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thiobarbituric acid reactive substances tbars assay kit  (Elabscience Biotechnology)


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    Structured Review

    Elabscience Biotechnology thiobarbituric acid reactive substances tbars assay kit
    Effect of OI on the infiltration of innate immune cells and oxidative stress in mice. (A) Immunofluorescence detection of F4/80-positive cells in hepatic sections from each group; DAPI was used to show nucleus. (B) Immunohistochemical detection of Ly6G-positive cells in liver sections of each group. (C) Positive F4/80 cells per field were analyzed for five visual fields per liver sections from each group. (D) Positive Ly6G cells per field were analyzed in five visual fields per liver section from each group. (E) The activities of SOD were measured in each group. (F) <t>TBARS</t> levels were measured in each group. (G) Activities of MPO were evaluated in each group. (H) The GSH/GSSG ratio was measured in each group. Original magnification, ×200. Data are expressed as mean ± SEM ( n = 5 for each group); # p < 0.05 vs. control group; * p < 0.05 vs. CCl4-treated group.
    Thiobarbituric Acid Reactive Substances Tbars Assay Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reactants/Thiobarbituric+Acid+Reactants+(TBARS)+Fluorometric+Assay+Kit/pmc08185275-48-0-10
    Average 94 stars, based on 8 article reviews
    thiobarbituric acid reactive substances tbars assay kit - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "4-OI Attenuates Carbon Tetrachloride-Induced Hepatic Injury via Regulating Oxidative Stress and the Inflammatory Response"

    Article Title: 4-OI Attenuates Carbon Tetrachloride-Induced Hepatic Injury via Regulating Oxidative Stress and the Inflammatory Response

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2021.651444

    Effect of OI on the infiltration of innate immune cells and oxidative stress in mice. (A) Immunofluorescence detection of F4/80-positive cells in hepatic sections from each group; DAPI was used to show nucleus. (B) Immunohistochemical detection of Ly6G-positive cells in liver sections of each group. (C) Positive F4/80 cells per field were analyzed for five visual fields per liver sections from each group. (D) Positive Ly6G cells per field were analyzed in five visual fields per liver section from each group. (E) The activities of SOD were measured in each group. (F) TBARS levels were measured in each group. (G) Activities of MPO were evaluated in each group. (H) The GSH/GSSG ratio was measured in each group. Original magnification, ×200. Data are expressed as mean ± SEM ( n = 5 for each group); # p < 0.05 vs. control group; * p < 0.05 vs. CCl4-treated group.
    Figure Legend Snippet: Effect of OI on the infiltration of innate immune cells and oxidative stress in mice. (A) Immunofluorescence detection of F4/80-positive cells in hepatic sections from each group; DAPI was used to show nucleus. (B) Immunohistochemical detection of Ly6G-positive cells in liver sections of each group. (C) Positive F4/80 cells per field were analyzed for five visual fields per liver sections from each group. (D) Positive Ly6G cells per field were analyzed in five visual fields per liver section from each group. (E) The activities of SOD were measured in each group. (F) TBARS levels were measured in each group. (G) Activities of MPO were evaluated in each group. (H) The GSH/GSSG ratio was measured in each group. Original magnification, ×200. Data are expressed as mean ± SEM ( n = 5 for each group); # p < 0.05 vs. control group; * p < 0.05 vs. CCl4-treated group.

    Techniques Used: Immunofluorescence, Immunohistochemical staining, Control

    ML385 diminished the protective effects of OI in CCl4-induced liver injury in mice. Representative H&E staining images for the necrotic area (A) and TUNEL staining hepatic sections for necrotic cells (B) from the CCl4, OI + CCl4 OI + CCl4 + ML385 group. ML385 blocked the protective effect of OI. Next, the statistical analysis of necrotic area (C) and quantification of TUNEL-positive cells (D) were performed. Serum activities of ALT (E) and AST (F) were analyzed in CCl4, OI + CCl4, and OI + CCl4 + ML385 groups. SOD (G) activity and the level of TBARS (H) were analyzed in the CCl4, OI + CCl4, and OI + CCl4 + ML385 groups. Original magnification, ×200. Data were expressed as mean ± SEM ( n = 5 for each group); # p < 0.05 vs. CCl4 group; * p < 0.05 vs. CCl4 + OI group.
    Figure Legend Snippet: ML385 diminished the protective effects of OI in CCl4-induced liver injury in mice. Representative H&E staining images for the necrotic area (A) and TUNEL staining hepatic sections for necrotic cells (B) from the CCl4, OI + CCl4 OI + CCl4 + ML385 group. ML385 blocked the protective effect of OI. Next, the statistical analysis of necrotic area (C) and quantification of TUNEL-positive cells (D) were performed. Serum activities of ALT (E) and AST (F) were analyzed in CCl4, OI + CCl4, and OI + CCl4 + ML385 groups. SOD (G) activity and the level of TBARS (H) were analyzed in the CCl4, OI + CCl4, and OI + CCl4 + ML385 groups. Original magnification, ×200. Data were expressed as mean ± SEM ( n = 5 for each group); # p < 0.05 vs. CCl4 group; * p < 0.05 vs. CCl4 + OI group.

    Techniques Used: Staining, TUNEL Assay, Activity Assay

    Related Articles

    TBARS Assay:

    Article Title: 4-OI Attenuates Carbon Tetrachloride-Induced Hepatic Injury via Regulating Oxidative Stress and the Inflammatory Response.
    Article Snippet: Alanine transaminase (ALT), aspartate transaminase (AST), myeloperoxidase (MPO), reduced glutathione/oxidized glutathione assay kit (GSH/GSSG) and superoxide dismutase (SOD) assay kit were purchased from Nanjing Jiancheng Institute of Biotechnology (Nanjing, China). .. Thiobarbituric acid reactive substances (TBARS) assay kit was obtained from Elabscience (Wuhan, China). .. 2,7- Dichlorofluorescein diacetate (DCFH-DA), 4’6-diamidino-2phenylindole (DAPI) and BeyoECL Plus were bought from Beyotime Biotechnology (Shanghai, China).

    Article Title: RBC-derived nanosystem with enhanced ferroptosis triggered by oxygen-boosted phototherapy for synergized tumor treatment.
    Article Snippet: Photodynamic and ferroptosis therapies for cancer treatment are restricted by the scarcity of oxygen and Fe in cancer cells, and the complicated structure of delivery systems.. Herein, a red blood cell-derived vehicle (RDV) inherently enriched with hemoglobin co-delivers a photosensitizer, Ce6, and a ferroptosis promoter, sorafenib (SRF) into cancer cells for boosting oxygen and providing iron, which leads to enhanced PDT and stronger ferroptosis therapy.. Damage to the RDV membrane under local irradation leads to SRF release at the tumor site for tumor-targeted therapy.

    Article Title: Study on the mechanism of traditional chinese medicine compound Shixiang Plaster improving diabetic wound healing through MAPK regulation of macrophage polarization.
    Article Snippet: The healing impairment of diabetic foot ulcers (DFU) is closely related to chronic inflammation and the imbalance of M1/M2 polarization of macrophages.. This study aims to elucidate the mechanism by which the traditional Chinese medicine compound Shixiang Plaster (SXP) promotes wound healing in diabetes by regulating the MAPK signaling pathway and modulating macrophage polarization.. A full-thickness skin defect model in streptozotocin (STZ)-induced diabetic mice was established and divided into the control group, model group, SXP group, and basic fibroblast growth factor (BFGF) group.

    Article Title: 4-OI Attenuates Carbon Tetrachloride-Induced Hepatic Injury via Regulating Oxidative Stress and the Inflammatory Response
    Article Snippet: Alanine transaminase (ALT), aspartate transaminase (AST), myeloperoxidase (MPO), reduced glutathione/oxidized glutathione assay kit (GSH/GSSG) and superoxide dismutase (SOD) assay kit were purchased from Nanjing Jiancheng Institute of Biotechnology (Nanjing, China). .. Thiobarbituric acid reactive substances (TBARS) assay kit was obtained from Elabscience (Wuhan, China). .. 2,7-Dichlorofluorescein diacetate (DCFH-DA), 4’6-diamidino-2-phenylindole (DAPI) and BeyoECL Plus were bought from Beyotime Biotechnology (Shanghai, China).

    Multiple Displacement Amplification:

    Article Title: Study on the mechanism of traditional chinese medicine compound Shixiang Plaster improving diabetic wound healing through MAPK regulation of macrophage polarization.
    Article Snippet: The healing impairment of diabetic foot ulcers (DFU) is closely related to chronic inflammation and the imbalance of M1/M2 polarization of macrophages.. This study aims to elucidate the mechanism by which the traditional Chinese medicine compound Shixiang Plaster (SXP) promotes wound healing in diabetes by regulating the MAPK signaling pathway and modulating macrophage polarization.. A full-thickness skin defect model in streptozotocin (STZ)-induced diabetic mice was established and divided into the control group, model group, SXP group, and basic fibroblast growth factor (BFGF) group.

    Marker:

    Article Title: Study on the mechanism of traditional chinese medicine compound Shixiang Plaster improving diabetic wound healing through MAPK regulation of macrophage polarization.
    Article Snippet: The healing impairment of diabetic foot ulcers (DFU) is closely related to chronic inflammation and the imbalance of M1/M2 polarization of macrophages.. This study aims to elucidate the mechanism by which the traditional Chinese medicine compound Shixiang Plaster (SXP) promotes wound healing in diabetes by regulating the MAPK signaling pathway and modulating macrophage polarization.. A full-thickness skin defect model in streptozotocin (STZ)-induced diabetic mice was established and divided into the control group, model group, SXP group, and basic fibroblast growth factor (BFGF) group.

    Fluorescence:

    Article Title: Study on the mechanism of traditional chinese medicine compound Shixiang Plaster improving diabetic wound healing through MAPK regulation of macrophage polarization.
    Article Snippet: The healing impairment of diabetic foot ulcers (DFU) is closely related to chronic inflammation and the imbalance of M1/M2 polarization of macrophages.. This study aims to elucidate the mechanism by which the traditional Chinese medicine compound Shixiang Plaster (SXP) promotes wound healing in diabetes by regulating the MAPK signaling pathway and modulating macrophage polarization.. A full-thickness skin defect model in streptozotocin (STZ)-induced diabetic mice was established and divided into the control group, model group, SXP group, and basic fibroblast growth factor (BFGF) group.



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