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IsoSciences llc 25-hydroxyvitamin d3
25 Hydroxyvitamin D3, supplied by IsoSciences llc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reference+compound/25+hydroxyvitamin+d2+reference+compound/pm30034270-42-3-18
Average 90 stars, based on 1 article reviews
25-hydroxyvitamin d3 - by Bioz Stars, 2026-09
90/100 stars

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Reversed-phase Chromatography:

Article Title: A new method based on dispersive liquid-liquid microextraction for fat-soluble vitamin determination in serum by LC-MS/MS.
Article Snippet: Funding information Hangzhou Biozon Medical Device Co. Ltd; Hangzhou Biozon Medical Laboratory; Key R&D Program of Zhejiang Province, Grant/Award Number: 2021C03088 Abstract A green and inexpensive pretreatment known as dispersive liquid–liquid microextraction (DLLME) was developed in this assay coupled with the LC–MS/MS method for routine analysis of fat soluble vitamins (FSVs).. The technique was performed with methanol as the dispersive solvent and dichloromethane as the extraction solvent.. The extraction phase containing FSVs was evaporated to dryness and reconstituted in a mixture of acetonitrile and water.

Article Title: Vitamin D composition of Australian foods.
Article Snippet: Australia needs accurate vitamin D food composition data to support public health initiatives.. Previously, limitations in analytical methodology have precluded development of a comprehensive database.. We used liquid chromatography with triple quadrupole mass spectrometry (LC-QQQ) to analyse 149 composite samples representing 98 foods (primary samples n = 896) in duplicate for vitamin D3, 25-hydroxyvitamin D3 (25(OH)D3), vitamin D2, 25(OH)D2.

Article Title: Variation in nutritional quality in UK retail eggs.
Article Snippet: Briefly, 1 g of yolk was added to the internal standard i.e. 8 ng 13C5–25-hydroxyvitamin D3 and 8 ng 13C5-vitamin D3 (Isosciences, Ambler, PA), then alkaline saponified and cleaned by liquid-liquid and solid phase extraction (Hybrid SPE (30 mg/1 ml column, Supelco Analytical, Bellefonte, PA, USA).

Article Title: Chronic administration of XBD173 ameliorates cognitive deficits and neuropathology via 18 kDa translocator protein (TSPO) in a mouse model of Alzheimer's disease.
Article Snippet: The following internal standards were added into the extracts for steroid quantification: 2 ng of 13C3-PROG for PROG, 2 ng of 13C5-5α-dihydroprogesterone (DHP) (CDN Isotopes, Sainte Foy la Grande, France) for 5α/β-DHP, 2 ng of epietiocholanolone (3β-hydroxy5β-androstan-17-one, Steraloids, Newport, Rhode Island) for 5α/ β-dihydrotestosterone (DHT), 3α/β5α/β-tetrahydrotestosterone (THT), pregnenolone (PREG), 3α/β5α/β-tetrahydroprogesterone (THPROG), 5α/ β20α-THPROG, 3α/β5α/β20α/β-hexahydroprogesterone (HHPROG) and 3α/ β5α/β-THDOC, (2 ng of 19-norPROG for 5α/β-dihydrodeoxycorticosterone (DHDOC), 10 ng of 2H8-corticosterone (B) for B and 5α/ β-dihydrocorticosterone (DHB), 2 ng of 13C3-deoxycorticosterone (DOC) for DOC and 1 ng of 13C3-testosterone(T) for T. 2 ng of 13C5-20α-DHP (Isosciences) for the analysis of 20α/β-DHP.

Article Title: Aging Is Associated With Lower Neuroactive Steroids and Worsened Outcomes Following Cerebral Ischemia in Male Mice.
Article Snippet: Steroids were extracted from plasma and the ipsilateral hemisphere of brain samples with methanol and the following internal standards were added to the extracts for steroid quantification: 2 ng of 13C5-5α-DHP (Isosciences) for the analysis of 5α/β-DHP and 5α/β-DHADIONE; 1 ng of 13C3-testosterone (Isosciences) for the analysis of testosterone; 1 ng of 13C3-ADIONE (Isosciences) for the analysis of ADIONE; 2 ng of 13C3-progesterone (Isosciences) for the analysis of progesterone; 1 ng of 2H5-17β-estradiol (CDN Isotopes) for the analysis of 17β-estradiol, 2 ng 13C3-DOC (Isosciences) for the analysis of DOC; 2 ng of 19 nor-PROG (Steraloids) for the analysis of 5α/β-DHDOC; 2 ng of epietiocholanolone (Steraloids) for the analysis of 5α/β-DHT, 3α/β5α/β-THT, pregnenolone, 20α-dihydropregnenolone (20α-DHPREG), 3α-dihydroprogesterone (3α-DHP), 3α/β5α/ β-THP, 3α/β5α/β20α/β-hexahydroprogesterone (3α/β5α/ β20α/β-HHP), 3α/β5α/β-THDOC, androstenediol (ADIOL), dehydroepiandrosterone (DHEA), 3α/β5α-tetrahydroandrostenedione (3α/β5α-THA), 10 ng of 2H8-corticosterone (CDN Isotopes) for the analysis of corticosterone, 5α/β-DHB, 3α/ β5α/β-THB, and 11-dehydrocorticosterone, 1 ng of 13C317α-OH-PROG (Isosciences) for the analysis of 17α-hydroxyprogesterone (17α-OH-PROG), 16α-hydroxyprogesterone (16α-OH PROG) and 17α-hydroxypregnenolone (17αOH-PREG); 1 ng of 13C3-estrone (Isosciences) for the analysis of estrone (E1) and 2 ng of 13C5-20α-DHP (Isosciences) for the analysis of 20α/β-dihydroprogesterone (20α/β-DHP).



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