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rna transcriptome sequencing  (Novogene)

 
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    Novogene rna transcriptome sequencing
    FHIT regulates the downstream target KDM6B by mediating its mRNA translation. A , Circos plot displaying Gene Ontology (GO) terms enriched among differentially translated genes identified by Ribo-Seq data. The “LogFC” scale represents the normalized enrichment significance of each pathway (range, 0–1). B , polysome gradient traces from AGS and FHIT-overexpressing AGS cells separated on a 10% to 50% linear sucrose gradient. Western blot analysis confirms FHIT protein expression in both cells. C , RT–qPCR analysis of KDM6B mRNA distribution across polysome fractions in AGS cells with or without FHIT overexpression. mRNA levels were normalized to spiked luciferase <t>RNA</t> and plotted as the percentage of total mRNA in each gradient fraction relative to the input. D – F , RT–qPCR analysis of KDM6B mRNA expression and Western blot detection of KDM6B protein levels in gastric cancer cells or NOC-transformed cells with or without FHIT overexpression. G , Western blot analysis of KDM6B protein levels in NOC-transformed cells (GES-1-MNU) transfected with empty vector (EV) or FHIT-overexpressing constructs after treatment with the transcriptional inhibitor ActD to block nascent mRNA synthesis. The analyses were repeated three times, and the results were expressed as mean ± SD. ns, not significant, ∗ p < 0.05, and ∗∗ p < 0.01. ActD, actinomycin D; FHIT, fragile histidine triad; KDM6B, lysine-specific demethylase 6B; MNU, N -nitroso- N -methylurea; qPCR, quantitative PCR; Ribo-Seq, ribosome <t>sequencing.</t>
    Rna Transcriptome Sequencing, supplied by Novogene, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+transcriptome+sequencing/sequencing+transcriptome/pmc13125197-184-0-10
    Average 86 stars, based on 1 article reviews
    rna transcriptome sequencing - by Bioz Stars, 2026-10
    86/100 stars

    Images

    1) Product Images from "Fragile histidine triad (FHIT) suppresses gastric cancer via translational regulation of the epigenetic modifier KDM6B"

    Article Title: Fragile histidine triad (FHIT) suppresses gastric cancer via translational regulation of the epigenetic modifier KDM6B

    Journal: The Journal of Biological Chemistry

    doi: 10.1016/j.jbc.2026.111404

    FHIT regulates the downstream target KDM6B by mediating its mRNA translation. A , Circos plot displaying Gene Ontology (GO) terms enriched among differentially translated genes identified by Ribo-Seq data. The “LogFC” scale represents the normalized enrichment significance of each pathway (range, 0–1). B , polysome gradient traces from AGS and FHIT-overexpressing AGS cells separated on a 10% to 50% linear sucrose gradient. Western blot analysis confirms FHIT protein expression in both cells. C , RT–qPCR analysis of KDM6B mRNA distribution across polysome fractions in AGS cells with or without FHIT overexpression. mRNA levels were normalized to spiked luciferase RNA and plotted as the percentage of total mRNA in each gradient fraction relative to the input. D – F , RT–qPCR analysis of KDM6B mRNA expression and Western blot detection of KDM6B protein levels in gastric cancer cells or NOC-transformed cells with or without FHIT overexpression. G , Western blot analysis of KDM6B protein levels in NOC-transformed cells (GES-1-MNU) transfected with empty vector (EV) or FHIT-overexpressing constructs after treatment with the transcriptional inhibitor ActD to block nascent mRNA synthesis. The analyses were repeated three times, and the results were expressed as mean ± SD. ns, not significant, ∗ p < 0.05, and ∗∗ p < 0.01. ActD, actinomycin D; FHIT, fragile histidine triad; KDM6B, lysine-specific demethylase 6B; MNU, N -nitroso- N -methylurea; qPCR, quantitative PCR; Ribo-Seq, ribosome sequencing.
    Figure Legend Snippet: FHIT regulates the downstream target KDM6B by mediating its mRNA translation. A , Circos plot displaying Gene Ontology (GO) terms enriched among differentially translated genes identified by Ribo-Seq data. The “LogFC” scale represents the normalized enrichment significance of each pathway (range, 0–1). B , polysome gradient traces from AGS and FHIT-overexpressing AGS cells separated on a 10% to 50% linear sucrose gradient. Western blot analysis confirms FHIT protein expression in both cells. C , RT–qPCR analysis of KDM6B mRNA distribution across polysome fractions in AGS cells with or without FHIT overexpression. mRNA levels were normalized to spiked luciferase RNA and plotted as the percentage of total mRNA in each gradient fraction relative to the input. D – F , RT–qPCR analysis of KDM6B mRNA expression and Western blot detection of KDM6B protein levels in gastric cancer cells or NOC-transformed cells with or without FHIT overexpression. G , Western blot analysis of KDM6B protein levels in NOC-transformed cells (GES-1-MNU) transfected with empty vector (EV) or FHIT-overexpressing constructs after treatment with the transcriptional inhibitor ActD to block nascent mRNA synthesis. The analyses were repeated three times, and the results were expressed as mean ± SD. ns, not significant, ∗ p < 0.05, and ∗∗ p < 0.01. ActD, actinomycin D; FHIT, fragile histidine triad; KDM6B, lysine-specific demethylase 6B; MNU, N -nitroso- N -methylurea; qPCR, quantitative PCR; Ribo-Seq, ribosome sequencing.

    Techniques Used: Western Blot, Expressing, Quantitative RT-PCR, Over Expression, Luciferase, Transformation Assay, Transfection, Plasmid Preparation, Construct, Blocking Assay, Real-time Polymerase Chain Reaction, Sequencing

    Related Articles

    Sequencing:

    Article Title: An astragalus polysaccharide-loaded hydrogel dressing enhances diabetic wound healing via dual-mode electrical stimulation
    Article Snippet: The wound area was quantified using ImageJ software and was determined as a percentage change from initial wound area at day 0. .. The transcriptomic sequencing was performed by the platform of Novogene Co., Ltd, including library construction, high-throughput sequencing, and subsequent analysis (i.e., Differential gene expression analysis, Gene Ontology enrichment analysis, and Gene Set Enrichment Analysis). ..

    Article Title: A Substrate-Dependent Bifunctional Dioxygenase from Fraxinus chinensis for O -Demethylation and C8-Hydroxylation of Coumarins.
    Article Snippet: .. Transcriptome sequencing and bioinformatic analyses were performed by Novogene Co., Ltd. (Beijing, China). .. Restriction enzymes NdeI and HindIII were obtained from New England Biolabs (Ipswich, MA, USA).

    Article Title: Integrative whole-transcriptome analysis of circRNAs, lncRNAs, miRNAs, and mRNAs reveals regulatory networks in mouse brain during Toxoplasma gondii infection.
    Article Snippet: .. We thank Novogene Co., Ltd., China. for technical assistance in Whole transcriptome sequencing. ..

    Article Title: Genome-wide characterisation of the myosin light chain gene family in Chinese perch (Siniperca chuatsi) and its expression patterns in muscle fibre types and injury response.
    Article Snippet: The Class II myosin light chain (myl) genes in Chinese perch (Siniperca chuatsi) have not yet been systematically characterised, and relationships with muscle fibre specification, development, and injury-associated remodelling remain unclear.. In this study, fast and slow muscle fibres were initially distinguished using myofibrillar ATPase histochemistry.. Subsequently, genome-wide mining identified 16 Class II myl genes, comprising eight essential and eight regulatory light-chain subunits.

    Article Title: Designer Dynamic DNA Nanoaggregate in Living Cell for Mitochondrial Energy Restriction.
    Article Snippet: .. The qualified RNA samples were then sent to Novogene Biotech Co., Ltd. (Beijing, China) for transcriptome sequencing. ..

    Next-Generation Sequencing:

    Article Title: An astragalus polysaccharide-loaded hydrogel dressing enhances diabetic wound healing via dual-mode electrical stimulation
    Article Snippet: The wound area was quantified using ImageJ software and was determined as a percentage change from initial wound area at day 0. .. The transcriptomic sequencing was performed by the platform of Novogene Co., Ltd, including library construction, high-throughput sequencing, and subsequent analysis (i.e., Differential gene expression analysis, Gene Ontology enrichment analysis, and Gene Set Enrichment Analysis). ..

    Gene Expression:

    Article Title: An astragalus polysaccharide-loaded hydrogel dressing enhances diabetic wound healing via dual-mode electrical stimulation
    Article Snippet: The wound area was quantified using ImageJ software and was determined as a percentage change from initial wound area at day 0. .. The transcriptomic sequencing was performed by the platform of Novogene Co., Ltd, including library construction, high-throughput sequencing, and subsequent analysis (i.e., Differential gene expression analysis, Gene Ontology enrichment analysis, and Gene Set Enrichment Analysis). ..



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    FHIT regulates the downstream target KDM6B by mediating its mRNA translation. A , Circos plot displaying Gene Ontology (GO) terms enriched among differentially translated genes identified by Ribo-Seq data. The “LogFC” scale represents the normalized enrichment significance of each pathway (range, 0–1). B , polysome gradient traces from AGS and FHIT-overexpressing AGS cells separated on a 10% to 50% linear sucrose gradient. Western blot analysis confirms FHIT protein expression in both cells. C , RT–qPCR analysis of KDM6B mRNA distribution across polysome fractions in AGS cells with or without FHIT overexpression. mRNA levels were normalized to spiked luciferase <t>RNA</t> and plotted as the percentage of total mRNA in each gradient fraction relative to the input. D – F , RT–qPCR analysis of KDM6B mRNA expression and Western blot detection of KDM6B protein levels in gastric cancer cells or NOC-transformed cells with or without FHIT overexpression. G , Western blot analysis of KDM6B protein levels in NOC-transformed cells (GES-1-MNU) transfected with empty vector (EV) or FHIT-overexpressing constructs after treatment with the transcriptional inhibitor ActD to block nascent mRNA synthesis. The analyses were repeated three times, and the results were expressed as mean ± SD. ns, not significant, ∗ p < 0.05, and ∗∗ p < 0.01. ActD, actinomycin D; FHIT, fragile histidine triad; KDM6B, lysine-specific demethylase 6B; MNU, N -nitroso- N -methylurea; qPCR, quantitative PCR; Ribo-Seq, ribosome <t>sequencing.</t>
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    Image Search Results


    FHIT regulates the downstream target KDM6B by mediating its mRNA translation. A , Circos plot displaying Gene Ontology (GO) terms enriched among differentially translated genes identified by Ribo-Seq data. The “LogFC” scale represents the normalized enrichment significance of each pathway (range, 0–1). B , polysome gradient traces from AGS and FHIT-overexpressing AGS cells separated on a 10% to 50% linear sucrose gradient. Western blot analysis confirms FHIT protein expression in both cells. C , RT–qPCR analysis of KDM6B mRNA distribution across polysome fractions in AGS cells with or without FHIT overexpression. mRNA levels were normalized to spiked luciferase RNA and plotted as the percentage of total mRNA in each gradient fraction relative to the input. D – F , RT–qPCR analysis of KDM6B mRNA expression and Western blot detection of KDM6B protein levels in gastric cancer cells or NOC-transformed cells with or without FHIT overexpression. G , Western blot analysis of KDM6B protein levels in NOC-transformed cells (GES-1-MNU) transfected with empty vector (EV) or FHIT-overexpressing constructs after treatment with the transcriptional inhibitor ActD to block nascent mRNA synthesis. The analyses were repeated three times, and the results were expressed as mean ± SD. ns, not significant, ∗ p < 0.05, and ∗∗ p < 0.01. ActD, actinomycin D; FHIT, fragile histidine triad; KDM6B, lysine-specific demethylase 6B; MNU, N -nitroso- N -methylurea; qPCR, quantitative PCR; Ribo-Seq, ribosome sequencing.

    Journal: The Journal of Biological Chemistry

    Article Title: Fragile histidine triad (FHIT) suppresses gastric cancer via translational regulation of the epigenetic modifier KDM6B

    doi: 10.1016/j.jbc.2026.111404

    Figure Lengend Snippet: FHIT regulates the downstream target KDM6B by mediating its mRNA translation. A , Circos plot displaying Gene Ontology (GO) terms enriched among differentially translated genes identified by Ribo-Seq data. The “LogFC” scale represents the normalized enrichment significance of each pathway (range, 0–1). B , polysome gradient traces from AGS and FHIT-overexpressing AGS cells separated on a 10% to 50% linear sucrose gradient. Western blot analysis confirms FHIT protein expression in both cells. C , RT–qPCR analysis of KDM6B mRNA distribution across polysome fractions in AGS cells with or without FHIT overexpression. mRNA levels were normalized to spiked luciferase RNA and plotted as the percentage of total mRNA in each gradient fraction relative to the input. D – F , RT–qPCR analysis of KDM6B mRNA expression and Western blot detection of KDM6B protein levels in gastric cancer cells or NOC-transformed cells with or without FHIT overexpression. G , Western blot analysis of KDM6B protein levels in NOC-transformed cells (GES-1-MNU) transfected with empty vector (EV) or FHIT-overexpressing constructs after treatment with the transcriptional inhibitor ActD to block nascent mRNA synthesis. The analyses were repeated three times, and the results were expressed as mean ± SD. ns, not significant, ∗ p < 0.05, and ∗∗ p < 0.01. ActD, actinomycin D; FHIT, fragile histidine triad; KDM6B, lysine-specific demethylase 6B; MNU, N -nitroso- N -methylurea; qPCR, quantitative PCR; Ribo-Seq, ribosome sequencing.

    Article Snippet: RNA transcriptome sequencing was then performed on each sample by Novogene Corporation.

    Techniques: Western Blot, Expressing, Quantitative RT-PCR, Over Expression, Luciferase, Transformation Assay, Transfection, Plasmid Preparation, Construct, Blocking Assay, Real-time Polymerase Chain Reaction, Sequencing