random primer (TaKaRa)

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TaKaRa random primer
Random Primer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 6525 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/random/Random+Primer/pmc13652265-197-0-9
Average 96 stars, based on 6525 article reviews

random primer - by Bioz Stars, 2026-10

96/100 stars

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Synthesized:

Article Title: Retinoic Acid-inducible Gene-I Increases Interferon-stimulated Gene 15 Expression in Human Renal Proximal Tubule Epithelial Cells
Article Snippet: .. Single-stranded complementary DNA was synthesized from purified total RNA using random primers (TAKARA BIO, Kusatsu, Japan), dNTP mix (Thermo Fisher Scientific), and M-MLV reverse transcriptase (Thermo Fisher Scientific). .. RT-qPCR for RIG-I, ISG15, UBA7, USP18, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was performed on the CFX real-time PCR detection system (Bio-Rad, Hercules, CA, USA) using specific primers and SsoAdvanced Universal SYBR Green Supermix (Bio-Rad).

Purification:

Reverse Transcription:

Article Title: A novel diagnostic model for HIV–HTN comorbidity: genomic discovery, clinical validation, and mechanistic elucidation
Article Snippet: Total RNA was isolated from whole blood using TRIzol reagent (Invitrogen) following the manufacturer’s guidelines. .. The obtained RNA was reverse-transcribed into cDNA with random primers and a commercial kit (Takara, China). .. Reverse transcription was conducted at 37°C for 15 min and terminated at 85°C for 5 s. Quantitative real-time PCR (qRT-PCR) was subsequently performed with the Power SYBR Green kit (Takara, China) on an ABI 7,500 platform.

Article Title: Cucumber Mosaic Virus Coat Protein Sequesters Host CDPK7 ‐Like Into Phase‐Separated Condensates to Promote Viral Infection
Article Snippet: The leaves of the assayed N. benthamiana were used to extract total RNA by RNAiso Plus (TaKaRa). .. Reverse transcription was carried out using gene‐specific primers or random primers and a reverse transcriptase kit (TaKaRa) according to the instructions. .. The 2× Hieff PCR Master Mix (Yeasen) was used to execute PCR. qPCR was conducted using the TB Green Premix Ex Taq II (TaKaRa).

cDNA Synthesis:

Article Title: Telomere maintenance mechanisms are activated in ganglioneuroblastoma and ganglioneuroma
Article Snippet: .. Total RNA from human frozen tissue was extracted by ISOGEN II (NIPPON GENE) and cDNA synthesis was performed by ReverTra Ace ® (TOYOBO) and random primers (Takara Bio) according to the manufacturer's instructions. .. RT-qPCR analysis was conducted in triplicate using QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems).

Article Title: CircPRKCA Promotes NSCLC Progression via miR-200b-3p/FRMD6/SNAI2 Axis.
Article Snippet: .. To validate circRNA backspliced junctions, total RNA was treated with the RiboZero rRNA Removal Kit (Epicentre, Madison, WI, USA) to deplete rRNA (per the manufacturer’s instructions), followed by cDNA synthesis from rRNA-depleted and RNase R-digested RNA using random primers (Takara, Dalian, China). .. For mRNA and circRNA quantification, cDNA was synthesized from 500 ng RNA with the PrimeScript RT Master Mix (Takara).

other:

Article Title: Inhibitory effect of cardamom seed hot-water extract on human influenza A virus infection
Article Snippet: Total RNA was isolated from cells using TRI Reagent (Merck).

Review