNA polymerase Enzymatics Cat# P708L (Continued on next page) Cell Host & Microbe 34, 1–14.e1–e10, August 12, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER TIANamp Genomic DNA kit TIANGEN Cat# DP304 PrimeSTAR® GXL Premix Fast, Dye plus TaKaRa Cat# R052B VAHTS DNA Clean Beads Vazyme Cat# N411-02 T7 Endonuclease I NEB Cat# M0302S Ultra Low Range DNA Ladder Invitrogen Cat# 10597012 NucleoSpin Gel and PCR Clean-up kit MACHERY-NAGEL Cat# 740609.25 T7 High Yield RNA Transcription Kit Vazyme Cat# TR101-01 Guide it IVT RNA Clean Up Kit TaKaRa Cat# 632638 EndoFree Mini Plasmid Kit TIANGEN Cat# DP123 RNeasy PowerFecal Pro Kit QIAGE Cat# 78404 MGIEasy Small RNA Library kit MGI Cat# 940-000196-00 Ni sepharose 6 fast flow Cytiva Cat# 17531804 Single-stranded DNA (ssDNA) cellulose Sigma-Aldrich Cat# D8273 Hitrap desalting columns Cytiva Cat# GE1714-08-01 HiTrap Heparin HP Cytiva Cat# 17-0407-03 HisSep Ni-NTA Agarose Resin Yeasen Biotechnology Cat# 20502ES60 Superdex 200 increase 10/300 GL Cytiva Cat# GE28-9909-44 Nanopore Gene Sequencer MGI Cat# H900-000005-00 DNBSEQ-T7 sequencer MGI Cat# H900-000129-00 CycloneSEQ Universal Library Prep Set MGI Cat# H940-000013 WT Sequencing Flow Cell MGI Cat# H930-000002-00 CycloneSEQ WT Sequencing Kit MGI Cat# H940-000016 Deposited data Raw data, see Table S1.
Polyacrylamide Gel Electrophoresis:Article Title: The genetic repertoire of deep-sea microbiome: From sequence to structure and function.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains BL21(DE3) competent cell Vazyme Cat# C504-03 DH5α competent cell Sangon Biotech Cat# B528413-0100 Chemicals, peptides, and recombinant proteins 1M Tris-HCl solution, pH 7.5 Sangon Biotech Cat# B548124 5M sodium chloride solution Sigma-Aldrich Cat# S5150 10X TBE buffer Invitrogen Cat# AM9863 Nuclease-free water Invitrogen Cat# AM9932 HEPES Sigma-Aldrich Cat# 54457 PAGE Gels GenScript Cat# M01012C 20X MOPS/SDS running buffer Sangon Biotech Cat# C506051 30% Acr-Bis (29:1) Beyotime Cat# ST003 0.5M EDTA, pH 8.0 Invitrogen Cat# AM9261 SYBR safe DNA gel stain Invitrogen Cat# S33102 Yeast extract OXOID Cat# LP0021B Tryptone Sangon Biotech Cat# A505250 Imidazole Sangon Biotech Cat# A600277 Manganese(II) chloride tetrahydrate Sigma-Aldrich Cat# M5005 Prestained protein ladder Thermo Fisher Scientific Cat# 26616 Bovine thrombin Yeasen Biotechnology Cat# 20402ES05 BSA Sigma-Aldrish Cat# V900933 DTT Sangon Biotech Cat# A100281 PMSF Sangon Biotech Cat# A100754 Glycerol Sangon Biotech Cat# A100854 DMEM Gibco Cat# 10566016 FBS Gibco Cat# 10091148 DPBS Gibco Cat# 14190144 Tryple Gibco Cat# 12604013 50X TAE Buffer Sangon Biotech Cat# B548101 AO/PI Staining Solution Nexcelom Bioscience Cat# CS2-0106-5ML 10X Loading Buffer TaKaRa Cat# 9157 Acryl/Bis 30% Solution (37.5:1) Sangon Biotech Cat# B546018 10%APS Phygene Cat# PH1808 TEMED Sangon Biotech Cat# A610508 Glycogen, RNA grade Invitrogen Cat# R0551 Agarose, Regular Sangon Biotech Cat# A620014 Recombinant DsCas9 protein, full length, protein ID: DSM_0283490148 This paper N/A Recombinant DSH2 protein, full length, protein ID: DSM_0014922708 This paper N/A Recombinant DSH9 protein, full length, protein ID: DSM_0099674677 This paper N/A Recombinant DSH10 protein, full length, protein ID: DSM_0247050577 This paper N/A Critical commercial assays AMPure XP beads Beckman Coulter Agencourt Cat# B37419AA T4 DNA polymerase Enzymatics Cat# P708L (Continued on next page) Cell Host & Microbe 34, 1–14.e1–e10, August 12, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER TIANamp Genomic DNA kit TIANGEN Cat# DP304 PrimeSTAR® GXL Premix Fast, Dye plus TaKaRa Cat# R052B VAHTS DNA Clean Beads Vazyme Cat# N411-02 T7 Endonuclease I NEB Cat# M0302S Ultra Low Range DNA Ladder Invitrogen Cat# 10597012 NucleoSpin Gel and PCR Clean-up kit MACHERY-NAGEL Cat# 740609.25 T7 High Yield RNA Transcription Kit Vazyme Cat# TR101-01 Guide it IVT RNA Clean Up Kit TaKaRa Cat# 632638 EndoFree Mini Plasmid Kit TIANGEN Cat# DP123 RNeasy PowerFecal Pro Kit QIAGE Cat# 78404 MGIEasy Small RNA Library kit MGI Cat# 940-000196-00 Ni sepharose 6 fast flow Cytiva Cat# 17531804 Single-stranded DNA (ssDNA) cellulose Sigma-Aldrich Cat# D8273 Hitrap desalting columns Cytiva Cat# GE1714-08-01 HiTrap Heparin HP Cytiva Cat# 17-0407-03 HisSep Ni-NTA Agarose Resin Yeasen Biotechnology Cat# 20502ES60 Superdex 200 increase 10/300 GL Cytiva Cat# GE28-9909-44 Nanopore Gene Sequencer MGI Cat# H900-000005-00 DNBSEQ-T7 sequencer MGI Cat# H900-000129-00 CycloneSEQ Universal Library Prep Set MGI Cat# H940-000013 WT Sequencing Flow Cell MGI Cat# H930-000002-00 CycloneSEQ WT Sequencing Kit MGI Cat# H940-000016 Deposited data Raw data, see Table S1.
Staining:Article Title: The genetic repertoire of deep-sea microbiome: From sequence to structure and function.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains BL21(DE3) competent cell Vazyme Cat# C504-03 DH5α competent cell Sangon Biotech Cat# B528413-0100 Chemicals, peptides, and recombinant proteins 1M Tris-HCl solution, pH 7.5 Sangon Biotech Cat# B548124 5M sodium chloride solution Sigma-Aldrich Cat# S5150 10X TBE buffer Invitrogen Cat# AM9863 Nuclease-free water Invitrogen Cat# AM9932 HEPES Sigma-Aldrich Cat# 54457 PAGE Gels GenScript Cat# M01012C 20X MOPS/SDS running buffer Sangon Biotech Cat# C506051 30% Acr-Bis (29:1) Beyotime Cat# ST003 0.5M EDTA, pH 8.0 Invitrogen Cat# AM9261 SYBR safe DNA gel stain Invitrogen Cat# S33102 Yeast extract OXOID Cat# LP0021B Tryptone Sangon Biotech Cat# A505250 Imidazole Sangon Biotech Cat# A600277 Manganese(II) chloride tetrahydrate Sigma-Aldrich Cat# M5005 Prestained protein ladder Thermo Fisher Scientific Cat# 26616 Bovine thrombin Yeasen Biotechnology Cat# 20402ES05 BSA Sigma-Aldrish Cat# V900933 DTT Sangon Biotech Cat# A100281 PMSF Sangon Biotech Cat# A100754 Glycerol Sangon Biotech Cat# A100854 DMEM Gibco Cat# 10566016 FBS Gibco Cat# 10091148 DPBS Gibco Cat# 14190144 Tryple Gibco Cat# 12604013 50X TAE Buffer Sangon Biotech Cat# B548101 AO/PI Staining Solution Nexcelom Bioscience Cat# CS2-0106-5ML 10X Loading Buffer TaKaRa Cat# 9157 Acryl/Bis 30% Solution (37.5:1) Sangon Biotech Cat# B546018 10%APS Phygene Cat# PH1808 TEMED Sangon Biotech Cat# A610508 Glycogen, RNA grade Invitrogen Cat# R0551 Agarose, Regular Sangon Biotech Cat# A620014 Recombinant DsCas9 protein, full length, protein ID: DSM_0283490148 This paper N/A Recombinant DSH2 protein, full length, protein ID: DSM_0014922708 This paper N/A Recombinant DSH9 protein, full length, protein ID: DSM_0099674677 This paper N/A Recombinant DSH10 protein, full length, protein ID: DSM_0247050577 This paper N/A Critical commercial assays AMPure XP beads Beckman Coulter Agencourt Cat# B37419AA T4 DNA polymerase Enzymatics Cat# P708L (Continued on next page) Cell Host & Microbe 34, 1–14.e1–e10, August 12, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER TIANamp Genomic DNA kit TIANGEN Cat# DP304 PrimeSTAR® GXL Premix Fast, Dye plus TaKaRa Cat# R052B VAHTS DNA Clean Beads Vazyme Cat# N411-02 T7 Endonuclease I NEB Cat# M0302S Ultra Low Range DNA Ladder Invitrogen Cat# 10597012 NucleoSpin Gel and PCR Clean-up kit MACHERY-NAGEL Cat# 740609.25 T7 High Yield RNA Transcription Kit Vazyme Cat# TR101-01 Guide it IVT RNA Clean Up Kit TaKaRa Cat# 632638 EndoFree Mini Plasmid Kit TIANGEN Cat# DP123 RNeasy PowerFecal Pro Kit QIAGE Cat# 78404 MGIEasy Small RNA Library kit MGI Cat# 940-000196-00 Ni sepharose 6 fast flow Cytiva Cat# 17531804 Single-stranded DNA (ssDNA) cellulose Sigma-Aldrich Cat# D8273 Hitrap desalting columns Cytiva Cat# GE1714-08-01 HiTrap Heparin HP Cytiva Cat# 17-0407-03 HisSep Ni-NTA Agarose Resin Yeasen Biotechnology Cat# 20502ES60 Superdex 200 increase 10/300 GL Cytiva Cat# GE28-9909-44 Nanopore Gene Sequencer MGI Cat# H900-000005-00 DNBSEQ-T7 sequencer MGI Cat# H900-000129-00 CycloneSEQ Universal Library Prep Set MGI Cat# H940-000013 WT Sequencing Flow Cell MGI Cat# H930-000002-00 CycloneSEQ WT Sequencing Kit MGI Cat# H940-000016 Deposited data Raw data, see Table S1.
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