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SusTech GmbH arabidopsis rna-seq database
Detection of the CLE2 peptide from <t>Arabidopsis</t> xylem exudate. A, Arabidopsis xylem exudate was collected from cut surfaces of hypocotyls. B, Base peak chromatogram of purified Arabidopsis xylem exudate. C, The MS/MS spectrum of m/z 863.4 eluted at 9.0 min in (B) showed a fragmentation pattern identical to that of arabinosylated CLE2 peptides. B and C, One typical experiment of two replicates is shown. D, Relative mRNA level of the CLE2 gene. Plants grown on sucrose-free or 1% sucrose-containing media were treated with 50-µM DCMU or incubated in darkness for 24 h. The expression levels of each CLE2 gene in the treated samples were normalized to control roots. The dots represent individual measurements. Each result is the mean ± standard error of the mean ( sem ) of measurements obtained from three independent experiments.
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Article Title: Long-distance translocation of CLAVATA3/ESR-related 2 peptide and its positive effect on roots sucrose status

Journal: Plant Physiology

doi: 10.1093/plphys/kiac227

Detection of the CLE2 peptide from Arabidopsis xylem exudate. A, Arabidopsis xylem exudate was collected from cut surfaces of hypocotyls. B, Base peak chromatogram of purified Arabidopsis xylem exudate. C, The MS/MS spectrum of m/z 863.4 eluted at 9.0 min in (B) showed a fragmentation pattern identical to that of arabinosylated CLE2 peptides. B and C, One typical experiment of two replicates is shown. D, Relative mRNA level of the CLE2 gene. Plants grown on sucrose-free or 1% sucrose-containing media were treated with 50-µM DCMU or incubated in darkness for 24 h. The expression levels of each CLE2 gene in the treated samples were normalized to control roots. The dots represent individual measurements. Each result is the mean ± standard error of the mean ( sem ) of measurements obtained from three independent experiments.
Figure Legend Snippet: Detection of the CLE2 peptide from Arabidopsis xylem exudate. A, Arabidopsis xylem exudate was collected from cut surfaces of hypocotyls. B, Base peak chromatogram of purified Arabidopsis xylem exudate. C, The MS/MS spectrum of m/z 863.4 eluted at 9.0 min in (B) showed a fragmentation pattern identical to that of arabinosylated CLE2 peptides. B and C, One typical experiment of two replicates is shown. D, Relative mRNA level of the CLE2 gene. Plants grown on sucrose-free or 1% sucrose-containing media were treated with 50-µM DCMU or incubated in darkness for 24 h. The expression levels of each CLE2 gene in the treated samples were normalized to control roots. The dots represent individual measurements. Each result is the mean ± standard error of the mean ( sem ) of measurements obtained from three independent experiments.

Techniques Used: Purification, Tandem Mass Spectroscopy, Incubation, Expressing, Control

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Article Title: The m6A reader ECT8 is an abiotic stress sensor that accelerates mRNA decay in Arabidopsis.
Article Snippet: 00, No. 0 D ow nloaded from https://academ ic.oup.com /plcell/advance-article/doi/10.1093/plcell/koae149/7687916 by guest on 16 June 2024 For a more comprehensive analysis of its tissue-specific expression pattern, we analyzed RNA-seq data from Arabidopsis RNA-seq database (Zhang et al. 2020) (http://ipf.sustech.edu.cn/ pub/athrna/), which substantiated our findings from GUS staining assays (Supplementary Fig. S3B).

Article Title: A comprehensive characterization and expression profiling of defensin family peptides in Arabidopsis thaliana with a focus on their abiotic stress-specific transcriptional modulation
Article Snippet: For selected defensin family genes, normalized expression levels expressed in fragments per kilobase of transcript per million mapped reads (FPKM) were retrieved from the Arabidopsis RNA-seq database (ARS; http://ipf.sustech.edu.cn/pub/athrna/; June 10, 2023).

Article Title: A comprehensive characterization and expression profiling of defensin family peptides in Arabidopsis thaliana with a focus on their abiotic stress-specific transcriptional modulation
Article Snippet: To this aim, we conducted an in silico gene expression analysis based on data retrieved from the Arabidopsis RNA-seq database (ARS; http://ipf.sustech.edu.cn/pub/athrna/).

Article Title: SERRATE drives phase separation behaviours to regulate m6A modification and miRNA biogenesis.
Article Snippet: The methyltransferase complex (MTC) deposits N6-adenosine (mA) onto RNA, whereas the microprocessor produces microRNA.. Whether and how these two distinct complexes cross-regulate each other has been poorly studied.. Here we report that the MTC subunit B tends to form insoluble condensates with poor activity, with its level monitored by the 20S proteasome.

Article Title: A comprehensive characterization and expression profiling of defensin family peptides in Arabidopsis thaliana with a focus on their abiotic stress-specific transcriptional modulation
Article Snippet: Library numbers, stress types and fold changes for selected up-regulated genes of the defensin family were retrieved from the ’Data Plot’ page of the Arabidopsis RNA-seq database (ARS; http://ipf.sustech.edu.cn/pu b/athrna/; June 10, 2023).

Article Title: The calcium sensor AtCML8 contributes to Arabidopsis plant cell growth by modulating the brassinosteroid signaling pathway
Article Snippet: These data were extracted and formatted using the online expression analysis tool «Arabidopsis RNA-seq Database » (http://ipf.sustech.edu.cn/pub/athrdb/ , Zhang et al, 2020). (C) Representation of CML8 expression levels in different tissues and organs from data integrating single cell RNAseq analyses.

Article Title: Evolution of Whirly1 in the angiosperms: sequence, splicing, and expression in a clade of early transitional mycoheterotrophic orchids
Article Snippet: Additionally, the Klepikova Arabidopsis Atlas ( ) and 1,122 tissue-specific samples available via the Arabidopsis RNA-seq Database ( http://ipf.sustech.edu.cn/pub/athrdb/ accessed 8 March 2023) evidence that WHY1 expression should be expected to be lower in roots or rhizomes than in leaves, which is congruent with expression patterns in C . trifida .

Expressing:

Article Title: Regulation of abscisic acid receptor mRNA stability: Involvement of microRNA5628 in PYL6 transcript decay.
Article Snippet: Phytohormone signaling is fine-tuned by regulatory feedback loops.. The phytohormone abscisic acid (ABA) plays key roles in plant development and abiotic stress tolerance.. PYRABACTIN RESISTENCE 1/PYR1-LIKE/REGULATORY COMPONENT OF ABA RECEPTOR (PYR/PYL/RCAR) receptors sense ABA, and in turn, ABA represses their expression.



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