10 × buffer r strep tactin regeneration buffer with haba (IBA Lifesciences)

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IBA Lifesciences 10 × buffer r strep tactin regeneration buffer with haba

10 × Buffer R Strep Tactin Regeneration Buffer With Haba, supplied by IBA Lifesciences, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/regeneration+buffer/Strep-Tactin/pmc11783452-43-0-9
Average 99 stars, based on 1 article reviews

10 × buffer r strep tactin regeneration buffer with haba - by Bioz Stars, 2026-10

99/100 stars

Images

1) Product Images from "MERS-CoV spike vaccine-induced N-terminal domain-specific antibodies are more protective than receptor binding domain-specific antibodies"

Article Title: MERS-CoV spike vaccine-induced N-terminal domain-specific antibodies are more protective than receptor binding domain-specific antibodies

Journal: iScience

doi: 10.1016/j.isci.2024.111632

Figure Legend Snippet:

Techniques Used: Virus, Recombinant, Transfection, Expressing, Plasmid Preparation, Luciferase, Lysis, Suspension, Software, Adjuvant, Electron Microscopy

Isolation:

Article Title: Mutations in the Txnip PPXY Motifs Protect Against Myocardial Infarction Despite Enhanced Protein Stability.
Article Snippet: Immunoblotting was performed using antibodies against TXNIP (clone JY2, MBL 129 International: K0205-3), thioredoxin‐1 (Cell Signaling Technology: 2298), GAPDH (Cell 130 Signaling Technology: 8884 and 2118), LC3 (MBL International: M186-7), α‐tubulin (MBL 131 International: PM054-7), and -actin (Cell Signaling Technology: 4967). .. For pulldown assay, 132 Strep‐tagged proteins were isolated using magnetic Strep‐Tactin beads (IBA, Göttingen, 133 Germany) from cell lysates as previously described (16, 17). .. Pulldown samples were analyzed 134 by Western blotting using anti‐FLAG and anti‐ubiquitin antibodies (Sigma‐Aldrich).

Staining:

Article Title: Interleukin-2 variants with modified biological activity
Article Snippet: .. After wash with PBS/0.2% BSA buffer, cells were stained with an a-Strep-Tag Ab (Strep-Tactin-A488, Iba) for 20 min at 4° C. Cells were acquired on a LSRII flow cytometer and analyzed with FlowJo software. ..

Flow Cytometry:

Software:

Binding Assay:

Article Title: Long-range allosteric communication, double mutant cycles, and energetic coupling in SARS-CoV-2 spike protein
Article Snippet: A concentration of 1 mg/mL of purified spike in 1xPBS, pH 7.4, was measured in triplicate on a NanoTemper Prometheus Panta (NanoTemper Technologies, Munich, Germany). .. First StrepTactin-XT protein (iba-lifesciences, cat# 2-1204-005) was immobilized onto the reference and binding flow channels using manufacturer recommended conditions. ..

Incubation:

Article Title: Mycobacterium tuberculosis long-chain fatty acid resistome reveals universal stress protein TB15.3 as essential for infection.
Article Snippet: .. Protein extracts were incubated with 500 L of 50% Strep-Tactin 4Flow resin (IBA) preequilibrated with Buffer W (100 mM Tris/HCl pH 8.0, 150 mM NaCl, 1 mM EDTA) for 3 h at 4 oC, followed by a wash step with Buffer W. Finally, protein elution was conducted with Buffer E (100 mM Tris/HCl pH 8.0, 150 mM NaCl, AR TI CL E IN P RE SS 1 mM EDTA, 2.5 mM desthiobiotin). ..

Article Title: Nucleoprotein and glycoprotein based serological assays for detection of Marburg virus infections
Article Snippet: .. MaxiSorp 96-well plates (Thermo Fisher Scientific, Cat. no. 442404) were coated with 50 ng/well StrepTactin (IBA Lifesciences, Cat. no. 2-1204) diluted in 100 μL PBS by overnight incubation at +4 °C. .. After three washes with 0.1% Tween-20 in PBS (PBS-T), the plates were blocked with 5% (w/v) nonfat dry milk (Cell Signalling Technology) in the same buffer for 2 h at room temperature.

Filtration:

Article Title: CD164 is an endolysomal host factor for entry of Clade A New World Arenaviruses
Article Snippet: Fc-conjugated proteins were purified by gravity filtration affinity chromatography (BioRad, 7321010), washed with 1 M Tris HCl at pH 8.0, and eluted with 0.2 M Glycine pH 2.2, and neutralized in 1:10 1 M Tris HCl pH 8.8. .. For twin-strep fusion proteins (sGP1s) Strep-Tactin XT 4Flow Resin (IBA Lifesciences, 2-5010-025) was stacked and washed with Buffer W (100 mM Tris-Cl, 150 mM NaCl, 1 mM EDTA pH 8.0) in a gravity filtration affinity chromatography column, filtered supernatants were then poured over the columns and washed in Buffer W and eluted with 50 mM biotin in Buffer W per manufacturers protocol. .. All purified proteins were dialyzed overnight in 1X PBS at 4°C in a dialysis cassette (10,000 MWCO, Slide-A-Lyzer 10K, Thermo Fisher Scientific, 66382), then concentrated with Amicon Ultra centrifugal filters (10,000 MWCO, MilliporeSigma, UFC901008).

Affinity Column:

Purification:

Article Title: Stepwise firing mechanism of an extracellular contractile injection system.
Article Snippet: For the GdCl-treated samples, the residual GdCl was removed through dialysis against TS-buffer using Slide-A-Lyzer MINI dialysis devices (Thermo Fisher). .. The purified AlgoCIS wild-type or AlgoCIS-Alg19StrepII was applied to StrepII-tag affinity purification (Strep-Tactin resins, IBA) and the particles were eluted after several washing steps using TS-buffer containing 5 mM desthiobiotin (Sigma). .. The eluted samples were stained using 1% phosphotungstic acid (PTA, pH 7.0) and imaged on a Morgani TEM (Thermo Fisher).

Affinity Purification:

Blocking Assay:

Article Title: Surface Plasmon Resonance (SPR) Workflow for Comparative Analysis of Nanobody Variants Binding to Lysozyme as a Model Ligand
Article Snippet: 2 g peptone [2% (w/w); Fluka Analytical, cat. no. 87972, or equivalent] 0.5 g yeast extract [0.5% (w/w); Fluka Analytical, cat. no. 92144, or equivalent] 0.06 g NaCl (10 mM; VWR Chemicals, cat. no. 27810.460, or equivalent) 0.02 g KCl (2.5 mM; Sigma‐Aldrich, cat. no. 529552, or equivalent) 0.24 g MgSO 4 (20 mM; Sigma‐Aldrich, cat. no. M7506, or equivalent) Distilled H 2 O to 100 ml Mix well Autoclave Add 2 ml filter‐sterilized 20% (w/w) glucose (Sigma‐Aldrich, cat. no. G7528, or equivalent) Store ≤1 month at 4°C Ensure medium is free of contamination before use; cloudiness or the presence of floating clumps may indicate (microbial) contamination. .. 5 ml Pierce Fast Blocking Buffer (Thermo Fisher Scientific, cat. no. 37575) 1 μl Strep‐Tactin HRP (IBA LifeSciences, cat. no. 2‐1502‐001) Prepare fresh immediately before use and store at 4°C for three more incubations ..

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