438 c baculovirus expression vector (Addgene inc)
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438 c baculovirus expression vector
438 C Baculovirus Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rgfp/pFastBac+StrepII+msfGFP+TEV+cloning+vector+with+BioBrick+PolyPromoter+LIC+Subcloning+(438-Rgfp)+(Plasmid+%2355221)/pmc12001331-533-8-12
Average 92 stars, based on 8 article reviews
438 C Baculovirus Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rgfp/pFastBac+StrepII+msfGFP+TEV+cloning+vector+with+BioBrick+PolyPromoter+LIC+Subcloning+(438-Rgfp)+(Plasmid+%2355221)/pmc12001331-533-8-12
Average 92 stars, based on 8 article reviews
438 c baculovirus expression vector - by Bioz Stars,
2026-11
92/100 stars
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Plasmid Preparation:Article Title: Molecular architecture of the human tRNA ligase complex Article Snippet: DNA encoding human RTCB (UniProt Q9Y3I0) and CGI-99 (UniProt Q9Y224) was each inserted into the UC Berkeley MacroLab 438A vector (gift from Scott Gradia, Addgene plasmid #55218) using ligation-independent cloning. .. DDX1 (UniProt Q92499), FAM98B (UniProt Q52LJ0), and ASW (UniProt Q9BVC5-1) were subcloned into the UC Berkeley MacroLab plasmids 438B (Addgene plasmid #55219), Article Title: ARF alters PAF1 complex integrity to selectively repress oncogenic transcription programs upon p53 loss Article Snippet: Beads were washed 4 times with 10 mL Strep wash buffer (20 mM Tris-HCl pH 8.0, 300 mM NaCl, 10% glycerol, 1% NP-40, 1 mM DTT) and proteins eluted with Strep elution buffer containing 2.5 mM of D-Desthiobiotin (IBA Life Sciences, 2–1000-025). .. The human ARF cDNA was inserted into the Article Title: Mechanistic basis for PYROXD1-mediated protection of the human tRNA ligase complex against oxidative inactivation. Article Snippet: The DNA sequence encoding human Archease, spanning residues 27–168, was inserted into the 2M-T (Addgene, 29708) plasmid using ligation-independent cloning, resulting in a construct carrying an N-terminal hexahistidine tag followed by a maltose binding protein (MBP) and a tobacco etch virus (TEV) protease cleavage site. .. The DNA sequence encoding human RTCB was inserted into the 438B (Addgene, 30115) plasmid and the Article Title: Molecular architecture of the human tRNA ligase complex Article Snippet: DNA sequences encoding human RTCB (UniProt Q9Y3I0) and DDX1 (UniProt Q92499) were cloned into UC Berkeley MacroLab 5B vector (gift from Scott Gradia, Addgene plasmid #30122) with DDX1 inserted into site 1 to generate a fusion with an N-terminal His 6 tag followed by TEV protease cleavage site, and RTCB inserted into site 2 with no affinity tags. .. Similarly, DNA encoding CGI-99 (UniProt Q9Y224) was cloned into site 1 of the UC Berkeley MacroLab 5A vector (gift from Scott Gradia, Addgene plasmid #30121), while DNA sequence encoding FAM98B (UniProt Q52LJ0) N-terminally fused to StrepII tag, GFP, and Article Title: Mechanistic basis for oxidative stress protection of the human tRNA ligase complex by the oxidoreductase PYROXD1 Article Snippet: The human Archease gene spanning residues 27-168 was inserted into the 2M-T (Addgene: 29708) plasmid using ligation-independent cloning (LIC), resulting in a construct carrying an N-terminal hexahistidine tag followed by a maltose binding protein (MBP) and a tobacco etch virus (TEV) protease cleavage site. .. The human RTCB gene was inserted into the 438B (Addgene: 30115) plasmid and the Article Title: ARF alters PAF1 complex integrity to selectively repress oncogenic transcription programs upon p53 loss. Article Snippet: Article Article Title: Mechanistic basis for PYROXD1-mediated protection of the human tRNA ligase complex against oxidative inactivation Article Snippet: The DNA sequence encoding human Archease, spanning residues 27–168, was inserted into the 2M-T (Addgene, 29708) plasmid using ligation-independent cloning, resulting in a construct carrying an N-terminal hexahistidine tag followed by a maltose binding protein (MBP) and a tobacco etch virus (TEV) protease cleavage site. .. The DNA sequence encoding human RTCB was inserted into the 438B (Addgene, 30115) plasmid and the Expressing:Article Title: ARF alters PAF1 complex integrity to selectively repress oncogenic transcription programs upon p53 loss Article Snippet: Beads were washed 4 times with 10 mL Strep wash buffer (20 mM Tris-HCl pH 8.0, 300 mM NaCl, 10% glycerol, 1% NP-40, 1 mM DTT) and proteins eluted with Strep elution buffer containing 2.5 mM of D-Desthiobiotin (IBA Life Sciences, 2–1000-025). .. The human ARF cDNA was inserted into the Article Title: ARF alters PAF1 complex integrity to selectively repress oncogenic transcription programs upon p53 loss. Article Snippet: Article Virus:Article Title: ARF alters PAF1 complex integrity to selectively repress oncogenic transcription programs upon p53 loss Article Snippet: Beads were washed 4 times with 10 mL Strep wash buffer (20 mM Tris-HCl pH 8.0, 300 mM NaCl, 10% glycerol, 1% NP-40, 1 mM DTT) and proteins eluted with Strep elution buffer containing 2.5 mM of D-Desthiobiotin (IBA Life Sciences, 2–1000-025). .. The human ARF cDNA was inserted into the Article Title: ARF alters PAF1 complex integrity to selectively repress oncogenic transcription programs upon p53 loss. Article Snippet: Article other:Article Title: ARF alters PAF1 complex integrity to selectively repress oncogenic transcription programs upon p53 loss Article Snippet: Proteins were eluted with His wash buffer containing 200 mM Imidazole (ThermoFisher, BP305–50). Sequencing:Article Title: Mechanistic basis for PYROXD1-mediated protection of the human tRNA ligase complex against oxidative inactivation. Article Snippet: The DNA sequence encoding human Archease, spanning residues 27–168, was inserted into the 2M-T (Addgene, 29708) plasmid using ligation-independent cloning, resulting in a construct carrying an N-terminal hexahistidine tag followed by a maltose binding protein (MBP) and a tobacco etch virus (TEV) protease cleavage site. .. The DNA sequence encoding human RTCB was inserted into the 438B (Addgene, 30115) plasmid and the Article Title: Molecular architecture of the human tRNA ligase complex Article Snippet: DNA sequences encoding human RTCB (UniProt Q9Y3I0) and DDX1 (UniProt Q92499) were cloned into UC Berkeley MacroLab 5B vector (gift from Scott Gradia, Addgene plasmid #30122) with DDX1 inserted into site 1 to generate a fusion with an N-terminal His 6 tag followed by TEV protease cleavage site, and RTCB inserted into site 2 with no affinity tags. .. Similarly, DNA encoding CGI-99 (UniProt Q9Y224) was cloned into site 1 of the UC Berkeley MacroLab 5A vector (gift from Scott Gradia, Addgene plasmid #30121), while DNA sequence encoding FAM98B (UniProt Q52LJ0) N-terminally fused to StrepII tag, GFP, and Article Title: Mechanistic basis for PYROXD1-mediated protection of the human tRNA ligase complex against oxidative inactivation Article Snippet: The DNA sequence encoding human Archease, spanning residues 27–168, was inserted into the 2M-T (Addgene, 29708) plasmid using ligation-independent cloning, resulting in a construct carrying an N-terminal hexahistidine tag followed by a maltose binding protein (MBP) and a tobacco etch virus (TEV) protease cleavage site. .. The DNA sequence encoding human RTCB was inserted into the 438B (Addgene, 30115) plasmid and the Ligation:Article Title: Mechanistic basis for PYROXD1-mediated protection of the human tRNA ligase complex against oxidative inactivation. Article Snippet: The DNA sequence encoding human Archease, spanning residues 27–168, was inserted into the 2M-T (Addgene, 29708) plasmid using ligation-independent cloning, resulting in a construct carrying an N-terminal hexahistidine tag followed by a maltose binding protein (MBP) and a tobacco etch virus (TEV) protease cleavage site. .. The DNA sequence encoding human RTCB was inserted into the 438B (Addgene, 30115) plasmid and the Article Title: Mechanistic basis for PYROXD1-mediated protection of the human tRNA ligase complex against oxidative inactivation Article Snippet: The DNA sequence encoding human Archease, spanning residues 27–168, was inserted into the 2M-T (Addgene, 29708) plasmid using ligation-independent cloning, resulting in a construct carrying an N-terminal hexahistidine tag followed by a maltose binding protein (MBP) and a tobacco etch virus (TEV) protease cleavage site. .. The DNA sequence encoding human RTCB was inserted into the 438B (Addgene, 30115) plasmid and the Cloning:Article Title: Mechanistic basis for PYROXD1-mediated protection of the human tRNA ligase complex against oxidative inactivation. Article Snippet: The DNA sequence encoding human Archease, spanning residues 27–168, was inserted into the 2M-T (Addgene, 29708) plasmid using ligation-independent cloning, resulting in a construct carrying an N-terminal hexahistidine tag followed by a maltose binding protein (MBP) and a tobacco etch virus (TEV) protease cleavage site. .. The DNA sequence encoding human RTCB was inserted into the 438B (Addgene, 30115) plasmid and the Article Title: Mechanistic basis for PYROXD1-mediated protection of the human tRNA ligase complex against oxidative inactivation Article Snippet: The DNA sequence encoding human Archease, spanning residues 27–168, was inserted into the 2M-T (Addgene, 29708) plasmid using ligation-independent cloning, resulting in a construct carrying an N-terminal hexahistidine tag followed by a maltose binding protein (MBP) and a tobacco etch virus (TEV) protease cleavage site. .. The DNA sequence encoding human RTCB was inserted into the 438B (Addgene, 30115) plasmid and the Clone Assay:Article Title: Molecular architecture of the human tRNA ligase complex Article Snippet: DNA sequences encoding human RTCB (UniProt Q9Y3I0) and DDX1 (UniProt Q92499) were cloned into UC Berkeley MacroLab 5B vector (gift from Scott Gradia, Addgene plasmid #30122) with DDX1 inserted into site 1 to generate a fusion with an N-terminal His 6 tag followed by TEV protease cleavage site, and RTCB inserted into site 2 with no affinity tags. .. Similarly, DNA encoding CGI-99 (UniProt Q9Y224) was cloned into site 1 of the UC Berkeley MacroLab 5A vector (gift from Scott Gradia, Addgene plasmid #30121), while DNA sequence encoding FAM98B (UniProt Q52LJ0) N-terminally fused to StrepII tag, GFP, and Amplification:Article Title: Molecular architecture of the human tRNA ligase complex Article Snippet: DNA sequences encoding human RTCB (UniProt Q9Y3I0) and DDX1 (UniProt Q92499) were cloned into UC Berkeley MacroLab 5B vector (gift from Scott Gradia, Addgene plasmid #30122) with DDX1 inserted into site 1 to generate a fusion with an N-terminal His 6 tag followed by TEV protease cleavage site, and RTCB inserted into site 2 with no affinity tags. .. Similarly, DNA encoding CGI-99 (UniProt Q9Y224) was cloned into site 1 of the UC Berkeley MacroLab 5A vector (gift from Scott Gradia, Addgene plasmid #30121), while DNA sequence encoding FAM98B (UniProt Q52LJ0) N-terminally fused to StrepII tag, GFP, and Produced:Article Title: ARF alters PAF1 complex integrity to selectively repress oncogenic transcription programs upon p53 loss. Article Snippet: Article Infection:Article Title: ARF alters PAF1 complex integrity to selectively repress oncogenic transcription programs upon p53 loss. Article Snippet: Article |