one step rt qpcr toughmix (Quanta Biosciences)
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one step rt qpcr toughmix
One Step Rt Qpcr Toughmix, supplied by Quanta Biosciences, used in various techniques. Bioz Stars score: 96/100, based on 503 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rt+1/qScript+XLT+1-Step+RT-qPCR+ToughMix/pmc13124052-159-38-41
Average 96 stars, based on 503 article reviews
One Step Rt Qpcr Toughmix, supplied by Quanta Biosciences, used in various techniques. Bioz Stars score: 96/100, based on 503 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rt+1/qScript+XLT+1-Step+RT-qPCR+ToughMix/pmc13124052-159-38-41
Average 96 stars, based on 503 article reviews
one step rt qpcr toughmix - by Bioz Stars,
2026-11
96/100 stars
Images
1) Product Images from "Translational repression of viral RNAs supports persistent arbovirus infection in mosquitoes"
Article Title: Translational repression of viral RNAs supports persistent arbovirus infection in mosquitoes
Journal: PLOS Biology
doi: 10.1371/journal.pbio.3003702
Figure Legend Snippet: (A) Schematic representation of CHIKV RNA genome organization and viral protein expression strategy. Created in BioRender. Diez, J. (2026) https://BioRender.com/8srpphg . (B) CHIKV titers over time in U4.4 and C6/36, determined by plaque assay. (C) Kinetics of CHIKV gRNA and sgRNA levels in U4.4 and C6/36, quantified by RT-qPCR. (D and E) Expression of CHIKV nsP1 and capsid ( C ) proteins in U4.4 (D) and C6/36 (E) cells over the course of infection. Green and blue colors correspond to U4.4 and C6/36 cells, respectively. All infections were performed at an MOI of 4 and samples were collected at the indicated time post infection. Data points represent the mean ± SEM of 3 independent replicates. Viral RNA levels were quantified using a standard curve generated from in vitro -transcribed CHIKV RNA. Protein expression levels correspond to band intensities normalized to tubulin (Tub) and to the 1 dpi value within each blot. Dotted lines indicate separated lanes in the same membrane. All underlying data can be found in and in .
Techniques Used: Expressing, Plaque Assay, Quantitative RT-PCR, Infection, Generated, In Vitro, Membrane
Figure Legend Snippet: (A and B) Representative polysome profile analyses of U4.4 cells (A) and of C6/36 cells (B) at the indicated time points after CHIKV infection. (C and D) Polysome-to-monosome (P/M) ratios reflecting global mRNA translation in U4.4 and C6/36 cells over the course of infection calculated from the area under-the-curve (AUC) of polysome profiles and normalized to noninfected controls and to 1 dpi. (E) Levels of phosphorylated eIF2α in U4.4 and C6/36 cells during CHIKV infection were assessed by western blotting. Protein expression levels were normalized to tubulin (Tub) and to the noninfected value within each blot. Dotted lines indicate separated lanes in the same membrane. (F) P/M ratios of viral CHIKV gRNA and sgRNA in U4.4 and C6/36 cells over the course of infection were determined by RT-qPCR of RNA extracted from polysome fractions. Values are expressed relative to 1 dpi. Bars represent the mean ± SEM of 3 independent replicates. All underlying data can be found in and in .
Techniques Used: Infection, Western Blot, Expressing, Membrane, Quantitative RT-PCR
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RNA was extracted from cell lysate derived from ChIRP-MS samples using TRizol (Invitrogen, Waltham, USA) and eluted in 30 μl of RNAse-free H 2 O (Qiagen, Venlo, Netherlands) For quantifying TBEV RNA, quantitative reverse transcriptase PCR (RT-qPCR) was performed on 2 μl of extracted RNA using primers specific for the envelope gene (E) of TBEV Hypr (5′ TGT TTC CAT GGC AGA GCC AG and 5′ TCC TTG AGC TTG ACA AGA CAG) and probe (5′ 6-carboxyfluorescein [FAM]- T GGA ACA CCT TCC AAC GGC TTG GCA-minor groove binder nonfluorescent quencher [MGB-NFQ]) (Eurobio, Les Ulis, France) and the Quantitative RT-PCR:Article Title: An RNA-centric interactomics screen identifies novel proviral and antiviral genome binding interactors for tick-borne encephalitis virus Article Snippet: .. 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SARS-CoV-2 detection as performed using the Article Title: Zika virus serologic responses during pregnancy in a dengue-endemic setting: A prospective cohort study in Thailand. Article Snippet: .. Participants with ZIKV-reactive serologic responses or presentation of symptoms meeting the febrile surveillance criteria (Fig 1) underwent RT-qPCR testing targeting a conserved region of the E gene, performed using Article Title: DNA-dependent RNA polymerase incorporates β-D-N4-hydroxycytidine (NHC) linking Molnupiravir to host transcription-dependent mutagenesis Article Snippet: Then, it is treated with TURBO DNA-free Kit (Invitrogen, ThermoFisher Scientific, Cat. AM1907), 100 μl (200 units) of DNAse for 250 μl reaction. .. 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Copy numbers of the DNA template in IVT reactions were quantified by qPCR with LightCycler 480 Probes Master (Roche), and the copy numbers of the RNA products in the IVT reactions were determined with the qScript XLT other:Article Title: DNA-dependent RNA polymerase incorporates β-D-N4-hydroxycytidine (NHC) linking Molnupiravir to host transcription-dependent mutagenesis Article Snippet: Then, they were treated with TURBO DNA-free Kit (Invitrogen, ThermoFisher Scientific, Cat. AM1907); 1 μl of DNAse in 50 μl reaction and incubated at 37 °C for 1 h according to the manufacturer’s protocol. |
