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induro rt enzyme  (New England Biolabs)


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    Structured Review

    New England Biolabs induro rt enzyme
    Induro Rt Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 148 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reverse+transcriptase+(rt)+enzymes/Induro+Reverse+Transcriptase/pmc12823552-42-0-3
    Average 98 stars, based on 148 article reviews
    induro rt enzyme - by Bioz Stars, 2026-11
    98/100 stars

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    Reverse Transcription:

    Article Title: Nonsense-mediated mRNA decay factors target short poly(A)-tailed mRNAs lacking a premature termination codon.
    Article Snippet: .. Reverse transcriptions were performed on resulting 5’P and 5’ cap samples using N6 random primer and Induro reverse transcriptase (NEB) according the manufacturer’s instruction. ..

    Article Title: Lactate Dehydrogenase A-Dependent Redox Control of Selective mRNA Translation Directs Monocyte Fate Commitment
    Article Snippet: .. Reverse transcription of the HIF1α 5′ UTR was performed using Induro reverse transcriptase (NEB) with a primer targeting the coding region proximal to the AUG start codon. .. cDNA was amplified for 35 cycles using HIF1α 5′ UTR-specific primers and PrimeStar Max PCR mix (Takara), and PCR product was gel purified.

    Article Title: Inducible, split base editors for in vivo cancer functional genomics.
    Article Snippet: For RNA editing analysis, RNA was extracted from 500,000 cells using an RNeasy Mini kit (74104, Qiagen). .. In total, 0.5–1 μg of RNA was reverse transcribed using Induro Reverse Transcriptase (M0681S, New England Biolabs). .. PCR was performed using the PrimeSTAR GXL Premix system (R051A, Takara).

    Article Title: Systematic assessment of diverse RNA modifications using nanopore direct RNA sequencing
    Article Snippet: .. Reverse transcription was performed by adding a master mix of nuclease-free water, dNTPs (NEB, N0447), and Induro RT Reaction Buffer (NEB, B0681AVIAL), followed by Induro Reverse Transcriptase (NEB, M0681), and incubated at 60°C for 30 min, then 70°C for 10 min. .. The reverse transcription product was purified with Agencourt RNAClean XP beads (Beckman Coulter, A63987) using 70% ethanol washes and eluted in 23 μl nuclease-free water.

    Article Title: Investigation of TRMT61B methyltransferase activity on mRNA and its effects on translation
    Article Snippet: .. Input pooled RNA and purified 80S-bound RNA were then reverse transcribed using Induro RT (NEB, M0681) using oKA210 primer. .. Input pooled RNA and purified 80S-bound RNA were then reverse transcribed using Induro RT (NEB, M0681) using oKA210 primer.

    Article Title: Direct RNA sequencing-based multi-omics profiling reveals transcriptional and post-transcriptional remodeling in mice with diet-induced atherosclerosis
    Article Snippet: Briefly, 9 μL poly(A)+ RNA was combined with 3 μL NEBNext Quick Ligation Reaction Buffer (New England Biolabs, cat. #B6058, United States), 1 μL RT Adapter (Oxford Nanopore Technologies, cat. #SQK-RNA004, United Kingdom), and 2 μL T4 DNA Ligase (New England Biolabs, cat. #M0202, United States), followed by incubation at 25 °C for 10 min to ligate RNA adapters. .. For reverse transcription, 8 μL of 5 × first-strand buffer (New England Biolabs, cat. #M0681, United States), 2 μL of 10 mM Deoxynucleotide (dNTP) Solution Mix (New England Biolabs, cat. #N0447, United States), 9 μL of nuclease-free water, 4 μL of 0.1 M dithiothreitol, and 2 μL Induro Reverse Transcriptase (New England Biolabs, cat. #M0681, United States) were added, followed by incubation at 50 °C for 50 min and at 70 °C for 10 min. .. The reverse-transcribed RNA was purified using 1.8 × Agencourt RNAClean XP beads (Beckman, cat. #A63987, United States).

    Article Title: Aminoacylated tRNA tails trigger auxiliary bacterial immunity
    Article Snippet: .. Reverse transcription was performed using 10 μl pooled RNA library, 8 μl 5× Induro RT Reaction Buffer, 2 μl 10 mM dNTPs, 2 μl Induro reverse transcriptase (New England Biolabs), 0.5 μl RNasin and RNase-free water to a final volume of 40 μl. ..

    Article Title: Cytoplasmic localization of pseudouridine synthase 7 facilitates a pseudouridine-dependent enhancement of cellular stress tolerance.
    Article Snippet: tRNA was prepared by separating total RNA via anion exchange chromatography on a Resource Q column (Cytiva). .. Reverse transcription to generate the IVT-mRNA library was performed with SuperScript III Reverse Transcriptase (Invitrogen 18080044) for RNA002 and Induro® Reverse Transcriptase (NEB M0681) for RNA004. .. Poly-A RNA enrichment of RNA was performed on total RNA using Dynabeads Oligo(dT) 25 (Thermo, 61005) according to the manufacturer’s recommended protocol.

    Incubation:

    Article Title: Systematic assessment of diverse RNA modifications using nanopore direct RNA sequencing
    Article Snippet: .. Reverse transcription was performed by adding a master mix of nuclease-free water, dNTPs (NEB, N0447), and Induro RT Reaction Buffer (NEB, B0681AVIAL), followed by Induro Reverse Transcriptase (NEB, M0681), and incubated at 60°C for 30 min, then 70°C for 10 min. .. The reverse transcription product was purified with Agencourt RNAClean XP beads (Beckman Coulter, A63987) using 70% ethanol washes and eluted in 23 μl nuclease-free water.

    Article Title: Direct RNA sequencing-based multi-omics profiling reveals transcriptional and post-transcriptional remodeling in mice with diet-induced atherosclerosis
    Article Snippet: Briefly, 9 μL poly(A)+ RNA was combined with 3 μL NEBNext Quick Ligation Reaction Buffer (New England Biolabs, cat. #B6058, United States), 1 μL RT Adapter (Oxford Nanopore Technologies, cat. #SQK-RNA004, United Kingdom), and 2 μL T4 DNA Ligase (New England Biolabs, cat. #M0202, United States), followed by incubation at 25 °C for 10 min to ligate RNA adapters. .. For reverse transcription, 8 μL of 5 × first-strand buffer (New England Biolabs, cat. #M0681, United States), 2 μL of 10 mM Deoxynucleotide (dNTP) Solution Mix (New England Biolabs, cat. #N0447, United States), 9 μL of nuclease-free water, 4 μL of 0.1 M dithiothreitol, and 2 μL Induro Reverse Transcriptase (New England Biolabs, cat. #M0681, United States) were added, followed by incubation at 50 °C for 50 min and at 70 °C for 10 min. .. The reverse-transcribed RNA was purified using 1.8 × Agencourt RNAClean XP beads (Beckman, cat. #A63987, United States).

    Purification:

    Article Title: Investigation of TRMT61B methyltransferase activity on mRNA and its effects on translation
    Article Snippet: .. Input pooled RNA and purified 80S-bound RNA were then reverse transcribed using Induro RT (NEB, M0681) using oKA210 primer. .. Input pooled RNA and purified 80S-bound RNA were then reverse transcribed using Induro RT (NEB, M0681) using oKA210 primer.



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