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rt-pcr kit improm-ii reverse transcription system  (Promega)

 
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    Structured Review

    Promega rt-pcr kit improm-ii reverse transcription system
    Rt Pcr Kit Improm Ii Reverse Transcription System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reverse+transcriptase+enzyme/reverse+transcriptase+enzyme+impromii/pmc05770417-207-18-24
    Average 90 stars, based on 1 article reviews
    rt-pcr kit improm-ii reverse transcription system - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Distal electrical stimulation enhances neuromuscular reinnervation and satellite cell differentiation for functional recovery.
    Article Snippet: .. To prepare first-strand cDNA, mRNA was reversetranscribed with the reverse transcriptase enzyme (ImProm-IITM Reverse Transcriptase, Promega, Madison, WI, USA) in a reaction mixture (20 μL in total). ..

    Article Title: Adipose-derived stem cells modulate neuroinflammation and improve functional recovery in chronic constriction injury of the rat sciatic nerve
    Article Snippet: .. To prepare first-strand cDNA, mRNA was reverse-transcribed with the reverse transcriptase enzyme (ImProm-IITM Reverse Transcriptase, Promega, Madison, WI, USA) in a reaction mixture (20 mL in total). ..

    Article Title: Alterations of the Extracellular Matrix in Colorectal Carcinoma.
    Article Snippet: Background: The process of proliferation and invasion of tumor cells depends on changes in the extracellular matrix (ECM) through the activation of enzymes and alterations in the profile of ECM components.. Our aims were to investigate the mRNA and protein expression profiles of the ECM components, heparanase-1 (HPSE), heparanase-2 (HPSE2), matrix metalloproteinase-9 (MMP-9), and syndecan-1 (SDC1) in neoplastic and nonneoplastic tissues of 24 patients with colorectal carcinoma (CRC) and to test for associations between the expression patterns of these genes with the presence or absence of lymph node metastases.. Materials and Methods: This was a cross-sectional study in which 24 adult patients with CRC were admitted for resectional surgery.

    Article Title: FAM19A5l Affects Mustard Oil-Induced Peripheral Nociception in Zebrafish.
    Article Snippet: To isolate the zebrafish FAM19A5l, total RNA was extracted from zebrafish embryos, larvae, and adult brains using TRIzol reagent (Thermo Fisher Scientific, USA). .. Total RNA was reverse-transcribed into cDNA using a reverse transcription kit (ImProm-IITM Reverse Transcriptase, Promega), according to the manufacturer’s instructions. .. RT-PCR was performed for analyzing FAM19A5l expression using primer sequences designed using Ensembl (FAM19A5l: ENSDARG00000068100).

    Article Title: Tamalin Function Is Required for the Survival of Neurons and Oligodendrocytes in the CNS
    Article Snippet: .. Using the Trizol reagent, we extracted total RNAs from the adult spinal cord and FACS-sorted cells. cDNAs were synthesized from the total RNAs using a reverse transcription kit (ImProm-II TM Reverse Transcriptase, Promega). ..

    Article Title: A γ-Secretase Inhibitor Attenuates Cell Cycle Progression and Invasion in Human Oral Squamous Cell Carcinoma: An In Vitro Study.
    Article Snippet: .. 2022, 23, 8869 11 of 16 cDNA was obtained by converting RNA using a reverse transcriptase kit (ImProm-IITM Reverse Transcription System, Promega, Madison, WI, USA). .. The qRT-PCR reaction was conducted in a CFX connect Real-Time PCR (Bio-Rad, Singapore) with FastStart Essential DNA Green Master (Roche Diagnostic, Mannheim, Germany).

    other:

    Article Title: Assessing the Protective Role of Epigallocatechin Gallate (EGCG) against Water-Pipe Smoke-Induced Toxicity: A Comparative Study on Gene Expression and Histopathology.
    Article Snippet: One microgram of the RNA samples was reversely transcribed into cDNA using reverse transcription with Improm-II Reverse Transcriptase Promega (Madison, WI, USA), following the manufacturer’s protocol and instructions.

    Article Title: Meningeal dendritic cells drive neuropathic pain through elevation of the kynurenine metabolic pathway in mice
    Article Snippet: One microgram of total RNA was transcribed to cDNA by reverse transcriptase enzyme action Improm Pre-II ® (Promega, Madison, Wisconsin, USA).

    FACS:

    Article Title: Tamalin Function Is Required for the Survival of Neurons and Oligodendrocytes in the CNS
    Article Snippet: .. Using the Trizol reagent, we extracted total RNAs from the adult spinal cord and FACS-sorted cells. cDNAs were synthesized from the total RNAs using a reverse transcription kit (ImProm-II TM Reverse Transcriptase, Promega). ..

    Synthesized:

    Article Title: Tamalin Function Is Required for the Survival of Neurons and Oligodendrocytes in the CNS
    Article Snippet: .. Using the Trizol reagent, we extracted total RNAs from the adult spinal cord and FACS-sorted cells. cDNAs were synthesized from the total RNAs using a reverse transcription kit (ImProm-II TM Reverse Transcriptase, Promega). ..



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