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rna sequencing (rna-seq) analysis  (Oxford Gene Technology)

 
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    Oxford Gene Technology rna sequencing (rna-seq) analysis
    Differential <t>RNA</t> expression in HSP90i-resistant clones compared to parental Hs578T cells. RNA samples from DMSO-treated and ganetespib-treated Hs578T, CR2 and CR3 cells were analysed for whole transcriptome profiling with <t>RNA-sequencing.</t> Differential gene expression analyses were performed between DMSO-treated parental Hs578T cells with either DMSO-treated CR2 or CR3 and the significantly upregulated genes observed in these clones were mostly overlapping. Pathway enrichment analysis using Metacore™ was performed on the significantly upregulated overlapping genes. The graph represents the top 20 most significantly upregulated pathways in the HSP90i-resistant clones, with FDR (false discovery rate) value < 0.05. Pathways highlighted in blue are linked to JAK-STAT signalling
    Rna Sequencing (Rna Seq) Analysis, supplied by Oxford Gene Technology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna-seq+analysis+and+data+processing/rna+sequencing++rna+seq++library+preparation++high+throughput+sequencing++and+data+analysis/pmc06345040-76-11-15
    Average 90 stars, based on 1 article reviews
    rna sequencing (rna-seq) analysis - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Overcoming acquired resistance to HSP90 inhibition by targeting JAK-STAT signalling in triple-negative breast cancer"

    Article Title: Overcoming acquired resistance to HSP90 inhibition by targeting JAK-STAT signalling in triple-negative breast cancer

    Journal: BMC Cancer

    doi: 10.1186/s12885-019-5295-z

    Differential RNA expression in HSP90i-resistant clones compared to parental Hs578T cells. RNA samples from DMSO-treated and ganetespib-treated Hs578T, CR2 and CR3 cells were analysed for whole transcriptome profiling with RNA-sequencing. Differential gene expression analyses were performed between DMSO-treated parental Hs578T cells with either DMSO-treated CR2 or CR3 and the significantly upregulated genes observed in these clones were mostly overlapping. Pathway enrichment analysis using Metacore™ was performed on the significantly upregulated overlapping genes. The graph represents the top 20 most significantly upregulated pathways in the HSP90i-resistant clones, with FDR (false discovery rate) value < 0.05. Pathways highlighted in blue are linked to JAK-STAT signalling
    Figure Legend Snippet: Differential RNA expression in HSP90i-resistant clones compared to parental Hs578T cells. RNA samples from DMSO-treated and ganetespib-treated Hs578T, CR2 and CR3 cells were analysed for whole transcriptome profiling with RNA-sequencing. Differential gene expression analyses were performed between DMSO-treated parental Hs578T cells with either DMSO-treated CR2 or CR3 and the significantly upregulated genes observed in these clones were mostly overlapping. Pathway enrichment analysis using Metacore™ was performed on the significantly upregulated overlapping genes. The graph represents the top 20 most significantly upregulated pathways in the HSP90i-resistant clones, with FDR (false discovery rate) value < 0.05. Pathways highlighted in blue are linked to JAK-STAT signalling

    Techniques Used: RNA Expression, Clone Assay, RNA Sequencing, Gene Expression

    Related Articles

    RNA Sequencing:

    Article Title: Overcoming acquired resistance to HSP90 inhibition by targeting JAK-STAT signalling in triple-negative breast cancer
    Article Snippet: Duplicate samples (1 μg RNA in 30 μl) were subjected to RNA Sequencing (RNA-seq) analysis (Oxford Gene Technology) and gene expression was quantified using their analysis pipeline [ ].

    Article Title: PEAPOD repressors modulate and coordinate developmental responses to light intensity in Arabidopsis
    Article Snippet: Oxford Gene Technology carried out the RNA‐sequencing (RNA‐seq) library preparation, high‐throughput sequencing, and data analysis.

    Next-Generation Sequencing:

    Article Title: Overcoming acquired resistance to HSP90 inhibition by targeting JAK-STAT signalling in triple-negative breast cancer
    Article Snippet: Duplicate samples (1 μg RNA in 30 μl) were subjected to RNA Sequencing (RNA-seq) analysis (Oxford Gene Technology) and gene expression was quantified using their analysis pipeline [ ].

    Article Title: PEAPOD repressors modulate and coordinate developmental responses to light intensity in Arabidopsis
    Article Snippet: Oxford Gene Technology carried out the RNA‐sequencing (RNA‐seq) library preparation, high‐throughput sequencing, and data analysis.

    Gene Expression:

    Article Title: Overcoming acquired resistance to HSP90 inhibition by targeting JAK-STAT signalling in triple-negative breast cancer
    Article Snippet: Duplicate samples (1 μg RNA in 30 μl) were subjected to RNA Sequencing (RNA-seq) analysis (Oxford Gene Technology) and gene expression was quantified using their analysis pipeline [ ].

    Article Title: PEAPOD repressors modulate and coordinate developmental responses to light intensity in Arabidopsis
    Article Snippet: Oxford Gene Technology carried out the RNA‐sequencing (RNA‐seq) library preparation, high‐throughput sequencing, and data analysis.

    RNA Expression:

    Article Title: Overcoming acquired resistance to HSP90 inhibition by targeting JAK-STAT signalling in triple-negative breast cancer
    Article Snippet: Duplicate samples (1 μg RNA in 30 μl) were subjected to RNA Sequencing (RNA-seq) analysis (Oxford Gene Technology) and gene expression was quantified using their analysis pipeline [ ].

    Article Title: PEAPOD repressors modulate and coordinate developmental responses to light intensity in Arabidopsis
    Article Snippet: Oxford Gene Technology carried out the RNA‐sequencing (RNA‐seq) library preparation, high‐throughput sequencing, and data analysis.

    Clone Assay:

    Article Title: Overcoming acquired resistance to HSP90 inhibition by targeting JAK-STAT signalling in triple-negative breast cancer
    Article Snippet: Duplicate samples (1 μg RNA in 30 μl) were subjected to RNA Sequencing (RNA-seq) analysis (Oxford Gene Technology) and gene expression was quantified using their analysis pipeline [ ].

    Article Title: PEAPOD repressors modulate and coordinate developmental responses to light intensity in Arabidopsis
    Article Snippet: Oxford Gene Technology carried out the RNA‐sequencing (RNA‐seq) library preparation, high‐throughput sequencing, and data analysis.



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