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rnaeasy plus universal mini kit  (Qiagen)


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  • 99

    Structured Review

    Qiagen rnaeasy plus universal mini kit
    KEY RESOURCES TABLE
    Rnaeasy Plus Universal Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 18014 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rneasy+microarray+tissue+micro+kit/RNeasy+Plus+Universal+Mini+Kit/pmc07312756-23-0-6
    Average 99 stars, based on 18014 article reviews
    rnaeasy plus universal mini kit - by Bioz Stars, 2026-11
    99/100 stars

    Images

    1) Product Images from "Inhibition of Upf2-Dependent Nonsense-Mediated Decay Leads to Behavioral and Neurophysiological Abnormalities by Activating the Immune Response"

    Article Title: Inhibition of Upf2-Dependent Nonsense-Mediated Decay Leads to Behavioral and Neurophysiological Abnormalities by Activating the Immune Response

    Journal: Neuron

    doi: 10.1016/j.neuron.2019.08.027

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Recombinant, Sequencing, SYBR Green Assay, Multiplex Assay, Microarray, Knock-Out, Software

    Related Articles

    Bulk RNA Sequencing:

    Article Title: ACKR3 Establishes a Squamous-Restricted Immune Checkpoint Axis in Lung Squamous Cell Carcinoma
    Article Snippet: .. 271 272 273 Bulk RNA Sequencing and Data Analysis 274 275 Total RNA was extracted from PL and PLA murine cell lines using the RNeasy Plus 276 Universal Mini Kit (QIAGEN) according to the manufacturer’s instructions. .. cDNA 277 synthesis was performed using the SMARTer PCR cDNA Synthesis Kit (Clontech), 278 followed by library preparation with the Nextera XT DNA Library Preparation Kit (Illumina), 279 following standard protocols.

    Analysis:

    Article Title: ACKR3 Establishes a Squamous-Restricted Immune Checkpoint Axis in Lung Squamous Cell Carcinoma
    Article Snippet: .. 271 272 273 Bulk RNA Sequencing and Data Analysis 274 275 Total RNA was extracted from PL and PLA murine cell lines using the RNeasy Plus 276 Universal Mini Kit (QIAGEN) according to the manufacturer’s instructions. .. cDNA 277 synthesis was performed using the SMARTer PCR cDNA Synthesis Kit (Clontech), 278 followed by library preparation with the Nextera XT DNA Library Preparation Kit (Illumina), 279 following standard protocols.

    Article Title: IL-12-producing iPSC-derived antigen-presenting cells enhance antitumor immunity and enable off-the-shelf immunotherapy.
    Article Snippet: Please cite this article as: Yamamoto M, Zhang R, Kobayashi T, Li Y, Xu H, Mashima H, Saito K, Kariya M, Ide M, Hagiya Y, Fukuda E, Uemura S, Osako Y, Nakaki R, Nakatsura T, Fukushima S, Kaneko S, Hotta A, Ohdan H, Uemura.. Y, IL-12–producing iPSC-derived antigen-presenting cells enhance antitumor immunity and enable off-the-shelf immunotherapy, Molecular Therapy (2026), doi: https:// doi.org/10.1016/j.ymthe.2026.10.017.

    Proximity Ligation Assay:

    Article Title: ACKR3 Establishes a Squamous-Restricted Immune Checkpoint Axis in Lung Squamous Cell Carcinoma
    Article Snippet: .. 271 272 273 Bulk RNA Sequencing and Data Analysis 274 275 Total RNA was extracted from PL and PLA murine cell lines using the RNeasy Plus 276 Universal Mini Kit (QIAGEN) according to the manufacturer’s instructions. .. cDNA 277 synthesis was performed using the SMARTer PCR cDNA Synthesis Kit (Clontech), 278 followed by library preparation with the Nextera XT DNA Library Preparation Kit (Illumina), 279 following standard protocols.

    Isolation:

    Article Title: Microglia-mediated extracellular matrix disruption drives neuronal dysfunction, motor impairments, and sedation following alcohol abuse.
    Article Snippet: .. Isolation of liver RNA and qRT-PCR Total RNA was isolated from liver tissue using Rneasy Plus Universal Mini Kit (Qiagen) and reverse transcribed with SuperScript IV VILO Master Mix (Invitrogen), followed by amplification using qRT-PCR. .. Power SYBR Green PCR Master Mix (Applied Biosystems) and primers (listed below) at final concentrations of 1 μM were used.

    Quantitative RT-PCR:

    Article Title: Microglia-mediated extracellular matrix disruption drives neuronal dysfunction, motor impairments, and sedation following alcohol abuse.
    Article Snippet: .. Isolation of liver RNA and qRT-PCR Total RNA was isolated from liver tissue using Rneasy Plus Universal Mini Kit (Qiagen) and reverse transcribed with SuperScript IV VILO Master Mix (Invitrogen), followed by amplification using qRT-PCR. .. Power SYBR Green PCR Master Mix (Applied Biosystems) and primers (listed below) at final concentrations of 1 μM were used.

    Tissue:

    Article Title: Microglia-mediated extracellular matrix disruption drives neuronal dysfunction, motor impairments, and sedation following alcohol abuse.
    Article Snippet: .. Isolation of liver RNA and qRT-PCR Total RNA was isolated from liver tissue using Rneasy Plus Universal Mini Kit (Qiagen) and reverse transcribed with SuperScript IV VILO Master Mix (Invitrogen), followed by amplification using qRT-PCR. .. Power SYBR Green PCR Master Mix (Applied Biosystems) and primers (listed below) at final concentrations of 1 μM were used.

    Article Title: Development and Characterization of a Rat Model of Post-traumatic Ankle Osteoarthritis.
    Article Snippet: .. To get sufficient tissue for RNA sequencing, DRGs from 2-3 rats were pooled, and total RNA was extracted using an RNeasy Plus Universal Kit (Qiagen, Germantown, MD) according to the manufacturer's instructions. ..

    Reverse Transcription:

    Article Title: Microglia-mediated extracellular matrix disruption drives neuronal dysfunction, motor impairments, and sedation following alcohol abuse.
    Article Snippet: .. Isolation of liver RNA and qRT-PCR Total RNA was isolated from liver tissue using Rneasy Plus Universal Mini Kit (Qiagen) and reverse transcribed with SuperScript IV VILO Master Mix (Invitrogen), followed by amplification using qRT-PCR. .. Power SYBR Green PCR Master Mix (Applied Biosystems) and primers (listed below) at final concentrations of 1 μM were used.

    Amplification:

    Article Title: Microglia-mediated extracellular matrix disruption drives neuronal dysfunction, motor impairments, and sedation following alcohol abuse.
    Article Snippet: .. Isolation of liver RNA and qRT-PCR Total RNA was isolated from liver tissue using Rneasy Plus Universal Mini Kit (Qiagen) and reverse transcribed with SuperScript IV VILO Master Mix (Invitrogen), followed by amplification using qRT-PCR. .. Power SYBR Green PCR Master Mix (Applied Biosystems) and primers (listed below) at final concentrations of 1 μM were used.

    Cell Culture:

    Article Title: A natural compound derivative targeting HADHA disrupts mitochondrial homeostasis and suppresses renal cell carcinoma.
    Article Snippet: Ara Jo, Sanjeevram Dhandapani, Ziqin Wang, Hyeryeon Jung, Hongjun Jang, Morgan A. Bailey, Andrew Tran, Brandon Tsai, Jinjae Park, Hyejin Lee, Hye-Jin Park, Yoojin Lim, Dolce Feenaghty, Victor M. Moncada, Damu Wu, Keaton Chien, Nancy Chen, Yunsol Jo, Jiazheng Miao, Zhujun Yao, Jihoon Lee, Karthigayan Shanmugasundaram, Gang Greg Wang, Andrew Armstrong, Daniel George, David Howell, Jiaoti Huang, Michael C. Fitzgerald, Jiyong Hong, Jung Wook Park

    Crocin Bleaching Assay:

    Article Title: A natural compound derivative targeting HADHA disrupts mitochondrial homeostasis and suppresses renal cell carcinoma.
    Article Snippet: Ara Jo, Sanjeevram Dhandapani, Ziqin Wang, Hyeryeon Jung, Hongjun Jang, Morgan A. Bailey, Andrew Tran, Brandon Tsai, Jinjae Park, Hyejin Lee, Hye-Jin Park, Yoojin Lim, Dolce Feenaghty, Victor M. Moncada, Damu Wu, Keaton Chien, Nancy Chen, Yunsol Jo, Jiazheng Miao, Zhujun Yao, Jihoon Lee, Karthigayan Shanmugasundaram, Gang Greg Wang, Andrew Armstrong, Daniel George, David Howell, Jiaoti Huang, Michael C. Fitzgerald, Jiyong Hong, Jung Wook Park

    Lysis:

    Article Title: A natural compound derivative targeting HADHA disrupts mitochondrial homeostasis and suppresses renal cell carcinoma.
    Article Snippet: Ara Jo, Sanjeevram Dhandapani, Ziqin Wang, Hyeryeon Jung, Hongjun Jang, Morgan A. Bailey, Andrew Tran, Brandon Tsai, Jinjae Park, Hyejin Lee, Hye-Jin Park, Yoojin Lim, Dolce Feenaghty, Victor M. Moncada, Damu Wu, Keaton Chien, Nancy Chen, Yunsol Jo, Jiazheng Miao, Zhujun Yao, Jihoon Lee, Karthigayan Shanmugasundaram, Gang Greg Wang, Andrew Armstrong, Daniel George, David Howell, Jiaoti Huang, Michael C. Fitzgerald, Jiyong Hong, Jung Wook Park

    RNA Sequencing:

    Article Title: IL-12-producing iPSC-derived antigen-presenting cells enhance antitumor immunity and enable off-the-shelf immunotherapy.
    Article Snippet: Please cite this article as: Yamamoto M, Zhang R, Kobayashi T, Li Y, Xu H, Mashima H, Saito K, Kariya M, Ide M, Hagiya Y, Fukuda E, Uemura S, Osako Y, Nakaki R, Nakatsura T, Fukushima S, Kaneko S, Hotta A, Ohdan H, Uemura.. Y, IL-12–producing iPSC-derived antigen-presenting cells enhance antitumor immunity and enable off-the-shelf immunotherapy, Molecular Therapy (2026), doi: https:// doi.org/10.1016/j.ymthe.2026.10.017.

    Article Title: Development and Characterization of a Rat Model of Post-traumatic Ankle Osteoarthritis.
    Article Snippet: .. To get sufficient tissue for RNA sequencing, DRGs from 2-3 rats were pooled, and total RNA was extracted using an RNeasy Plus Universal Kit (Qiagen, Germantown, MD) according to the manufacturer's instructions. ..

    Synthesized:

    Article Title: Mesenchymal stromal cells immunosuppress osteoarthritis synovial fluid modulated monocytes via IL-6 and CCL2.
    Article Snippet: .. Total RNA was extracted with RNeasy kit (Qiagen, 73404); cDNA was synthesized using SuperScript IV VILO master mix (ThermoFisher Scientific) per the manufacturer’s instructions. ..



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