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Promega rt buffer
Rt Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rt+buffer+promega/rt+buffer/pm16105095-99-31-34
Average 90 stars, based on 1 article reviews

rt buffer - by Bioz Stars, 2026-10

90/100 stars

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Reverse Transcription:

Article Title: Integrated workflow for discovery of microprotein-coding small open reading frames.
Article Snippet: .. Reverse Transcription mix Reagent Amount Promega RNasin Plus inhibitor 0.5 mL 10 mM dNTP mix 1 mL 103 Reverse Transcription (RT) buffer 3 mL 100 mM DTT 1.5 mL 5 mM RT primer 1 mL EpiScript Reverse Transcriptase 1 mL Nuclease-free water 5 mL Total Volume 13 mL 12 STAR Protocols 4, 102649, December 15, 2023 f. Elute cDNA samples with 11 mL nuclease-free water and keep the samples on ice or store at them 20 C. Expect to recover 10 mL. ..

Article Title: Successful establishment of primary small airway cell cultures in human lung transplantation
Article Snippet: .. Gene expression was analyzed using two-step reverse transcription polymerase chain reaction (RT-PCR) and cDNA synthesized using hexanucleotide primers and MultiscribeTM Reverse Transcriptase (Applied Biosystems, Foster City, USA) in a final reaction volume of 20 μL containing 1 × RT buffer (Promega Madison, USA), 5.5 mM MgCl 2 , 0.5 mM of each of the dNTPs, 2.5 μM random hexamers, 0.4 U RNase inhibitor, 1.25 U Multiscribe (Applied Biosystems, Foster City, USA) reverse transcriptase and 200 ng RNA. ..

Article Title: Anticancer actions of carnosine in cellular models of prostate cancer
Article Snippet: Total RNA was extracted using the RNeasy Mini Extraction Kit (QIAGEN) according to the manufacturer's protocol and the concentration of extracted RNA was measured on a NanoDrop spectrophotometer (Thermo Fisher). .. To synthesize cDNA, 2 μg of RNA in nuclease‐free water (final volume 9 μL) and 1 μL of oligo dT (Promega) were heated at 70°C for 5 min. A mix containing 5 μL of RT buffer (Promega), 1 μL of Reverse Transcriptase enzyme (Promega), 0.7 μL of RNasin (Promega), 1 μL of dNTPs (Promega) and 7.3 μL of nuclease‐free water were then added to the reaction and incubated at 40°C for 1 h. The reaction was heated at 95°C for 5 min and frozen immediately at −20°C. .. The RT‐qPCR reaction was performed in a final volume of 13 μL by mixing 1 μL of cDNA, 6.25 μL of iTaq Universal SYBR Green supermix (Bio‐Rad), 0.5 μL of forward and reverse primer (10 pM) and 4.75 μL of nuclease‐free water.

Article Title: A Possible Novel Anti-Inflammatory Mechanism for the Pharmacological Prolyl Hydroxylase Inhibitor 3,4-Dihydroxybenzoate: Implications for Use as a Therapeutic for Parkinson's Disease
Article Snippet: .. Total RNA (1 μ g) was then reverse-transcribed in a reaction mixture containing 1 U RNase inhibitor, 500 ng random primers, 3 mM MgCl 2 , 0.5 mM dNTP, and 10 U reverse transcriptase in RT buffer (Promega). .. The synthesized cDNA was used as a template for qPCR analysis using the universal probe library system from Roche.

Article Title: Anticancer actions of carnosine in cellular models of prostate cancer.
Article Snippet: Total RNA was extracted using the RNeasy Mini Extraction Kit (QIAGEN) according to the manufacturer's protocol and the concentration of extracted RNA was measured on a NanoDrop spectrophotometer (Thermo Fisher). .. To synthesize cDNA, 2 μg of RNA in nuclease-free water (final volume 9 μL) and 1 μL of oligo dT (Promega) were heated at 70°C for 5 min. A mix containing 5 μL of RT buffer (Promega), 1 μL of Reverse Transcriptase enzyme (Promega), 0.7 μL of RNasin (Promega), 1 μL of dNTPs (Promega) and 7.3 μL of nuclease-free water were then added to the reaction and incubated at 40°C for 1 h. The reaction was heated at 95°C for 5 min and frozen immediately at −20°C. .. The RT-qPCR reaction was performed in a final volume of 13 μL by mixing 1 μL of cDNA, 6.25 μL of iTaq Universal SYBR Green supermix (Bio-Rad), 0.5 μL of forward and reverse primer (10 pM) and 4.75 μL of nuclease-free water.

other:

Article Title: Antiviral action of a functionalized plastic surface against human coronaviruses.
Article Snippet: Processing of the flushed samples to measure the viral RNA was done through the following steps: (i) the samples were diluted five times with RNase-free water (J71768, Thermo Fisher Scientific, Geel, Belgium) and heat treated for 5 min at 75°C to release the RNA from the viruses. (ii) Reverse transcription (RT’) was carried out to make cDNA from the viral RNA using 20U M-MLV reverse transcriptase enzyme (Promega, WI, USA), RT-buffer (Promega, WI, USA), RNase-free water (J71768, Thermo Fisher Scientific), 4U RNAsin ribonuclease inhibitor (Promega, WI, USA), 0.5 mM dNTPs (Promega, WI, USA), and 1.2 μM reverse primer (5′-AATGTAAAGATGRCCGCGTATT) (Merck, Darmstadt, Germany).

Article Title: Antiviral action of a functionalized plastic surface against human coronaviruses
Article Snippet: Processing of the flushed samples to measure the viral RNA was done through the following steps: (i) the samples were diluted five times with RNase-free water (J71768, Thermo Fisher Scientific, Geel, Belgium) and heat treated for 5 min at 75°C to release the RNA from the viruses. (ii) Reverse transcription (RT’) was carried out to make cDNA from the viral RNA using 20U M-MLV reverse transcriptase enzyme (Promega, WI, USA), RT-buffer (Promega, WI, USA), RNase-free water (J71768, Thermo Fisher Scientific), 4U RNAsin ribonuclease inhibitor (Promega, WI, USA), 0.5 mM dNTPs (Promega, WI, USA), and 1.2 μM reverse primer (5′- AATGTAAAGATG RCCGCGTATT) (Merck, Darmstadt, Germany).

Gene Expression:

Polymerase Chain Reaction:

Synthesized:

Incubation:

Article Title: Dual natural infection with bovine viral diarrhea virus -1 and -2 in a stillborn calf: tissue distribution and molecular characterization
Article Snippet: .. Thereafter, 5 μl of RT Buffer, 1 μl of dNTPs (10 mM each, Promega, USA), 1 μl (200U) of M-MLV (Promega, USA), and 3 μl of dH 2 O was incubated at 37oC for 50 min and 70oC for 15 min. .. The first round of PCR was conducted in a total volume of 50 μl containing 2.5 μl of cDNA, 5 μl of MgCl 2 (25mM), 10 μl of 5X GoTaq ® Buffer, 1 μl of dNTPs (10 mM each), 1.5 μl of each external primer (10 μM), 0.5 μl of GoTaq ® DNA Polymerase (Promega, USA) and 28 μl of dH 2 O. Cycling conditions were as follows: 1 min 94oC; 25 cycles of 1 min 94oC, 1 min 50oC, 2 min 72oC; and a final step of 15 min 72oC.

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