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STEMCELL Technologies Inc specific negative separation kit rosettesep
Specific Negative Separation Kit Rosettesep, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rosettesep+separation+kit/rosettesep+separation+system/pmc09772689-91-9-13
Average 90 stars, based on 1 article reviews
specific negative separation kit rosettesep - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

Purification:

Article Title: Immunomodulatory drugs activate NK cells via both Zap-70 and cereblon-dependent pathways
Article Snippet: Peripheral blood mononuclear cells (PBMCs) were obtained from healthy volunteers using Ficoll-Paque PLUS density gradient media (GE Healthcare, Uppsala, Sweden). .. T-cells and NK cells were further purified from PBMCs by negative selection procedure using RosetteSep Separation System (StemCell Technologies, Vancouver, Canada). ..

Article Title: Targeting of colorectal cancer organoids with zoledronic acid conjugated to the anti-EGFR antibody cetuximab
Article Snippet: .. T lymphocytes were purified from PBMC using the specific negative separation kit (RosetteSep, STEMCELL Technologies, Vancouver, Canada) leading to >98% pure CD2 + CD3 + T-cell populations and incubated overnight in Roswell Park Memorial Institute (RPMI) 1640 (supplemented with 10% fetal bovine serum, FBS, penicillin/streptomycin and L-glutamin, all from Gibco) to allow adherence of the residual undetectable monocyte component and avoid their presence in stimulation experiments. .. T lymphocytes were purified from PBMC using the specific negative separation kit (RosetteSep, STEMCELL Technologies, Vancouver, Canada) leading to >98% pure CD2 + CD3 + T-cell populations and incubated overnight in Roswell Park Memorial Institute (RPMI) 1640 (supplemented with 10% fetal bovine serum, FBS, penicillin/streptomycin and L-glutamin, all from Gibco) to allow adherence of the residual undetectable monocyte component and avoid their presence in stimulation experiments.

Selection:

Article Title: Immunomodulatory drugs activate NK cells via both Zap-70 and cereblon-dependent pathways
Article Snippet: Peripheral blood mononuclear cells (PBMCs) were obtained from healthy volunteers using Ficoll-Paque PLUS density gradient media (GE Healthcare, Uppsala, Sweden). .. T-cells and NK cells were further purified from PBMCs by negative selection procedure using RosetteSep Separation System (StemCell Technologies, Vancouver, Canada). ..

Article Title: HIV-1 latency is established preferentially in minimally activated and non-dividing cells during productive infection of primary CD4 T cells
Article Snippet: .. Total CD4 T lymphocytes were isolated by negative selection from whole blood buffy coats using the RosetteSep cell separation procedure (StemCell Technologies), followed by density gradient centrifugation with Histopaque 1077 (Sigma-Aldrich) or Lymphoprep Cell Separation Medium (Accurate Chemical). ..

Article Title: HIV-1 latency is established preferentially in minimally activated and non-dividing cells during productive infection of primary CD4 T cells.
Article Snippet: .. Total CD4 T lymphocytes were isolated by negative selection from whole blood buffy coats using the RosetteSep cell separation procedure (StemCell Technologies), followed by density gradient centrifugation with Histopaque 1077 (SigmaAldrich) or Lymphoprep Cell Separation Medium (Accurate Chemical). ..

Isolation:

Article Title: HIV-1 latency is established preferentially in minimally activated and non-dividing cells during productive infection of primary CD4 T cells
Article Snippet: .. Total CD4 T lymphocytes were isolated by negative selection from whole blood buffy coats using the RosetteSep cell separation procedure (StemCell Technologies), followed by density gradient centrifugation with Histopaque 1077 (Sigma-Aldrich) or Lymphoprep Cell Separation Medium (Accurate Chemical). ..

Article Title: HIV-1 latency is established preferentially in minimally activated and non-dividing cells during productive infection of primary CD4 T cells.
Article Snippet: .. Total CD4 T lymphocytes were isolated by negative selection from whole blood buffy coats using the RosetteSep cell separation procedure (StemCell Technologies), followed by density gradient centrifugation with Histopaque 1077 (SigmaAldrich) or Lymphoprep Cell Separation Medium (Accurate Chemical). ..

Gradient Centrifugation:

Article Title: HIV-1 latency is established preferentially in minimally activated and non-dividing cells during productive infection of primary CD4 T cells
Article Snippet: .. Total CD4 T lymphocytes were isolated by negative selection from whole blood buffy coats using the RosetteSep cell separation procedure (StemCell Technologies), followed by density gradient centrifugation with Histopaque 1077 (Sigma-Aldrich) or Lymphoprep Cell Separation Medium (Accurate Chemical). ..

Article Title: HIV-1 latency is established preferentially in minimally activated and non-dividing cells during productive infection of primary CD4 T cells.
Article Snippet: .. Total CD4 T lymphocytes were isolated by negative selection from whole blood buffy coats using the RosetteSep cell separation procedure (StemCell Technologies), followed by density gradient centrifugation with Histopaque 1077 (SigmaAldrich) or Lymphoprep Cell Separation Medium (Accurate Chemical). ..

other:

Article Title: p53-related protein kinase confers poor prognosis and represents a novel therapeutic target in multiple myeloma
Article Snippet: Patient MM cells were purified from BM aspirates by negative selection (RosetteSep Separation System, StemCell Technologies, Vancouver, Canada).

Article Title: A 17q12 Allele Is Associated with Altered NK Cell Subsets and Function
Article Snippet: The capacity of NK cells to secrete cytokines and degranulate was examined following stimulation of PBMCs with different NK target cell lines, each at a 10:1 E:T ratio: 1) Ab-dependent cell-mediated cytotoxicity (p815 cells; a mouse leukemic cell line [American Type Culture Collection], preincubated for 1 h with 1 mg/ml p815-specific Ab [Abcam, Cambridge, MA]), 2) MHC-devoid target cell line (K562 cells; American Type Culture Collection), and 3) MHC class I-deficient B cell line (721.221 cells; American Type Culture Collection).

Article Title: Compositions and methods for treating multiple myeloma
Article Snippet: Patient MM cells were purified from bone marrow (BM) aspirates by negative selection (RosetteSep Separation System®, StemCell Technologies, Vancouver, Canada).

Incubation:

Article Title: Targeting of colorectal cancer organoids with zoledronic acid conjugated to the anti-EGFR antibody cetuximab
Article Snippet: .. T lymphocytes were purified from PBMC using the specific negative separation kit (RosetteSep, STEMCELL Technologies, Vancouver, Canada) leading to >98% pure CD2 + CD3 + T-cell populations and incubated overnight in Roswell Park Memorial Institute (RPMI) 1640 (supplemented with 10% fetal bovine serum, FBS, penicillin/streptomycin and L-glutamin, all from Gibco) to allow adherence of the residual undetectable monocyte component and avoid their presence in stimulation experiments. .. T lymphocytes were purified from PBMC using the specific negative separation kit (RosetteSep, STEMCELL Technologies, Vancouver, Canada) leading to >98% pure CD2 + CD3 + T-cell populations and incubated overnight in Roswell Park Memorial Institute (RPMI) 1640 (supplemented with 10% fetal bovine serum, FBS, penicillin/streptomycin and L-glutamin, all from Gibco) to allow adherence of the residual undetectable monocyte component and avoid their presence in stimulation experiments.



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