rosettesep negative isolation kit (STEMCELL Technologies Inc)

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STEMCELL Technologies Inc rosettesep negative isolation kit
Rosettesep Negative Isolation Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rosettesep+negative+isolation+kit/rosettesep+human+b+cell+enrichment+cocktail/pmc12169587-258-2-6
Average 90 stars, based on 1 article reviews

rosettesep negative isolation kit - by Bioz Stars, 2026-10

90/100 stars

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Isolation:

Article Title: Tuning antibody stability and function by rational designs of framework mutations
Article Snippet: Optical absorbance was read on FLUOstar Omega spectrophotometer (BMG Labtech). .. Natural killer (NK) cells were isolated using the RosetteSep TM Human NK Cell Enrichment Cocktail (STEMCELL Technologies) following the manufacturer’s instructions. ..

Article Title: Tuning antibody stability and function by rational designs of framework mutations.
Article Snippet: Optical absorbance was read on FLUOstar Omega spectrophotometer (BMG Labtech). .. Natural killer (NK) cells were isolated using the RosetteSepTM Human NK Cell Enrichment Cocktail (STEMCELL Technologies) following the manufacturer’s instructions. ..

Article Title: Whole-genome CRISPR screening identifies genetic manipulations to reduce immune rejection of stem cell-derived islets
Article Snippet: PBMCs or isolated cells were cultured in T-cell media: X-VIVO 10 (Lonza, 04-380Q) media supplemented with 5% Human AB Serum (Valley Biomedical, HP1022HI), 5% Fetal Bovine Serum (ThermoFisher Scientific, A3840101), 1% Penicillin/Streptomycin (ThermoFisher Scientific, 15070063), GlutaMAX (ThermoFisher Scientific, 35050061), MEM NonEssential Amino Acids (ThermoFisher Scientific, 11140050). .. Human PBMCs were isolated using the density gradient medium, Ficoll-Paque Plus (GE health care life sciences, 17144002) and the SepMate tubes (Stem Cell Technologies, 85450); T cells and NK cells were isolated using RosetteSep Human T Cell Isolation Kit (Stem Cell Technologies, 15061 and 15065, respectively). .. SC-islet clusters were dissociated using TrypLE Express (Gibco, 12604013) at 37 °C, mechanically disrupted to form single cells, ten thousand cells were plated per well on 96-well V-shaped bottom plates and allowed to reaggregate for 48hrs in S3 media.

Article Title: Development of a 3D in vitro wound healing model to assess the effect of ADSC-EVs on vascularisation
Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were isolated from the buffy coat of 20 mL of whole blood using RosetteSep human enrichment cocktail (STEMCELL Technologies, BC, Canada) and density gradient centrifugation (Ficoll-Paque PLUS, Bio-Strategy, Australia) as per the manufacturer’s instructions. ..

Article Title: Chimeric PD-1 receptor redirects primary T cells against childhood solid tumors but not to PD-1 ligand-positive CD80-coexpressing cells
Article Snippet: .. Peripheral blood mononuclear cells were collected from healthy volunteers after obtaining written informed consent, and T cells were isolated using RosetteSepTM Human T Cell Enrichment Cocktail (STEMCELL Technology, Vancouver, BC, Canada). .. T cells were incubated for 48–72 h with Dynabeads human T-activator CD3/CD28 (Thermo Fisher Scientific, Waltham, MA, USA) in the presence of 200 IU/ml recombinant human interleukin-2 (rhIL-2; Peprotech, Cranbury, NJ, USA) in RPMI-1640 with 10% FBS.

Article Title: Anti-IL-27 antibodies and uses thereof
Article Snippet: .. Resting T-cells were isolated from human buffy coats using RosetteSepTM Human T cell Enrichment Cocktail (Stemcell #15061). .. At the conclusion of the assay, the cell pellets were stained with 50 μL per well of staining cocktail as described in the Table 10 below: TABLE 10 Biolegend Catalog # Antibody Target Color Dilution 345006 TIM3 PE 1:100 555349 CD4 APC 1:100 329714 PD-L1 BV421 1:100 555366 CD8 FITC 1:100 The plate was agitated on a plate shaker for 30 seconds at 600 RPM and the plate was incubated for 30 minutes at 4° C. in the dark.

Cell Isolation:

Gradient Centrifugation:

other:

Article Title: Genome sequencing reveals CCDC88A variants in malformations of cortical development and immune dysfunction
Article Snippet: CD4+ cells were isolated from whole blood with RosetteSepTM Human CD4+ T Cell Enrichment Cocktail (Stemcell Technologies, Vancouver, bc , Canada) according to the manufacturer.

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