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Promega reconstitution buffer
Reconstitution Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reconstitution+buffer/reconstitution+buffer/us08592172-148-56-58
Average 90 stars, based on 1 article reviews
reconstitution buffer - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

other:

Article Title: Oxidized glutathione assay
Article Snippet: Luciferin Detection Reagent (LDR) Luciferin Detection Reagent was prepared by reconstituting lyophilized Luciferin Detection Reagent (Promega; Cat. No. V859B) with one bottle of Reconstitution Buffer containing esterase (Promega; Cat. No. V144A).

Article Title: Detection of hydrogen peroxide
Article Snippet: A bottle of Reconstitution Buffer with Esterase (Promega V144B) was thawed and used to reconstitute a Luciferin Detection Reagent (Promega V859B) to create a reconstituted Luciferin Detection Reagent (“LDR”).

Article Title: More than additive effects on liver triglyceride accumulation by combinations of steatotic and non-steatotic pesticides in HepaRG cells
Article Snippet: Detection was performed with the luciferin detection reaction (catalog number: V859A) dissolved in reconstitution buffer with esterase (catalog number: V144A) from Promega (Madison, Wisconsin, USA).

Incubation:

Article Title: Red-shifted luciferins and methods of using same
Article Snippet: .. Reconstitution buffer with esterase (10 ml; Promega Cat. No. V144A) and luciferin detection reagent (Promega; V859A) were mixed and incubated at room temperature for 30 minutes (“Luciferin Detection Reagent”). .. Reconstitution buffer with esterase (10 ml; Promega Cat. No. V144A) and luciferin detection reagent (Promega; V859A) were mixed and incubated at room temperature for 30 minutes (“Luciferin Detection Reagent”).

Article Title: In Vitro Biotransformation of Two Human CYP3A Probe Substrates and Their Inhibition during Early Zebrafish Development
Article Snippet: Subsequently, the microsomal reaction was initiated in each well by the addition of NADPH-regenerating system containing 1.3 mM NADP + , 3.3 mM glucose-6-phosphate, 0.4 U/mL glucose-6-phosphate dehydrogenase and 3.3 mM magnesium chloride (451220 and 451200, Corning Incorporated) in 100 mM KPO 4 buffer (pH 7.4) to the pre-incubation mixture. .. The final reaction mixture was then incubated for 10 min at 37 °C (optimal temperature for HLM) followed by the addition of 50 μL of Luciferin Detection Reagent diluted in Reconstitution Buffer (V859A and V144A, Promega Corporation) to each microplate well to stop the microsomal reaction. ..

Article Title: Red-shifted luciferins and methods of using same
Article Snippet: .. Reconstitution buffer with esterase (10 ml; Promega Cat. No. V144A) and luciferin detection reagent (Promega; V859A) were mixed and incubated at room temperature for 30 minutes (“Luciferin Detection Reagent”). .. Reconstitution buffer with esterase (10 ml; Promega Cat. No. V144A) and luciferin detection reagent (Promega; V859A) were mixed and incubated at room temperature for 30 minutes (“Luciferin Detection Reagent”).

Luciferase:

Article Title: Compounds and methods for detection of superoxide
Article Snippet: .. For luciferin detection, the luciferin-utilizing luciferase enzyme, UltraGlo (Promega Corporation), was prepared by mixing Luciferin Detection Reagent (Promega), Reconstitution Buffer (Promega), and d-Cysteine (Promega), and one volume was added to the reaction. ..



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