Cell Culture:Article Title: Biomimetic Double-Protein Semi-IPN Hydrogels: Synergistic Protein-Polysaccharide Biomatrices for Healing, Immunomodulation, and Mineralization.
Article Snippet: This study introduces a biomimetic dualprotein semiinterpenetrating polymer network (semiIPN) platform integrating collagen (C) and the phosphorylated globular protein ovalbumin within a bioactive polyurethane (PU) crosslinked matrix.. Unlike previously reported collagen–PU–polysaccharide systems, the structural incorporation of a second protein phase enables cooperative regulation of mineral nucleation, interfacial charge distribution, and biological response.. Three polysaccharides—starch (CA–A), carboxymethyl cellulose (CA–CMC), and xanthan gum (CA–GX)—were incorporated to modulate network architecture and functionality.
Modification:Article Title: Biomimetic Double-Protein Semi-IPN Hydrogels: Synergistic Protein-Polysaccharide Biomatrices for Healing, Immunomodulation, and Mineralization.
Article Snippet: This study introduces a biomimetic dualprotein semiinterpenetrating polymer network (semiIPN) platform integrating collagen (C) and the phosphorylated globular protein ovalbumin within a bioactive polyurethane (PU) crosslinked matrix.. Unlike previously reported collagen–PU–polysaccharide systems, the structural incorporation of a second protein phase enables cooperative regulation of mineral nucleation, interfacial charge distribution, and biological response.. Three polysaccharides—starch (CA–A), carboxymethyl cellulose (CA–CMC), and xanthan gum (CA–GX)—were incorporated to modulate network architecture and functionality.
Expressing:
Construct:
Article Title: Structural Basis of Condensin Recruitment for X Chromosome Repression
Article Snippet: .. The SDC-3 C construct with an amino-terminal His 8 -mNeonGreen-3C tag was expressed in E. coli Rosetta (DE3) pLysS (Merck) grown at 37 °C in 2×TY medium supplemented with ZnCl 2 at a final concentration of 25 μM. ..
Transformation Assay:
Concentration Assay:Article Title: Structural Basis of Condensin Recruitment for X Chromosome Repression
Article Snippet: .. The SDC-3 C construct with an amino-terminal His 8 -mNeonGreen-3C tag was expressed in E. coli Rosetta (DE3) pLysS (Merck) grown at 37 °C in 2×TY medium supplemented with ZnCl 2 at a final concentration of 25 μM. ..
Virus:Article Title: Protocol for synthesizing and purifying short-length poly(ADP)ribose polymer using fast protein liquid chromatography.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains E. coli Rosetta2 (DE3) Merck Cat#71397-3CN Chemicals, peptides, and recombinant proteins Luria Bertani (LB) Broth HiMedia Cat#M1245-500G Isopropyl β-D-1-thiogalactopyranoside (IPTG) SRL Cat#54110 Chloramphenicol VWR Life Sciences Cat#0230-100G Kanamycin SRL Cat#99311 Nickel (II) Sulphate Hexahydrate HiMedia Cat#10101970 Imidazole HiMedia Cat#288324 Acrylamide HiMedia Cat#79061 β-Mercaptoethanol HiMedia Cat#60242 Glycerol HiMedia Cat#56815 Bis-acrylamide HiMedia Cat#110269 Tris Hydrochloride (Tris HCl) HiMedia Cat#1185531 Tris Base HiMedia Cat#77861 Ammonium Per Sulphate (APS) SRL Cat#7727540 Nicotinamide Adenine Dinucleotide (NAD + ) HiMedia Cat#53849 Trichloroacetic acid (TCA) HiMedia Cat#76039 Sodium Dodecyl Sulphate (SDS) SRL Cat#151213 Potassium Hydroxide (KOH) Sigma Cat#1310583 Ethylenediaminetetraacetic acid (EDTA) HiMedia Cat#6381926 Phenol SRL Cat#108952 Chloroform SRL Cat#67663 Isoamyl alcohol HiMedia Cat#123513 Ethanol Hayman Cat#F204325 Phenylmethylsulfonyl fluoride (PMSF) HiMedia Cat#329986 EDTA-free Protease Inhibitor Cocktail Roche Cat#11836170001 Lysozyme SRL Cat#12650883 Proteinase K MagGenome Cat#MG19PK-1 Silver Nitrate SRL Cat#7761888 Sodium Carbonate Anhydrous HiMedia Cat#497198 Magnesium Chloride Hexahydrate (MgCl2) HiMedia Cat#7791186 Acetic Acid HiMedia Cat$64197 Formaldehyde Sigma Cat#605001005 Nitric acid Sigma Cat#1004410250 Unstained Protein Marker Thermo Scientific Cat#26610 Oligonucleotides (5 ′ -3 ′ ) 16-mer DSB DNA (5 ′ –3 ′ ) Strand 1: GACGACCCGGAGCACA Strand 2: TGTGCTCCGGGTCGTC Huwel Lifesciences Custom synthesis 10mer-ssDNA: 5 ′ -CTAGAAGATG-3 ′ (Continued on next page) 2 STAR Protocols 7, 104319, March 20, 2026 ..
Recombinant:Article Title: Protocol for synthesizing and purifying short-length poly(ADP)ribose polymer using fast protein liquid chromatography.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains E. coli Rosetta2 (DE3) Merck Cat#71397-3CN Chemicals, peptides, and recombinant proteins Luria Bertani (LB) Broth HiMedia Cat#M1245-500G Isopropyl β-D-1-thiogalactopyranoside (IPTG) SRL Cat#54110 Chloramphenicol VWR Life Sciences Cat#0230-100G Kanamycin SRL Cat#99311 Nickel (II) Sulphate Hexahydrate HiMedia Cat#10101970 Imidazole HiMedia Cat#288324 Acrylamide HiMedia Cat#79061 β-Mercaptoethanol HiMedia Cat#60242 Glycerol HiMedia Cat#56815 Bis-acrylamide HiMedia Cat#110269 Tris Hydrochloride (Tris HCl) HiMedia Cat#1185531 Tris Base HiMedia Cat#77861 Ammonium Per Sulphate (APS) SRL Cat#7727540 Nicotinamide Adenine Dinucleotide (NAD + ) HiMedia Cat#53849 Trichloroacetic acid (TCA) HiMedia Cat#76039 Sodium Dodecyl Sulphate (SDS) SRL Cat#151213 Potassium Hydroxide (KOH) Sigma Cat#1310583 Ethylenediaminetetraacetic acid (EDTA) HiMedia Cat#6381926 Phenol SRL Cat#108952 Chloroform SRL Cat#67663 Isoamyl alcohol HiMedia Cat#123513 Ethanol Hayman Cat#F204325 Phenylmethylsulfonyl fluoride (PMSF) HiMedia Cat#329986 EDTA-free Protease Inhibitor Cocktail Roche Cat#11836170001 Lysozyme SRL Cat#12650883 Proteinase K MagGenome Cat#MG19PK-1 Silver Nitrate SRL Cat#7761888 Sodium Carbonate Anhydrous HiMedia Cat#497198 Magnesium Chloride Hexahydrate (MgCl2) HiMedia Cat#7791186 Acetic Acid HiMedia Cat$64197 Formaldehyde Sigma Cat#605001005 Nitric acid Sigma Cat#1004410250 Unstained Protein Marker Thermo Scientific Cat#26610 Oligonucleotides (5 ′ -3 ′ ) 16-mer DSB DNA (5 ′ –3 ′ ) Strand 1: GACGACCCGGAGCACA Strand 2: TGTGCTCCGGGTCGTC Huwel Lifesciences Custom synthesis 10mer-ssDNA: 5 ′ -CTAGAAGATG-3 ′ (Continued on next page) 2 STAR Protocols 7, 104319, March 20, 2026 ..
Protease Inhibitor:Article Title: Protocol for synthesizing and purifying short-length poly(ADP)ribose polymer using fast protein liquid chromatography.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains E. coli Rosetta2 (DE3) Merck Cat#71397-3CN Chemicals, peptides, and recombinant proteins Luria Bertani (LB) Broth HiMedia Cat#M1245-500G Isopropyl β-D-1-thiogalactopyranoside (IPTG) SRL Cat#54110 Chloramphenicol VWR Life Sciences Cat#0230-100G Kanamycin SRL Cat#99311 Nickel (II) Sulphate Hexahydrate HiMedia Cat#10101970 Imidazole HiMedia Cat#288324 Acrylamide HiMedia Cat#79061 β-Mercaptoethanol HiMedia Cat#60242 Glycerol HiMedia Cat#56815 Bis-acrylamide HiMedia Cat#110269 Tris Hydrochloride (Tris HCl) HiMedia Cat#1185531 Tris Base HiMedia Cat#77861 Ammonium Per Sulphate (APS) SRL Cat#7727540 Nicotinamide Adenine Dinucleotide (NAD + ) HiMedia Cat#53849 Trichloroacetic acid (TCA) HiMedia Cat#76039 Sodium Dodecyl Sulphate (SDS) SRL Cat#151213 Potassium Hydroxide (KOH) Sigma Cat#1310583 Ethylenediaminetetraacetic acid (EDTA) HiMedia Cat#6381926 Phenol SRL Cat#108952 Chloroform SRL Cat#67663 Isoamyl alcohol HiMedia Cat#123513 Ethanol Hayman Cat#F204325 Phenylmethylsulfonyl fluoride (PMSF) HiMedia Cat#329986 EDTA-free Protease Inhibitor Cocktail Roche Cat#11836170001 Lysozyme SRL Cat#12650883 Proteinase K MagGenome Cat#MG19PK-1 Silver Nitrate SRL Cat#7761888 Sodium Carbonate Anhydrous HiMedia Cat#497198 Magnesium Chloride Hexahydrate (MgCl2) HiMedia Cat#7791186 Acetic Acid HiMedia Cat$64197 Formaldehyde Sigma Cat#605001005 Nitric acid Sigma Cat#1004410250 Unstained Protein Marker Thermo Scientific Cat#26610 Oligonucleotides (5 ′ -3 ′ ) 16-mer DSB DNA (5 ′ –3 ′ ) Strand 1: GACGACCCGGAGCACA Strand 2: TGTGCTCCGGGTCGTC Huwel Lifesciences Custom synthesis 10mer-ssDNA: 5 ′ -CTAGAAGATG-3 ′ (Continued on next page) 2 STAR Protocols 7, 104319, March 20, 2026 ..
Marker:Article Title: Protocol for synthesizing and purifying short-length poly(ADP)ribose polymer using fast protein liquid chromatography.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains E. coli Rosetta2 (DE3) Merck Cat#71397-3CN Chemicals, peptides, and recombinant proteins Luria Bertani (LB) Broth HiMedia Cat#M1245-500G Isopropyl β-D-1-thiogalactopyranoside (IPTG) SRL Cat#54110 Chloramphenicol VWR Life Sciences Cat#0230-100G Kanamycin SRL Cat#99311 Nickel (II) Sulphate Hexahydrate HiMedia Cat#10101970 Imidazole HiMedia Cat#288324 Acrylamide HiMedia Cat#79061 β-Mercaptoethanol HiMedia Cat#60242 Glycerol HiMedia Cat#56815 Bis-acrylamide HiMedia Cat#110269 Tris Hydrochloride (Tris HCl) HiMedia Cat#1185531 Tris Base HiMedia Cat#77861 Ammonium Per Sulphate (APS) SRL Cat#7727540 Nicotinamide Adenine Dinucleotide (NAD + ) HiMedia Cat#53849 Trichloroacetic acid (TCA) HiMedia Cat#76039 Sodium Dodecyl Sulphate (SDS) SRL Cat#151213 Potassium Hydroxide (KOH) Sigma Cat#1310583 Ethylenediaminetetraacetic acid (EDTA) HiMedia Cat#6381926 Phenol SRL Cat#108952 Chloroform SRL Cat#67663 Isoamyl alcohol HiMedia Cat#123513 Ethanol Hayman Cat#F204325 Phenylmethylsulfonyl fluoride (PMSF) HiMedia Cat#329986 EDTA-free Protease Inhibitor Cocktail Roche Cat#11836170001 Lysozyme SRL Cat#12650883 Proteinase K MagGenome Cat#MG19PK-1 Silver Nitrate SRL Cat#7761888 Sodium Carbonate Anhydrous HiMedia Cat#497198 Magnesium Chloride Hexahydrate (MgCl2) HiMedia Cat#7791186 Acetic Acid HiMedia Cat$64197 Formaldehyde Sigma Cat#605001005 Nitric acid Sigma Cat#1004410250 Unstained Protein Marker Thermo Scientific Cat#26610 Oligonucleotides (5 ′ -3 ′ ) 16-mer DSB DNA (5 ′ –3 ′ ) Strand 1: GACGACCCGGAGCACA Strand 2: TGTGCTCCGGGTCGTC Huwel Lifesciences Custom synthesis 10mer-ssDNA: 5 ′ -CTAGAAGATG-3 ′ (Continued on next page) 2 STAR Protocols 7, 104319, March 20, 2026 ..
other:
|
