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moloney murine leukemia virus reverse transcriptase enzyme  (BioCifer Pty)

 
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  • 90

    Structured Review

    BioCifer Pty moloney murine leukemia virus reverse transcriptase enzyme
    Moloney Murine Leukemia Virus Reverse Transcriptase Enzyme, supplied by BioCifer Pty, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reverse+transcriptase+enzyme/moloney+murine+leukemia+virus+reverse+transcriptase+enzyme/pmc10694267-76-49-58
    Average 90 stars, based on 1 article reviews
    moloney murine leukemia virus reverse transcriptase enzyme - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Virus:

    Article Title: Development and pre-clinical evaluation of a Zika virus diagnostic for low resource settings
    Article Snippet: ZIKV MR766 culture was mixed into urine and RPMI media samples at a ratio of 1:1 with TNA-Cifer Reagent E (BioCifer, Auchenflower, AUS) at room temperature for 5 min. Urine and RPMI media samples were diluted 1:5 in nuclease free H 2 0. .. Each RT-RAA test was performed using the RAA kit (Jiangsu Qitian Gene Biotechnology Co. Ltd., Wuxi City China) with final reaction conditions of 0.83x RAA rehydration buffer, 1/5 RAA pellet, forward primer (350 nM), reverse primer (350 nM), probe (200 nM), Endonuclease IV (2U; New England Biolabs, Victoria, AUS), Moloney Murine Leukemia virus reverse transcriptase enzyme (M-MLV, 60U; Biocifer Pty Ltd., Auchenflower, AUS), magnesium acetate (MgOAc, 23.33 mM) and 2 μL template in a final reaction volume of 12 μL. ..

    Reverse Transcription:

    Article Title: Development and pre-clinical evaluation of a Zika virus diagnostic for low resource settings
    Article Snippet: ZIKV MR766 culture was mixed into urine and RPMI media samples at a ratio of 1:1 with TNA-Cifer Reagent E (BioCifer, Auchenflower, AUS) at room temperature for 5 min. Urine and RPMI media samples were diluted 1:5 in nuclease free H 2 0. .. Each RT-RAA test was performed using the RAA kit (Jiangsu Qitian Gene Biotechnology Co. Ltd., Wuxi City China) with final reaction conditions of 0.83x RAA rehydration buffer, 1/5 RAA pellet, forward primer (350 nM), reverse primer (350 nM), probe (200 nM), Endonuclease IV (2U; New England Biolabs, Victoria, AUS), Moloney Murine Leukemia virus reverse transcriptase enzyme (M-MLV, 60U; Biocifer Pty Ltd., Auchenflower, AUS), magnesium acetate (MgOAc, 23.33 mM) and 2 μL template in a final reaction volume of 12 μL. ..



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