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recombinant human rh cripto 1  (R&D Systems)


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    R&D Systems recombinant human rh cripto 1
    Recombinant Human Rh Cripto 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+cripto/Recombinant+Human+Cripto-1+Protein%2C+CF/pm38150919-133-0-4
    Average 93 stars, based on 3 article reviews
    recombinant human rh cripto 1 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Sulforaphane Suppresses the Growth of Triple-negative Breast Cancer Stem-like Cells In vitro and In vivo
    Article Snippet: .. 276 277 ELISA studies 278 279 CR1 (R&D Systems, Cat# 145-CR/CF), Nodal (R&D Systems, Cat# 3218-ND-280 024/CF), GRP78 (Abcam, Cat# ab78432), and Alk4 (Creative BioMart, Cat# 281 ACVR1B-645H,) were purchased from the indicated vendors. ..

    Recombinant:

    Article Title: Effects of a novel Nodal-targeting monoclonal antibody in melanoma
    Article Snippet: SPR analyses were performed on a Biacore 3000 instrument from GE Healthcare, using CM5 sensor chips and certified HBS buffer (20 mM HEPES, 0.15 M NaCl, pH 7.2, P20, 0.005%). .. Recombinant human Nodal ( rh Nodal) and human Cripto-1 ( rh Cripto-1) were from R&D Systems (Minneapolis, Minnesota, USA). .. The following antibodies were used for WB: rabbit anti-Nodal (H-110; Santa Cruz Biotechnology, Dallas TX); rabbit anti-P-Smad2 (44-244G; Life Technologies, Grand Island, NY); rabbit anti-Smad2/3 (07-408; Millipore, Lake Placid, NY); rabbit anti-P-p44/42 MAPK (P-ERK1/2) (9101S; Cell Signaling, Beverley, MA); rabbit anti-p44/24 MAPK (ERK1/2) (44-654-G; Life Technologies); mouse anti-actin (MAB1501; Millipore, Temecula, CA).

    Article Title: Recombinant humanized Fab fragments targeting the CFC domain of human Cripto-1.
    Article Snippet: In the era of immunotherapy, the targeting of disease-specific biomarkers goes hand in hand with the development of highly selective antibody-based reagents having optimal pharmacological/toxicological profiles.. One interesting and debated biomaker for several types of cancers is the onco-fetal protein Cripto-1 that is selectively expressed in many solid tumours and has been actively investigated as potential theranostic target.. Starting from previously described anti-CFC/Cripto-1 murine monoclonal antibodies, we have moved forward to prepare the humanized recombinant Fabs which have been engineered so as to bear an MTGase site useful for a one-step site-specific labelling.

    Concentration Assay:

    Article Title: Recombinant humanized Fab fragments targeting the CFC domain of human Cripto-1.
    Article Snippet: In the era of immunotherapy, the targeting of disease-specific biomarkers goes hand in hand with the development of highly selective antibody-based reagents having optimal pharmacological/toxicological profiles.. One interesting and debated biomaker for several types of cancers is the onco-fetal protein Cripto-1 that is selectively expressed in many solid tumours and has been actively investigated as potential theranostic target.. Starting from previously described anti-CFC/Cripto-1 murine monoclonal antibodies, we have moved forward to prepare the humanized recombinant Fabs which have been engineered so as to bear an MTGase site useful for a one-step site-specific labelling.



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    Selected validation of Nanostring gene expression analysis by qRT-PCR. Four genes including CR1, FOXD3, WNT3, and NOTCH4 are shown here as representatives for amplification. Data are the average of 8 samples from SFN pre-treated group and 8 samples from saline control. All experiments were conducted in duplicates. *: P < 0.05 as compared to the control animals.

    Journal: Cancer prevention research (Philadelphia, Pa.)

    Article Title: Sulforaphane suppresses the growth of triple-negative breast cancer stem-like cells in vitro and in vivo

    doi: 10.1158/1940-6207.CAPR-18-0241

    Figure Lengend Snippet: Selected validation of Nanostring gene expression analysis by qRT-PCR. Four genes including CR1, FOXD3, WNT3, and NOTCH4 are shown here as representatives for amplification. Data are the average of 8 samples from SFN pre-treated group and 8 samples from saline control. All experiments were conducted in duplicates. *: P < 0.05 as compared to the control animals.

    Article Snippet: CR1 (R&D Systems, Cat# 145-CR/CF), Nodal (R&D Systems, Cat# 3218-ND-024/CF), GRP78 (Abcam, Cat# ab78432), and Alk4 (Creative BioMart, Cat# ACVR1B-645H,) were purchased from the indicated vendors.

    Techniques: Biomarker Discovery, Gene Expression, Quantitative RT-PCR, Amplification, Saline, Control

    ELISA assessment of CR1 binding to solid phase BPs including Nodal, GRP78, and Alk4 in the absence (-S) or presence (+S) of 100µM of SFN. CR1 binding to individual BPs in the absence of SFN (-S) condition was normalized to 100%. Percentage reaction induced CR1 binding inhibition was then determined using the 100% reference point for respective BPs. Data represents average values determined from three separate studies with samples run in octuplicate.

    Journal: Cancer prevention research (Philadelphia, Pa.)

    Article Title: Sulforaphane suppresses the growth of triple-negative breast cancer stem-like cells in vitro and in vivo

    doi: 10.1158/1940-6207.CAPR-18-0241

    Figure Lengend Snippet: ELISA assessment of CR1 binding to solid phase BPs including Nodal, GRP78, and Alk4 in the absence (-S) or presence (+S) of 100µM of SFN. CR1 binding to individual BPs in the absence of SFN (-S) condition was normalized to 100%. Percentage reaction induced CR1 binding inhibition was then determined using the 100% reference point for respective BPs. Data represents average values determined from three separate studies with samples run in octuplicate.

    Article Snippet: CR1 (R&D Systems, Cat# 145-CR/CF), Nodal (R&D Systems, Cat# 3218-ND-024/CF), GRP78 (Abcam, Cat# ab78432), and Alk4 (Creative BioMart, Cat# ACVR1B-645H,) were purchased from the indicated vendors.

    Techniques: Enzyme-linked Immunosorbent Assay, Binding Assay, Inhibition