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rtl-plus buffer
Rtl Plus Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rtl+plus+buffer/rtl+plus+buffer/pmc10998374-43-11-13
Average 90 stars, based on 1 article reviews
Rtl Plus Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rtl+plus+buffer/rtl+plus+buffer/pmc10998374-43-11-13
Average 90 stars, based on 1 article reviews
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2026-10
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Derivative Assay:Article Title: Multiomic QTL mapping reveals phenotypic complexity of GWAS loci and prioritizes putative causal variants. Article Snippet: Harvesting of material for molecular assays. (1) At D15, 107 PPCs derived from 106 iPSC clones from 106 individuals, pellets were collected and frozen in RTL plus buffer (Qiagen) for the RNA-seq assay. (2) At D15, 109 PPCs derived from 108 iPSC clones from 108 individuals were collected and frozen as nuclear pellets for the ATAC-seq assay. (3) H3K27ac ChIP-seq assay was not performed for the PPCs, however, we have frozen samples, and this dataset could be added in the future. .. Harvesting of material for molecular assays. (1) At D15, 107 PPCs derived from 106 iPSC clones from 106 individuals, pellets were collected and frozen in Article Title: Complex regulatory networks influence pluripotent cell state transitions in human iPSCs. Article Snippet: Emerging hiPSC colonies were manually picked after Day 21 and maintained on Matrigel (BD Corning) with mTeSR1 medium (Stem Cell Technologies). .. Multiple independently established hiPSC clones (i.e. referred to as lines) were derived from each individual, which were cultured typically to passage 12, and at least ten stock vials were frozen from each cell line. hiPSC pellets collected from each cell line were frozen in Article Title: Complex regulatory networks influence pluripotent cell state transitions in human iPSCs Article Snippet: Emerging hiPSC colonies were manually picked after Day 21 and maintained on Matrigel (BD Corning) with mTeSR1 medium (Stem Cell Technologies). .. Multiple independently established hiPSC clones (i.e. referred to as lines) were derived from each individual, which were cultured typically to passage 12, and at least ten stock vials were frozen from each cell line. hiPSC pellets collected from each cell line were frozen in Article Title: Multiomic QTL mapping reveals phenotypic complexity of GWAS loci and prioritizes putative causal variants Article Snippet: Stained cells were acquired using BD FACSCanto II system (BD Biosciences) and the fraction of cTnT-positive cells were calculated using FlowJo software version 10.2. .. Harvesting of material for molecular assays. (1) At D0 of the CVPC differentiation, 142 iPSC lines from 129 individuals were collected and frozen as nuclear pellets for the ATAC-seq assay∗∗. (2) At D0 of the CVPC differentiation, 43 iPSC lines from 41 individuals were collected and frozen as nuclear pellets for the H3K27ac ChIP-seq assay∗∗. (3) At D25, 178 CVPCs derived from 147 iPSC clones from 137 individuals, pellets were collected and frozen in Clone Assay:Article Title: Multiomic QTL mapping reveals phenotypic complexity of GWAS loci and prioritizes putative causal variants. Article Snippet: Harvesting of material for molecular assays. (1) At D15, 107 PPCs derived from 106 iPSC clones from 106 individuals, pellets were collected and frozen in RTL plus buffer (Qiagen) for the RNA-seq assay. (2) At D15, 109 PPCs derived from 108 iPSC clones from 108 individuals were collected and frozen as nuclear pellets for the ATAC-seq assay. (3) H3K27ac ChIP-seq assay was not performed for the PPCs, however, we have frozen samples, and this dataset could be added in the future. .. Harvesting of material for molecular assays. (1) At D15, 107 PPCs derived from 106 iPSC clones from 106 individuals, pellets were collected and frozen in Article Title: Complex regulatory networks influence pluripotent cell state transitions in human iPSCs. Article Snippet: Emerging hiPSC colonies were manually picked after Day 21 and maintained on Matrigel (BD Corning) with mTeSR1 medium (Stem Cell Technologies). .. Multiple independently established hiPSC clones (i.e. referred to as lines) were derived from each individual, which were cultured typically to passage 12, and at least ten stock vials were frozen from each cell line. hiPSC pellets collected from each cell line were frozen in Article Title: Complex regulatory networks influence pluripotent cell state transitions in human iPSCs Article Snippet: Emerging hiPSC colonies were manually picked after Day 21 and maintained on Matrigel (BD Corning) with mTeSR1 medium (Stem Cell Technologies). .. Multiple independently established hiPSC clones (i.e. referred to as lines) were derived from each individual, which were cultured typically to passage 12, and at least ten stock vials were frozen from each cell line. hiPSC pellets collected from each cell line were frozen in Article Title: Multiomic QTL mapping reveals phenotypic complexity of GWAS loci and prioritizes putative causal variants Article Snippet: Stained cells were acquired using BD FACSCanto II system (BD Biosciences) and the fraction of cTnT-positive cells were calculated using FlowJo software version 10.2. .. Harvesting of material for molecular assays. (1) At D0 of the CVPC differentiation, 142 iPSC lines from 129 individuals were collected and frozen as nuclear pellets for the ATAC-seq assay∗∗. (2) At D0 of the CVPC differentiation, 43 iPSC lines from 41 individuals were collected and frozen as nuclear pellets for the H3K27ac ChIP-seq assay∗∗. (3) At D25, 178 CVPCs derived from 147 iPSC clones from 137 individuals, pellets were collected and frozen in RNA Sequencing:Article Title: Multiomic QTL mapping reveals phenotypic complexity of GWAS loci and prioritizes putative causal variants. Article Snippet: Harvesting of material for molecular assays. (1) At D15, 107 PPCs derived from 106 iPSC clones from 106 individuals, pellets were collected and frozen in RTL plus buffer (Qiagen) for the RNA-seq assay. (2) At D15, 109 PPCs derived from 108 iPSC clones from 108 individuals were collected and frozen as nuclear pellets for the ATAC-seq assay. (3) H3K27ac ChIP-seq assay was not performed for the PPCs, however, we have frozen samples, and this dataset could be added in the future. .. Harvesting of material for molecular assays. (1) At D15, 107 PPCs derived from 106 iPSC clones from 106 individuals, pellets were collected and frozen in Article Title: Multiomic QTL mapping reveals phenotypic complexity of GWAS loci and prioritizes putative causal variants Article Snippet: Stained cells were acquired using BD FACSCanto II system (BD Biosciences) and the fraction of cTnT-positive cells were calculated using FlowJo software version 10.2. .. Harvesting of material for molecular assays. (1) At D0 of the CVPC differentiation, 142 iPSC lines from 129 individuals were collected and frozen as nuclear pellets for the ATAC-seq assay∗∗. (2) At D0 of the CVPC differentiation, 43 iPSC lines from 41 individuals were collected and frozen as nuclear pellets for the H3K27ac ChIP-seq assay∗∗. (3) At D25, 178 CVPCs derived from 147 iPSC clones from 137 individuals, pellets were collected and frozen in Article Title: Multiomic QTL mapping reveals phenotypic complexity of GWAS loci and prioritizes putative causal variants Article Snippet: .. Harvesting of material for molecular assays. (1) At P12, for 220 iPSC lines from 220 individuals, pellets were collected and frozen in Chromatin Immunoprecipitation:Article Title: Multiomic QTL mapping reveals phenotypic complexity of GWAS loci and prioritizes putative causal variants. Article Snippet: Harvesting of material for molecular assays. (1) At D15, 107 PPCs derived from 106 iPSC clones from 106 individuals, pellets were collected and frozen in RTL plus buffer (Qiagen) for the RNA-seq assay. (2) At D15, 109 PPCs derived from 108 iPSC clones from 108 individuals were collected and frozen as nuclear pellets for the ATAC-seq assay. (3) H3K27ac ChIP-seq assay was not performed for the PPCs, however, we have frozen samples, and this dataset could be added in the future. .. Harvesting of material for molecular assays. (1) At D15, 107 PPCs derived from 106 iPSC clones from 106 individuals, pellets were collected and frozen in Article Title: Multiomic QTL mapping reveals phenotypic complexity of GWAS loci and prioritizes putative causal variants Article Snippet: Stained cells were acquired using BD FACSCanto II system (BD Biosciences) and the fraction of cTnT-positive cells were calculated using FlowJo software version 10.2. .. Harvesting of material for molecular assays. (1) At D0 of the CVPC differentiation, 142 iPSC lines from 129 individuals were collected and frozen as nuclear pellets for the ATAC-seq assay∗∗. (2) At D0 of the CVPC differentiation, 43 iPSC lines from 41 individuals were collected and frozen as nuclear pellets for the H3K27ac ChIP-seq assay∗∗. (3) At D25, 178 CVPCs derived from 147 iPSC clones from 137 individuals, pellets were collected and frozen in Article Title: Multiomic QTL mapping reveals phenotypic complexity of GWAS loci and prioritizes putative causal variants Article Snippet: .. Harvesting of material for molecular assays. (1) At P12, for 220 iPSC lines from 220 individuals, pellets were collected and frozen in Cell Culture:Article Title: Complex regulatory networks influence pluripotent cell state transitions in human iPSCs. Article Snippet: Emerging hiPSC colonies were manually picked after Day 21 and maintained on Matrigel (BD Corning) with mTeSR1 medium (Stem Cell Technologies). .. Multiple independently established hiPSC clones (i.e. referred to as lines) were derived from each individual, which were cultured typically to passage 12, and at least ten stock vials were frozen from each cell line. hiPSC pellets collected from each cell line were frozen in Article Title: Complex regulatory networks influence pluripotent cell state transitions in human iPSCs Article Snippet: Emerging hiPSC colonies were manually picked after Day 21 and maintained on Matrigel (BD Corning) with mTeSR1 medium (Stem Cell Technologies). .. Multiple independently established hiPSC clones (i.e. referred to as lines) were derived from each individual, which were cultured typically to passage 12, and at least ten stock vials were frozen from each cell line. hiPSC pellets collected from each cell line were frozen in Isolation:Article Title: Complex regulatory networks influence pluripotent cell state transitions in human iPSCs. Article Snippet: Emerging hiPSC colonies were manually picked after Day 21 and maintained on Matrigel (BD Corning) with mTeSR1 medium (Stem Cell Technologies). .. Multiple independently established hiPSC clones (i.e. referred to as lines) were derived from each individual, which were cultured typically to passage 12, and at least ten stock vials were frozen from each cell line. hiPSC pellets collected from each cell line were frozen in Article Title: Complex regulatory networks influence pluripotent cell state transitions in human iPSCs Article Snippet: Emerging hiPSC colonies were manually picked after Day 21 and maintained on Matrigel (BD Corning) with mTeSR1 medium (Stem Cell Technologies). .. Multiple independently established hiPSC clones (i.e. referred to as lines) were derived from each individual, which were cultured typically to passage 12, and at least ten stock vials were frozen from each cell line. hiPSC pellets collected from each cell line were frozen in other:Article Title: Multiomic QTL mapping reveals phenotypic complexity of GWAS loci and prioritizes putative causal variants. Article Snippet: CVPC differentiation As previously described in detail,90 to generate CVPCs, we used a small molecule cardiac differentiation protocol.91 The 25-day differentiation protocol consisted of four phases. (1) Expansion of iPSC: One vial of each iPSC line was thawed intomTeSR1medium containing 10 mMROCK Inhibitor (Sigma) and plated on one well of a 6-well plate coated overnight with matrigel. Article Title: Single-cell profiling reveals transcriptome dynamics during bovine oocyte growth Article Snippet: Measured oocytes were snap-frozen in 4 μL PBS + 4 μL |