random primer labelling method (Promega)
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Promega
random primer labelling method
Random Primer Labelling Method, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/random+primer+labelling+method/random+primer+labeling+system/pm18433440-65-2-6
Average 90 stars, based on 1 article reviews
Random Primer Labelling Method, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/random+primer+labelling+method/random+primer+labeling+system/pm18433440-65-2-6
Average 90 stars, based on 1 article reviews
random primer labelling method - by Bioz Stars,
2026-10
90/100 stars
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Amplification:Article Title: Artificial TALE as a Convenient Protein Platform for Engineering Broad-Spectrum Resistance to Begomoviruses Article Snippet: .. To further confirm these results, Southern blots were performed with a probe that amplified using primers Y35-F (5′-GATGCCTCAGCCAAGGAAAAC-3′) and Y35-R (5′-TCAACACGACGACGTCTG-3′) from the TbCSV genome and labeled with [α −32 P] dCTP using the Labeling:Article Title: Artificial TALE as a Convenient Protein Platform for Engineering Broad-Spectrum Resistance to Begomoviruses Article Snippet: .. To further confirm these results, Southern blots were performed with a probe that amplified using primers Y35-F (5′-GATGCCTCAGCCAAGGAAAAC-3′) and Y35-R (5′-TCAACACGACGACGTCTG-3′) from the TbCSV genome and labeled with [α −32 P] dCTP using the Article Title: Involvement of iNOS and NO in TNF-alpha-downregulated resistin gene expression in 3T3-L1 adipocytes. Article Snippet: objective: In order to characterize the regulation of resistin gene expression, we explore the effect of tumor necrosis factor-α (TNF-α) on resistin mRNA expression and its underlying mechanism in 3T3-L1 adipocytes.. Methods and Procedures: Differentiated 3T3-L1 adipocytes were treated for 24 h with 0–10 ng/ml of TNF-α or with 2.5 ng/ml of TNF-α for 0–24 h, and then resistin mRNA levels were measured by northern blotting.. To further explore the involvement of nitric oxide (NO) in TNF-α–regulated resistin expression, the effect of the NO donor, sodium nitroprusside (SNP), on resistin mRNA levels in adipocytes and the effect of the nitric oxide synthase (NOS) inhibitors, NG-nitro-l-arginine methyl ester (l-NAME), and S,Sʹ-1,3-phenylene-bis(1,2-ethanediyl)-bis-isothiourea·2HBr (PBITU), on the TNF-α effect in adipocytes were examined. Article Title: Characterization of phosphatidylinositol-glycan biosynthesis protein class F gene in rice. Article Snippet: The glycosylphosphatidylinositol (GPI) anchors are linked to glycosylphosphatidylinositol-anchored proteins (GAPs) which are essential for the growth of mammalian, yeast and protozoan cells.. The GPI anchor is covalently linked to GAP by amide bond formation between the carboxyl terminus and phosphoethanolamine attached at the third mannose and mediated by a transamidase complex.. Mediation of GPI synthesis is by the sequential additions of GPI–N-acetylglucosaminyltransferase (GPI–GnT) complex, the GlcN-PI de-N-acetylase, the GlcN-PI mannosyltransferases and the GPI lipid anchor phosphoethanolamine transferase complexes. Random Primed:Article Title: Artificial TALE as a Convenient Protein Platform for Engineering Broad-Spectrum Resistance to Begomoviruses Article Snippet: .. To further confirm these results, Southern blots were performed with a probe that amplified using primers Y35-F (5′-GATGCCTCAGCCAAGGAAAAC-3′) and Y35-R (5′-TCAACACGACGACGTCTG-3′) from the TbCSV genome and labeled with [α −32 P] dCTP using the Transgenic Assay:Article Title: Artificial TALE as a Convenient Protein Platform for Engineering Broad-Spectrum Resistance to Begomoviruses Article Snippet: .. To further confirm these results, Southern blots were performed with a probe that amplified using primers Y35-F (5′-GATGCCTCAGCCAAGGAAAAC-3′) and Y35-R (5′-TCAACACGACGACGTCTG-3′) from the TbCSV genome and labeled with [α −32 P] dCTP using the other:Article Title: Decapping enzyme 1A breaks X-chromosome symmetry by controlling Tsix elongation and RNA turnover Article Snippet: For Tsix detection, a 816 bp PCR fragment encompassing Tsix Exons 2 and 3 (primers Tsix-NB-f and Tsix-NB-r) was random-primed labeled ( Article Title: Suppression of RNA Silencing by a Plant DNA Virus Satellite Requires a Host Calmodulin-Like Protein to Repress RDR6 Expression Article Snippet: Activity Assay:Article Title: Acute-phase serum amyloid A production by rheumatoid arthritis synovial tissue Article Snippet: Of total RNA, 10 μg was electrophoresed on a standard northern gel and transferred onto a nylon membrane (BioRad, Richmond, CA, USA). .. The human A-SAA cDNA [ ] was radiolabelled to a high specific activity using [α- 32 P] dCTP and a Hybridization:Article Title: Involvement of iNOS and NO in TNF-alpha-downregulated resistin gene expression in 3T3-L1 adipocytes. Article Snippet: objective: In order to characterize the regulation of resistin gene expression, we explore the effect of tumor necrosis factor-α (TNF-α) on resistin mRNA expression and its underlying mechanism in 3T3-L1 adipocytes.. Methods and Procedures: Differentiated 3T3-L1 adipocytes were treated for 24 h with 0–10 ng/ml of TNF-α or with 2.5 ng/ml of TNF-α for 0–24 h, and then resistin mRNA levels were measured by northern blotting.. To further explore the involvement of nitric oxide (NO) in TNF-α–regulated resistin expression, the effect of the NO donor, sodium nitroprusside (SNP), on resistin mRNA levels in adipocytes and the effect of the nitric oxide synthase (NOS) inhibitors, NG-nitro-l-arginine methyl ester (l-NAME), and S,Sʹ-1,3-phenylene-bis(1,2-ethanediyl)-bis-isothiourea·2HBr (PBITU), on the TNF-α effect in adipocytes were examined. Generated:Article Title: Characterization of phosphatidylinositol-glycan biosynthesis protein class F gene in rice. Article Snippet: The glycosylphosphatidylinositol (GPI) anchors are linked to glycosylphosphatidylinositol-anchored proteins (GAPs) which are essential for the growth of mammalian, yeast and protozoan cells.. The GPI anchor is covalently linked to GAP by amide bond formation between the carboxyl terminus and phosphoethanolamine attached at the third mannose and mediated by a transamidase complex.. Mediation of GPI synthesis is by the sequential additions of GPI–N-acetylglucosaminyltransferase (GPI–GnT) complex, the GlcN-PI de-N-acetylase, the GlcN-PI mannosyltransferases and the GPI lipid anchor phosphoethanolamine transferase complexes. |
