negative control recombinant human cripto 1 r d minneapolis mn  (R&D Systems Hematology)

Journal: Cancer prevention research (Philadelphia, Pa.)

Article Title: Sulforaphane suppresses the growth of triple-negative breast cancer stem-like cells in vitro and in vivo

doi: 10.1158/1940-6207.CAPR-18-0241

Figure Lengend Snippet: Selected validation of Nanostring gene expression analysis by qRT-PCR. Four genes including CR1, FOXD3, WNT3, and NOTCH4 are shown here as representatives for amplification. Data are the average of 8 samples from SFN pre-treated group and 8 samples from saline control. All experiments were conducted in duplicates. *: P < 0.05 as compared to the control animals.

Article Snippet: CR1 (R&D Systems, Cat# 145-CR/CF), Nodal (R&D Systems, Cat# 3218-ND-024/CF), GRP78 (Abcam, Cat# ab78432), and Alk4 (Creative BioMart, Cat# ACVR1B-645H,) were purchased from the indicated vendors.

Techniques: Biomarker Discovery, Gene Expression, Quantitative RT-PCR, Amplification, Saline, Control

Journal: Cancer prevention research (Philadelphia, Pa.)

Article Title: Sulforaphane suppresses the growth of triple-negative breast cancer stem-like cells in vitro and in vivo

doi: 10.1158/1940-6207.CAPR-18-0241

Figure Lengend Snippet: ELISA assessment of CR1 binding to solid phase BPs including Nodal, GRP78, and Alk4 in the absence (-S) or presence (+S) of 100µM of SFN. CR1 binding to individual BPs in the absence of SFN (-S) condition was normalized to 100%. Percentage reaction induced CR1 binding inhibition was then determined using the 100% reference point for respective BPs. Data represents average values determined from three separate studies with samples run in octuplicate.

Article Snippet: CR1 (R&D Systems, Cat# 145-CR/CF), Nodal (R&D Systems, Cat# 3218-ND-024/CF), GRP78 (Abcam, Cat# ab78432), and Alk4 (Creative BioMart, Cat# ACVR1B-645H,) were purchased from the indicated vendors.

Techniques: Enzyme-linked Immunosorbent Assay, Binding Assay, Inhibition