tbars assay kit (Elabscience Biotechnology)

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Elabscience Biotechnology tbars assay kit

Effects of PAQR3 overexpression on ferroptosis in HCC cells. (a) <t>Lipid</t> <t>peroxidation</t> levels measured by <t>TBARS</t> assay. (b, c) ROS levels detected by C11-BODIPY staining (red/green ratio; scale bars: 50 μm). (d, e) Intracellular Fe 2+ levels assessed by fluorescence intensity (scale bars: 50 μm). (f) Representative western blot bands of ferroptosis-related proteins (SLC7A11, GPX4, ACSL4, and b-actin). (g) Quantification of relative SLC7A11 expression. (h) Quantification of relative GPX4 expression. (i) Quantification of relative ACSL4 expression. ✶ ✶ ✶ P < 0.001, ns: Not significant. Data in (a, g, h, i) are presented as mean±standard deviation from three independent experiments. Data in (b-e) and blot in (f) are representative of three independent experiments. PAQR3: Progestin and adipoQ receptor 3, HCC: Hepatocellular carcinoma, TBARS: Thiobarbituric acid reactive substance, ROS: Reactive oxygen species, Oe-NC: Overexpression negative control.

Tbars Assay Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reactants/Thiobarbituric+Acid+Reactants+(TBARS)+Colorimetric+Assay+Kit/pmc12883101-69-13-16
Average 94 stars, based on 38 article reviews

tbars assay kit - by Bioz Stars, 2026-09

94/100 stars

Images

1) Product Images from "A novel candidate tumor biomarker progestin and adipoQ receptor 3 regulates cell metastasis through transforming growth factor-β pathway in hepatocellular carcinoma"

Article Title: A novel candidate tumor biomarker progestin and adipoQ receptor 3 regulates cell metastasis through transforming growth factor-β pathway in hepatocellular carcinoma

Journal: CytoJournal

doi: 10.25259/Cytojournal_138_2025

Effects of PAQR3 overexpression on ferroptosis in HCC cells. (a) Lipid peroxidation levels measured by TBARS assay. (b, c) ROS levels detected by C11-BODIPY staining (red/green ratio; scale bars: 50 μm). (d, e) Intracellular Fe 2+ levels assessed by fluorescence intensity (scale bars: 50 μm). (f) Representative western blot bands of ferroptosis-related proteins (SLC7A11, GPX4, ACSL4, and b-actin). (g) Quantification of relative SLC7A11 expression. (h) Quantification of relative GPX4 expression. (i) Quantification of relative ACSL4 expression. ✶ ✶ ✶ P < 0.001, ns: Not significant. Data in (a, g, h, i) are presented as mean±standard deviation from three independent experiments. Data in (b-e) and blot in (f) are representative of three independent experiments. PAQR3: Progestin and adipoQ receptor 3, HCC: Hepatocellular carcinoma, TBARS: Thiobarbituric acid reactive substance, ROS: Reactive oxygen species, Oe-NC: Overexpression negative control.

Figure Legend Snippet: Effects of PAQR3 overexpression on ferroptosis in HCC cells. (a) Lipid peroxidation levels measured by TBARS assay. (b, c) ROS levels detected by C11-BODIPY staining (red/green ratio; scale bars: 50 μm). (d, e) Intracellular Fe 2+ levels assessed by fluorescence intensity (scale bars: 50 μm). (f) Representative western blot bands of ferroptosis-related proteins (SLC7A11, GPX4, ACSL4, and b-actin). (g) Quantification of relative SLC7A11 expression. (h) Quantification of relative GPX4 expression. (i) Quantification of relative ACSL4 expression. ✶ ✶ ✶ P < 0.001, ns: Not significant. Data in (a, g, h, i) are presented as mean±standard deviation from three independent experiments. Data in (b-e) and blot in (f) are representative of three independent experiments. PAQR3: Progestin and adipoQ receptor 3, HCC: Hepatocellular carcinoma, TBARS: Thiobarbituric acid reactive substance, ROS: Reactive oxygen species, Oe-NC: Overexpression negative control.

Techniques Used: Over Expression, TBARS Assay, Staining, Fluorescence, Western Blot, Expressing, Standard Deviation, Negative Control

PAQR3 regulates HCC cell metastasis and ferroptosis through the TGF-b pathway. (a) Wound healing assay images showing cell migration in different groups (scale bars: 200 μm). (b) Quantification of cell migration rates from wound healing assay. (c) Transwell invasion assay images of HCC cells in different groups (scale bars: 50 μm). (d) Quantification of invaded cell counts from the Transwell assay. (e) Representative Western blot images of EMT-related proteins (E-cadherin, N-cadherin, Vimentin, b-actin). (f) Quantification of relative E-cadherin expression. (g) Quantification of relative N-cadherin expression. (h) Quantification of relative Vimentin expression. (i) TBARS assay showing lipid peroxidation levels. (j) C11-BODIPY staining showing ROS levels (merge, oxidized C11, non-oxidized C11, scale bars: 50 μm). (k) Representative images of intracellular Fe 2+ fluorescence (scale bars: 50 μm). (l) Quantification of red/green fluorescence ratio for ROS levels. (m) Quantification of intracellular Fe 2+ fluorescence intensity. (n) Representative western blot images of ferroptosis-related proteins (SLC7A11, GPX4, ACSL4, b-actin). (o) Quantification of relative SLC7A11 expression. (p) Quantification of relative GPX4 expression. (q) Quantification of relative ACSL4 expression. ✶ P < 0.05, ✶ ✶ P < 0.01, ✶ ✶ ✶ P < 0.001, ns: Not significant. Images in (a, c, e, j, k, n) are representative of three independent experiments. Quantitative data in (b, d, f-h, i, l, m, o-q) are presented as mean±SD from three independent experiments. PAQR3: Progestin and adipoQ receptor 3, HCC: Hepatocellular carcinoma, EMT: Epithelial-mesenchymal transition, TBARS: Thiobarbituric acid reactive substance, Oe-NC: Overexpression negative control.

Figure Legend Snippet: PAQR3 regulates HCC cell metastasis and ferroptosis through the TGF-b pathway. (a) Wound healing assay images showing cell migration in different groups (scale bars: 200 μm). (b) Quantification of cell migration rates from wound healing assay. (c) Transwell invasion assay images of HCC cells in different groups (scale bars: 50 μm). (d) Quantification of invaded cell counts from the Transwell assay. (e) Representative Western blot images of EMT-related proteins (E-cadherin, N-cadherin, Vimentin, b-actin). (f) Quantification of relative E-cadherin expression. (g) Quantification of relative N-cadherin expression. (h) Quantification of relative Vimentin expression. (i) TBARS assay showing lipid peroxidation levels. (j) C11-BODIPY staining showing ROS levels (merge, oxidized C11, non-oxidized C11, scale bars: 50 μm). (k) Representative images of intracellular Fe 2+ fluorescence (scale bars: 50 μm). (l) Quantification of red/green fluorescence ratio for ROS levels. (m) Quantification of intracellular Fe 2+ fluorescence intensity. (n) Representative western blot images of ferroptosis-related proteins (SLC7A11, GPX4, ACSL4, b-actin). (o) Quantification of relative SLC7A11 expression. (p) Quantification of relative GPX4 expression. (q) Quantification of relative ACSL4 expression. ✶ P < 0.05, ✶ ✶ P < 0.01, ✶ ✶ ✶ P < 0.001, ns: Not significant. Images in (a, c, e, j, k, n) are representative of three independent experiments. Quantitative data in (b, d, f-h, i, l, m, o-q) are presented as mean±SD from three independent experiments. PAQR3: Progestin and adipoQ receptor 3, HCC: Hepatocellular carcinoma, EMT: Epithelial-mesenchymal transition, TBARS: Thiobarbituric acid reactive substance, Oe-NC: Overexpression negative control.

Techniques Used: Wound Healing Assay, Migration, Transwell Invasion Assay, Transwell Assay, Western Blot, Expressing, TBARS Assay, Staining, Fluorescence, Over Expression, Negative Control

Clinical Proteomics:

Article Title: Perfusate Liver Arginase 1 Levels After End-Ischemic Machine Perfusion Are Associated with Early Allograft Dysfunction.
Article Snippet: .. TBARS levels were measured in plasma and perfusate samples using a colorimetric method by measuring the OD values at 530–540 nm, according to the manufacturer’s specifications (E-BC-K298-M, Elabscience Biotechnology Co., Ltd., Wuhan, China). ..

Article Title: Perfusate Liver Arginase 1 Levels After End-Ischemic Machine Perfusion Are Associated with Early Allograft Dysfunction
Article Snippet: .. TBARS levels were measured in plasma and perfusate samples using a colorimetric method by measuring the OD values at 530–540 nm, according to the manufacturer’s specifications (E-BC-K298-M, Elabscience Biotechnology Co., Ltd., Wuhan, China). ..

Colorimetric Assay:

Article Title: Antibacterial mechanisms of diacetyl on Listeria monocytogenes and its application in Inner Mongolian cheese preservation via gelatin-based edible films
Article Snippet: Cheese spoilage caused by Listeria monocytogenes (L. monocytogenes) contamination results in food waste, economic losses, and even threatens human health.. Diacetyl, a natural microbial metabolite derived from lactic acid bacteria with efficient antimicrobial activity, is commonly found in fermented dairy products, but its inhibitory effect on L. monocytogenes was few reported.. This study aimed to explore the antibacterial effects and mechanisms of diacetyl on L. monocytogenes, and its application in Inner Mongolian cheese preservation was conducted by fabricating it into gelatin-based edible films.

Article Title: Knockdown of EFEMP1 Promotes Ferroptosis by Inactivating PI3K/AKT to overcome the Resistance of Hepatocellular Carcinoma Cells to Sorafenib.
Article Snippet: .. Thiobarbituric Acid Reactive Substances Assay Thiobarbituric Acid Reactive Substances (TBARS) level in HCCLM3, HCCLM3-SR, Huh7 and Huh7-SR cells was detected with a TBARS Colorimetric Assay kit (E-BC-K298-M, Elabscience, Wuhan, China). ..

Article Title: Exploring the anti-Listeria monocytogenes mechanisms of the postbiotics derived from Ligilactobacillus salivarius LSA-6 and its biopreservative effects on Inner Mongolian cheese
Article Snippet: Food contamination caused by Listeria monocytogenes has been a major concern worldwide, and postbiotics derived from lactic acid bacteria are promising in controlling foodborne pathogens, while the antibacterial effects and mechanisms are still largely unclear.. This study aimed to investigate the anti-L. monocytogenes activity and mechanisms of the postbiotics derived from Ligilactobacillus salivarius LSA-6 (Postbio-LSA-6), and its potential application in Inner Mongolian cheese preservation.. Results showed Postbio-LSA-6 exhibited efficient anti-L. monocytogenes activity, the minimal inhibitory concentration (MIC) was 4 mg/mL.

TBARS Assay:

Article Title: A novel candidate tumor biomarker progestin and adipoQ receptor 3 regulates cell metastasis through transforming growth factor-β pathway in hepatocellular carcinoma
Article Snippet: Cells adhering to the underside of the membrane were then stained with crystal violet solution for 15 min (Beyotime Institute of Biotechnology, Y268091, Haimen, Jiangsu, China), and images were randomly captured using an inverted microscope (Leica Microsystem, Mateo FL, Wetzlar, Germany). .. Lipid peroxidation in HCC cells following the indicated treatments was assessed using a TBARS Assay Kit (Elabscience Biotechnology Co., Ltd., E-BC-K298-M, Wuhan, Hubei, China). ..

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