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Becton Dickinson spotlight random primer labeling kit
Spotlight Random Primer Labeling Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/random+primer+labelling+method/spotlight++random+primer+labeling+kit/pmc04902017-127-4-9
Average 90 stars, based on 1 article reviews
spotlight random primer labeling kit - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Labeling:

Article Title: Cornelia de Lange syndrome is caused by mutations in NIPBL , the human homolog of Drosophila melanogaster Nipped-B
Article Snippet: Northern-blot analysis We hybridized poly(A) + RNA northern blots of multiple adult human tissues (Human 12-Lane Multiple Tissue Northern (MTN) Blot BD Biosciences Clontech) and human fetal tissues (MessageMap Northern Blot, Stratagene) with a 301-bp probe from {"type":"entrez-nucleotide","attrs":{"text":"BX538178","term_id":"31874625","term_text":"BX538178"}} BX538178 -specific cDNA sequence ( NIPBL exon 2 and 3), a 344-bp probe from IDN3 -specific cDNA sequence ( NIPBL exon 46 and 47) and a 252-bp probe from a region of overlap between the two putative transcripts ( NIPBL exon 10; all primer sequences available on request). .. We used the BD SpotLight Random Primer Labeling Kit (BD Bioscience Clontech) to label probes and SpotLight Chemiluminescent Hybridization & Detection Kit (BD Bioscience Clontech) for hybridization and visualization. .. Experiments were duplicated using Ready-to-go DNA labeling beads (-dCTP; Amersham) with 32 P-dCTP and purified on ProbeQuant G-50 microcolumns (Amersham).

Hybridization:

Article Title: Cornelia de Lange syndrome is caused by mutations in NIPBL , the human homolog of Drosophila melanogaster Nipped-B
Article Snippet: Northern-blot analysis We hybridized poly(A) + RNA northern blots of multiple adult human tissues (Human 12-Lane Multiple Tissue Northern (MTN) Blot BD Biosciences Clontech) and human fetal tissues (MessageMap Northern Blot, Stratagene) with a 301-bp probe from {"type":"entrez-nucleotide","attrs":{"text":"BX538178","term_id":"31874625","term_text":"BX538178"}} BX538178 -specific cDNA sequence ( NIPBL exon 2 and 3), a 344-bp probe from IDN3 -specific cDNA sequence ( NIPBL exon 46 and 47) and a 252-bp probe from a region of overlap between the two putative transcripts ( NIPBL exon 10; all primer sequences available on request). .. We used the BD SpotLight Random Primer Labeling Kit (BD Bioscience Clontech) to label probes and SpotLight Chemiluminescent Hybridization & Detection Kit (BD Bioscience Clontech) for hybridization and visualization. .. Experiments were duplicated using Ready-to-go DNA labeling beads (-dCTP; Amersham) with 32 P-dCTP and purified on ProbeQuant G-50 microcolumns (Amersham).

other:

Article Title: Methods and compositions for the diagnosis of Cornelia de Lange Syndrome
Article Snippet: BD SpotLightTM Random Primer Labeling Kit (BD Bioscience Clontech) was used for labeling probes and SpotLightTM Chemiluminescent Hybridization & Detection Kit (BD Bioscience Clontech) was used for hybridization and visualization.



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Image Search Results


Differential expression of circRNA by A, scatter plot, B, volcanic map, and C, cluster analysis in normal group and diabetic cardiomyopathy (DCM) group. circRNA, circular RNA.

Journal: Journal of Diabetes

Article Title: CircMAP3K5 promotes cardiomyocyte apoptosis in diabetic cardiomyopathy by regulating miR ‐22‐3p/ DAPK2 Axis

doi: 10.1111/1753-0407.13471

Figure Lengend Snippet: Differential expression of circRNA by A, scatter plot, B, volcanic map, and C, cluster analysis in normal group and diabetic cardiomyopathy (DCM) group. circRNA, circular RNA.

Article Snippet: These circRNAs were amplified and transcribed into fluorescent cRNA using a random primer method (Arraystar Super RNA Labeling Kit; Arraystar). labeled cRNAs that were hybridized to Arraystar Rat circRNA Array V2.0 (8 × 15 K) with a total of 14 145 circRNA probes on the chip.

Techniques: Quantitative Proteomics

Depletion of circRNA Mitogen‐activated protein kinase kinase kinase 5 (circMAP3K5) attenuates cardiomyocyte apoptosis in a diabetic cardiomyopathy (DCM) cell model. A, quantitative real‐time polymerase chain reaction results show expression levels of circMAP3K5 in samples of DCM ( n = 3) and control ( n = 3) cells. B, Western blots showing expression levels of Bcl‐2, Bax, and cleaved caspase‐3 proteins in the samples. C, Flow cytometry results depict the rate of apoptosis. Statistically significant difference: * p < .05 ( n = 3). circRNA, circular RNA; HG, high glucose.

Journal: Journal of Diabetes

Article Title: CircMAP3K5 promotes cardiomyocyte apoptosis in diabetic cardiomyopathy by regulating miR ‐22‐3p/ DAPK2 Axis

doi: 10.1111/1753-0407.13471

Figure Lengend Snippet: Depletion of circRNA Mitogen‐activated protein kinase kinase kinase 5 (circMAP3K5) attenuates cardiomyocyte apoptosis in a diabetic cardiomyopathy (DCM) cell model. A, quantitative real‐time polymerase chain reaction results show expression levels of circMAP3K5 in samples of DCM ( n = 3) and control ( n = 3) cells. B, Western blots showing expression levels of Bcl‐2, Bax, and cleaved caspase‐3 proteins in the samples. C, Flow cytometry results depict the rate of apoptosis. Statistically significant difference: * p < .05 ( n = 3). circRNA, circular RNA; HG, high glucose.

Article Snippet: These circRNAs were amplified and transcribed into fluorescent cRNA using a random primer method (Arraystar Super RNA Labeling Kit; Arraystar). labeled cRNAs that were hybridized to Arraystar Rat circRNA Array V2.0 (8 × 15 K) with a total of 14 145 circRNA probes on the chip.

Techniques: Real-time Polymerase Chain Reaction, Expressing, Control, Western Blot, Flow Cytometry