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rox reference dye  (tiangen biotech co)


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    Structured Review

    tiangen biotech co rox reference dye
    Rox Reference Dye, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 99/100, based on 362 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rox+reference+dye/Reverse+Primer/pm41365136-82-16-19
    Average 99 stars, based on 362 article reviews
    rox reference dye - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: The protective role of a moderate protein diet in ETEC-infected piglets: Optimization of growth, immunity, and microbial balance
    Article Snippet: Total bacteria were identified in a final volume of 25 μL with 1 μL forward primer, 1 μL reverse primer, 12.5 μL TB Green Premix EX Taq II Fast (TaKaRa Biotechnology Co., Ltd., Dalian, Liaoning, China), 1 μL template DNA, and 9.5 μL nuclease-free water. .. The thermal cycling parameters were as follows: pre-denaturation step at 95 °C for 30 s and 40 cycles of 95 °C for 5 s, 60 °C for 30 s. For the quantification of Lactobacillus , E . coli , Bacillus , and Bifidobacterium , real-time quantificative PCR was conducted in a 20 μL volume with 0.4 μL probe enhancer solution, 0.2 μL ROX Reference Dcye, 0.6 μL forward and 0.6 μL reverse primer, 10 μL SuperReal Premix (Tiangen Biotech Co., Ltd., Beijing, China), 1 μL template DNA, and 7.2 μL nuclease-free water. ..

    Article Title: Five New Species of Gibellula (Hypocreales, Cordycipitaceae) from China.
    Article Snippet: .. The PCR mixture (25 μL) consisted of 1 μL of DNA template, 1 μL of each forward and reverse primer (10 μM each), 9.5 μL of ddH2O, and 12.5 μL of 2× Taq PCR Master Mix (TIANGEN, Beijing, China). ..

    Article Title: The protective role of a moderate protein diet in ETEC-infected piglets: Optimization of growth, immunity, and microbial balance
    Article Snippet: Total bacteria were identified in a final volume of 25 μL with 1 μL forward primer, 1 μL reverse primer, 12.5 μL TB Green Premix EX Taq II Fast (TaKaRa Biotechnology Co., Ltd., Dalian, Liaoning, China), 1 μL template DNA, and 9.5 μL nuclease-free water. .. The thermal cycling parameters were as follows: pre-denaturation step at 95 ◦ C for 30 s and 40 cycles of 95 ◦ C for 5 s, 60 ◦ C for 30 s. For the quantification of Lactobacillus, E. coli, Bacillus, and Bifidobacterium, real-time quantificative PCR was conducted in a 20 μL volume with 0.4 μL probe enhancer solution, 0.2 μL ROX Reference Dcye, 0.6 μL forward and 0.6 μL reverse primer, 10 μL SuperReal Premix (Tiangen Biotech Co., Ltd., Beijing, China), 1 μL template DNA, and 7.2 μL nuclease-free water. ..

    Article Title: Integrative analysis of CUT&Tag-seq and RNA-seq reveals widespread H3K27ac upregulation associated with the activation of muscle-growth-related transcriptional programs in broiler breast muscle.
    Article Snippet: Quantitative real-time PCR was performed using a SYBR Green detection system on a CFX96 Real-Time PCR platform (Bio-Rad). .. Each 20 μL PCR reaction consisted of 10 μL of 2 × Talent qPCR PreMix (SYBR Green, TIANGEN), 0.6 μL of forward primer and 0.6 μL of reverse primer (10 μM each), 1 μL of diluted cDNA template (approximately 400 ng), and RNase-free water to volume. ..

    Article Title: Integrative analysis of CUT&Tag-seq and RNA-seq reveals widespread H3K27ac upregulation associated with the activation of muscle-growth-related transcriptional programs in broiler breast muscle
    Article Snippet: Quantitative real-time PCR was performed using a SYBR Green detection system on a CFX96 Real-Time PCR platform (Bio-Rad). .. Each 20 μL PCR reaction consisted of 10 μL of 2 × Talent qPCR PreMix (SYBR Green, TIANGEN), 0.6 μL of forward primer and 0.6 μL of reverse primer (10 μM each), 1 μL of diluted cDNA template (approximately 400 ng), and RNase-free water to volume. ..

    other:

    Article Title: A long noncoding RNA modulates anthocyanin biosynthesis in Camellia sinensis.
    Article Snippet: The RT-qRT-PCR experiments were performed with a reaction AR TI CL E IN P RE SS system (20 μl) included 0.5 μl (250 nM) of each forward or reverse primer, 2 μl of cDNA template, 10 μl of 2× Tag PCR MasterMix Il (TIANGEN), and ddH2O, using a qTower 2.2 (Analytik Jena AG, Jena, Germany).

    Real-time Polymerase Chain Reaction:

    Article Title: Integrative analysis of CUT&Tag-seq and RNA-seq reveals widespread H3K27ac upregulation associated with the activation of muscle-growth-related transcriptional programs in broiler breast muscle.
    Article Snippet: Quantitative real-time PCR was performed using a SYBR Green detection system on a CFX96 Real-Time PCR platform (Bio-Rad). .. Each 20 μL PCR reaction consisted of 10 μL of 2 × Talent qPCR PreMix (SYBR Green, TIANGEN), 0.6 μL of forward primer and 0.6 μL of reverse primer (10 μM each), 1 μL of diluted cDNA template (approximately 400 ng), and RNase-free water to volume. ..

    Article Title: Integrative analysis of CUT&Tag-seq and RNA-seq reveals widespread H3K27ac upregulation associated with the activation of muscle-growth-related transcriptional programs in broiler breast muscle
    Article Snippet: Quantitative real-time PCR was performed using a SYBR Green detection system on a CFX96 Real-Time PCR platform (Bio-Rad). .. Each 20 μL PCR reaction consisted of 10 μL of 2 × Talent qPCR PreMix (SYBR Green, TIANGEN), 0.6 μL of forward primer and 0.6 μL of reverse primer (10 μM each), 1 μL of diluted cDNA template (approximately 400 ng), and RNase-free water to volume. ..

    SYBR Green Assay:

    Article Title: Integrative analysis of CUT&Tag-seq and RNA-seq reveals widespread H3K27ac upregulation associated with the activation of muscle-growth-related transcriptional programs in broiler breast muscle.
    Article Snippet: Quantitative real-time PCR was performed using a SYBR Green detection system on a CFX96 Real-Time PCR platform (Bio-Rad). .. Each 20 μL PCR reaction consisted of 10 μL of 2 × Talent qPCR PreMix (SYBR Green, TIANGEN), 0.6 μL of forward primer and 0.6 μL of reverse primer (10 μM each), 1 μL of diluted cDNA template (approximately 400 ng), and RNase-free water to volume. ..

    Article Title: Integrative analysis of CUT&Tag-seq and RNA-seq reveals widespread H3K27ac upregulation associated with the activation of muscle-growth-related transcriptional programs in broiler breast muscle
    Article Snippet: Quantitative real-time PCR was performed using a SYBR Green detection system on a CFX96 Real-Time PCR platform (Bio-Rad). .. Each 20 μL PCR reaction consisted of 10 μL of 2 × Talent qPCR PreMix (SYBR Green, TIANGEN), 0.6 μL of forward primer and 0.6 μL of reverse primer (10 μM each), 1 μL of diluted cDNA template (approximately 400 ng), and RNase-free water to volume. ..



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