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omniscript reverse transcriptase rt kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher omniscript reverse transcriptase rt kit
    Omniscript Reverse Transcriptase Rt Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reverse+transcriptase+omniscript+rt+kit/pm36797240-278-20-25
    Average 90 stars, based on 1 article reviews
    omniscript reverse transcriptase rt kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Potent LNA oligonucleotides for the inhibition of HIF-1A expression
    Article Snippet: Tissue samples were homogenised using a Retsch 300MM homogeniser and total RNA was isolated using the Trizol reagent or the RNeasy mini kit as described by the manufacturer. .. First Strand Synthesis First strand synthesis was performed using either OmniScript Reverse Transcriptase kit or M-MLV Reverse transcriptase (essentially described by manufacturer (Ambion)) according to the manufacturer's instructions (Qiagen). .. When using OmniScript Reverse Transcriptase 0.5 μg total RNA each sample, was adjusted to 12 μl and mixed with 0.2 μl poly (dT)12-18 (0.5 μg/μl) (Life Technologies), 2 μl dNTP mix (5 mM each), 2 μl 10×RT buffer, 0.5 μl RNAguardTM RNase Inhibitor (33 units/ml, Amersham) and 1 μl OmniScript Reverse Transcriptase followed by incubation at 37° C. for 60 min. and heat inactivation at 93° C. for 5 min.

    Article Title: Polo-like kinase 4 (Plk4) potentiates anoikis-resistance of p53KO mammary epithelial cells by inducing a hybrid EMT phenotype.
    Article Snippet: Cells were harvested and RNA extraction was performed using the RNeasy Kit (Qiagen, USA) according to manufacturer’s instructions. .. Concentration was determined in a ND-1000 spectrometer (Nanodrop) and 1 μg of total RNA was converted to cDNA using the Omniscript Reverse Transcriptase RT Kit (Invitrogen, USA). .. Quantitative Real-Time PCR (qRT-PCR) reaction was performed with TaqMan Gene Expression Assays (Applied Biosystems, USA), using gene-specific PLK4 and P-Cadherin (CDH3) PrimeTime probes (Integrated DNA Technologies, Inc., USA), recognizing specifically the corresponding cDNA sequences, which were amplified for 40 cycles.

    Article Title: Antisense oligomers targeting PCSK9
    Article Snippet: .. First Strand Synthesis First strand synthesis was performed using either OmniScript Reverse Transcriptase kit or M-MLV Reverse transcriptase (essentially as described by manufacturer (Ambion)) according to the manufacturer's instructions (Qiagen). .. When using OmniScript Reverse Transcriptase 0.5 μg total RNA each sample, was adjusted to 12 μl and mixed with 0.2 μl poly (dT)12-18 (0.5 μg/μl) (Life Technologies), 2 μl dNTP mix (5 mM each), 2 μl 10×RT buffer, 0.5 μl RNAguardTM RNase Inhibitor (33 units/mL, Amersham) and 1 μl OmniScript Reverse Transcriptase followed by incubation at 37° C. for 60 min. and heat inactivation at 93° C. for 5 min.

    Article Title: Potent LNA oligonucleotides for the inhibition of HIF-1a expression
    Article Snippet: Tissue samples were homogenised using a Retsch 300MM homogeniser and total RNA was isolated using the Trizol reagent or the RNeasy mini kit as described by the manufacturer. .. First Strand Synthesis First strand synthesis was performed using either OmniScript Reverse Transcriptase kit or M-MLV Reverse transcriptase (essentially described by manufacturer (Ambion)) according to the manufacturer's instructions (Qiagen). .. When using OmniScript Reverse Transcriptase 0.5 μg total RNA each sample, was adjusted to 12 μl and mixed with 0.2 μl poly (dT)12-18 (0.5 μg/μl) (Life Technologies), 2 μl dNTP mix (5 mM each), 2 μl 10× RT buffer, 0.5 μl RNAguardTM RNase Inhibitor (33 units/ml, Amersham) and 1 μl OmniScript Reverse Transcriptase followed by incubation at 37° C. for 60 min. and heat inactivation at 93° C. for 5 min.

    Concentration Assay:

    Article Title: Polo-like kinase 4 (Plk4) potentiates anoikis-resistance of p53KO mammary epithelial cells by inducing a hybrid EMT phenotype.
    Article Snippet: Cells were harvested and RNA extraction was performed using the RNeasy Kit (Qiagen, USA) according to manufacturer’s instructions. .. Concentration was determined in a ND-1000 spectrometer (Nanodrop) and 1 μg of total RNA was converted to cDNA using the Omniscript Reverse Transcriptase RT Kit (Invitrogen, USA). .. Quantitative Real-Time PCR (qRT-PCR) reaction was performed with TaqMan Gene Expression Assays (Applied Biosystems, USA), using gene-specific PLK4 and P-Cadherin (CDH3) PrimeTime probes (Integrated DNA Technologies, Inc., USA), recognizing specifically the corresponding cDNA sequences, which were amplified for 40 cycles.



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