rhil 21 (R&D Systems)
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rhil 21
Rhil 21, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rhil-+21/Recombinant+Human+IL-21+Protein/pmc13089343-228-29-32
Average 94 stars, based on 57 article reviews
Rhil 21, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rhil-+21/Recombinant+Human+IL-21+Protein/pmc13089343-228-29-32
Average 94 stars, based on 57 article reviews
rhil 21 - by Bioz Stars,
2026-11
94/100 stars
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Isolation:Article Title: BCMA/CD19 CAR T cell therapy for refractory myasthenia gravis: Proteomic signatures and single-cell transcriptomics of disease flares. Article Snippet: For AChR- α–specific B cell detection, PBMCs were stained with the CD19 (BioLegend) and AChR- α–loaded dCODE Klickmer and then detected by flow cytometry and laser confocal microscopy (Leica Microsystems). .. B cells (105 per well) isolated from samples collected before CAR T cell therapy and following BCRC were seeded in 96- well plates stimulated with recombinant human CD40L (50 ng/ml; PeproTech), F(ab′)2 fragment goat anti- human IgM (10 μg/ml; Jackson ImmunoResearch) and Article Title: Organ injury in systemic autoimmunity is mediated by stem-like CD8 + T cells arising from tissue-draining lymph nodes. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PE anti-mouse IL-21R Antibody Biolegend Cat#131906; RRID:AB_1279430 BD PharmingenTM Purified NA/LE Hamster Anti-Mouse CD3e BD Biosciences Cat#553057; RRID:AB_394590 BD PharmingenTM Purified NA/LE Mouse Anti-Mouse CD28 BD Biosciences Cat#566883; RRID:AB_2869934 Ultra-LEAFTM Purified anti-mouse CD8a Antibody Biolegend Cat#100764; RRID:AB_2810324 Ultra-LEAFTM Purified Rat IgG2a, κ Isotype Ctrl Antibody Biolegend Cat#400566; RRID:AB_11148951 TotalSeqTM-C0301 anti-mouse Hashtag 1 Antibody Biolegend Cat#155861; RRID:AB_2800693 TotalSeqTM-C0302 anti-mouse Hashtag 2 Antibody Biolegend Cat#155863; RRID:AB_2800694 TotalSeqTM-C0303 anti-mouse Hashtag 3 Antibody Biolegend Cat#155865; RRID:AB_2800695 InVivoMAb rat IgG2a isotype control, antitrinitrophenol BioXCell Cat#BE0089; RRID:AB_1107769 InVivoMAb anti-mouse CD122 (IL-2Rβ) BioXCell Cat#BE0272; RRID:AB_2687795 InVivoMAb anti-mouse IL-21R BioXCell Cat#BE0258; RRID:AB_2687737 InVivoMAb anti-mouse PD-1 (CD279) BioXCell Cat#BE0273; RRID:AB_2687796 CD3 Monoclonal Antibody (OKT3), Functional Grade, eBioscienceTM Invitrogen Cat#16-0037-85; RRID:AB_468855 CD28 Monoclonal Antibody (CD28.2), Functional Grade, eBioscienceTM Invitrogen Cat#16-0289-85; RRID:AB_468927 anti-Nephrin guinea pig polyclonal, serum PROGEN Cat#GP-N2; RRID:AB_2904121 Complement C3 Polyclonal Antibody Invitrogen Cat#PA5-21349; RRID:AB_11153785 APC Goat anti-mouse IgG (minimal x-reactivity) Antibody Biolegend Cat#405308; RRID:AB_315011 Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 488) Abcam Cat#ab150185; RRID:AB_2736871 Goat Anti-Mouse IgG, Human ads-AP SouthernBiotech Cat#1030-04; RRID:AB_2794293 Biological samples Lupus patient PBMCs Yale University N/A Chemicals, peptides, and recombinant proteins LIVE/DEADTM Fixable Aqua Dead Cell Stain Kit Invitrogen Cat#L34966 Recombinant Mouse IFN-gamma Protein with carrier R&D Systems Cat#485-MI-100 Recombinant Mouse TNF-alpha (aa 80-235) Protein with carrier R&D Systems Cat#410-MT-025 Recombinant Mouse IL-10 Protein with carrier R&D Systems Cat#417-ML-025 Recombinant Mouse IL-12 Protein with carrier R&D Systems Cat#419-ML-010 Recombinant Mouse IL-15 Protein with carrier R&D Systems Ca#447-ML-010 Recombinant Mouse IL-18/IL-1F4 Protein with carrier R&D Systems Cat#9139-IL-050 Recombinant Mouse IL-21 Protein with carrier R&D Systems Cat#594-ML-025 Universal Type I IFN Protein R&D Systems Cat#11200-1 Human IL-2 Recombinant Protein PeproTech Cat#200-02 (Continued on next page) ll OPEN ACCESSArticle Immunity 59, 1–21.e1–e15, June 9, 2026 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant Human IL-15 Protein, CF R&D Systems Cat#BT-015-010 Recombinant:Article Title: BCMA/CD19 CAR T cell therapy for refractory myasthenia gravis: Proteomic signatures and single-cell transcriptomics of disease flares. Article Snippet: For AChR- α–specific B cell detection, PBMCs were stained with the CD19 (BioLegend) and AChR- α–loaded dCODE Klickmer and then detected by flow cytometry and laser confocal microscopy (Leica Microsystems). .. B cells (105 per well) isolated from samples collected before CAR T cell therapy and following BCRC were seeded in 96- well plates stimulated with recombinant human CD40L (50 ng/ml; PeproTech), F(ab′)2 fragment goat anti- human IgM (10 μg/ml; Jackson ImmunoResearch) and Article Title: Organ injury in systemic autoimmunity is mediated by stem-like CD8 + T cells arising from tissue-draining lymph nodes. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PE anti-mouse IL-21R Antibody Biolegend Cat#131906; RRID:AB_1279430 BD PharmingenTM Purified NA/LE Hamster Anti-Mouse CD3e BD Biosciences Cat#553057; RRID:AB_394590 BD PharmingenTM Purified NA/LE Mouse Anti-Mouse CD28 BD Biosciences Cat#566883; RRID:AB_2869934 Ultra-LEAFTM Purified anti-mouse CD8a Antibody Biolegend Cat#100764; RRID:AB_2810324 Ultra-LEAFTM Purified Rat IgG2a, κ Isotype Ctrl Antibody Biolegend Cat#400566; RRID:AB_11148951 TotalSeqTM-C0301 anti-mouse Hashtag 1 Antibody Biolegend Cat#155861; RRID:AB_2800693 TotalSeqTM-C0302 anti-mouse Hashtag 2 Antibody Biolegend Cat#155863; RRID:AB_2800694 TotalSeqTM-C0303 anti-mouse Hashtag 3 Antibody Biolegend Cat#155865; RRID:AB_2800695 InVivoMAb rat IgG2a isotype control, antitrinitrophenol BioXCell Cat#BE0089; RRID:AB_1107769 InVivoMAb anti-mouse CD122 (IL-2Rβ) BioXCell Cat#BE0272; RRID:AB_2687795 InVivoMAb anti-mouse IL-21R BioXCell Cat#BE0258; RRID:AB_2687737 InVivoMAb anti-mouse PD-1 (CD279) BioXCell Cat#BE0273; RRID:AB_2687796 CD3 Monoclonal Antibody (OKT3), Functional Grade, eBioscienceTM Invitrogen Cat#16-0037-85; RRID:AB_468855 CD28 Monoclonal Antibody (CD28.2), Functional Grade, eBioscienceTM Invitrogen Cat#16-0289-85; RRID:AB_468927 anti-Nephrin guinea pig polyclonal, serum PROGEN Cat#GP-N2; RRID:AB_2904121 Complement C3 Polyclonal Antibody Invitrogen Cat#PA5-21349; RRID:AB_11153785 APC Goat anti-mouse IgG (minimal x-reactivity) Antibody Biolegend Cat#405308; RRID:AB_315011 Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 488) Abcam Cat#ab150185; RRID:AB_2736871 Goat Anti-Mouse IgG, Human ads-AP SouthernBiotech Cat#1030-04; RRID:AB_2794293 Biological samples Lupus patient PBMCs Yale University N/A Chemicals, peptides, and recombinant proteins LIVE/DEADTM Fixable Aqua Dead Cell Stain Kit Invitrogen Cat#L34966 Recombinant Mouse IFN-gamma Protein with carrier R&D Systems Cat#485-MI-100 Recombinant Mouse TNF-alpha (aa 80-235) Protein with carrier R&D Systems Cat#410-MT-025 Recombinant Mouse IL-10 Protein with carrier R&D Systems Cat#417-ML-025 Recombinant Mouse IL-12 Protein with carrier R&D Systems Cat#419-ML-010 Recombinant Mouse IL-15 Protein with carrier R&D Systems Ca#447-ML-010 Recombinant Mouse IL-18/IL-1F4 Protein with carrier R&D Systems Cat#9139-IL-050 Recombinant Mouse IL-21 Protein with carrier R&D Systems Cat#594-ML-025 Universal Type I IFN Protein R&D Systems Cat#11200-1 Human IL-2 Recombinant Protein PeproTech Cat#200-02 (Continued on next page) ll OPEN ACCESSArticle Immunity 59, 1–21.e1–e15, June 9, 2026 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant Human IL-15 Protein, CF R&D Systems Cat#BT-015-010 Article Title: A Feeder Cell-Free System for Chimeric Antigen Receptor Gene Transduction Into Natural Killer Cells Article Snippet: Recombinant human IL-18 (R&D Systems, catalog number: 9124-IL) 10. .. Article Title: N-glycosylation is essential for aberrant Tfh cells differentiation in systemic lupus erythematosus. Article Snippet: Aberrant differentiation of T follicular helper (Tfh) cells contributes to the pathogenesis of autoimmune diseases such as systemic lupus erythematosus (SLE).. However, whether Tfh cell differentiation is regulated by glycosylation remains unclear.. In this study, we found that inhibition of N-glycosylation completely blocked Tfh cell differentiation. Immunopeptidomics:Article Title: Healthy donor T cell receptors expand functional neoantigen recognition beyond patient vaccination Article Snippet: For patients, pan CD8 + T cells were isolated using the CD8 + T cell Isolation Kit (Miltenyi Biotec, #130-096-495). .. Matured MoDCs were cocultured with the respective CD8 + T cells at a 1:4 ratio (MoDC:CD8 + T cell) in RPMI 1640 with 10% human serum (HS), 1% PS, rhIL-21 (30 ng/ml; R&D Systems, 8879-IL) for 3 days in Biomarker Discovery:Article Title: Healthy donor T cell receptors expand functional neoantigen recognition beyond patient vaccination Article Snippet: For patients, pan CD8 + T cells were isolated using the CD8 + T cell Isolation Kit (Miltenyi Biotec, #130-096-495). .. Matured MoDCs were cocultured with the respective CD8 + T cells at a 1:4 ratio (MoDC:CD8 + T cell) in RPMI 1640 with 10% human serum (HS), 1% PS, rhIL-21 (30 ng/ml; R&D Systems, 8879-IL) for 3 days in Single Cell:Article Title: Organ injury in systemic autoimmunity is mediated by stem-like CD8 + T cells arising from tissue-draining lymph nodes. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PE anti-mouse IL-21R Antibody Biolegend Cat#131906; RRID:AB_1279430 BD PharmingenTM Purified NA/LE Hamster Anti-Mouse CD3e BD Biosciences Cat#553057; RRID:AB_394590 BD PharmingenTM Purified NA/LE Mouse Anti-Mouse CD28 BD Biosciences Cat#566883; RRID:AB_2869934 Ultra-LEAFTM Purified anti-mouse CD8a Antibody Biolegend Cat#100764; RRID:AB_2810324 Ultra-LEAFTM Purified Rat IgG2a, κ Isotype Ctrl Antibody Biolegend Cat#400566; RRID:AB_11148951 TotalSeqTM-C0301 anti-mouse Hashtag 1 Antibody Biolegend Cat#155861; RRID:AB_2800693 TotalSeqTM-C0302 anti-mouse Hashtag 2 Antibody Biolegend Cat#155863; RRID:AB_2800694 TotalSeqTM-C0303 anti-mouse Hashtag 3 Antibody Biolegend Cat#155865; RRID:AB_2800695 InVivoMAb rat IgG2a isotype control, antitrinitrophenol BioXCell Cat#BE0089; RRID:AB_1107769 InVivoMAb anti-mouse CD122 (IL-2Rβ) BioXCell Cat#BE0272; RRID:AB_2687795 InVivoMAb anti-mouse IL-21R BioXCell Cat#BE0258; RRID:AB_2687737 InVivoMAb anti-mouse PD-1 (CD279) BioXCell Cat#BE0273; RRID:AB_2687796 CD3 Monoclonal Antibody (OKT3), Functional Grade, eBioscienceTM Invitrogen Cat#16-0037-85; RRID:AB_468855 CD28 Monoclonal Antibody (CD28.2), Functional Grade, eBioscienceTM Invitrogen Cat#16-0289-85; RRID:AB_468927 anti-Nephrin guinea pig polyclonal, serum PROGEN Cat#GP-N2; RRID:AB_2904121 Complement C3 Polyclonal Antibody Invitrogen Cat#PA5-21349; RRID:AB_11153785 APC Goat anti-mouse IgG (minimal x-reactivity) Antibody Biolegend Cat#405308; RRID:AB_315011 Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 488) Abcam Cat#ab150185; RRID:AB_2736871 Goat Anti-Mouse IgG, Human ads-AP SouthernBiotech Cat#1030-04; RRID:AB_2794293 Biological samples Lupus patient PBMCs Yale University N/A Chemicals, peptides, and recombinant proteins LIVE/DEADTM Fixable Aqua Dead Cell Stain Kit Invitrogen Cat#L34966 Recombinant Mouse IFN-gamma Protein with carrier R&D Systems Cat#485-MI-100 Recombinant Mouse TNF-alpha (aa 80-235) Protein with carrier R&D Systems Cat#410-MT-025 Recombinant Mouse IL-10 Protein with carrier R&D Systems Cat#417-ML-025 Recombinant Mouse IL-12 Protein with carrier R&D Systems Cat#419-ML-010 Recombinant Mouse IL-15 Protein with carrier R&D Systems Ca#447-ML-010 Recombinant Mouse IL-18/IL-1F4 Protein with carrier R&D Systems Cat#9139-IL-050 Recombinant Mouse IL-21 Protein with carrier R&D Systems Cat#594-ML-025 Universal Type I IFN Protein R&D Systems Cat#11200-1 Human IL-2 Recombinant Protein PeproTech Cat#200-02 (Continued on next page) ll OPEN ACCESSArticle Immunity 59, 1–21.e1–e15, June 9, 2026 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant Human IL-15 Protein, CF R&D Systems Cat#BT-015-010 Gene Expression:Article Title: Organ injury in systemic autoimmunity is mediated by stem-like CD8 + T cells arising from tissue-draining lymph nodes. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PE anti-mouse IL-21R Antibody Biolegend Cat#131906; RRID:AB_1279430 BD PharmingenTM Purified NA/LE Hamster Anti-Mouse CD3e BD Biosciences Cat#553057; RRID:AB_394590 BD PharmingenTM Purified NA/LE Mouse Anti-Mouse CD28 BD Biosciences Cat#566883; RRID:AB_2869934 Ultra-LEAFTM Purified anti-mouse CD8a Antibody Biolegend Cat#100764; RRID:AB_2810324 Ultra-LEAFTM Purified Rat IgG2a, κ Isotype Ctrl Antibody Biolegend Cat#400566; RRID:AB_11148951 TotalSeqTM-C0301 anti-mouse Hashtag 1 Antibody Biolegend Cat#155861; RRID:AB_2800693 TotalSeqTM-C0302 anti-mouse Hashtag 2 Antibody Biolegend Cat#155863; RRID:AB_2800694 TotalSeqTM-C0303 anti-mouse Hashtag 3 Antibody Biolegend Cat#155865; RRID:AB_2800695 InVivoMAb rat IgG2a isotype control, antitrinitrophenol BioXCell Cat#BE0089; RRID:AB_1107769 InVivoMAb anti-mouse CD122 (IL-2Rβ) BioXCell Cat#BE0272; RRID:AB_2687795 InVivoMAb anti-mouse IL-21R BioXCell Cat#BE0258; RRID:AB_2687737 InVivoMAb anti-mouse PD-1 (CD279) BioXCell Cat#BE0273; RRID:AB_2687796 CD3 Monoclonal Antibody (OKT3), Functional Grade, eBioscienceTM Invitrogen Cat#16-0037-85; RRID:AB_468855 CD28 Monoclonal Antibody (CD28.2), Functional Grade, eBioscienceTM Invitrogen Cat#16-0289-85; RRID:AB_468927 anti-Nephrin guinea pig polyclonal, serum PROGEN Cat#GP-N2; RRID:AB_2904121 Complement C3 Polyclonal Antibody Invitrogen Cat#PA5-21349; RRID:AB_11153785 APC Goat anti-mouse IgG (minimal x-reactivity) Antibody Biolegend Cat#405308; RRID:AB_315011 Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 488) Abcam Cat#ab150185; RRID:AB_2736871 Goat Anti-Mouse IgG, Human ads-AP SouthernBiotech Cat#1030-04; RRID:AB_2794293 Biological samples Lupus patient PBMCs Yale University N/A Chemicals, peptides, and recombinant proteins LIVE/DEADTM Fixable Aqua Dead Cell Stain Kit Invitrogen Cat#L34966 Recombinant Mouse IFN-gamma Protein with carrier R&D Systems Cat#485-MI-100 Recombinant Mouse TNF-alpha (aa 80-235) Protein with carrier R&D Systems Cat#410-MT-025 Recombinant Mouse IL-10 Protein with carrier R&D Systems Cat#417-ML-025 Recombinant Mouse IL-12 Protein with carrier R&D Systems Cat#419-ML-010 Recombinant Mouse IL-15 Protein with carrier R&D Systems Ca#447-ML-010 Recombinant Mouse IL-18/IL-1F4 Protein with carrier R&D Systems Cat#9139-IL-050 Recombinant Mouse IL-21 Protein with carrier R&D Systems Cat#594-ML-025 Universal Type I IFN Protein R&D Systems Cat#11200-1 Human IL-2 Recombinant Protein PeproTech Cat#200-02 (Continued on next page) ll OPEN ACCESSArticle Immunity 59, 1–21.e1–e15, June 9, 2026 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant Human IL-15 Protein, CF R&D Systems Cat#BT-015-010 Cell Isolation:Article Title: Organ injury in systemic autoimmunity is mediated by stem-like CD8 + T cells arising from tissue-draining lymph nodes. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PE anti-mouse IL-21R Antibody Biolegend Cat#131906; RRID:AB_1279430 BD PharmingenTM Purified NA/LE Hamster Anti-Mouse CD3e BD Biosciences Cat#553057; RRID:AB_394590 BD PharmingenTM Purified NA/LE Mouse Anti-Mouse CD28 BD Biosciences Cat#566883; RRID:AB_2869934 Ultra-LEAFTM Purified anti-mouse CD8a Antibody Biolegend Cat#100764; RRID:AB_2810324 Ultra-LEAFTM Purified Rat IgG2a, κ Isotype Ctrl Antibody Biolegend Cat#400566; RRID:AB_11148951 TotalSeqTM-C0301 anti-mouse Hashtag 1 Antibody Biolegend Cat#155861; RRID:AB_2800693 TotalSeqTM-C0302 anti-mouse Hashtag 2 Antibody Biolegend Cat#155863; RRID:AB_2800694 TotalSeqTM-C0303 anti-mouse Hashtag 3 Antibody Biolegend Cat#155865; RRID:AB_2800695 InVivoMAb rat IgG2a isotype control, antitrinitrophenol BioXCell Cat#BE0089; RRID:AB_1107769 InVivoMAb anti-mouse CD122 (IL-2Rβ) BioXCell Cat#BE0272; RRID:AB_2687795 InVivoMAb anti-mouse IL-21R BioXCell Cat#BE0258; RRID:AB_2687737 InVivoMAb anti-mouse PD-1 (CD279) BioXCell Cat#BE0273; RRID:AB_2687796 CD3 Monoclonal Antibody (OKT3), Functional Grade, eBioscienceTM Invitrogen Cat#16-0037-85; RRID:AB_468855 CD28 Monoclonal Antibody (CD28.2), Functional Grade, eBioscienceTM Invitrogen Cat#16-0289-85; RRID:AB_468927 anti-Nephrin guinea pig polyclonal, serum PROGEN Cat#GP-N2; RRID:AB_2904121 Complement C3 Polyclonal Antibody Invitrogen Cat#PA5-21349; RRID:AB_11153785 APC Goat anti-mouse IgG (minimal x-reactivity) Antibody Biolegend Cat#405308; RRID:AB_315011 Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 488) Abcam Cat#ab150185; RRID:AB_2736871 Goat Anti-Mouse IgG, Human ads-AP SouthernBiotech Cat#1030-04; RRID:AB_2794293 Biological samples Lupus patient PBMCs Yale University N/A Chemicals, peptides, and recombinant proteins LIVE/DEADTM Fixable Aqua Dead Cell Stain Kit Invitrogen Cat#L34966 Recombinant Mouse IFN-gamma Protein with carrier R&D Systems Cat#485-MI-100 Recombinant Mouse TNF-alpha (aa 80-235) Protein with carrier R&D Systems Cat#410-MT-025 Recombinant Mouse IL-10 Protein with carrier R&D Systems Cat#417-ML-025 Recombinant Mouse IL-12 Protein with carrier R&D Systems Cat#419-ML-010 Recombinant Mouse IL-15 Protein with carrier R&D Systems Ca#447-ML-010 Recombinant Mouse IL-18/IL-1F4 Protein with carrier R&D Systems Cat#9139-IL-050 Recombinant Mouse IL-21 Protein with carrier R&D Systems Cat#594-ML-025 Universal Type I IFN Protein R&D Systems Cat#11200-1 Human IL-2 Recombinant Protein PeproTech Cat#200-02 (Continued on next page) ll OPEN ACCESSArticle Immunity 59, 1–21.e1–e15, June 9, 2026 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant Human IL-15 Protein, CF R&D Systems Cat#BT-015-010 Bradford Protein Assay:Article Title: Organ injury in systemic autoimmunity is mediated by stem-like CD8 + T cells arising from tissue-draining lymph nodes. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PE anti-mouse IL-21R Antibody Biolegend Cat#131906; RRID:AB_1279430 BD PharmingenTM Purified NA/LE Hamster Anti-Mouse CD3e BD Biosciences Cat#553057; RRID:AB_394590 BD PharmingenTM Purified NA/LE Mouse Anti-Mouse CD28 BD Biosciences Cat#566883; RRID:AB_2869934 Ultra-LEAFTM Purified anti-mouse CD8a Antibody Biolegend Cat#100764; RRID:AB_2810324 Ultra-LEAFTM Purified Rat IgG2a, κ Isotype Ctrl Antibody Biolegend Cat#400566; RRID:AB_11148951 TotalSeqTM-C0301 anti-mouse Hashtag 1 Antibody Biolegend Cat#155861; RRID:AB_2800693 TotalSeqTM-C0302 anti-mouse Hashtag 2 Antibody Biolegend Cat#155863; RRID:AB_2800694 TotalSeqTM-C0303 anti-mouse Hashtag 3 Antibody Biolegend Cat#155865; RRID:AB_2800695 InVivoMAb rat IgG2a isotype control, antitrinitrophenol BioXCell Cat#BE0089; RRID:AB_1107769 InVivoMAb anti-mouse CD122 (IL-2Rβ) BioXCell Cat#BE0272; RRID:AB_2687795 InVivoMAb anti-mouse IL-21R BioXCell Cat#BE0258; RRID:AB_2687737 InVivoMAb anti-mouse PD-1 (CD279) BioXCell Cat#BE0273; RRID:AB_2687796 CD3 Monoclonal Antibody (OKT3), Functional Grade, eBioscienceTM Invitrogen Cat#16-0037-85; RRID:AB_468855 CD28 Monoclonal Antibody (CD28.2), Functional Grade, eBioscienceTM Invitrogen Cat#16-0289-85; RRID:AB_468927 anti-Nephrin guinea pig polyclonal, serum PROGEN Cat#GP-N2; RRID:AB_2904121 Complement C3 Polyclonal Antibody Invitrogen Cat#PA5-21349; RRID:AB_11153785 APC Goat anti-mouse IgG (minimal x-reactivity) Antibody Biolegend Cat#405308; RRID:AB_315011 Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 488) Abcam Cat#ab150185; RRID:AB_2736871 Goat Anti-Mouse IgG, Human ads-AP SouthernBiotech Cat#1030-04; RRID:AB_2794293 Biological samples Lupus patient PBMCs Yale University N/A Chemicals, peptides, and recombinant proteins LIVE/DEADTM Fixable Aqua Dead Cell Stain Kit Invitrogen Cat#L34966 Recombinant Mouse IFN-gamma Protein with carrier R&D Systems Cat#485-MI-100 Recombinant Mouse TNF-alpha (aa 80-235) Protein with carrier R&D Systems Cat#410-MT-025 Recombinant Mouse IL-10 Protein with carrier R&D Systems Cat#417-ML-025 Recombinant Mouse IL-12 Protein with carrier R&D Systems Cat#419-ML-010 Recombinant Mouse IL-15 Protein with carrier R&D Systems Ca#447-ML-010 Recombinant Mouse IL-18/IL-1F4 Protein with carrier R&D Systems Cat#9139-IL-050 Recombinant Mouse IL-21 Protein with carrier R&D Systems Cat#594-ML-025 Universal Type I IFN Protein R&D Systems Cat#11200-1 Human IL-2 Recombinant Protein PeproTech Cat#200-02 (Continued on next page) ll OPEN ACCESSArticle Immunity 59, 1–21.e1–e15, June 9, 2026 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant Human IL-15 Protein, CF R&D Systems Cat#BT-015-010 Sequencing:Article Title: Organ injury in systemic autoimmunity is mediated by stem-like CD8 + T cells arising from tissue-draining lymph nodes. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PE anti-mouse IL-21R Antibody Biolegend Cat#131906; RRID:AB_1279430 BD PharmingenTM Purified NA/LE Hamster Anti-Mouse CD3e BD Biosciences Cat#553057; RRID:AB_394590 BD PharmingenTM Purified NA/LE Mouse Anti-Mouse CD28 BD Biosciences Cat#566883; RRID:AB_2869934 Ultra-LEAFTM Purified anti-mouse CD8a Antibody Biolegend Cat#100764; RRID:AB_2810324 Ultra-LEAFTM Purified Rat IgG2a, κ Isotype Ctrl Antibody Biolegend Cat#400566; RRID:AB_11148951 TotalSeqTM-C0301 anti-mouse Hashtag 1 Antibody Biolegend Cat#155861; RRID:AB_2800693 TotalSeqTM-C0302 anti-mouse Hashtag 2 Antibody Biolegend Cat#155863; RRID:AB_2800694 TotalSeqTM-C0303 anti-mouse Hashtag 3 Antibody Biolegend Cat#155865; RRID:AB_2800695 InVivoMAb rat IgG2a isotype control, antitrinitrophenol BioXCell Cat#BE0089; RRID:AB_1107769 InVivoMAb anti-mouse CD122 (IL-2Rβ) BioXCell Cat#BE0272; RRID:AB_2687795 InVivoMAb anti-mouse IL-21R BioXCell Cat#BE0258; RRID:AB_2687737 InVivoMAb anti-mouse PD-1 (CD279) BioXCell Cat#BE0273; RRID:AB_2687796 CD3 Monoclonal Antibody (OKT3), Functional Grade, eBioscienceTM Invitrogen Cat#16-0037-85; RRID:AB_468855 CD28 Monoclonal Antibody (CD28.2), Functional Grade, eBioscienceTM Invitrogen Cat#16-0289-85; RRID:AB_468927 anti-Nephrin guinea pig polyclonal, serum PROGEN Cat#GP-N2; RRID:AB_2904121 Complement C3 Polyclonal Antibody Invitrogen Cat#PA5-21349; RRID:AB_11153785 APC Goat anti-mouse IgG (minimal x-reactivity) Antibody Biolegend Cat#405308; RRID:AB_315011 Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 488) Abcam Cat#ab150185; RRID:AB_2736871 Goat Anti-Mouse IgG, Human ads-AP SouthernBiotech Cat#1030-04; RRID:AB_2794293 Biological samples Lupus patient PBMCs Yale University N/A Chemicals, peptides, and recombinant proteins LIVE/DEADTM Fixable Aqua Dead Cell Stain Kit Invitrogen Cat#L34966 Recombinant Mouse IFN-gamma Protein with carrier R&D Systems Cat#485-MI-100 Recombinant Mouse TNF-alpha (aa 80-235) Protein with carrier R&D Systems Cat#410-MT-025 Recombinant Mouse IL-10 Protein with carrier R&D Systems Cat#417-ML-025 Recombinant Mouse IL-12 Protein with carrier R&D Systems Cat#419-ML-010 Recombinant Mouse IL-15 Protein with carrier R&D Systems Ca#447-ML-010 Recombinant Mouse IL-18/IL-1F4 Protein with carrier R&D Systems Cat#9139-IL-050 Recombinant Mouse IL-21 Protein with carrier R&D Systems Cat#594-ML-025 Universal Type I IFN Protein R&D Systems Cat#11200-1 Human IL-2 Recombinant Protein PeproTech Cat#200-02 (Continued on next page) ll OPEN ACCESSArticle Immunity 59, 1–21.e1–e15, June 9, 2026 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant Human IL-15 Protein, CF R&D Systems Cat#BT-015-010 Software:Article Title: Organ injury in systemic autoimmunity is mediated by stem-like CD8 + T cells arising from tissue-draining lymph nodes. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PE anti-mouse IL-21R Antibody Biolegend Cat#131906; RRID:AB_1279430 BD PharmingenTM Purified NA/LE Hamster Anti-Mouse CD3e BD Biosciences Cat#553057; RRID:AB_394590 BD PharmingenTM Purified NA/LE Mouse Anti-Mouse CD28 BD Biosciences Cat#566883; RRID:AB_2869934 Ultra-LEAFTM Purified anti-mouse CD8a Antibody Biolegend Cat#100764; RRID:AB_2810324 Ultra-LEAFTM Purified Rat IgG2a, κ Isotype Ctrl Antibody Biolegend Cat#400566; RRID:AB_11148951 TotalSeqTM-C0301 anti-mouse Hashtag 1 Antibody Biolegend Cat#155861; RRID:AB_2800693 TotalSeqTM-C0302 anti-mouse Hashtag 2 Antibody Biolegend Cat#155863; RRID:AB_2800694 TotalSeqTM-C0303 anti-mouse Hashtag 3 Antibody Biolegend Cat#155865; RRID:AB_2800695 InVivoMAb rat IgG2a isotype control, antitrinitrophenol BioXCell Cat#BE0089; RRID:AB_1107769 InVivoMAb anti-mouse CD122 (IL-2Rβ) BioXCell Cat#BE0272; RRID:AB_2687795 InVivoMAb anti-mouse IL-21R BioXCell Cat#BE0258; RRID:AB_2687737 InVivoMAb anti-mouse PD-1 (CD279) BioXCell Cat#BE0273; RRID:AB_2687796 CD3 Monoclonal Antibody (OKT3), Functional Grade, eBioscienceTM Invitrogen Cat#16-0037-85; RRID:AB_468855 CD28 Monoclonal Antibody (CD28.2), Functional Grade, eBioscienceTM Invitrogen Cat#16-0289-85; RRID:AB_468927 anti-Nephrin guinea pig polyclonal, serum PROGEN Cat#GP-N2; RRID:AB_2904121 Complement C3 Polyclonal Antibody Invitrogen Cat#PA5-21349; RRID:AB_11153785 APC Goat anti-mouse IgG (minimal x-reactivity) Antibody Biolegend Cat#405308; RRID:AB_315011 Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 488) Abcam Cat#ab150185; RRID:AB_2736871 Goat Anti-Mouse IgG, Human ads-AP SouthernBiotech Cat#1030-04; RRID:AB_2794293 Biological samples Lupus patient PBMCs Yale University N/A Chemicals, peptides, and recombinant proteins LIVE/DEADTM Fixable Aqua Dead Cell Stain Kit Invitrogen Cat#L34966 Recombinant Mouse IFN-gamma Protein with carrier R&D Systems Cat#485-MI-100 Recombinant Mouse TNF-alpha (aa 80-235) Protein with carrier R&D Systems Cat#410-MT-025 Recombinant Mouse IL-10 Protein with carrier R&D Systems Cat#417-ML-025 Recombinant Mouse IL-12 Protein with carrier R&D Systems Cat#419-ML-010 Recombinant Mouse IL-15 Protein with carrier R&D Systems Ca#447-ML-010 Recombinant Mouse IL-18/IL-1F4 Protein with carrier R&D Systems Cat#9139-IL-050 Recombinant Mouse IL-21 Protein with carrier R&D Systems Cat#594-ML-025 Universal Type I IFN Protein R&D Systems Cat#11200-1 Human IL-2 Recombinant Protein PeproTech Cat#200-02 (Continued on next page) ll OPEN ACCESSArticle Immunity 59, 1–21.e1–e15, June 9, 2026 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant Human IL-15 Protein, CF R&D Systems Cat#BT-015-010 |