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cripto 1 protein  (R&D Systems)


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    Structured Review

    R&D Systems cripto 1 protein
    Cripto 1 Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+cripto/Recombinant+Human+Cripto-1+Protein/pm29882484-93-0-10
    Average 90 stars, based on 2 article reviews
    cripto 1 protein - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Negative Control:

    Article Title: Cripto-1: an extracellular protein - connecting the sequestered biological dots.
    Article Snippet: Cripto-1 (CR-1) is a multifunctional embryonic protein that is re-expressed during inflammation, wound repair, and malignant transformation.. CR-1 can function either as a tethered co-receptor or shed as a free ligand underpinning its flexible role in cell physiology.. CR-1 has been shown to mediate cell growth, migration, invasion, and induce epithelial to mesenchymal transition (EMT).

    Recombinant:

    Article Title: Cripto-1: an extracellular protein - connecting the sequestered biological dots.
    Article Snippet: Cripto-1 (CR-1) is a multifunctional embryonic protein that is re-expressed during inflammation, wound repair, and malignant transformation.. CR-1 can function either as a tethered co-receptor or shed as a free ligand underpinning its flexible role in cell physiology.. CR-1 has been shown to mediate cell growth, migration, invasion, and induce epithelial to mesenchymal transition (EMT).

    Article Title: Anti-nodal antibodies and methods of using same
    Article Snippet: SPR analyses were performed on a Biacore 3000 instrument from GE Healthcare, using CM5 sensor chips and certified HBS buffer (20 mM HEPES, 0.15 M NaCl, pH 7.2, P20, 0.005%). .. Recombinant human Nodal (rhNodal) and human Cripto-1-1 (rhCripto-1-1) were from R&D Systems (Minneapolis, Minn., USA). .. The following antibodies were used for WB: rabbit anti-Nodal (H-110; Santa Cruz Biotechnology, Dallas Tex.); rabbit anti-P-Smad2 (44-244G; Life Technologies, Grand Island, N.Y.); rabbit anti-Smad2/3 (07-408; Millipore, Lake Placid, N.Y.); rabbit anti-P-p44/42 MAPK (P-ERK1/2) (9101S; Cell Signaling, Beverley, Mass.); rabbit anti-p44/24 MAPK (ERK1/2) (44-654-G; Life Technologies); mouse anti-actin (MAB1501; Millipore, Temecula, Calif.).

    Article Title: Growth Factor Induction of Cripto-1 Shedding by Glycosylphosphatidylinositol-Phospholipase D and Enhancement of Endothelial Cell Migration
    Article Snippet: The plates were blocked with blocking solution (Kirkegaard & Perry Laboratories, Gaithersburg, MD), washed with wash buffer (Kirkegaard & Perry Laboratories), and incubated overnight at 4 °C with 50 l of conditioned media from growth factor-treated cells. .. Human CR-1 recombinant protein (R&D Systems) was used as a standard (supplemental Fig. S1, D and E). ..

    Article Title: Development of conformational antibodies targeting Cripto-1 with neutralizing effects in vitro.
    Article Snippet: Accepted Manuscript Development of conformational antibodies targeting Cripto-1 with neutralizing effects in vitro Giuseppina Focà, Emanuela Iaccarino, Annalia Focà, Luca Sanguigno, Gustavo Untiveros, Maria Cuevas-Nunez, Luigi Strizzi, Antonio Leonardi, Menotti Ruvo, Annamaria Sandomenico PII: S0300-9084(19)30024-0 DOI: https://doi.org/10.1016/j.biochi.2019.01.016 Reference: BIOCHI 5592 To appear in: Biochimie Received Date: 28 August 2018 Accepted Date: 22 January 2019 Please cite this article as: G. Focà, E. Iaccarino, A. Focà, L. Sanguigno, G. Untiveros, M. CuevasNunez, L. Strizzi, A. Leonardi, M. Ruvo, A. Sandomenico, Development of conformational antibodies targeting Cripto-1 with neutralizing effects in vitro, Biochimie, https://doi.org/10.1016/ j.biochi.2019.01.016.. This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.

    Article Title: TGF-β Superfamily Member Nodal Stimulates Human β-Cell Proliferation While Maintaining Cellular Viability
    Article Snippet: The islets were cultured (37°C in 5% CO 2 ) in 24-well, ultralow adherence plates (Corning) at a concentration of 1 IEQ per 1 μL media. .. After 24 hours in serum-free media, recombinant human Nodal (R&D Systems) and recombinant human Cripto (R&D Systems) were added to the media. .. Simultaneously, the nucleotide analog, 5-bromo-2-deoxyuridine (BrdU) was added to a final 10 μM concentration (Sigma Aldrich).

    Incubation:

    Article Title: Cripto-1: an extracellular protein - connecting the sequestered biological dots.
    Article Snippet: Cripto-1 (CR-1) is a multifunctional embryonic protein that is re-expressed during inflammation, wound repair, and malignant transformation.. CR-1 can function either as a tethered co-receptor or shed as a free ligand underpinning its flexible role in cell physiology.. CR-1 has been shown to mediate cell growth, migration, invasion, and induce epithelial to mesenchymal transition (EMT).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Soluble Cripto-1 Induces Accumulation of Supernumerary Centrosomes and Formation of Aberrant Mitoses in Human Embryonic Stem Cells.
    Article Snippet: Page 1 of 25 Stem Cells and Development © Mary Ann Liebert, Inc. DOI: 10.1089/scd.2018.0017 1 St em C el ls an d De ve lo pm en t So lu bl e Cr ip to -1 in du ce s a cc um ul at io n of su pe rn um er ar y ce nt ro so m es a nd fo rm at io n of a be rr an t m ito se s i n hu m an e m br yo ni c s te m ce lls (D OI : 1 0.. 10 89 /s cd .2 01 8.. 00 17 ) Th is pa pe r h as b ee n pe er -re vi ew ed a nd a cc ep te d fo r p ub lic at io n, b ut h as y et to u nd er go co py ed iti ng a nd p ro of co rr ec tio n. T he fi na l p ub lis he d ve rs io n m ay d iff er fr om th is pr oo f. Soluble Cripto-1 induces accumulation of supernumerary centrosomes and formation of aberrant mitoses in human embryonic stem cells Running title: Cripto-1 induces supernumerary centrosomes in hESC



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    Image Search Results


    Selected validation of Nanostring gene expression analysis by qRT-PCR. Four genes including CR1, FOXD3, WNT3, and NOTCH4 are shown here as representatives for amplification. Data are the average of 8 samples from SFN pre-treated group and 8 samples from saline control. All experiments were conducted in duplicates. *: P < 0.05 as compared to the control animals.

    Journal: Cancer prevention research (Philadelphia, Pa.)

    Article Title: Sulforaphane suppresses the growth of triple-negative breast cancer stem-like cells in vitro and in vivo

    doi: 10.1158/1940-6207.CAPR-18-0241

    Figure Lengend Snippet: Selected validation of Nanostring gene expression analysis by qRT-PCR. Four genes including CR1, FOXD3, WNT3, and NOTCH4 are shown here as representatives for amplification. Data are the average of 8 samples from SFN pre-treated group and 8 samples from saline control. All experiments were conducted in duplicates. *: P < 0.05 as compared to the control animals.

    Article Snippet: CR1 (R&D Systems, Cat# 145-CR/CF), Nodal (R&D Systems, Cat# 3218-ND-024/CF), GRP78 (Abcam, Cat# ab78432), and Alk4 (Creative BioMart, Cat# ACVR1B-645H,) were purchased from the indicated vendors.

    Techniques: Biomarker Discovery, Gene Expression, Quantitative RT-PCR, Amplification, Saline, Control

    ELISA assessment of CR1 binding to solid phase BPs including Nodal, GRP78, and Alk4 in the absence (-S) or presence (+S) of 100µM of SFN. CR1 binding to individual BPs in the absence of SFN (-S) condition was normalized to 100%. Percentage reaction induced CR1 binding inhibition was then determined using the 100% reference point for respective BPs. Data represents average values determined from three separate studies with samples run in octuplicate.

    Journal: Cancer prevention research (Philadelphia, Pa.)

    Article Title: Sulforaphane suppresses the growth of triple-negative breast cancer stem-like cells in vitro and in vivo

    doi: 10.1158/1940-6207.CAPR-18-0241

    Figure Lengend Snippet: ELISA assessment of CR1 binding to solid phase BPs including Nodal, GRP78, and Alk4 in the absence (-S) or presence (+S) of 100µM of SFN. CR1 binding to individual BPs in the absence of SFN (-S) condition was normalized to 100%. Percentage reaction induced CR1 binding inhibition was then determined using the 100% reference point for respective BPs. Data represents average values determined from three separate studies with samples run in octuplicate.

    Article Snippet: CR1 (R&D Systems, Cat# 145-CR/CF), Nodal (R&D Systems, Cat# 3218-ND-024/CF), GRP78 (Abcam, Cat# ab78432), and Alk4 (Creative BioMart, Cat# ACVR1B-645H,) were purchased from the indicated vendors.

    Techniques: Enzyme-linked Immunosorbent Assay, Binding Assay, Inhibition