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prime  (Danaher Inc)


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  • 95

    Structured Review

    Danaher Inc prime
    Prime, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 95/100, based on 834 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/readytm+cdna+kit/Ready-To-Go+You-Prime+First-Strand+Beads/pm24998940-153-9-18
    Average 95 stars, based on 834 article reviews
    prime - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Type I Interferon Signaling Controls Gammaherpesvirus Latency In Vivo
    Article Snippet: Total RNA was isolated from tissue samples by homogenization in TRIzol (Life Technologies) using the FastPrep system (MP Biomedicals). .. RNA was treated with DNase I (Promega) and reverse transcribed into cDNA using the Ready-To-Go You-Prime first-strand beads (GE Healthcare) and oligo(dT) 18 primers. .. Genomic DNA was isolated from tissue samples using the QIAamp DNA Mini Kit (Qiagen).

    Article Title: Persistence of Tembusu Virus in Culex tritaeniorhynchus in Yunnan Province, China
    Article Snippet: The total RNA was extracted from virus-infected cells according to the instructions of the QIAamp Viral RNA Mini Kit (Qiagen, Valencia, CA, USA). .. Reverse transcription was performed with Ready-to-Go TM You-Prime First-Strand Beads (GE HealthCare, Little Chalfont, Buckinghamshire, UK) and random primers. ..

    Concentration Assay:

    Article Title: Heterochronic Parabiosis Causes Dacryoadenitis in Young Lacrimal Glands
    Article Snippet: We extracted RNA from excised extraorbital lacrimal glands (male, number of glands: YY = 10, AA = 10, YA-Y = 10, YA-A = 10; female, number of glands: YY = 10, AA = 6, YA-Y = 10, YA-A = 10) according to the manufacturer’s protocol, using a QIAGEN RNeasy Plus Mini RNA isolation kit (Qiagen, Hilden, Germany). .. To generate cDNA, we measured the concentration of RNA, calculated 1 μg of RNA for use, and then used the Ready-To-GoTM You-Prime First-Strand kit (GE Healthcare, Chicago, IL, USA). .. All real-time PCR used minor groove-binding Taqman probes, which were IFN-γ ( Ifng , Mm01168134), major histocompatibility complex class II ( Ciita , Mm00482914), TNF-α ( Tnf , Mm00443258), IL-1β ( Il1b , Mm00434228), Cathepsin S ( Ctss , Mm00457902), CXCL13 ( Cxcl13 , Mm00444533), CXCL9 ( Cxcl9 , Mm00434946), and CD19 ( Cd19 , Mm00515420).

    Article Title: Comparison of Efficacy and Inflammatory Response to Thermoconjunctivoplasty Performed with Cautery or Pulsed 1460 nm Laser
    Article Snippet: Conjunctival Gene Expression: Following euthanasia, conjunctiva was excised on days 3 and 10 and total RNA was extracted using a QIAGEN RNeasy Plus Micro RNA isolation kit (Qiagen) following the manufacturer’s protocol. .. RNA concentration was measured, and cDNA was synthesized using the Ready-To-GoTM You-Prime First-Strand kit (GE Healthcare). .. Quantitative real-time PCR was performed with specific murine MGB probes: IL-1b (Mm00434228), TNF-a (Mm00443260), Chemokine (C-C motif) ligand 2 (Mm00441242), Transforming Growth Factor Beta-1 (TGFβ1, Mm00441724), TGF-β2 (Mm00436952_m1), VEGF, and hypoxanthine phosphoribosyltransferase (HPRT1) (Mm00446968).

    other:

    Article Title: Imbalanced IL-37/TNF-α/CTSS signaling disrupts corneal epithelial barrier in a dry eye model in vitro
    Article Snippet: The 2D and 3D digital images were captured with a laser scanning confocal microscope (Nikon A1 RMP, Nikon, Melville, NY) wavelength 400–750 nm and one μm z-step.

    Synthesized:

    Article Title: Comparison of Efficacy and Inflammatory Response to Thermoconjunctivoplasty Performed with Cautery or Pulsed 1460 nm Laser
    Article Snippet: Conjunctival Gene Expression: Following euthanasia, conjunctiva was excised on days 3 and 10 and total RNA was extracted using a QIAGEN RNeasy Plus Micro RNA isolation kit (Qiagen) following the manufacturer’s protocol. .. RNA concentration was measured, and cDNA was synthesized using the Ready-To-GoTM You-Prime First-Strand kit (GE Healthcare). .. Quantitative real-time PCR was performed with specific murine MGB probes: IL-1b (Mm00434228), TNF-a (Mm00443260), Chemokine (C-C motif) ligand 2 (Mm00441242), Transforming Growth Factor Beta-1 (TGFβ1, Mm00441724), TGF-β2 (Mm00436952_m1), VEGF, and hypoxanthine phosphoribosyltransferase (HPRT1) (Mm00446968).



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