5× rt buffer (Promega)
90
Structured Review
Promega
5× rt buffer
5× Rt Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rt+buffer+promega/5%C3%97+rt+buffer/pmc03467402-48-5-7
Average 90 stars, based on 1 article reviews
5× Rt Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rt+buffer+promega/5%C3%97+rt+buffer/pmc03467402-48-5-7
Average 90 stars, based on 1 article reviews
5× rt buffer - by Bioz Stars,
2026-10
90/100 stars
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cDNA Synthesis:Article Title: Convergent evolution of water-conducting cells in Marchantia recruited the ZHOUPI gene promoting cell wall reinforcement and programmed cell death. Article Snippet: The RNA was eluted from the columns with 40 ml RNAse-free water and its concentration was estimated by measuring the absorbance at 260 nm using a 2 ml aliquot in a Nanodrop spectrophotometer. .. For cDNA synthesis 1 ml anchored oligo-dT primer (1 mg/ml) and 1 mg total RNA were combined in a 10 ml volume and incubated at 65oC for 5 minutes to denature secondary structure then cooled rapidly on ice for 1 min. A 20 ml reverse transcriptase (RT) reaction was assembled with 1 ml 10 mM deoxyribonucleotide triphosphates (dNTP), 4 ml Incubation:Article Title: Convergent evolution of water-conducting cells in Marchantia recruited the ZHOUPI gene promoting cell wall reinforcement and programmed cell death. Article Snippet: The RNA was eluted from the columns with 40 ml RNAse-free water and its concentration was estimated by measuring the absorbance at 260 nm using a 2 ml aliquot in a Nanodrop spectrophotometer. .. For cDNA synthesis 1 ml anchored oligo-dT primer (1 mg/ml) and 1 mg total RNA were combined in a 10 ml volume and incubated at 65oC for 5 minutes to denature secondary structure then cooled rapidly on ice for 1 min. A 20 ml reverse transcriptase (RT) reaction was assembled with 1 ml 10 mM deoxyribonucleotide triphosphates (dNTP), 4 ml Reverse Transcription:Article Title: Convergent evolution of water-conducting cells in Marchantia recruited the ZHOUPI gene promoting cell wall reinforcement and programmed cell death. Article Snippet: The RNA was eluted from the columns with 40 ml RNAse-free water and its concentration was estimated by measuring the absorbance at 260 nm using a 2 ml aliquot in a Nanodrop spectrophotometer. .. For cDNA synthesis 1 ml anchored oligo-dT primer (1 mg/ml) and 1 mg total RNA were combined in a 10 ml volume and incubated at 65oC for 5 minutes to denature secondary structure then cooled rapidly on ice for 1 min. A 20 ml reverse transcriptase (RT) reaction was assembled with 1 ml 10 mM deoxyribonucleotide triphosphates (dNTP), 4 ml Article Title: RFX6 haploinsufficiency predisposes to diabetes through impaired beta cell functionality Article Snippet: Total RNA was extracted using NucleoSpin Plus RNA kit (Macherey-Nagel). .. A total of 1.5 μg RNA was reversely transcribed using 0.5 μl Moloney murine leukemia virus reverse transcriptase (M1701, Promega), 4 μl other:Article Title: High genetic diversity suggests a long evolutionary history of plum pox <scp>virus‐M</scp> in Turkey Article Snippet: Plum pox virus (PPV), a member of the genus Potyvirus, is the causal agent of sharka disease, one of the important diseases of stone fruit, particularly apricots (Prunus armeniaca and P. mume), peach (P. persica) and plums (P. domestica and P. cerasifera) (Cambra et al., 2006; García et al., 2014; Maejima et al., 2011).. The disease may result in the formation of twisted or distorted leaves, yellow veins, necrotic or chlorotic ring pattern on leaves and discoloured rings and deformations on fruits.. In severe cases, early fruit drop and reduced yield are also observed. Article Title: SERCA2a, Phospholamban, Sarcolipin, and Ryanodine Receptors Gene Expression in Children with Congenital Heart Defects Article Snippet: RNA extracted (400 ng) was then added to a mixture containing 250 ng of random examers (Gibco BRL Life Technologies, MD, USA), 2 μL of 5× Article Title: MicroRNA-141-regulated KLK10 and TNFSF-15 gene expression in hepatoblastoma cells as a novel mechanism in liver carcinogenesis. Article Snippet: The extracted total RNA was then purified using the RNeasy Mini Kit (Qiagen, USA), followed by the purification of small miRNAs using the RNeasy MinElute Cleanup Kit (Qiagen, USA) according to the manufacturer’s protocol. cRNA was synthesized from total RNA using miR-141-Pst1-Reverse primer and the following reagents: 4 μL 10× Article Title: Pravastatin Protects Cytotrophoblasts from Hyperglycemia-Induced Preeclampsia Phenotype Article Snippet: Subsequently, 2 μL of 10× RT Buffer, 4 μL of dNTP Mix, 1 μL of RNase Inhibitor, and 1 μL of Article Title: Reemergence of dengue virus type-3 (subtype-III) in India: Implications for increased incidence of DHF & DSS Article Snippet: The complementary DNA (cDNA) was synthesized in a 10 μl reaction volume with Virus:Article Title: RFX6 haploinsufficiency predisposes to diabetes through impaired beta cell functionality Article Snippet: Total RNA was extracted using NucleoSpin Plus RNA kit (Macherey-Nagel). .. A total of 1.5 μg RNA was reversely transcribed using 0.5 μl Moloney murine leukemia virus reverse transcriptase (M1701, Promega), 4 μl |
