swing rectangular buckets with adapters (Thermo Fisher)
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Swing Rectangular Buckets With Adapters, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rectangular+bucket/swing+rectangular+buckets+with+adapters/pmc07533725-78-57-58
Average 90 stars, based on 1 article reviews
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other:Article Title: RNA interference screening methods to identify proliferation determinants and mechanisms of resistance to immune attack Article Snippet: ER network siRNA library plates (Customized from QIAGEN) siRNA-based library targeting Estrogen receptor (ER)- and aromatase-centered network was designed around 5 seed proteins relevant to estrogen signaling: the ER genes ESR1 (ERα) and ESR2 (ERβ), the estrogen-related receptors ESRRA and ESRRG, and CYP19A1 (aromatase). Negative Control:Article Title: RNA Interference Screening to Identify Proliferation Determinants in Breast Cancer Cells Article Snippet: Materials and Reagents Pipette tips for CyBi-Well Vario 96 channel simultaneous Pipettor (Thermo Fisher Scientific, Thermo Scientific , catalog number: 5587) V-bottom 96-well plates (Corning, catalog number: 3357) Flat-bottom 96-well plates (Corning, catalog number: 3595) 50 ml conical tube Corning 0.22 μm vacuum filter system (Corning, catalog number: 431098) T75 flasks (Corning, Costar) Labels with Barcode MCF7 cells (Tissue Culture Shared Resource, Lombardi Cancer Center, Georgetown Univ.) .. AllStars Negative Control siRNA (QIAGEN, catalog number: 1027281) AllStars Hs Cell Death siRNA (QIAGEN, catalog number: 1027299) AP2A siRNA (QIAGEN, catalog number: SI04371283) GRB14 siRNA (QIAGEN, catalog number: SI00430703) Opti-MEM reduced serum medium (Thermo Fisher Scientific, Gibco , catalog number: 31985070) IMEM medium (Mediatech, catalog number: 10-024-CV) Trypsin-EDTA (0.5%), no phenol red (Thermo Fisher Scientific, Gibco , catalog number: 15400054) Charcoal-stripped bovine calf serum (CCS) (Gemini Bio-Products, catalog number: 100–213) Estradiol (Sigma-Aldrich, catalog number: E8875) Cell Titer Blue (Promega, catalog number: G8082) Hank’s balanced salt solution (HBSS) without calcium, magnesium, phenol red (GE Healthcare, Hyclone , catalog number: SH30588.01) ER network siRNA library plates (Customized from QIAGEN) siRNA suspension buffer (QIAGEN) Lipofectamine RNAiMAX transfection reagent (Thermo Fisher Scientific, Invitrogen , catalog number: 13778500) HiPerfect (QIAGEN, catalog number: 301704) Dharmafect 1–4 transfection reagent (GE Dharmacon, catalog numbers: T-2001, T-2002, T-2003, T-2004) RNAiFect (QIAGEN) 70% (v/v) ethanol (filtered via Corning 0.22 μm vacuum filter system) 0.22 μm filtered ddH 2 O Equipment CyBi-Well Vario 96 channel simultaneous Pipettor (CyBio) Multidrop Combi-nL reagent dispenser (Thermo Fisher Scientific, catalog number: 5840400) WellMate microplate dispenser (Thermo Scientific Matrix) AccuSpin 3R Centrifuge with Ch.003741 rotor (Thermo Fisher Scientific, catalog number: 4393) and Suspension:Article Title: RNA Interference Screening to Identify Proliferation Determinants in Breast Cancer Cells Article Snippet: Materials and Reagents Pipette tips for CyBi-Well Vario 96 channel simultaneous Pipettor (Thermo Fisher Scientific, Thermo Scientific , catalog number: 5587) V-bottom 96-well plates (Corning, catalog number: 3357) Flat-bottom 96-well plates (Corning, catalog number: 3595) 50 ml conical tube Corning 0.22 μm vacuum filter system (Corning, catalog number: 431098) T75 flasks (Corning, Costar) Labels with Barcode MCF7 cells (Tissue Culture Shared Resource, Lombardi Cancer Center, Georgetown Univ.) .. AllStars Negative Control siRNA (QIAGEN, catalog number: 1027281) AllStars Hs Cell Death siRNA (QIAGEN, catalog number: 1027299) AP2A siRNA (QIAGEN, catalog number: SI04371283) GRB14 siRNA (QIAGEN, catalog number: SI00430703) Opti-MEM reduced serum medium (Thermo Fisher Scientific, Gibco , catalog number: 31985070) IMEM medium (Mediatech, catalog number: 10-024-CV) Trypsin-EDTA (0.5%), no phenol red (Thermo Fisher Scientific, Gibco , catalog number: 15400054) Charcoal-stripped bovine calf serum (CCS) (Gemini Bio-Products, catalog number: 100–213) Estradiol (Sigma-Aldrich, catalog number: E8875) Cell Titer Blue (Promega, catalog number: G8082) Hank’s balanced salt solution (HBSS) without calcium, magnesium, phenol red (GE Healthcare, Hyclone , catalog number: SH30588.01) ER network siRNA library plates (Customized from QIAGEN) siRNA suspension buffer (QIAGEN) Lipofectamine RNAiMAX transfection reagent (Thermo Fisher Scientific, Invitrogen , catalog number: 13778500) HiPerfect (QIAGEN, catalog number: 301704) Dharmafect 1–4 transfection reagent (GE Dharmacon, catalog numbers: T-2001, T-2002, T-2003, T-2004) RNAiFect (QIAGEN) 70% (v/v) ethanol (filtered via Corning 0.22 μm vacuum filter system) 0.22 μm filtered ddH 2 O Equipment CyBi-Well Vario 96 channel simultaneous Pipettor (CyBio) Multidrop Combi-nL reagent dispenser (Thermo Fisher Scientific, catalog number: 5840400) WellMate microplate dispenser (Thermo Scientific Matrix) AccuSpin 3R Centrifuge with Ch.003741 rotor (Thermo Fisher Scientific, catalog number: 4393) and Transfection:Article Title: RNA Interference Screening to Identify Proliferation Determinants in Breast Cancer Cells Article Snippet: Materials and Reagents Pipette tips for CyBi-Well Vario 96 channel simultaneous Pipettor (Thermo Fisher Scientific, Thermo Scientific , catalog number: 5587) V-bottom 96-well plates (Corning, catalog number: 3357) Flat-bottom 96-well plates (Corning, catalog number: 3595) 50 ml conical tube Corning 0.22 μm vacuum filter system (Corning, catalog number: 431098) T75 flasks (Corning, Costar) Labels with Barcode MCF7 cells (Tissue Culture Shared Resource, Lombardi Cancer Center, Georgetown Univ.) .. AllStars Negative Control siRNA (QIAGEN, catalog number: 1027281) AllStars Hs Cell Death siRNA (QIAGEN, catalog number: 1027299) AP2A siRNA (QIAGEN, catalog number: SI04371283) GRB14 siRNA (QIAGEN, catalog number: SI00430703) Opti-MEM reduced serum medium (Thermo Fisher Scientific, Gibco , catalog number: 31985070) IMEM medium (Mediatech, catalog number: 10-024-CV) Trypsin-EDTA (0.5%), no phenol red (Thermo Fisher Scientific, Gibco , catalog number: 15400054) Charcoal-stripped bovine calf serum (CCS) (Gemini Bio-Products, catalog number: 100–213) Estradiol (Sigma-Aldrich, catalog number: E8875) Cell Titer Blue (Promega, catalog number: G8082) Hank’s balanced salt solution (HBSS) without calcium, magnesium, phenol red (GE Healthcare, Hyclone , catalog number: SH30588.01) ER network siRNA library plates (Customized from QIAGEN) siRNA suspension buffer (QIAGEN) Lipofectamine RNAiMAX transfection reagent (Thermo Fisher Scientific, Invitrogen , catalog number: 13778500) HiPerfect (QIAGEN, catalog number: 301704) Dharmafect 1–4 transfection reagent (GE Dharmacon, catalog numbers: T-2001, T-2002, T-2003, T-2004) RNAiFect (QIAGEN) 70% (v/v) ethanol (filtered via Corning 0.22 μm vacuum filter system) 0.22 μm filtered ddH 2 O Equipment CyBi-Well Vario 96 channel simultaneous Pipettor (CyBio) Multidrop Combi-nL reagent dispenser (Thermo Fisher Scientific, catalog number: 5840400) WellMate microplate dispenser (Thermo Scientific Matrix) AccuSpin 3R Centrifuge with Ch.003741 rotor (Thermo Fisher Scientific, catalog number: 4393) and Glass:Article Title: RNA Interference Screening to Identify Proliferation Determinants in Breast Cancer Cells Article Snippet: Materials and Reagents Pipette tips for CyBi-Well Vario 96 channel simultaneous Pipettor (Thermo Fisher Scientific, Thermo Scientific , catalog number: 5587) V-bottom 96-well plates (Corning, catalog number: 3357) Flat-bottom 96-well plates (Corning, catalog number: 3595) 50 ml conical tube Corning 0.22 μm vacuum filter system (Corning, catalog number: 431098) T75 flasks (Corning, Costar) Labels with Barcode MCF7 cells (Tissue Culture Shared Resource, Lombardi Cancer Center, Georgetown Univ.) .. AllStars Negative Control siRNA (QIAGEN, catalog number: 1027281) AllStars Hs Cell Death siRNA (QIAGEN, catalog number: 1027299) AP2A siRNA (QIAGEN, catalog number: SI04371283) GRB14 siRNA (QIAGEN, catalog number: SI00430703) Opti-MEM reduced serum medium (Thermo Fisher Scientific, Gibco , catalog number: 31985070) IMEM medium (Mediatech, catalog number: 10-024-CV) Trypsin-EDTA (0.5%), no phenol red (Thermo Fisher Scientific, Gibco , catalog number: 15400054) Charcoal-stripped bovine calf serum (CCS) (Gemini Bio-Products, catalog number: 100–213) Estradiol (Sigma-Aldrich, catalog number: E8875) Cell Titer Blue (Promega, catalog number: G8082) Hank’s balanced salt solution (HBSS) without calcium, magnesium, phenol red (GE Healthcare, Hyclone , catalog number: SH30588.01) ER network siRNA library plates (Customized from QIAGEN) siRNA suspension buffer (QIAGEN) Lipofectamine RNAiMAX transfection reagent (Thermo Fisher Scientific, Invitrogen , catalog number: 13778500) HiPerfect (QIAGEN, catalog number: 301704) Dharmafect 1–4 transfection reagent (GE Dharmacon, catalog numbers: T-2001, T-2002, T-2003, T-2004) RNAiFect (QIAGEN) 70% (v/v) ethanol (filtered via Corning 0.22 μm vacuum filter system) 0.22 μm filtered ddH 2 O Equipment CyBi-Well Vario 96 channel simultaneous Pipettor (CyBio) Multidrop Combi-nL reagent dispenser (Thermo Fisher Scientific, catalog number: 5840400) WellMate microplate dispenser (Thermo Scientific Matrix) AccuSpin 3R Centrifuge with Ch.003741 rotor (Thermo Fisher Scientific, catalog number: 4393) and |

