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high yield t7 biotin16 rna labeling kit  (Jena Bioscience)


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    Structured Review

    Jena Bioscience high yield t7 biotin16 rna labeling kit
    High Yield T7 Biotin16 Rna Labeling Kit, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+transcript+labeling+kit/HighYield+T7+Biotin16+RNA+Labeling+Kit/pmc11535147-177-30-36
    Average 94 stars, based on 2 article reviews
    high yield t7 biotin16 rna labeling kit - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    In Vitro:

    Article Title: NEAT1 promotes genome stability via m 6 A methylation-dependent regulation of CHD4
    Article Snippet: For qualitative analysis, RNA was incubated for 10 min at 75°C in 1 vol of 2× urea dye (7 M urea, 0.05% xylene cyanol, 0.05% bromophenol blue), separated for 30 min at 350 V by 8 M urea-PAGE in TBE buffer (90 mM Tris, 90 mM boric acid, 2 mM EDTA), stained for 20 min in the dark with 1× SYBR Gold (Thermo), and visualized on a transilluminator (Thermo) or analyzed on a fragment analyzer (Agilent). .. To synthesize nonmethylated or biotin-16-UTP-labeled NEAT1 in vitro, 1 μL of 500 ng/μL IVT template or control template (Jena Biosciences) was subjected to IVT for 4 h at 37°C using high-yield T7 biotin16 RNA labeling kit (Jena Biosciences) according to the manufacturer's protocol. .. The scrambled control was transcribed with a SP6 transcription kit (Jena Biosciences) accordingly.

    Article Title: The transcriptional regulator SutA is part of a nutrient scavenging network expressed at the entry to stationary phase in Pseudomonas aeruginosa
    Article Snippet: Short (100-120-bp), complementary regions within each target gene were PCR amplified using Q5 2x Master Mix (New England Biolabs) and primer pairs listed in Table S2, one of which included an overhang containing a T7 class III phi6.5 promoter. .. Randomly Biotin-modified RNA probes were created using Biotin-16-UTP and T7-mediated in vitro transcription using 0.5 μg of PCR as template DNA and the HighYield T7 Biotin16 RNA Labeling Kit (Jena Bioscience, Cat # RNT-101-BIO16) according to the manufacturer’s recommendations. .. Template DNA was removed using the Invitrogen TURBO DNA-free Kit (Thermo Fisher.

    Control:

    Article Title: NEAT1 promotes genome stability via m 6 A methylation-dependent regulation of CHD4
    Article Snippet: For qualitative analysis, RNA was incubated for 10 min at 75°C in 1 vol of 2× urea dye (7 M urea, 0.05% xylene cyanol, 0.05% bromophenol blue), separated for 30 min at 350 V by 8 M urea-PAGE in TBE buffer (90 mM Tris, 90 mM boric acid, 2 mM EDTA), stained for 20 min in the dark with 1× SYBR Gold (Thermo), and visualized on a transilluminator (Thermo) or analyzed on a fragment analyzer (Agilent). .. To synthesize nonmethylated or biotin-16-UTP-labeled NEAT1 in vitro, 1 μL of 500 ng/μL IVT template or control template (Jena Biosciences) was subjected to IVT for 4 h at 37°C using high-yield T7 biotin16 RNA labeling kit (Jena Biosciences) according to the manufacturer's protocol. .. The scrambled control was transcribed with a SP6 transcription kit (Jena Biosciences) accordingly.

    Labeling:

    Article Title: NEAT1 promotes genome stability via m 6 A methylation-dependent regulation of CHD4
    Article Snippet: For qualitative analysis, RNA was incubated for 10 min at 75°C in 1 vol of 2× urea dye (7 M urea, 0.05% xylene cyanol, 0.05% bromophenol blue), separated for 30 min at 350 V by 8 M urea-PAGE in TBE buffer (90 mM Tris, 90 mM boric acid, 2 mM EDTA), stained for 20 min in the dark with 1× SYBR Gold (Thermo), and visualized on a transilluminator (Thermo) or analyzed on a fragment analyzer (Agilent). .. To synthesize nonmethylated or biotin-16-UTP-labeled NEAT1 in vitro, 1 μL of 500 ng/μL IVT template or control template (Jena Biosciences) was subjected to IVT for 4 h at 37°C using high-yield T7 biotin16 RNA labeling kit (Jena Biosciences) according to the manufacturer's protocol. .. The scrambled control was transcribed with a SP6 transcription kit (Jena Biosciences) accordingly.

    Article Title: The transcriptional regulator SutA is part of a nutrient scavenging network expressed at the entry to stationary phase in Pseudomonas aeruginosa
    Article Snippet: Short (100-120-bp), complementary regions within each target gene were PCR amplified using Q5 2x Master Mix (New England Biolabs) and primer pairs listed in Table S2, one of which included an overhang containing a T7 class III phi6.5 promoter. .. Randomly Biotin-modified RNA probes were created using Biotin-16-UTP and T7-mediated in vitro transcription using 0.5 μg of PCR as template DNA and the HighYield T7 Biotin16 RNA Labeling Kit (Jena Bioscience, Cat # RNT-101-BIO16) according to the manufacturer’s recommendations. .. Template DNA was removed using the Invitrogen TURBO DNA-free Kit (Thermo Fisher.

    Polymerase Chain Reaction:

    Article Title: The transcriptional regulator SutA is part of a nutrient scavenging network expressed at the entry to stationary phase in Pseudomonas aeruginosa
    Article Snippet: Short (100-120-bp), complementary regions within each target gene were PCR amplified using Q5 2x Master Mix (New England Biolabs) and primer pairs listed in Table S2, one of which included an overhang containing a T7 class III phi6.5 promoter. .. Randomly Biotin-modified RNA probes were created using Biotin-16-UTP and T7-mediated in vitro transcription using 0.5 μg of PCR as template DNA and the HighYield T7 Biotin16 RNA Labeling Kit (Jena Bioscience, Cat # RNT-101-BIO16) according to the manufacturer’s recommendations. .. Template DNA was removed using the Invitrogen TURBO DNA-free Kit (Thermo Fisher.

    other:

    Article Title: The transcriptional regulator SutA is part of a nutrient scavenging network expressed at the entry to stationary phase in Pseudomonas aeruginosa
    Article Snippet: Plasmids from individual clones were extracted using the Monarch Spin Plasmid Miniprep Kit (New England Biolabs, Cat # T1110) and inserts were Sanger sequenced using primer AS1-fw as sequencing primer.



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