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rnaeasy plus universal mini kit  (Qiagen)


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  • 99

    Structured Review

    Qiagen rnaeasy plus universal mini kit
    KEY RESOURCES TABLE
    Rnaeasy Plus Universal Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 17961 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rneasy+microarray+tissue+mini+kit/RNeasy+Plus+Universal+Mini+Kit/pmc07312756-23-0-6
    Average 99 stars, based on 17961 article reviews
    rnaeasy plus universal mini kit - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "Inhibition of Upf2-Dependent Nonsense-Mediated Decay Leads to Behavioral and Neurophysiological Abnormalities by Activating the Immune Response"

    Article Title: Inhibition of Upf2-Dependent Nonsense-Mediated Decay Leads to Behavioral and Neurophysiological Abnormalities by Activating the Immune Response

    Journal: Neuron

    doi: 10.1016/j.neuron.2019.08.027

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Recombinant, Sequencing, SYBR Green Assay, Multiplex Assay, Microarray, Knock-Out, Software

    Related Articles

    Isolation:

    Article Title: Female metabolic resilience and male-biased protein quality defects under hypoxia in human brain organoids
    Article Snippet: .. Total RNA was isolated from COs using the RNeasy Plus Universal Tissue Mini Kit (Qiagen) in combination with the Precellys Evolution tissue homogenizer (Bertin). .. The RNA concentration and purity were assessed with a NanoDrop ND-1000 spectrophotometer by measuring absorbance at 260 and 280 nm (NanoDrop Technologies), with A260/280 ratios falling between 1.8 and 2.2.

    Article Title: Detection of nuclear STING in cultured human cells and in the normal and cancer tissues
    Article Snippet: .. RNAs were isolated using RNeasy Plus RNA isolation kit (Qiagen). .. 2 μg of RNA was reverse-transcribed using High Capacity cDNA Reverse Transcription kit (Applied Biosystems) per manufacturer’s protocol. cDNAs were diluted 1:10 and 1 μL of diluted cDNA was used per qPCR reaction.

    Article Title: Dual-function oral nanotherapeutic mitigates sepsis-like multi-organ failure by targeting inflammatory and fibrotic pathways
    Article Snippet: The supernatant was harvested and assayed for alkaline phosphatase and amylase using an automated chemistry analyzer (Vet Axcel, Alfa Wassermann). .. Real-time polymerase chain reaction (PCR): 30 mg of each tissue was homogenized in Qiazol reagent (catalog number: 79306, Qiagen) followed by RNA isolation using RNeasy plus universal mini kit (catalog number: 73404, Qiagen). ..

    RNA Extraction:

    Article Title: PRKN/parkin-mediated control of SNCA (synuclein alpha) and chaperone-mediated autophagy are defective in cellular, mice models and Parkinson disease-affected brains.
    Article Snippet: Forty-eight h later, the RNAs were extracted using RNeasy Mini Kit (Qiagen, 74004) according to manufacturer instructions. .. RNAlater (Qiagen, AM7024) tissue or powder aliquots from stabilized mice brain (one hemisphere, striatum) were homogenized using MagNA Lyser Green beads tubes (Roche, 03358941001) via MagNA Lyser instrument (Roche, 03358976001) for 45 s at 4487 g in RNA extraction lysis buffer, then RNA extraction was performed using RNeasy Plus Universal Mini kit (Qiagen, 73404) manufacturer’s instructions. .. A total amount of 2 μg of RNA was reversetranscribed in cDNA using Oligo-dT primer (Promega, C11001) and reverse transcriptase (Promega, A5002) following manufacturer’s guidelines.

    Lysis:

    Article Title: PRKN/parkin-mediated control of SNCA (synuclein alpha) and chaperone-mediated autophagy are defective in cellular, mice models and Parkinson disease-affected brains.
    Article Snippet: Forty-eight h later, the RNAs were extracted using RNeasy Mini Kit (Qiagen, 74004) according to manufacturer instructions. .. RNAlater (Qiagen, AM7024) tissue or powder aliquots from stabilized mice brain (one hemisphere, striatum) were homogenized using MagNA Lyser Green beads tubes (Roche, 03358941001) via MagNA Lyser instrument (Roche, 03358976001) for 45 s at 4487 g in RNA extraction lysis buffer, then RNA extraction was performed using RNeasy Plus Universal Mini kit (Qiagen, 73404) manufacturer’s instructions. .. A total amount of 2 μg of RNA was reversetranscribed in cDNA using Oligo-dT primer (Promega, C11001) and reverse transcriptase (Promega, A5002) following manufacturer’s guidelines.

    Real-time Polymerase Chain Reaction:

    Article Title: Dual-function oral nanotherapeutic mitigates sepsis-like multi-organ failure by targeting inflammatory and fibrotic pathways
    Article Snippet: The supernatant was harvested and assayed for alkaline phosphatase and amylase using an automated chemistry analyzer (Vet Axcel, Alfa Wassermann). .. Real-time polymerase chain reaction (PCR): 30 mg of each tissue was homogenized in Qiazol reagent (catalog number: 79306, Qiagen) followed by RNA isolation using RNeasy plus universal mini kit (catalog number: 73404, Qiagen). ..

    Polymerase Chain Reaction:

    Article Title: Dual-function oral nanotherapeutic mitigates sepsis-like multi-organ failure by targeting inflammatory and fibrotic pathways
    Article Snippet: The supernatant was harvested and assayed for alkaline phosphatase and amylase using an automated chemistry analyzer (Vet Axcel, Alfa Wassermann). .. Real-time polymerase chain reaction (PCR): 30 mg of each tissue was homogenized in Qiazol reagent (catalog number: 79306, Qiagen) followed by RNA isolation using RNeasy plus universal mini kit (catalog number: 73404, Qiagen). ..



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